FastQCFastQC Report
Thu 26 May 2016
SRR1513134_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513134_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580874
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT21130.13366024110713443No Hit
GTCCTACAGTGGACATTTCTAAATT20320.12853649310444729No Hit
GTATCAACGCAGAGTACTTTTTTTT19890.12581647873265042No Hit
CTGTAGGACGTGGAATATGGCAAGA17780.11246943146639138No Hit
CTTTAGGACGTGAAATATGGCGAGG16890.10683963427825369No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC456.824718E-414.75991258
TAGGACC5200.011.1770834
AGCACCG600.005820822411.0973215
GTGTAGG2200.010.8521021
GGCGAGG4700.010.4992619
GTCCTAA7200.010.2130351
AGCCCGT750.002676419410.1201226
AAGGCGT951.6656429E-49.9869626
AAAGGCG1151.0109034E-59.9255535
TGGCGAG10100.09.86554618
CCTATAC1553.8819053E-89.8350483
CTACACT2550.09.7148294
TTTAGAA4950.09.6300612
GTCCTAT2106.002665E-119.549851
GTCTAGG1409.851883E-79.549851
TCAATAC1803.974492E-99.5277033
GTAAGAC1803.974492E-99.5277033
GGTATCA9150.09.4976651
GCGCCAC800.0045572289.48941613
CGCAGAT900.00112592189.4885158
Produced by FastQC (version 0.11.5)