##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513133_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2139335
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.42950309325094	31.0	30.0	34.0	25.0	34.0
2	29.256503072216365	31.0	30.0	34.0	22.0	34.0
3	29.468394150518737	31.0	30.0	34.0	23.0	34.0
4	32.53864027840427	37.0	33.0	37.0	22.0	37.0
5	32.70891608841065	37.0	33.0	37.0	25.0	37.0
6	33.056707808734956	37.0	35.0	37.0	26.0	37.0
7	33.02041195044254	37.0	35.0	37.0	26.0	37.0
8	32.898827906802815	37.0	35.0	37.0	26.0	37.0
9	34.13644473633162	39.0	35.0	39.0	25.0	39.0
10	33.724305450058075	39.0	34.0	39.0	19.0	39.0
11	34.17782021048597	39.0	35.0	39.0	25.0	39.0
12	34.16478438393239	39.0	35.0	39.0	25.0	39.0
13	34.20328793760678	39.0	35.0	39.0	25.0	39.0
14	34.42925161323495	39.0	34.0	41.0	17.0	41.0
15	34.86069970341251	40.0	34.0	41.0	21.0	41.0
16	34.708777260223385	39.0	34.0	41.0	18.0	41.0
17	34.32257827782932	39.0	34.0	41.0	17.0	41.0
18	34.05754451733833	39.0	33.0	41.0	16.0	41.0
19	34.68927493824015	39.0	34.0	41.0	18.0	41.0
20	34.90340222545791	40.0	35.0	41.0	22.0	41.0
21	34.94758417919587	40.0	35.0	41.0	22.0	41.0
22	34.6306828991252	40.0	35.0	41.0	18.0	41.0
23	34.17339360128264	39.0	34.0	41.0	10.0	41.0
24	34.4281615548757	39.0	34.0	41.0	16.0	41.0
25	34.06938277548864	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.057173223186524
1101	2	1.1851527379591111
1101	3	1.1594806290824877
1101	4	1.6087211438476174
1101	5	1.4105565834327436
1101	6	1.1693777352945816
1101	7	1.0671154976114678
1101	8	1.1046843134532764
1101	9	1.573655445809905
1101	10	1.9686224521221192
1101	11	1.4419305693609559
1101	12	1.3702584542658087
1101	13	1.2815579270506916
1101	14	2.3019532344708793
1101	15	1.8073960824956643
1101	16	2.022491312712077
1101	17	2.3373370172624632
1101	18	2.3893018294291366
1101	19	1.8785098076232387
1101	20	1.552992804323651
1101	21	1.5029749160282222
1101	22	1.7750251964353083
1101	23	2.050887627047196
1101	24	1.743111312278259
1101	25	2.078328087862701
1102	1	0.8592201234430128
1102	2	1.1572545184996557
1102	3	1.0038764046688442
1102	4	1.2538399904115494
1102	5	1.293351480868317
1102	6	1.018148429765219
1102	7	0.9090104767889216
1102	8	1.0330882396301533
1102	9	1.381165845699293
1102	10	1.6132513442019345
1102	11	1.241291310200289
1102	12	1.1968928105697358
1102	13	1.135778666552845
1102	14	2.078427801858872
1102	15	1.6658702354819326
1102	16	1.6682722291608698
1102	17	2.013439326879336
1102	18	2.3196010949527945
1102	19	1.6629587102556016
1102	20	1.5623426138168455
1102	21	1.3562518669919967
1102	22	1.57641385619349
1102	23	1.8131844599789275
1102	24	1.5649031505514657
1102	25	1.9593663318426522
1103	1	0.7808215607558182
1103	2	0.9699236838855043
1103	3	0.8158411948992708
1103	4	1.2490345998893062
1103	5	1.0621142952929787
1103	6	0.8953730338600394
1103	7	0.8670287987322141
1103	8	0.8420141199492974
1103	9	1.1939912060012148
1103	10	1.3808713591223452
1103	11	1.1364410785544408
1103	12	1.0441194020551094
1103	13	1.0672175872499707
1103	14	1.9326248411462927
1103	15	1.5613087247455297
1103	16	1.6682139057395204
1103	17	2.0518070500588053
1103	18	2.289185131113314
1103	19	1.600243155778628
1103	20	1.221775877941056
1103	21	1.3654072459793838
1103	22	1.5955893787323916
1103	23	2.0221987242499395
1103	24	1.7177377786847288
1103	25	1.9338589230703676
1104	1	0.7740091003816651
1104	2	1.0216656531294817
1104	3	0.9346002531099415
1104	4	1.3429045614663266
1104	5	1.2380111990140605
1104	6	1.0591112832968292
1104	7	1.012904624199173
1104	8	1.0244762481768106
1104	9	1.3528423578577389
1104	10	1.4973873618387046
1104	11	1.1500095554423453
1104	12	0.9565206865449056
1104	13	0.9796449378280769
1104	14	2.052044174932348
1104	15	1.5958955821454026
1104	16	1.5932022690929486
1104	17	2.007167795901701
1104	18	2.0357094730180805
1104	19	1.4184399312271196
1104	20	1.2421744539094917
1104	21	1.2693988852325617
1104	22	1.5681193473844957
1104	23	2.01256975443858
1104	24	1.7109064056976209
1104	25	1.9501545399498283
1105	1	0.504296575502515
1105	2	0.8736312905229191
1105	3	0.6569069122675053
1105	4	1.0300749022715578
1105	5	0.8399847595469438
1105	6	0.6375043013279225
1105	7	0.5253071425173701
1105	8	0.6565304751274113
1105	9	0.8765401234536725
1105	10	1.108041927328813
1105	11	0.6428000890918639
1105	12	0.7426217086397386
1105	13	0.6654890139870631
1105	14	1.3067068686777148
1105	15	0.9984624234229713
1105	16	1.3283143948033498
1105	17	1.4664285876605376
1105	18	1.0457341636859212
1105	19	0.935196632972783
1105	20	0.8125154953584328
1105	21	0.8158599780903799
1105	22	0.9661175391370662
1105	23	1.2408189650281471
1105	24	1.0112078590080884
1105	25	1.2254888521435632
1106	1	0.46239258400915517
1106	2	0.6145971780715591
1106	3	0.6102428635227284
1106	4	1.0306252154946876
1106	5	0.9080344689173714
1106	6	0.5841523814689182
1106	7	0.5531296128652841
1106	8	0.6686540511171088
1106	9	0.7859472995891394
1106	10	0.6477408872495829
1106	11	0.6988195607774301
1106	12	0.6911135883544404
1106	13	0.5569635560380348
1106	14	1.3530423194786607
1106	15	0.8807706728311686
1106	16	1.1220688353658446
1106	17	1.37919456870452
1106	18	1.603830780707277
1106	19	1.0799296976894013
1106	20	0.8963858020292363
1106	21	0.7432422861925687
1106	22	0.8350256075808389
1106	23	1.3928011084584
1106	24	0.9258411950339394
1106	25	1.2546876453607467
1107	1	0.4743164757616185
1107	2	0.6616906924258821
1107	3	0.6166422054313969
1107	4	0.8994517030592917
1107	5	0.7931644468296852
1107	6	0.5924781007474422
1107	7	0.5640683362326939
1107	8	0.6123077519586744
1107	9	0.8925887908984151
1107	10	1.1182597543852566
1107	11	0.7381198379796743
1107	12	0.7282030899800276
1107	13	0.5765249157634429
1107	14	1.40993572204232
1107	15	0.8502984944011089
1107	16	1.0692778960480709
1107	17	1.593159973229909
1107	18	1.746574216159253
1107	19	1.180568819587009
1107	20	0.9028227961198567
1107	21	0.8012354382736504
1107	22	1.201107642893426
1107	23	1.5019522992659944
1107	24	1.0936169286996034
1107	25	1.4461116250797943
1108	1	0.5893398037337612
1108	2	0.8477305812923568
1108	3	0.6287610107129211
1108	4	1.1273569147307327
1108	5	0.9944026096797671
1108	6	0.6963166977105573
1108	7	0.6407226894340283
1108	8	0.7284447485387631
1108	9	0.910146870144807
1108	10	1.1798391152388703
1108	11	0.9055686356805737
1108	12	0.6567456732208541
1108	13	0.6710185442524903
1108	14	1.6738393551208972
1108	15	1.2379450130636727
1108	16	1.3556214509172122
1108	17	1.695311118352791
1108	18	1.8445187199479136
1108	19	1.2218192040383045
1108	20	1.0588202731639313
1108	21	1.057828158006167
1108	22	1.1998154823193659
1108	23	1.4100814829872377
1108	24	1.186602883412064
1108	25	1.5873392823502286
1109	1	0.5074930668688431
1109	2	0.5724966084717558
1109	3	0.5945602306753806
1109	4	0.8037966580571592
1109	5	0.8055007909467875
1109	6	0.3655415697270712
1109	7	0.4071628428055334
1109	8	0.3928347497359468
1109	9	0.5056323653492925
1109	10	0.8850563347890912
1109	11	0.5166276979147995
1109	12	0.5029132344814045
1109	13	0.2551991515365728
1109	14	1.3249000538743232
1109	15	0.8835058906626543
1109	16	1.1324780306945996
1109	17	1.3612103234145039
1109	18	1.5241668829642592
1109	19	0.857052528841237
1109	20	0.6783613192255373
1109	21	0.5976912212552534
1109	22	0.8594196716620743
1109	23	1.2400743218428119
1109	24	0.9345203486598308
1109	25	1.127805606116695
1110	1	0.43070728597845687
1110	2	0.6859899857735741
1110	3	0.5347809726545307
1110	4	0.9196842773304539
1110	5	0.7225237142367007
1110	6	0.43968490830415163
1110	7	0.3653017296832246
1110	8	0.42548255646768496
1110	9	0.6210740467708504
1110	10	0.9394900979341116
1110	11	0.5906923254442518
1110	12	0.48489635795517927
1110	13	0.4370296249437544
1110	14	1.1868290833863
1110	15	0.9173250384551253
1110	16	1.0632350719051757
1110	17	1.3376184009780658
1110	18	0.9818639458080369
1110	19	0.8217088001645294
1110	20	0.5777170405039627
1110	21	0.45316183104372953
1110	22	0.6190421657985183
1110	23	-0.47047361675050325
1110	24	0.21999681652487624
1110	25	0.7342797131988306
1111	1	0.18065676658254404
1111	2	0.5113184678317673
1111	3	0.4569814562276022
1111	4	0.8417538697413818
1111	5	0.6881816534127552
1111	6	0.331137980378017
1111	7	0.37217024783276287
1111	8	0.350846331446526
1111	9	0.5885860124487508
1111	10	0.8180338745804434
1111	11	0.36855500878019853
1111	12	0.28727472632706963
1111	13	0.07773656440102883
1111	14	1.0169957691302187
1111	15	0.5947164782791035
1111	16	0.6996948492806823
1111	17	1.0582825296633374
1111	18	1.3848389729754587
1111	19	0.8227594622865695
1111	20	0.46265470027596933
1111	21	0.2833477706832994
1111	22	0.6692831751124544
1111	23	0.9810175179787208
1111	24	0.6494000234820021
1111	25	0.8953816507413066
1112	1	-0.03855360466082658
1112	2	0.2037168178612312
1112	3	0.09015764950986593
1112	4	0.4775818013854547
1112	5	0.12778094628252745
1112	6	0.09542790377224009
1112	7	0.06633642343676627
1112	8	0.0037632436445207418
1112	9	0.2453332133915893
1112	10	0.4311747113512112
1112	11	-0.033919947024166675
1112	12	-0.061576187049475095
1112	13	-0.10258164420238813
1112	14	0.36437349452798173
1112	15	0.13655501687662763
1112	16	0.1602134114492202
1112	17	0.6457898956032295
1112	18	0.6623875881787598
1112	19	0.15157501915162186
1112	20	0.007734252426821797
1112	21	-0.12118964829136303
1112	22	0.2729956088189951
1112	23	0.6138990736652232
1112	24	0.26754969938064477
1112	25	0.6142973843889266
1113	1	-0.0018959805207678926
1113	2	0.13595557543202474
1113	3	0.16952761321190835
1113	4	0.40766726372240214
1113	5	0.44296594033747283
1113	6	0.1003155753681284
1113	7	-0.010357742738840159
1113	8	0.05945585120467456
1113	9	0.1518145431311595
1113	10	0.4039066120600907
1113	11	0.07298625361531919
1113	12	-0.09783177906183482
1113	13	-0.20591767402180494
1113	14	0.4915922550974514
1113	15	0.24147790551059245
1113	16	0.3441387513285008
1113	17	0.5908274313868773
1113	18	0.640437087294849
1113	19	0.21642876383855736
1113	20	0.05363547735211682
1113	21	0.08652567251493082
1113	22	0.2325026400226875
1113	23	0.44872593485082035
1113	24	0.3045987208002785
1113	25	0.6513390834541042
1114	1	-0.8685036363197902
1114	2	-0.5055093176940133
1114	3	-0.555596550705129
1114	4	-0.4109707562678153
1114	5	-3.3477649907713563
1114	6	-1.3203348989001462
1114	7	-1.0079712468133408
1114	8	-0.6731060929852788
1114	9	-0.4591838656419398
1114	10	-0.2857931213577416
1114	11	-0.6471413889579196
1114	12	-0.8230337822167328
1114	13	-0.7941336400205259
1114	14	-5.987488621937217
1114	15	-2.4169717848545673
1114	16	-1.3502647452376166
1114	17	-0.39078769835666805
1114	18	-6.778363844020635
1114	19	-2.5759037268104024
1114	20	-1.4781325754959056
1114	21	-1.0947732783774669
1114	22	-0.7140245589357832
1114	23	-0.45540953297677333
1114	24	-0.5437649330975418
1114	25	-0.3410585028406814
1115	1	-0.864531515915882
1115	2	-0.5055677880695164
1115	3	-0.4969173759285397
1115	4	-3.20767805998252
1115	5	-1.1236049493766345
1115	6	-0.7864888574021336
1115	7	-0.7890946740753719
1115	8	-0.5150666712877197
1115	9	-0.22575425492799894
1115	10	-0.6158301838455529
1115	11	-0.6960466360285622
1115	12	-0.7120653154300456
1115	13	-0.7337932815798496
1115	14	-6.328274036152543
1115	15	-2.6525441580215485
1115	16	-1.3151187864040494
1115	17	-0.4794855310218509
1115	18	-0.3336375100490301
1115	19	-0.5510007744758454
1115	20	-0.5828722614367976
1115	21	-0.7422948581401059
1115	22	-1.971996842497532
1115	23	-1.1251039672878207
1115	24	-0.9022465585970068
1115	25	-0.5387550660983891
1116	1	-0.15978578605537308
1116	2	0.06612648104515984
1116	3	-0.03383645928690626
1116	4	0.24414604825536657
1116	5	-0.487681603769083
1116	6	-0.18551550954816776
1116	7	-0.07008179004501613
1116	8	-0.031642361206586145
1116	9	-0.09117389469201242
1116	10	0.21167589725785518
1116	11	-0.049105901677073405
1116	12	-0.4609840467880417
1116	13	-0.3581974522643847
1116	14	0.5259323868145174
1116	15	0.07327777954740355
1116	16	0.1302828003452916
1116	17	0.5444337108852011
1116	18	0.39188255378631
1116	19	0.267390205106274
1116	20	-0.40801689534342245
1116	21	-0.33789641303118145
1116	22	-0.3326732030365065
1116	23	-1.0720175589948653
1116	24	-0.5876926429456546
1116	25	0.08434454963454385
1201	1	0.8432682716726632
1201	2	1.0161052665896726
1201	3	0.9539547245620952
1201	4	1.3531422131761346
1201	5	1.1862752835141919
1201	6	0.9389153045514149
1201	7	0.8681689030291864
1201	8	1.0087602603234131
1201	9	1.2252930540102014
1201	10	1.6635549213105207
1201	11	1.0890080302471787
1201	12	1.0541659579491665
1201	13	0.950435389360436
1201	14	1.9398746093479744
1201	15	1.6266228666502442
1201	16	1.6030302577001194
1201	17	1.9509014422566437
1201	18	1.310499783219612
1201	19	1.2559179218953531
1201	20	1.3336145168802247
1201	21	1.1969892142782044
1201	22	1.4273459929562264
1201	23	1.7150593361974842
1201	24	1.3894941966771839
1201	25	1.713558296601697
1202	1	0.982350531357099
1202	2	1.0549621115493082
1202	3	1.0415326649430448
1202	4	1.4290036930787764
1202	5	1.2408269232197782
1202	6	1.0228268194542167
1202	7	0.962504769005605
1202	8	1.1303223176058452
1202	9	1.3877717127533202
1202	10	1.6546904087158651
1202	11	1.2061797037846063
1202	12	1.0846516304675191
1202	13	1.1882905329101519
1202	14	1.9808085881633133
1202	15	1.612339368362946
1202	16	1.6919613736699048
1202	17	2.045181445437585
1202	18	2.231182467814733
1202	19	1.7326080169013807
1202	20	1.5059306237244527
1202	21	1.4432959373509178
1202	22	1.470478648932712
1202	23	1.8839244182195785
1202	24	1.6132470769491718
1202	25	1.9670867341026153
1203	1	0.7383838422045983
1203	2	0.8980814116026252
1203	3	0.7650976871256034
1203	4	1.2326233422508537
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2211	15	-0.013211925930889379
2211	16	-6.375189988226197
2211	17	-1.7479204069824448
2211	18	-0.552877300979155
2211	19	-0.41065547293987237
2211	20	-0.5089006530767435
2211	21	-0.46908146914113047
2211	22	-0.5562462603555645
2211	23	-0.013778069807855786
2211	24	-0.4825996465379916
2211	25	-7.061993199670397
2212	1	-1.694947546782533
2212	2	-4.152719035458762
2212	3	-1.9571313017419492
2212	4	-3.946730964717897
2212	5	-1.6001272322887843
2212	6	-1.3401544313128078
2212	7	-1.2134042342202136
2212	8	-1.099690201658472
2212	9	-1.2473357301722459
2212	10	-0.673060548813865
2212	11	-1.2506532524393847
2212	12	-1.2692505988080285
2212	13	-1.5944955736094997
2212	14	-6.8302958536839675
2212	15	-5.425429143991586
2212	16	-3.230613447315829
2212	17	-7.46772963938837
2212	18	-3.197636837048208
2212	19	-2.142723696575594
2212	20	-2.216577526729921
2212	21	-1.8588524327978675
2212	22	-1.7396323730572618
2212	23	-1.2274919697262234
2212	24	-1.4521725227943065
2212	25	-1.3545955048479286
2213	1	-0.3265802325650853
2213	2	-0.3832780653864134
2213	3	-0.2525176497663999
2213	4	-0.017526188382049668
2213	5	-0.19050655238643088
2213	6	-0.4865368604983331
2213	7	-0.5799124101667559
2213	8	-0.4849866131580711
2213	9	-0.18172627648752382
2213	10	-3.7292772861032795
2213	11	-1.472184749430248
2213	12	-1.034267243507351
2213	13	-0.7884007358774952
2213	14	-0.09392624383921344
2213	15	-0.49289913732842905
2213	16	-0.3121322049270816
2213	17	-0.011579888121168835
2213	18	0.11767088689519056
2213	19	-0.5115793509303543
2213	20	-0.8327887541181198
2213	21	-0.8537143922140302
2213	22	-1.0702118549072708
2213	23	-1.3787380985136508
2213	24	-0.8804989216262484
2213	25	-0.5865081041636131
2214	1	-0.9979212073676145
2214	2	-0.7831428804646521
2214	3	-0.7335646232864228
2214	4	-0.3812140505452746
2214	5	-3.4747868822350476
2214	6	-1.4540621825589142
2214	7	-1.1503123397876465
2214	8	-0.9079712253422798
2214	9	-0.8809011040959689
2214	10	-4.416530760747843
2214	11	-2.0074602535141466
2214	12	-1.4856740642541553
2214	13	-1.1099626336897472
2214	14	-0.35282942816994733
2214	15	-0.6418039353238569
2214	16	-0.6044370333164295
2214	17	-0.24584082483084302
2214	18	-0.015068020537157167
2214	19	-0.7279193747076036
2214	20	-0.9360998288155642
2214	21	-0.9745241699851874
2214	22	-0.7023041623602992
2214	23	-0.5117479752432743
2214	24	-0.8008251184356681
2214	25	-0.5184276555259402
2215	1	-2.440917022614869
2215	2	-5.0878959306189415
2215	3	-3.1438068103853674
2215	4	-2.1235489752634535
2215	5	-2.201507412620611
2215	6	-2.2733170519367043
2215	7	-2.4637636612201312
2215	8	-2.6689916129405056
2215	9	-8.471116020270994
2215	10	-4.147567391752531
2215	11	-3.4692808015817675
2215	12	-3.7484978312160955
2215	13	-3.1171416109318635
2215	14	-7.8414151553935305
2215	15	-4.7346302817108565
2215	16	-9.63853265963823
2215	17	-4.794200766803286
2215	18	-3.269808585513637
2215	19	-3.7727422721434536
2215	20	-3.500790003107383
2215	21	-3.5746199636210427
2215	22	-9.397682143196501
2215	23	-10.56876784568896
2215	24	-6.85286248397442
2215	25	-5.142537928165467
2216	1	-1.7301561124984062
2216	2	-1.6708838727186084
2216	3	-1.63810068485461
2216	4	-6.833369425181392
2216	5	-3.4624448757970363
2216	6	-2.5722516268850093
2216	7	-2.0542520883719675
2216	8	-7.796284123631292
2216	9	-3.7927279883199496
2216	10	-2.123776490812414
2216	11	-2.0425270878756407
2216	12	-2.1594776039849037
2216	13	-1.9700990655203867
2216	14	-1.3979568972266065
2216	15	-1.8724798842645214
2216	16	-1.622240094550456
2216	17	-1.3528945987628376
2216	18	-7.082485662904247
2216	19	-4.43355733468443
2216	20	-3.8104899940807435
2216	21	-2.9722081464014423
2216	22	-2.630354812504166
2216	23	-2.030676317345538
2216	24	-2.34950409986439
2216	25	-1.691723922437923
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	99416.0
3	5.0
4	880.0
5	4926.0
6	5897.0
7	5298.0
8	4869.0
9	4660.0
10	4188.0
11	4094.0
12	4011.0
13	3978.0
14	3983.0
15	4085.0
16	4159.0
17	4482.0
18	5240.0
19	6022.0
20	7884.0
21	10013.0
22	12539.0
23	14832.0
24	17324.0
25	19784.0
26	22410.0
27	25214.0
28	28347.0
29	33646.0
30	40342.0
31	48267.0
32	61854.0
33	80147.0
34	105795.0
35	141961.0
36	202221.0
37	294736.0
38	801826.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.59525949040786	18.543364218414826	12.118230845341554	25.743145445835758
2	14.539661205043778	20.473540606936552	41.01016122425834	23.97663696376133
3	18.300591108118	25.117103893590226	29.91546961766135	26.666835380630417
4	11.355118528435696	16.86982182110356	39.33075024102026	32.44430940944048
5	12.602946179779387	37.99080736423101	36.44676697211936	12.959479483870243
6	31.532780374367025	37.29560333570681	18.426007180269266	12.745609109656897
7	27.573374201061927	31.65054725884354	22.92867518524423	17.847403354850293
8	24.99038088875445	36.183333013865074	21.272611924144837	17.55367417323564
9	26.574402811114673	14.758071528955025	20.200819285084467	38.466706374845835
10	13.706159985225947	28.728332752812797	35.96443941586387	21.601067846097383
11	34.26715059346176	22.386648033957783	24.72435481751992	18.621846555060543
12	22.808297190692873	24.781601198458166	32.06310420863047	20.346997402218495
13	28.736317403052336	21.76306144176267	25.929546928426184	23.571074226758814
14	20.499450857471214	21.47986547527596	28.928145787172088	29.092537880080737
15	23.303070192871992	29.98548023933055	23.6015553212234	23.10989424657406
16	22.261931478156793	27.83788711849192	27.645823982645123	22.254357420706164
17	21.592652605047125	28.03901900680167	28.244027786545832	22.124300601605373
18	21.47870925750324	27.400550621038438	29.603689385395448	21.517050736062874
19	23.26982330965929	27.01571463972283	27.67081079311941	22.04365125749847
20	23.575123719322658	27.194124314073143	27.65856272373279	21.572189242871413
21	23.37751484380918	26.735082076983	26.93558689274582	22.951816186461997
22	23.144416625593074	27.43843871087214	27.23226018243222	22.184884481102564
23	22.430623190797856	27.305503902102146	27.663334921511428	22.60053798558857
24	22.978247388835523	27.01048207048718	27.676098690653124	22.335171850024178
25	22.852918373817076	26.999505273189268	27.563297443038614	22.584278909955042
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	206.0
1	206.0
2	331.5
3	457.0
4	457.0
5	457.0
6	894.5
7	1332.0
8	1332.0
9	1332.0
10	2261.0
11	3190.0
12	3190.0
13	3190.0
14	5865.5
15	8541.0
16	8541.0
17	8541.0
18	16381.0
19	24221.0
20	24221.0
21	24221.0
22	38853.5
23	53486.0
24	53486.0
25	53486.0
26	76273.5
27	99061.0
28	99061.0
29	99061.0
30	127890.5
31	156720.0
32	156720.0
33	156720.0
34	180534.5
35	204349.0
36	204349.0
37	204349.0
38	224581.5
39	244814.0
40	244814.0
41	244814.0
42	256928.0
43	269042.0
44	269042.0
45	269042.0
46	275872.5
47	282703.0
48	282703.0
49	282703.0
50	271626.0
51	260549.0
52	260549.0
53	260549.0
54	233175.0
55	205801.0
56	205801.0
57	205801.0
58	175583.0
59	145365.0
60	145365.0
61	145365.0
62	118291.5
63	91218.0
64	91218.0
65	91218.0
66	70521.0
67	49824.0
68	49824.0
69	49824.0
70	36443.0
71	23062.0
72	23062.0
73	23062.0
74	16374.0
75	9686.0
76	9686.0
77	9686.0
78	6745.0
79	3804.0
80	3804.0
81	3804.0
82	2561.0
83	1318.0
84	1318.0
85	1318.0
86	870.5
87	423.0
88	423.0
89	423.0
90	278.0
91	133.0
92	133.0
93	133.0
94	77.0
95	21.0
96	21.0
97	21.0
98	15.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17687739414350723
2	0.25881874507732544
3	0.25923943655388243
4	0.4602364753533224
5	0.25489229129612706
6	0.019538781911201376
7	0.01879088595287788
8	0.017014633051859573
9	0.018557168465901785
10	0.021034573827848374
11	0.020754112843477064
12	0.02743843297099332
13	0.027017741494436358
14	0.02552194957778936
15	0.040666842733840185
16	0.021268291314824467
17	0.04160171268174456
18	0.030803964783449062
19	0.026410076028298514
20	0.029121198877221192
21	0.03426298359069524
22	0.04192891716351109
23	0.028793994395454662
24	0.04753813685093732
25	0.03650667146566573
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2139335.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.52109089114357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.63969760082753	63.92082295511003
2	10.693439653289627	16.151604559900896
3	2.543436580111452	5.762493154273692
4	0.8798793520432936	2.6579779407560835
5	0.3961930661504247	1.4960466279593543
6	0.22768822766887653	1.0317158001974769
7	0.1433014108362535	0.7575595210815667
8	0.09267232169660716	0.5598971863954213
9	0.06040464292705336	0.41056420758669626
>10	0.29806222082120015	3.818826429243626
>50	0.014333146008038753	0.7463130951642932
>100	0.009140829172253296	1.371966071182841
>500	0.0010630758430590704	0.4987093389437442
>1k	6.878726043320159E-4	0.8155031122042723
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2168	0.10133990235283394	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.674349739521861E-5	0.0
10	0.0	0.0	0.0	4.674349739521861E-5	0.0
11	0.0	0.0	0.0	4.674349739521861E-5	0.0
12	0.0	0.0	0.0	4.674349739521861E-5	0.0
13	0.0	0.0	0.0	4.674349739521861E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGGG	55	1.8982122E-4	13.871032	2
TAGGACC	550	0.0	13.515044	4
GAGCCGT	55	0.0030957025	12.07698	11
TGCGTGA	55	0.0030957025	12.07698	10
GGCGAGG	600	0.0	11.861597	19
TGGCGAG	1275	0.0	11.387134	18
GGACCGT	75	2.1009389E-4	11.384471	6
AGGCGTG	170	1.2732926E-11	11.161768	7
GTCCTAC	1085	0.0	11.093486	1
TTAGGAC	935	0.0	10.905806	3
CGCAGAT	115	8.971292E-7	10.725003	8
GTATTAG	205	0.0	10.71769	1
GTATTAC	155	3.3451215E-9	10.4771805	1
AGGACGT	1050	0.0	10.416161	5
TAGGACT	400	0.0	10.244576	4
GGACGTG	1050	0.0	10.209883	6
ATTTAGA	590	0.0	10.200366	1
TTTAGAA	715	0.0	10.136523	2
ATGGCGA	1505	0.0	10.087796	17
TGGACCG	95	1.6264086E-4	10.010954	5
>>END_MODULE