##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513135_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2195713
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.454208723999905	31.0	30.0	34.0	25.0	34.0
2	29.276455074046563	31.0	30.0	34.0	22.0	34.0
3	29.489706532684373	31.0	30.0	34.0	23.0	34.0
4	32.5384560732664	37.0	33.0	37.0	22.0	37.0
5	32.7163454422322	37.0	33.0	37.0	25.0	37.0
6	33.07731611553969	37.0	35.0	37.0	27.0	37.0
7	33.044571854336155	37.0	35.0	37.0	26.0	37.0
8	32.92434894724401	37.0	35.0	37.0	26.0	37.0
9	34.15928174583837	39.0	35.0	39.0	25.0	39.0
10	33.73361363711924	39.0	34.0	39.0	19.0	39.0
11	34.19597278879343	39.0	35.0	39.0	26.0	39.0
12	34.1855829974136	39.0	35.0	39.0	25.0	39.0
13	34.22940111025439	39.0	35.0	39.0	25.0	39.0
14	34.439740530752424	39.0	34.0	41.0	17.0	41.0
15	34.88528965306486	40.0	34.0	41.0	22.0	41.0
16	34.73552827714733	39.0	34.0	41.0	18.0	41.0
17	34.34619597369966	39.0	34.0	41.0	17.0	41.0
18	34.071518909802876	39.0	33.0	41.0	17.0	41.0
19	34.70534036096703	39.0	34.0	41.0	18.0	41.0
20	34.92764673707356	40.0	35.0	41.0	22.0	41.0
21	34.97099757573053	40.0	35.0	41.0	22.0	41.0
22	34.64892770594336	40.0	35.0	41.0	18.0	41.0
23	34.19864344748152	39.0	34.0	41.0	10.0	41.0
24	34.45654600578491	40.0	34.0	41.0	17.0	41.0
25	34.105827127680165	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9319182272422282
1101	2	1.225940189069032
1101	3	1.188984666457916
1101	4	1.468658037204996
1101	5	1.4437534970558659
1101	6	1.066797562066995
1101	7	0.990473454627292
1101	8	1.2244863752745303
1101	9	1.5116496109648168
1101	10	1.8369100973125754
1101	11	1.465423242404242
1101	12	1.331406739188786
1101	13	1.2426158505861125
1101	14	2.238610478558037
1101	15	1.844815783271713
1101	16	1.911303813150802
1101	17	2.225554817276972
1101	18	2.3701520396132594
1101	19	1.7994829781466706
1101	20	1.4926519397191882
1101	21	1.5234363273264364
1101	22	1.7665174962632975
1101	23	2.1434868204405007
1101	24	1.7166810587515258
1101	25	2.077830566268659
1102	1	0.8585174011732981
1102	2	1.1128422166441467
1102	3	1.023631525060516
1102	4	1.338751160619914
1102	5	1.2949593710949117
1102	6	0.9519231313982885
1102	7	1.0001276969811244
1102	8	1.0696277679203376
1102	9	1.3803673064341524
1102	10	1.515433850211359
1102	11	1.1195324807200393
1102	12	1.109306374448316
1102	13	1.1133707878940555
1102	14	2.0819241080218873
1102	15	1.7310129126910923
1102	16	1.821906444123492
1102	17	2.161185443115997
1102	18	2.4356167444900194
1102	19	1.8635082724716128
1102	20	1.608765711489859
1102	21	1.489432939615817
1102	22	1.717886159484415
1102	23	1.9711773038895046
1102	24	1.8316121427495915
1102	25	2.0916392210353507
1103	1	0.6865186791625959
1103	2	0.8947808554080474
1103	3	0.7206471833877117
1103	4	1.1211302915280328
1103	5	0.9055829529557471
1103	6	0.7989487000591566
1103	7	0.7243435233402451
1103	8	0.7362367475701319
1103	9	1.1502662210307406
1103	10	1.3595176768426427
1103	11	1.0265232596722598
1103	12	0.8719053668484165
1103	13	0.9623177779630083
1103	14	1.7123648072477025
1103	15	1.2862619874800885
1103	16	1.2901753307048978
1103	17	1.6476570980825542
1103	18	1.931788989047476
1103	19	1.403416446573985
1103	20	0.9746268307363195
1103	21	1.0501510394350646
1103	22	1.348899883077813
1103	23	1.5991427863452543
1103	24	1.3409118815309498
1103	25	1.5662007257494182
1104	1	0.8305215387839553
1104	2	1.0997363699873226
1104	3	0.9194327161447617
1104	4	1.3210797250527335
1104	5	1.1304328882219465
1104	6	0.9312611136929192
1104	7	0.8175889756268546
1104	8	0.9129535012024164
1104	9	1.1712199221915895
1104	10	1.4537151738021166
1104	11	0.9498758169845658
1104	12	0.8950238362535217
1104	13	0.8483191467451832
1104	14	1.8264924701611136
1104	15	1.475779905884977
1104	16	1.5060052529807422
1104	17	1.9456069762435035
1104	18	1.9600512895605462
1104	19	1.403594301073312
1104	20	1.196978084654134
1104	21	1.251045542946379
1104	22	1.528501541309467
1104	23	1.7076872129170653
1104	24	1.4490256212269372
1104	25	1.7779042492737247
1105	1	0.6834130648674055
1105	2	0.9079593415294553
1105	3	0.815573032728139
1105	4	1.082519928209308
1105	5	1.0670896354019206
1105	6	0.7305436870323945
1105	7	0.7463238509816819
1105	8	0.8005512278652631
1105	9	0.9768442797183567
1105	10	1.332748876908532
1105	11	0.8714072759893128
1105	12	0.8037439824137707
1105	13	0.6434521681762533
1105	14	1.468788235474804
1105	15	1.0143317294158507
1105	16	1.3215756749061498
1105	17	1.6455069240311673
1105	18	1.294199958403162
1105	19	1.142678364650621
1105	20	0.9526586411742031
1105	21	0.8590569216350019
1105	22	1.0043136832069948
1105	23	1.303532002528783
1105	24	1.1369947499324908
1105	25	1.3893835902494374
1106	1	0.5181314397519863
1106	2	0.7078977841271765
1106	3	0.599814337745066
1106	4	1.0791342710840368
1106	5	0.978139189110756
1106	6	0.6105067525910286
1106	7	0.6105897789595218
1106	8	0.6574302675697865
1106	9	0.846034799878403
1106	10	0.872607294883295
1106	11	0.6279477499813098
1106	12	0.6661631883232815
1106	13	0.5388045843042875
1106	14	1.5860550868136798
1106	15	1.1226727574244677
1106	16	1.2658662258448956
1106	17	1.5722536153207969
1106	18	1.5936767204837992
1106	19	1.075273009156625
1106	20	0.9203410049784466
1106	21	0.8242769893481423
1106	22	1.0384780414710733
1106	23	1.451269767995079
1106	24	0.9575549222932196
1106	25	1.2838126453433105
1107	1	0.7753496664926196
1107	2	0.971529183076175
1107	3	0.8593957270079073
1107	4	1.2061336463292633
1107	5	1.0357826237467265
1107	6	0.8497519427350539
1107	7	0.7389917690125998
1107	8	0.9361003865699473
1107	9	1.093245822047507
1107	10	1.235715845399362
1107	11	0.8901339688201944
1107	12	0.845443882937083
1107	13	0.8143409140679836
1107	14	1.663760503577599
1107	15	1.1011762596956842
1107	16	1.2853459257660091
1107	17	1.556831807549223
1107	18	1.8255247287846856
1107	19	1.3535290669920172
1107	20	1.2028389409322315
1107	21	1.044888221524623
1107	22	1.3384242274298899
1107	23	1.6120288065608221
1107	24	1.2537966286666347
1107	25	1.4878784941441197
1108	1	0.5637705706196598
1108	2	0.7611368446175533
1108	3	0.48185226056008545
1108	4	0.9234030450688024
1108	5	0.8581278960097762
1108	6	0.48290789590384264
1108	7	0.41359718085070796
1108	8	0.5257874441672428
1108	9	0.6248704193372703
1108	10	0.9804671385893258
1108	11	0.5747663370434353
1108	12	0.2725798882918653
1108	13	0.4201495544416787
1108	14	1.2957870211482074
1108	15	0.845782379155601
1108	16	1.0084736319187115
1108	17	1.2796975601228056
1108	18	1.4843898725364895
1108	19	0.7673191472025067
1108	20	0.68694597785273
1108	21	0.7128880061227605
1108	22	0.7871377131437143
1108	23	1.2607572457919076
1108	24	0.921938827714861
1108	25	1.1465771910074025
1109	1	0.365517877605285
1109	2	0.6887219758566978
1109	3	0.5745709507256933
1109	4	0.8144437581563437
1109	5	0.8775230474855178
1109	6	0.5650414293478363
1109	7	0.5566201229870344
1109	8	0.5450583466071492
1109	9	0.6781277387390148
1109	10	1.0874061924162177
1109	11	0.5731043524155197
1109	12	0.6521055979129073
1109	13	0.5258874170946441
1109	14	1.5271878455502232
1109	15	0.9763729487064978
1109	16	1.1115354831590523
1109	17	1.5187399638407868
1109	18	1.5873237841755667
1109	19	1.0088758804194455
1109	20	0.8547380619413829
1109	21	0.7609965474092064
1109	22	1.0287938280151963
1109	23	1.3814737075589676
1109	24	1.0982048893631244
1109	25	1.3430642999502567
1110	1	0.33737583611481625
1110	2	0.4933230719295949
1110	3	0.4622130604559196
1110	4	0.7463448205781802
1110	5	0.6388905236180946
1110	6	0.32056303287449595
1110	7	0.26499482558844534
1110	8	0.25170257229078885
1110	9	0.5925460529704267
1110	10	0.8032837956414411
1110	11	0.43331720489644
1110	12	0.3463307085463967
1110	13	0.22972443624310301
1110	14	1.1677666089902417
1110	15	0.7602743042293909
1110	16	0.8634794480730505
1110	17	1.1472692876629225
1110	18	0.7348706430109502
1110	19	0.5421654222337438
1110	20	0.345518575444693
1110	21	0.21095423181369455
1110	22	0.46863518533697857
1110	23	-0.647854629165515
1110	24	0.15605813950298852
1110	25	0.555062481612481
1111	1	0.11575882535655424
1111	2	0.4441307960286025
1111	3	0.31151254348084123
1111	4	0.7075960204914296
1111	5	0.5748240380265699
1111	6	0.30540948186371963
1111	7	0.20759102617606118
1111	8	0.33281670805605756
1111	9	0.48945462517931304
1111	10	0.6865357091463267
1111	11	0.4368345650586605
1111	12	0.2527762022186195
1111	13	0.15081211287934337
1111	14	1.0534461315876769
1111	15	0.6831341733885381
1111	16	0.7366247286275467
1111	17	1.1399806512981385
1111	18	1.4397675014061164
1111	19	0.9124725006821848
1111	20	0.4214540363183019
1111	21	0.16363787010726583
1111	22	0.6090646469389966
1111	23	1.0147784262914001
1111	24	0.6185599769424996
1111	25	0.8566842608735641
1112	1	0.1592141325135934
1112	2	0.2184509002975048
1112	3	0.14413255932223024
1112	4	0.641333887922066
1112	5	0.32767396540216964
1112	6	0.18724357800154934
1112	7	0.0671723627613332
1112	8	0.14912357033399104
1112	9	0.34887077391063315
1112	10	0.6641790102337666
1112	11	0.1392887156510696
1112	12	0.17213581925575028
1112	13	-0.04207662074313134
1112	14	0.6462406790070787
1112	15	0.39682875411180873
1112	16	0.4032651538651848
1112	17	0.7965255605080657
1112	18	0.9572546121765129
1112	19	0.3567065791112114
1112	20	0.2882847479775421
1112	21	0.13542727980992453
1112	22	0.5015525690855043
1112	23	0.7537607293046733
1112	24	0.5332044585613787
1112	25	0.8489058685766295
1113	1	-0.22550701380801286
1113	2	-0.035994435607449304
1113	3	-0.10511304770967911
1113	4	0.13698768857392452
1113	5	0.09423602818031185
1113	6	-0.18093987798445
1113	7	-0.27369600760493995
1113	8	-0.25360584680892373
1113	9	-0.09880043920464487
1113	10	0.17000254189291297
1113	11	-0.14282444757579071
1113	12	-0.21340987314557935
1113	13	-0.38759057627198956
1113	14	0.23833244718166924
1113	15	-0.11670042167805406
1113	16	0.02306838920812737
1113	17	0.4269006721602011
1113	18	0.31916759860435917
1113	19	0.011939770689153306
1113	20	-0.24895239193609342
1113	21	-0.36325067986092563
1113	22	-0.05515798773917879
1113	23	0.09195636492479764
1113	24	-0.046348017283555976
1113	25	0.31402346890546085
1114	1	-0.9752456783529801
1114	2	-0.724731803206982
1114	3	-0.6512089666040666
1114	4	-0.38221900363920724
1114	5	-3.360828515268313
1114	6	-1.2909799858163815
1114	7	-1.0411607799337332
1114	8	-0.7170146897107941
1114	9	-0.37222877110114183
1114	10	-0.16365431571399824
1114	11	-0.5500941938707271
1114	12	-0.62830518705195
1114	13	-0.6070672597375477
1114	14	-6.246976987289351
1114	15	-2.5991387265722707
1114	16	-1.2674229404362904
1114	17	-0.39809856828171064
1114	18	-6.316663225022456
1114	19	-2.4036196027007435
1114	20	-1.416620409350223
1114	21	-1.1657475371893327
1114	22	-0.7629451513340655
1114	23	-0.3752334157274859
1114	24	-0.4610319999757593
1114	25	-0.5474224543563864
1115	1	-0.6201551220577635
1115	2	-0.18330384122374355
1115	3	-0.25801709178986343
1115	4	-2.7314656715452514
1115	5	-0.9098255210314008
1115	6	-0.4625985501441576
1115	7	-0.4772514402302903
1115	8	-0.29441938190954176
1115	9	-0.060944507741467646
1115	10	-0.4516070714107201
1115	11	-0.5410367229653232
1115	12	-0.527412027659139
1115	13	-0.606082635142613
1115	14	-5.990627381330693
1115	15	-2.268148713352609
1115	16	-0.979365446845037
1115	17	-0.19281494660735632
1115	18	-0.02868807810214946
1115	19	-0.301249285449515
1115	20	-0.3846746149670608
1115	21	-0.5079779446477346
1115	22	-1.8446686244367498
1115	23	-0.8409231375960786
1115	24	-0.5206160771052737
1115	25	-0.16321675207775854
1116	1	-0.2843815065774251
1116	2	0.017697113726679703
1116	3	-0.07282220373244641
1116	4	0.19893677033904567
1116	5	-0.4538691762097855
1116	6	-0.1884762805734823
1116	7	-0.1381009024368609
1116	8	-0.12220532301107312
1116	9	-0.0896107145389351
1116	10	0.03345254960917288
1116	11	-0.24688558304607255
1116	12	-0.5108274226431107
1116	13	-0.421340133721813
1116	14	0.41139169443786017
1116	15	0.005291939225017472
1116	16	0.03789892653212945
1116	17	0.4601464032530984
1116	18	0.36746343999437414
1116	19	-0.009851286619962707
1116	20	-0.28640688869980124
1116	21	-0.412581407821925
1116	22	-0.3378210277638658
1116	23	-1.0317307362126655
1116	24	-0.7591437315822347
1116	25	-0.004730105538286011
1201	1	0.9030750717818776
1201	2	1.0128199426446471
1201	3	1.0564291100900434
1201	4	1.3265661105766853
1201	5	1.240696684440266
1201	6	0.9685873068939586
1201	7	0.8614691192932256
1201	8	0.902224300796469
1201	9	1.1661063782531471
1201	10	1.630358016035423
1201	11	1.1267176040330042
1201	12	1.1148241716669958
1201	13	0.9487442437343816
1201	14	1.8287850860263148
1201	15	1.5088801772387939
1201	16	1.6833914751165864
1201	17	1.9782710661285563
1201	18	1.390061726245861
1201	19	1.3416527896973278
1201	20	1.3483779680511674
1201	21	1.3626291120048464
1201	22	1.4915078824955046
1201	23	1.7852169574701975
1201	24	1.3417169184709365
1201	25	1.6538012643817268
1202	1	0.7945596680231866
1202	2	0.9064919584345716
1202	3	0.9041046728921849
1202	4	1.198100765458122
1202	5	0.9866436508866698
1202	6	0.79924407625235
1202	7	0.7209416442510275
1202	8	0.7853722741366624
1202	9	1.217720268810659
1202	10	1.458986981394844
1202	11	1.0487067913667332
1202	12	0.8728847569240088
1202	13	0.9390177069341306
1202	14	1.8316038165392001
1202	15	1.3931786411536677
1202	16	1.4370486163391618
1202	17	1.821930495746109
1202	18	1.8801795785871747
1202	19	1.396418658286244
1202	20	1.1473390367316227
1202	21	1.0371772970215787
1202	22	1.2671441993237877
1202	23	1.5050067111816858
1202	24	0.9795443734896665
1202	25	1.491561211748639
1203	1	0.648528553015904
1203	2	0.8041177855215729
1203	3	0.6936315598737615
1203	4	1.0753396483441904
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2211	13	-0.672199470455034
2211	14	-0.17042623854396055
2211	15	-0.4961956569371395
2211	16	-7.257929724748596
2211	17	-2.282065974653378
2211	18	-0.8412830664740625
2211	19	-0.7689302908554225
2211	20	-0.9679954731352183
2211	21	-0.9025141303274751
2211	22	-0.7607401795199991
2211	23	-0.33150700612939943
2211	24	-0.7481988213335811
2211	25	-8.060219632146755
2212	1	-1.492132103420694
2212	2	-3.8364793128622985
2212	3	-1.7395613726791623
2212	4	-3.6632131729995088
2212	5	-1.2907187295263824
2212	6	-1.0806483542136789
2212	7	-1.0430471795127403
2212	8	-0.8020437605611619
2212	9	-0.8709621392298317
2212	10	-0.37479225314432085
2212	11	-0.8822641180594744
2212	12	-0.921772431514917
2212	13	-1.0586476183754172
2212	14	-6.2066843612506375
2212	15	-4.9124099119030085
2212	16	-2.7287638267923384
2212	17	-7.46164779618957
2212	18	-3.025155317053155
2212	19	-1.9567330090154371
2212	20	-2.0044573553482863
2212	21	-1.4943826091008177
2212	22	-1.3857566811701645
2212	23	-0.8670493057009736
2212	24	-1.2716036959825416
2212	25	-0.979164756472855
2213	1	-0.10809796094781632
2213	2	-0.11483367183246429
2213	3	-0.09584276464990182
2213	4	0.37765259813581764
2213	5	0.1587153633461611
2213	6	-0.21854818878838245
2213	7	-0.3325354512135661
2213	8	-0.33386401112461783
2213	9	-0.1318604503656502
2213	10	-3.7769763030934946
2213	11	-1.4170631140614702
2213	12	-0.8925780009240469
2213	13	-0.7554113327319882
2213	14	0.046041885775387925
2213	15	-0.4232404995034713
2213	16	-0.14379599887825378
2213	17	0.19694382680427225
2213	18	0.39894336036206823
2213	19	-0.21682626854782683
2213	20	-0.45475046062908575
2213	21	-0.4597773814434518
2213	22	-0.7565508043479667
2213	23	-1.0848367491852002
2213	24	-0.5226980374563226
2213	25	-0.19662025924856152
2214	1	-0.8800585316787881
2214	2	-0.6111065119236017
2214	3	-0.5774591010222601
2214	4	-0.1600623337048077
2214	5	-3.31783805389232
2214	6	-1.4153404962038643
2214	7	-1.0635395724013108
2214	8	-0.7891599016009678
2214	9	-0.5866831183871213
2214	10	-4.17986181535624
2214	11	-1.8840923776143512
2214	12	-1.2424920016274825
2214	13	-1.0087153793937418
2214	14	-0.238125243078116
2214	15	-0.4905729097350431
2214	16	-0.5137289928231752
2214	17	-0.14296224845178784
2214	18	0.015905284172575307
2214	19	-0.6011580937791834
2214	20	-0.8471712100832676
2214	21	-0.9108617042980356
2214	22	-0.6597544043999477
2214	23	-0.442216516826619
2214	24	-0.867835222214417
2214	25	-0.5699261135078686
2215	1	-2.1728691142646355
2215	2	-4.757883933324347
2215	3	-2.794795696111507
2215	4	-1.8385191607411429
2215	5	-1.9691575791256284
2215	6	-2.0563677318657305
2215	7	-2.2570549117145298
2215	8	-2.4009012805988803
2215	9	-8.059716877560806
2215	10	-3.777412526600237
2215	11	-2.928451388215059
2215	12	-3.290589781821378
2215	13	-2.7939038182811196
2215	14	-8.032188557384536
2215	15	-4.671882613458877
2215	16	-9.066022478371785
2215	17	-4.392674670305553
2215	18	-3.034025095459569
2215	19	-3.479910001000345
2215	20	-3.194592574584494
2215	21	-3.3672561593129835
2215	22	-9.080206846186666
2215	23	-10.321350484390269
2215	24	-6.373879980753873
2215	25	-4.593008037943857
2216	1	-1.8162047985635432
2216	2	-1.7428014816031023
2216	3	-1.6106714307882477
2216	4	-6.798129572160118
2216	5	-3.429976386850228
2216	6	-2.482449343956411
2216	7	-2.041371956214949
2216	8	-7.58853117144691
2216	9	-3.7406196483416636
2216	10	-2.043533769253468
2216	11	-2.0715183907339494
2216	12	-2.2950116122160047
2216	13	-2.0177439526220127
2216	14	-1.3963846950839525
2216	15	-1.9868166184966611
2216	16	-1.720197457381019
2216	17	-1.4272943176607171
2216	18	-7.6123720308080145
2216	19	-4.631306810404208
2216	20	-3.976512977607161
2216	21	-3.0856601895396807
2216	22	-2.7314034876644158
2216	23	-1.867909006640481
2216	24	-2.2142762863793948
2216	25	-1.615649851545868
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101495.0
3	2.0
4	838.0
5	5057.0
6	5992.0
7	5429.0
8	4844.0
9	4792.0
10	4545.0
11	4262.0
12	4130.0
13	4011.0
14	4237.0
15	4202.0
16	4227.0
17	4540.0
18	5171.0
19	6353.0
20	8090.0
21	10200.0
22	12848.0
23	15279.0
24	17422.0
25	20368.0
26	22702.0
27	25687.0
28	28739.0
29	34120.0
30	41432.0
31	49167.0
32	62742.0
33	82164.0
34	108606.0
35	145926.0
36	207942.0
37	302545.0
38	825607.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.318413228358814	18.62624019285578	12.386165955790805	25.669180622994602
2	14.365888310778505	20.514261992847423	41.299565557124005	23.820284139250063
3	18.291614166629753	25.128454784053016	29.957514359397557	26.622416689919675
4	11.364565175307845	17.06956984557679	39.30165177870659	32.26421320040877
5	12.551951779432644	38.046718127146626	36.4405227761259	12.960807317294822
6	31.364392527281108	37.28278516024292	18.599621371553308	12.753200940922666
7	27.491997665919467	31.635719449350137	22.94787447894294	17.92440840578746
8	24.872923722347615	36.33695996283027	21.305723272302405	17.484393042519706
9	26.479387782990937	14.992711702273038	20.388602924429463	38.13929759030656
10	13.831972813906452	28.80245440134108	35.87958492009985	21.48598786465261
11	34.01530018202789	22.48112758897775	24.91609224932901	18.587479979665353
12	22.934020683368434	24.874937189563834	31.938679702992623	20.25236242407511
13	28.666828845179655	21.92038492796723	25.91302858350736	23.499757643345763
14	20.405292380131527	21.7412338804167	29.004817880824866	28.848655858626906
15	23.182619448237464	30.21512916087462	23.676737191780102	22.92551419910781
16	22.18977849488032	28.014161459220666	27.73997906392609	22.05608098197293
17	21.534431069518227	28.156659771644144	28.336765657320417	21.97214350151721
18	21.402847626514735	27.427846931760563	29.7885988334508	21.380706608273908
19	23.179836110083585	27.10046594008925	27.70134096719468	22.01835698263248
20	23.54043644997845	27.267736759671884	27.827763437587983	21.364063352761683
21	23.233281350585592	26.86888456686435	27.064377967880038	22.83345611467002
22	23.060727106877174	27.69933564278076	27.315693383264538	21.924243867077525
23	22.289630854596894	27.432838132724697	27.831003111518083	22.446527901160326
24	22.890818010576552	27.228516776584684	27.87025451708825	22.010410695750515
25	22.779343518698226	27.0893073714961	27.746554079111984	22.384795030693684
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	252.0
1	252.0
2	378.5
3	505.0
4	505.0
5	505.0
6	978.0
7	1451.0
8	1451.0
9	1451.0
10	2317.0
11	3183.0
12	3183.0
13	3183.0
14	6292.0
15	9401.0
16	9401.0
17	9401.0
18	17606.5
19	25812.0
20	25812.0
21	25812.0
22	41379.5
23	56947.0
24	56947.0
25	56947.0
26	81032.5
27	105118.0
28	105118.0
29	105118.0
30	134771.5
31	164425.0
32	164425.0
33	164425.0
34	188654.0
35	212883.0
36	212883.0
37	212883.0
38	232542.5
39	252202.0
40	252202.0
41	252202.0
42	264524.5
43	276847.0
44	276847.0
45	276847.0
46	283669.5
47	290492.0
48	290492.0
49	290492.0
50	277833.5
51	265175.0
52	265175.0
53	265175.0
54	235688.0
55	206201.0
56	206201.0
57	206201.0
58	176243.5
59	146286.0
60	146286.0
61	146286.0
62	118749.0
63	91212.0
64	91212.0
65	91212.0
66	70439.5
67	49667.0
68	49667.0
69	49667.0
70	36235.0
71	22803.0
72	22803.0
73	22803.0
74	16113.5
75	9424.0
76	9424.0
77	9424.0
78	6526.0
79	3628.0
80	3628.0
81	3628.0
82	2435.0
83	1242.0
84	1242.0
85	1242.0
86	804.0
87	366.0
88	366.0
89	366.0
90	253.5
91	141.0
92	141.0
93	141.0
94	85.5
95	30.0
96	30.0
97	30.0
98	25.0
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17379320521397834
2	0.2738062761390036
3	0.27471714199442276
4	0.4833965094709554
5	0.2706182456450365
6	0.019173726256573605
7	0.01894600979271881
8	0.01721536466742238
9	0.018809379914405934
10	0.021541977480663457
11	0.020266765283076613
12	0.028054668346910547
13	0.027371518955346168
14	0.025367614073423986
15	0.040624617151695144
16	0.020904371381870034
17	0.0406701604444661
18	0.03151595859750341
19	0.026460653099926998
20	0.029102164080642597
21	0.034339642749302846
22	0.04372156106012034
23	0.029284337251726432
24	0.04768382753119374
25	0.03638909092399599
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2195713.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.34332069329574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.34618487910473	63.54921656602399
2	10.96375599466916	16.520915678188043
3	2.6038092045672845	5.885388957716047
4	0.8876218145426041	2.6750550010979395
5	0.3981455644012243	1.4998804470647338
6	0.21292081392370837	0.9625296699438904
7	0.1398555340749519	0.7376026248178876
8	0.0837090573818353	0.504553468420249
9	0.06691609761914567	0.4537512902216858
>10	0.27144154503609275	3.4879403557250597
>50	0.014202478398959321	0.7305027278580734
>100	0.009420444304465835	1.4457784170213202
>500	0.0013443813172139653	0.6613326152844773
>1k	6.721906586067343E-4	0.8855521806166621
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2227	0.10142491300092499	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.554329277095868E-5	4.554329277095868E-5
2	0.0	0.0	0.0	4.554329277095868E-5	4.554329277095868E-5
3	0.0	0.0	0.0	1.3662987831287605E-4	4.554329277095868E-5
4	0.0	0.0	0.0	1.3662987831287605E-4	4.554329277095868E-5
5	0.0	0.0	0.0	1.3662987831287605E-4	4.554329277095868E-5
6	0.0	0.0	0.0	1.3662987831287605E-4	9.108658554191736E-5
7	0.0	0.0	0.0	1.3662987831287605E-4	9.108658554191736E-5
8	0.0	0.0	0.0	1.8217317108383473E-4	9.108658554191736E-5
9	0.0	0.0	0.0	3.188030493967108E-4	9.108658554191736E-5
10	0.0	0.0	0.0	3.188030493967108E-4	9.108658554191736E-5
11	0.0	4.554329277095868E-5	0.0	3.6434634216766945E-4	9.108658554191736E-5
12	0.0	4.554329277095868E-5	0.0	3.6434634216766945E-4	9.108658554191736E-5
13	0.0	4.554329277095868E-5	0.0	4.554329277095868E-4	1.3662987831287605E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGTT	35	0.0021538038	16.304644	5
CGCAGAT	145	7.2759576E-12	12.430948	8
CTACACG	85	5.1507348E-5	11.213022	4
TACCGAA	60	0.0059382105	11.067905	7
GTCCTAC	1265	0.0	10.6506195	1
TAGGACC	665	0.0	10.606001	4
TGAGCGA	90	9.635787E-5	10.542784	10
GTCTTAC	180	3.5470293E-10	10.086195	1
GTCCTAA	740	0.0	10.071848	1
TGTAGGA	1485	0.0	9.8910885	2
CTAGTAC	155	3.8658982E-8	9.838297	3
GTAGGAC	1410	0.0	9.801229	3
AGGACGT	1180	0.0	9.752848	5
GGACGTG	1130	0.0	9.738416	6
TCCTATA	355	0.0	9.672172	2
GGCGAGG	580	0.0	9.65254	19
AGGACTG	465	0.0	9.613311	5
TGGCGAG	1240	0.0	9.565461	18
GTATTAA	360	0.0	9.555343	1
ATTATAC	220	1.6370905E-11	9.53085	3
>>END_MODULE