FastQCFastQC Report
Thu 26 May 2016
SRR1513135_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513135_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2195713
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22270.10142491300092499No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTT350.002153803816.3046445
CGCAGAT1457.2759576E-1212.4309488
CTACACG855.1507348E-511.2130224
TACCGAA600.005938210511.0679057
GTCCTAC12650.010.65061951
TAGGACC6650.010.6060014
TGAGCGA909.635787E-510.54278410
GTCTTAC1803.5470293E-1010.0861951
GTCCTAA7400.010.0718481
TGTAGGA14850.09.89108852
CTAGTAC1553.8658982E-89.8382973
GTAGGAC14100.09.8012293
AGGACGT11800.09.7528485
GGACGTG11300.09.7384166
TCCTATA3550.09.6721722
GGCGAGG5800.09.6525419
AGGACTG4650.09.6133115
TGGCGAG12400.09.56546118
GTATTAA3600.09.5553431
ATTATAC2201.6370905E-119.530853
Produced by FastQC (version 0.11.5)