FastQCFastQC Report
Thu 26 May 2016
SRR1513134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513134_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580874
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29930.1893256515066982No Hit
GTCCTAAAGTGTGTATTTCTCATTT23030.1456789092615857No Hit
CTTTAGGACGTGAAATATGGCGAGG21980.1390370137025468No Hit
GTCCTACAGTGGACATTTCTAAATT21870.13834119607255227No Hit
CTGTAGGACGTGGAATATGGCAAGA21150.13378675340349705No Hit
TATCAACGCAGAGTACTTTTTTTTT20820.13169930051351342No Hit
GGTATCAACGCAGAGTACTTTTTTT18090.11443037206001237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTC701.10152556E-412.20163611
TAGGACC4750.011.8274414
GCGTCAG658.093594E-411.68019913
GGCGAGG6650.011.41420719
TTAGGAC9150.011.0310583
TGGCGAG13650.010.77603318
CTAGACC1158.555144E-710.7641054
TTTAGGA10150.010.6947642
GTCGAGG803.8148218E-410.674067519
GGACGTG12600.010.5787596
GTATAAG1909.094947E-1210.52776051
AGGACGT13150.010.4996455
GACGTGG5900.010.4562557
GTCCTAA7550.010.2189991
GTATTAG1409.3174094E-810.2054811
TAGGACG14250.010.1568994
AGGGCGT750.002674674810.12091810
ATAGGAC2450.010.1050783
AAAGGCG951.6057715E-410.0232555
TATGTCG951.6611381E-49.98964416
Produced by FastQC (version 0.11.5)