##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513135_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2195713
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.735422161275174	31.0	31.0	34.0	27.0	34.0
2	30.84689483552723	33.0	31.0	34.0	27.0	34.0
3	30.949993919970414	33.0	31.0	34.0	27.0	34.0
4	34.325749767843064	37.0	35.0	37.0	31.0	37.0
5	34.063606218116846	37.0	35.0	37.0	30.0	37.0
6	33.98312302199787	37.0	35.0	37.0	30.0	37.0
7	33.84461129482769	37.0	35.0	37.0	29.0	37.0
8	33.81303521908373	37.0	35.0	37.0	29.0	37.0
9	35.363153563329995	39.0	35.0	39.0	29.0	39.0
10	35.20967130039308	39.0	35.0	39.0	28.0	39.0
11	35.222513142655714	39.0	35.0	39.0	29.0	39.0
12	35.16284915196112	39.0	35.0	39.0	28.0	39.0
13	35.131052646680146	39.0	35.0	39.0	28.0	39.0
14	36.28225728954558	40.0	37.0	41.0	27.0	41.0
15	36.26616821050838	40.0	37.0	41.0	27.0	41.0
16	36.22837183183777	40.0	36.0	41.0	27.0	41.0
17	36.15989658029078	40.0	36.0	41.0	27.0	41.0
18	36.11756408966017	40.0	36.0	41.0	27.0	41.0
19	36.202468628641356	40.0	37.0	41.0	27.0	41.0
20	36.1705309391528	40.0	37.0	41.0	27.0	41.0
21	36.11241405411363	40.0	37.0	41.0	27.0	41.0
22	36.05646958413964	40.0	37.0	41.0	27.0	41.0
23	35.97815424875655	40.0	37.0	41.0	27.0	41.0
24	35.919966771613595	40.0	37.0	41.0	27.0	41.0
25	35.864269146286425	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4775923199806016
1101	2	0.4729540053435315
1101	3	0.4296511323507879
1101	4	0.6506612254584141
1101	5	0.6008466354622755
1101	6	0.48063429833055693
1101	7	0.5968877719523107
1101	8	0.44745509511511017
1101	9	0.4990791890097839
1101	10	0.549543353160125
1101	11	0.5867988088028966
1101	12	0.5386611074518868
1101	13	0.5714687969768946
1101	14	0.666283970453442
1101	15	0.662204097444743
1101	16	0.609470682377804
1101	17	0.6740722871263785
1101	18	0.6503532973393789
1101	19	0.6394218477253517
1101	20	0.6885278654930573
1101	21	0.6586227242852374
1101	22	0.6274945813163413
1101	23	0.6713335746911042
1101	24	0.5650391012205773
1101	25	0.628429534532124
1102	1	0.6104762695971715
1102	2	0.6303256632675591
1102	3	0.5506131217304677
1102	4	0.6904045264498251
1102	5	0.7385159095687186
1102	6	0.7082619282781408
1102	7	0.6603511880648725
1102	8	0.5836241454805133
1102	9	0.5921573406929284
1102	10	0.666312302856916
1102	11	0.6539082430282832
1102	12	0.7066926043259514
1102	13	0.7848550476892697
1102	14	0.8843573927721167
1102	15	0.7664750453599254
1102	16	0.8811593497508596
1102	17	0.9384986315584172
1102	18	0.9291309601597604
1102	19	0.9205636975595652
1102	20	0.9057444557272518
1102	21	0.9383635927490843
1102	22	1.0025016877435817
1102	23	0.8465379575194376
1102	24	1.0080044513708373
1102	25	0.8546378599171547
1103	1	0.32436937407575783
1103	2	0.3039413967357909
1103	3	0.33297923608092717
1103	4	0.4731055213289892
1103	5	0.31792815282451414
1103	6	0.22524143607476788
1103	7	0.2967732913630954
1103	8	0.35092891930302983
1103	9	0.32604003192539466
1103	10	0.3391366887860201
1103	11	0.3267015667175315
1103	12	0.32374281569709495
1103	13	0.3897548941530502
1103	14	0.3863684509295098
1103	15	0.27381224224424017
1103	16	0.31410928629514245
1103	17	0.46919640779787386
1103	18	0.4269790865150398
1103	19	0.38380730539434893
1103	20	0.5154790658665647
1103	21	0.3710499440008945
1103	22	0.2673865651660634
1103	23	0.3623515959135517
1103	24	0.36912547281561814
1103	25	0.3627110839705665
1104	1	0.26630511106033694
1104	2	0.35415477854319377
1104	3	0.3674559820410579
1104	4	0.46154410703698545
1104	5	0.4032468741901525
1104	6	0.4459919994986379
1104	7	0.45806988553661654
1104	8	0.4695168940721004
1104	9	0.4300822985240558
1104	10	0.5268952425848283
1104	11	0.6072583210472828
1104	12	0.6070345188718846
1104	13	0.5941332534452997
1104	14	0.76031712595708
1104	15	0.6415647803761573
1104	16	0.6784015082122181
1104	17	0.693285230427243
1104	18	0.6594166085833777
1104	19	0.6817257974671804
1104	20	0.7553681683552185
1104	21	0.7071757673408072
1104	22	0.5226783903978642
1104	23	0.6349920813517116
1104	24	0.5810181847044049
1104	25	0.49179508970724584
1105	1	0.35625350743856643
1105	2	0.40577554722196396
1105	3	0.2571379154578963
1105	4	0.4132309770488476
1105	5	0.34593590385516393
1105	6	0.513904903490527
1105	7	0.4391937110805344
1105	8	0.5095120586438497
1105	9	0.5278685468747355
1105	10	0.41884485467888055
1105	11	0.3844788242919108
1105	12	0.4377696668625646
1105	13	0.5181081469415574
1105	14	0.4691146046756245
1105	15	0.5438376888605347
1105	16	0.4461629192656318
1105	17	0.4364313966785218
1105	18	0.4682251470580425
1105	19	0.517652172070548
1105	20	0.5796903724874483
1105	21	0.6025161806327475
1105	22	0.4901541339521245
1105	23	0.611767450846159
1105	24	0.5174597381800439
1105	25	0.6512939198593983
1106	1	0.2599161728740853
1106	2	0.31343944380552813
1106	3	0.26452480373120935
1106	4	0.3011965049035723
1106	5	0.36040189339040296
1106	6	0.2627507021978204
1106	7	0.35301334789186
1106	8	0.36669888535976014
1106	9	0.4552308121871391
1106	10	0.4120735404283451
1106	11	0.33369396741287005
1106	12	0.3814446437785435
1106	13	0.2718785378310642
1106	14	0.4127895815915963
1106	15	0.4508860114213178
1106	16	0.43138914271899864
1106	17	0.48875518861450473
1106	18	0.528551401290116
1106	19	0.5761317042342924
1106	20	0.47134934447883126
1106	21	0.5314173810020932
1106	22	0.48461396774714416
1106	23	0.3249099562324318
1106	24	0.3632584454745924
1106	25	0.5426451048393943
1107	1	0.3905280347202833
1107	2	0.39936580620354434
1107	3	0.41313140213203425
1107	4	0.5223505142351073
1107	5	0.3878825718497936
1107	6	0.4300754618220992
1107	7	0.45266456279739486
1107	8	0.4684305141892082
1107	9	0.562723687135751
1107	10	0.6053174161484165
1107	11	0.5402814626173154
1107	12	0.6208798476575268
1107	13	0.6117675768576945
1107	14	0.7162225984115693
1107	15	0.5030801600183636
1107	16	0.6221295480686564
1107	17	0.5933288628003908
1107	18	0.7153917492276136
1107	19	0.7475652674480813
1107	20	0.7782935814290184
1107	21	0.7374822225168245
1107	22	0.7766525591750479
1107	23	0.6398429307189417
1107	24	0.7023378164095035
1107	25	0.7150321679179257
1108	1	0.24046103317197165
1108	2	0.39620705370072073
1108	3	0.38105842184709005
1108	4	0.4358214525638644
1108	5	0.25561328214631374
1108	6	0.18648026243518956
1108	7	0.2311436031732299
1108	8	0.24505309319918922
1108	9	0.35922981563007994
1108	10	0.47216720406215273
1108	11	0.40067978693329565
1108	12	0.4327855176663391
1108	13	0.5253517346742669
1108	14	0.419072801107383
1108	15	0.4188607046913333
1108	16	0.5554793184031013
1108	17	0.41549451483827937
1108	18	0.4756995068542409
1108	19	0.4728372011648361
1108	20	0.5095503043924126
1108	21	0.4648648273928231
1108	22	0.4273003891571179
1108	23	0.4008487861780665
1108	24	0.5193209175998632
1108	25	0.507560749830489
1109	1	0.060619780207435525
1109	2	0.1342919314331681
1109	3	0.13255173884820692
1109	4	0.2328279646544189
1109	5	0.1203855171439443
1109	6	0.029639667621488286
1109	7	0.1320559636676606
1109	8	0.2618809292010056
1109	9	0.1328499176127096
1109	10	0.26595106170474025
1109	11	0.25060575594836365
1109	12	0.25256070950983656
1109	13	0.20480266641023093
1109	14	0.29023771050839287
1109	15	0.2678370560753365
1109	16	0.1981292498200915
1109	17	0.2244526594328704
1109	18	0.3588561177552876
1109	19	0.4365534072284305
1109	20	0.2698303304372658
1109	21	0.29321733262771943
1109	22	0.3115120624155452
1109	23	0.3135590056173285
1109	24	0.27155155684972954
1109	25	0.3476304487945825
1110	1	0.18159758474862642
1110	2	0.18153033961666054
1110	3	0.14982994140256167
1110	4	0.12086458005965994
1110	5	0.15377142808220867
1110	6	0.10522466293152632
1110	7	0.17302586420430544
1110	8	0.26122631371033833
1110	9	0.23140497112274971
1110	10	0.2501088459163867
1110	11	0.21167124076492883
1110	12	0.23958959906318
1110	13	0.22848029970956674
1110	14	0.252163571918139
1110	15	0.22249026093039959
1110	16	0.2805465555616422
1110	17	0.3255251175539797
1110	18	0.3285755499032632
1110	19	0.3139553859383213
1110	20	0.32933609400542707
1110	21	0.2898506642593972
1110	22	0.23591855884050972
1110	23	0.13792584310911593
1110	24	0.21652166158473563
1110	25	0.23103231707067096
1111	1	0.008464393830539763
1111	2	0.07865157492829766
1111	3	0.0723253534865762
1111	4	0.26215993240268176
1111	5	0.18874844682838443
1111	6	0.08098174273541758
1111	7	0.04157834577056008
1111	8	0.06876780532563487
1111	9	0.11496796545291232
1111	10	0.25481967881993484
1111	11	0.1800029601823141
1111	12	0.16950936125767413
1111	13	0.128708217980261
1111	14	0.16131045948502276
1111	15	0.2061320399523865
1111	16	0.20219426187305345
1111	17	0.27143039306382377
1111	18	0.2310807145184839
1111	19	0.25084175901540817
1111	20	0.20386014876171288
1111	21	0.18445033480838902
1111	22	0.14857287218872983
1111	23	0.16792464256444362
1111	24	0.1150000343533506
1111	25	0.13122016105913303
1112	1	-0.4064853316413952
1112	2	-0.35355774313130084
1112	3	-0.26435620311061214
1112	4	-1.1597580575282649
1112	5	-0.3449224134183666
1112	6	-0.33791643014544803
1112	7	-0.1099118151082692
1112	8	-0.11301647648001278
1112	9	-0.041831091719728875
1112	10	-9.481234792474424E-4
1112	11	0.017986546584552343
1112	12	0.03950144389121135
1112	13	-0.07258645534807329
1112	14	0.0062379274539736684
1112	15	-2.5126698091071376E-6
1112	16	0.007589072494546656
1112	17	0.014216260839312156
1112	18	0.05260842838016799
1112	19	0.012676074848926078
1112	20	-0.007497003023537729
1112	21	-0.05375845631444065
1112	22	0.052575844072173084
1112	23	-0.026759437537265285
1112	24	-0.10597013513354625
1112	25	0.034299246013290485
1113	1	-0.5480473944195374
1113	2	-0.4123908432796455
1113	3	-0.41177390988773155
1113	4	-1.2379522637375686
1113	5	-0.7847051890838657
1113	6	-0.6463757097223919
1113	7	-0.4560240482300202
1113	8	-0.44368086267651563
1113	9	-0.3949564951824627
1113	10	-0.235141935777186
1113	11	-0.36813947343510733
1113	12	-0.43994402375667363
1113	13	-0.48646544155985794
1113	14	-0.5099761693033074
1113	15	-0.37831222449531765
1113	16	-0.4714476627047972
1113	17	-0.429581380698707
1113	18	-0.4850600049592302
1113	19	-0.5166115318299092
1113	20	-0.44486591880554727
1113	21	-0.45357141428581116
1113	22	-0.491309867616323
1113	23	-0.5034007568636909
1113	24	-0.4521642135522086
1113	25	-0.4223380617408381
1114	1	-0.45862604476076996
1114	2	-0.46716226060237886
1114	3	-0.32541278812171015
1114	4	-1.133047876276862
1114	5	-0.6630818203931028
1114	6	-0.5322288453680741
1114	7	-0.562778276120234
1114	8	-0.4676877246508866
1114	9	-0.31858498645956956
1114	10	-0.3518428142061225
1114	11	-0.33015935360215565
1114	12	-0.40963069338561553
1114	13	-0.3702584341301076
1114	14	-0.5333400970035029
1114	15	-0.4734547872189907
1114	16	-0.4265215423882651
1114	17	-0.5246368316040417
1114	18	-0.5723869523079301
1114	19	-0.6320202911803889
1114	20	-0.634247882212577
1114	21	-0.6008466420582934
1114	22	-0.45619620924276205
1114	23	-0.49924945542420573
1114	24	-0.4667673507803016
1114	25	-0.48871196623653645
1115	1	-0.30952782182844274
1115	2	-0.14580739712550184
1115	3	-0.22770978884677007
1115	4	0.029222278549610792
1115	5	-0.14046226525368155
1115	6	-0.1792156643029159
1115	7	-0.21313084950526218
1115	8	-0.2755193202583186
1115	9	-0.19181655855621216
1115	10	-0.17560348207739196
1115	11	-0.1579300391890186
1115	12	-0.13192258537624468
1115	13	-0.16402841498266696
1115	14	-0.21622019465280573
1115	15	-0.22072217407775696
1115	16	-0.3129568654862567
1115	17	-0.3811354570480674
1115	18	-0.47302972773544383
1115	19	-0.5261955384487322
1115	20	-0.4246003289104152
1115	21	-0.4524768704458282
1115	22	-0.446662851180875
1115	23	-0.40269665795016607
1115	24	-0.33979953786958106
1115	25	-0.5251072060980988
1116	1	-0.12270560951254339
1116	2	-0.0645180344703995
1116	3	-0.10097999756259668
1116	4	0.021314623523338128
1116	5	-0.16396445086477485
1116	6	-0.1213307738581264
1116	7	-0.1258799130411532
1116	8	-0.22377030320231484
1116	9	-0.19459927775642427
1116	10	-0.12947457010818653
1116	11	-0.12920890161488785
1116	12	-0.1636554084167514
1116	13	-0.12157828050790442
1116	14	-0.2347786103646996
1116	15	-0.19184654369427534
1116	16	-0.24154738401166043
1116	17	-0.3354708157777324
1116	18	-0.36812065929803595
1116	19	-0.30392475295975174
1116	20	-0.3296672705126582
1116	21	-0.2529651205414325
1116	22	-0.3193161450203874
1116	23	-0.44348513643103615
1116	24	-0.33390345844813396
1116	25	-0.29575906539925256
1201	1	0.3659183059096556
1201	2	0.37723324082357124
1201	3	0.3237788139096551
1201	4	0.41409907957770997
1201	5	0.4175314574065254
1201	6	0.3429791803699942
1201	7	0.4819798277389751
1201	8	0.324096295654158
1201	9	0.3566241568298594
1201	10	0.5094333004976335
1201	11	0.4566444280197075
1201	12	0.5127649069006495
1201	13	0.4172862840427811
1201	14	0.5416212077806932
1201	15	0.5429100331238814
1201	16	0.5264163067122141
1201	17	0.5529770243135061
1201	18	0.524241524190181
1201	19	0.5278317622127773
1201	20	0.49394978683319124
1201	21	0.5903839324275779
1201	22	0.522334339668376
1201	23	0.599070306107528
1201	24	0.5755919720695388
1201	25	0.5180264386308266
1202	1	0.4348524859491292
1202	2	0.35907539878003547
1202	3	0.3599240125709464
1202	4	0.48876912162696584
1202	5	0.43592300178708854
1202	6	0.4073257005793849
1202	7	0.3294156811672764
1202	8	0.4015387282180214
1202	9	0.32588757969664073
1202	10	0.3911638991989719
1202	11	0.3423611247215632
1202	12	0.49404812438398693
1202	13	0.4193670071755946
1202	14	0.47726982473197666
1202	15	0.5215049586080198
1202	16	0.5118987289783021
1202	17	0.5139275663770633
1202	18	0.47777108739278873
1202	19	0.577506524076675
1202	20	0.5251283102463304
1202	21	0.48370954242786013
1202	22	0.5234113155544122
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2210	17	-0.15724241002858008
2210	18	-0.1851233062570259
2210	19	-0.17966593000080877
2210	20	-0.15190743069931756
2210	21	-0.1623588300205654
2210	22	-0.16446512203349073
2210	23	-0.17154439921873177
2210	24	-0.19464503481489714
2210	25	-0.23184955260514073
2211	1	-0.695787120446937
2211	2	-0.613127392813098
2211	3	-0.405921219794422
2211	4	-1.2321159524411627
2211	5	-0.7796441408408654
2211	6	-0.6834582985649575
2211	7	-0.5793211496792949
2211	8	-0.5379259490374437
2211	9	-0.5789201640329651
2211	10	-0.5696297261285892
2211	11	-0.5163249012691367
2211	12	-0.5210200610804776
2211	13	-0.5004757651651772
2211	14	-0.5735254049594474
2211	15	-0.5173056729765548
2211	16	-0.5741903666952695
2211	17	-0.6072858674964081
2211	18	-0.6155697329719345
2211	19	-0.6419693837258791
2211	20	-0.7351503142790463
2211	21	-0.6548941262175205
2211	22	-0.6196407193137574
2211	23	-0.6066189422915258
2211	24	-0.5545664370979679
2211	25	-0.628134666862934
2212	1	-0.20209546556716163
2212	2	-0.34778413799072183
2212	3	-0.28869226708548723
2212	4	-0.005447645858950523
2212	5	-0.17650821234118297
2212	6	-0.28725798288121496
2212	7	-0.30512235091742923
2212	8	-0.36620424270532226
2212	9	-0.32445036922739945
2212	10	-0.18382196522827599
2212	11	-0.2849688961034005
2212	12	-0.3257364581860287
2212	13	-0.39244058897674705
2212	14	-0.4679017245789865
2212	15	-0.3233048940166725
2212	16	-0.40541312872301916
2212	17	-0.33499552044927583
2212	18	-0.3395625173435377
2212	19	-0.35402563282904964
2212	20	-0.3725629375289472
2212	21	-0.4017014156421155
2212	22	-0.3893768979005614
2212	23	-0.4293309526229905
2212	24	-0.5087574462610007
2212	25	-0.5083564654403077
2213	1	-0.1325170956740216
2213	2	-0.15552833107228992
2213	3	-0.20079502153929596
2213	4	-0.013632246317349939
2213	5	-0.21347245376945523
2213	6	-0.17869974472874617
2213	7	-0.3430148272005695
2213	8	-0.4050342198401111
2213	9	-0.5070308403215762
2213	10	-0.4491330710704844
2213	11	-0.4533260621255337
2213	12	-0.4647225946196514
2213	13	-0.5712372630289622
2213	14	-0.5543758220300248
2213	15	-0.545381403897224
2213	16	-0.49853149093083715
2213	17	-0.5147809308648021
2213	18	-0.49198543923936455
2213	19	-0.5096714864317562
2213	20	-0.40015707596839434
2213	21	-0.44289422195825523
2213	22	-0.5873296576686968
2213	23	-0.5764591749415118
2213	24	-0.6539201871428517
2213	25	-0.6873787937672802
2214	1	-0.3699703224039901
2214	2	-0.38099799407131485
2214	3	-0.4139713752005463
2214	4	-0.19150583075560945
2214	5	-0.39046363731713996
2214	6	-0.41887521636319036
2214	7	-0.506462346680685
2214	8	-0.5975447805567242
2214	9	-0.7106115893178782
2214	10	-0.7155160137540051
2214	11	-0.6970384694646512
2214	12	-0.6226141457994672
2214	13	-0.7754190980651856
2214	14	-0.7027678345736703
2214	15	-0.6609459186324713
2214	16	-0.8645803198567705
2214	17	-0.7141943295469773
2214	18	-0.7944675115900992
2214	19	-0.8898922991368892
2214	20	-0.8949318396620498
2214	21	-0.8245428571659872
2214	22	-0.8908812501763492
2214	23	-0.8689591339787768
2214	24	-0.9632514531113046
2214	25	-0.9707877192440577
2215	1	-0.5973871204469354
2215	2	-0.5902520304942556
2215	3	-0.6808661473306543
2215	4	-0.4311536336005872
2215	5	-0.7181832712756417
2215	6	-0.6635510521881471
2215	7	-0.7508573815633568
2215	8	-0.7357114562838234
2215	9	-0.8904332075112293
2215	10	-0.9494442188822134
2215	11	-0.8153741766314511
2215	12	-0.8753157132543947
2215	13	-0.8060873593680711
2215	14	-1.0434268542348093
2215	15	-1.0896419048606134
2215	16	-0.9674193522025121
2215	17	-0.9302539834384334
2215	18	-1.1335755300733794
2215	19	-0.9954302532910972
2215	20	-0.9079850968877423
2215	21	-0.9876129667972293
2215	22	-1.0859943425021683
2215	23	-1.066387058233552
2215	24	-1.0237258573878236
2215	25	-1.1176882900513405
2216	1	-0.6702455587340879
2216	2	-0.6571517786050869
2216	3	-0.6475858786488757
2216	4	-0.45522550597674183
2216	5	-0.7601909958768189
2216	6	-0.7252558380823544
2216	7	-0.9638011263156656
2216	8	-0.8538132195080053
2216	9	-1.0721447104499333
2216	10	-1.1270254111576108
2216	11	-1.0962964268413629
2216	12	-1.1764495503660655
2216	13	-1.208639164573782
2216	14	-1.4474698433196167
2216	15	-1.3725665060360939
2216	16	-1.442315070086643
2216	17	-1.442775226091669
2216	18	-1.3897689809549973
2216	19	-1.4306248796554968
2216	20	-1.2788512597760686
2216	21	-1.4204035629349292
2216	22	-1.3834102954828538
2216	23	-1.4819907526080272
2216	24	-1.4827451688907658
2216	25	-1.6990746880362266
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54265.0
3	1.0
4	912.0
5	7560.0
6	8472.0
7	6368.0
8	5197.0
9	4563.0
10	4070.0
11	3865.0
12	3352.0
13	3200.0
14	3174.0
15	3236.0
16	3175.0
17	3365.0
18	3766.0
19	4506.0
20	5684.0
21	7592.0
22	9476.0
23	11387.0
24	13405.0
25	14825.0
26	16390.0
27	18180.0
28	20435.0
29	24759.0
30	31125.0
31	39524.0
32	50872.0
33	66654.0
34	88146.0
35	119143.0
36	171801.0
37	279583.0
38	1083685.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.616423781597796	19.421254960430616	13.396196630602697	27.566124627368893
2	13.699026335890204	20.82148155267019	39.832185617901175	25.647306493538437
3	16.764948216015664	25.09584102118682	30.209335828669005	27.929874934128513
4	11.208778396320382	17.625974477868418	37.82169668113914	33.34355044467207
5	12.612042242061838	38.252138744816946	36.103343456733064	13.032475556388157
6	31.447261394481664	37.715757149254685	18.15243517917691	12.684546277086742
7	27.31785786526375	31.73294020529885	22.840544426444296	18.108657502993104
8	24.862849594606907	36.701238954176915	20.833196683975586	17.60271476724059
9	26.23570542378625	14.827918534807077	20.03094387471602	38.90543216669066
10	14.278915040931453	29.120540785253564	35.12631044870201	21.474233725112978
11	33.942869582682256	22.44910878607541	24.817542183336347	18.790479447905987
12	22.6827914212832	25.047535811829686	31.926531381833602	20.343141385053514
13	28.61416769860178	21.842198866609618	25.960086769081386	23.58354666570722
14	20.838244483268085	21.82882418417222	27.857879366976306	29.475051965583386
15	22.994125370665476	30.384253315437853	23.48840672710869	23.133214586787982
16	22.367677378600938	28.098526537848983	27.27765422894522	22.25614185460486
17	21.765081829284348	28.409044838357154	27.646258429028975	22.17961490332952
18	21.721804764755355	27.58417409479762	29.07658833530597	21.61743280514106
19	23.19733954300949	27.163477193968426	27.59445337346001	22.04472988956207
20	23.480026761238832	27.22491509591645	27.780907614064315	21.5141505287804
21	23.044523983999586	26.740805750166345	27.240511310096633	22.974158955737437
22	23.035864009425648	27.75875331498544	27.096735408257278	22.10864726733163
23	22.308612331230336	27.437652464296708	27.473542491423743	22.780192713049214
24	22.796558566625055	27.172403679351536	27.878142544130313	22.152895209893096
25	22.687093155702183	27.11937044538193	27.544244210645207	22.649292188270678
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	214.0
1	214.0
2	313.0
3	412.0
4	412.0
5	412.0
6	980.0
7	1548.0
8	1548.0
9	1548.0
10	2372.5
11	3197.0
12	3197.0
13	3197.0
14	6045.0
15	8893.0
16	8893.0
17	8893.0
18	17009.5
19	25126.0
20	25126.0
21	25126.0
22	40168.0
23	55210.0
24	55210.0
25	55210.0
26	78912.5
27	102615.0
28	102615.0
29	102615.0
30	131665.0
31	160715.0
32	160715.0
33	160715.0
34	185264.5
35	209814.0
36	209814.0
37	209814.0
38	229113.0
39	248412.0
40	248412.0
41	248412.0
42	261452.5
43	274493.0
44	274493.0
45	274493.0
46	283858.5
47	293224.0
48	293224.0
49	293224.0
50	280874.0
51	268524.0
52	268524.0
53	268524.0
54	238917.5
55	209311.0
56	209311.0
57	209311.0
58	179662.5
59	150014.0
60	150014.0
61	150014.0
62	122403.5
63	94793.0
64	94793.0
65	94793.0
66	72701.5
67	50610.0
68	50610.0
69	50610.0
70	37080.5
71	23551.0
72	23551.0
73	23551.0
74	16507.0
75	9463.0
76	9463.0
77	9463.0
78	6558.0
79	3653.0
80	3653.0
81	3653.0
82	2501.5
83	1350.0
84	1350.0
85	1350.0
86	894.5
87	439.0
88	439.0
89	439.0
90	281.0
91	123.0
92	123.0
93	123.0
94	66.0
95	9.0
96	9.0
97	9.0
98	4.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17611591314529723
2	0.008744312212024067
3	0.006831493915643802
4	0.002277164638547934
5	0.0813403208889322
6	0.33579069760027835
7	0.03046846286377136
8	0.014255050637310068
9	0.005692911596369835
10	0.00414443964215724
11	0.0
12	0.0
13	0.0
14	0.0013207554903578017
15	0.0
16	0.0
17	0.0214964341878925
18	0.030923895791480947
19	0.0
20	0.0
21	9.108658554191736E-4
22	9.108658554191736E-5
23	0.005328565254202166
24	0.0
25	9.108658554191736E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2195713.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.5245898782089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.552935578361	59.871177960628316
2	11.990732036382848	17.392458465563333
3	2.9888377636888297	6.502926990721061
4	1.0519035165706139	3.05155484522918
5	0.48736611857161827	1.7673013934970572
6	0.25013603881766006	1.088460817740632
7	0.16297930772602082	0.8274005216024845
8	0.09873310271875414	0.5728462225664573
9	0.06858856408708813	0.4476921732676182
>10	0.316459683180136	3.8924823619517253
>50	0.017152198077146637	0.8543453271258156
>100	0.01184714802167065	1.7614103795824576
>500	0.001510666246401256	0.7724468224273412
>1k	8.182775501338813E-4	1.197495718096537
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3268	0.14883548077549297	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2651	0.12073526913581147	No Hit
CCCATGTACTCTGCGTTGATACCAC	2524	0.1149512709538997	No Hit
GTCCTACAGTGGACATTTCTAAATT	2274	0.10356544776116004	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2231	0.10160708617200882	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.554329277095868E-5	0.0	0.0	0.0	1.3662987831287605E-4
2	4.554329277095868E-5	0.0	0.0	0.0	1.3662987831287605E-4
3	4.554329277095868E-5	0.0	0.0	0.0	1.8217317108383473E-4
4	4.554329277095868E-5	0.0	0.0	0.0	1.8217317108383473E-4
5	4.554329277095868E-5	4.554329277095868E-5	0.0	0.0	1.8217317108383473E-4
6	4.554329277095868E-5	4.554329277095868E-5	0.0	0.0	1.8217317108383473E-4
7	4.554329277095868E-5	4.554329277095868E-5	0.0	4.554329277095868E-5	1.8217317108383473E-4
8	4.554329277095868E-5	4.554329277095868E-5	0.0	4.554329277095868E-5	1.8217317108383473E-4
9	4.554329277095868E-5	4.554329277095868E-5	0.0	9.108658554191736E-5	1.8217317108383473E-4
10	4.554329277095868E-5	4.554329277095868E-5	0.0	9.108658554191736E-5	1.8217317108383473E-4
11	4.554329277095868E-5	4.554329277095868E-5	0.0	9.108658554191736E-5	1.8217317108383473E-4
12	4.554329277095868E-5	4.554329277095868E-5	0.0	9.108658554191736E-5	4.0988963493862813E-4
13	4.554329277095868E-5	4.554329277095868E-5	0.0	1.3662987831287605E-4	5.009762204805455E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGTGC	80	2.9036413E-5	11.860523	8
TGGCGAG	1290	0.0	11.40315	18
GGACGTG	1140	0.0	11.190776	6
TAGGACC	580	0.0	10.9988365	4
AGGACGT	1195	0.0	10.915726	5
GGCGAGG	565	0.0	10.914637	19
TATGGCG	1305	0.0	10.763508	16
TCCAACG	160	5.5479177E-10	10.67666	18
GACGTGG	555	0.0	10.602331	7
GGCGAGA	845	0.0	10.553962	19
ATGGCGA	1350	0.0	10.545329	17
TAGGACG	1220	0.0	10.535954	4
TATTAGG	210	0.0	10.429848	2
GACGTGA	675	0.0	10.404725	7
GTAGGAC	1370	0.0	10.147569	3
CGTGAAA	730	0.0	10.1371565	9
ACTTCGC	75	0.0026752483	10.120979	8
GTCCTAT	295	0.0	10.010781	1
ATATGGC	2375	0.0	9.910389	15
CCAACGA	115	1.04315895E-5	9.899831	19
>>END_MODULE