FastQCFastQC Report
Thu 26 May 2016
SRR1513133_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513133_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139335
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21680.10133990235283394No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG551.8982122E-413.8710322
TAGGACC5500.013.5150444
GAGCCGT550.003095702512.0769811
TGCGTGA550.003095702512.0769810
GGCGAGG6000.011.86159719
TGGCGAG12750.011.38713418
GGACCGT752.1009389E-411.3844716
AGGCGTG1701.2732926E-1111.1617687
GTCCTAC10850.011.0934861
TTAGGAC9350.010.9058063
CGCAGAT1158.971292E-710.7250038
GTATTAG2050.010.717691
GTATTAC1553.3451215E-910.47718051
AGGACGT10500.010.4161615
TAGGACT4000.010.2445764
GGACGTG10500.010.2098836
ATTTAGA5900.010.2003661
TTTAGAA7150.010.1365232
ATGGCGA15050.010.08779617
TGGACCG951.6264086E-410.0109545
Produced by FastQC (version 0.11.5)