FastQCFastQC Report
Thu 26 May 2016
SRR1513135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2195713
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32680.14883548077549297No Hit
GTACATGGGAAGCAGTGGTATCAAC26510.12073526913581147No Hit
CCCATGTACTCTGCGTTGATACCAC25240.1149512709538997No Hit
GTCCTACAGTGGACATTTCTAAATT22740.10356544776116004No Hit
TATCAACGCAGAGTACTTTTTTTTT22310.10160708617200882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTGC802.9036413E-511.8605238
TGGCGAG12900.011.4031518
GGACGTG11400.011.1907766
TAGGACC5800.010.99883654
AGGACGT11950.010.9157265
GGCGAGG5650.010.91463719
TATGGCG13050.010.76350816
TCCAACG1605.5479177E-1010.6766618
GACGTGG5550.010.6023317
GGCGAGA8450.010.55396219
ATGGCGA13500.010.54532917
TAGGACG12200.010.5359544
TATTAGG2100.010.4298482
GACGTGA6750.010.4047257
GTAGGAC13700.010.1475693
CGTGAAA7300.010.13715659
ACTTCGC750.002675248310.1209798
GTCCTAT2950.010.0107811
ATATGGC23750.09.91038915
CCAACGA1151.04315895E-59.89983119
Produced by FastQC (version 0.11.5)