FastQCFastQC Report
Thu 26 May 2016
SRR1513133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139335
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31910.14915850018814258No Hit
TATCAACGCAGAGTACTTTTTTTTT22410.10475217766268491No Hit
GTACATGGGAAGCAGTGGTATCAAC21940.10255523328510961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA500.001510270613.2878077
TAGGACC4950.012.4998434
GGACCGT802.8098379E-511.8986096
ACCGTAT658.1018556E-411.6791318
GTCCTAA8100.011.2877321
GTACCGT855.218139E-511.1986916
ACCGTCC855.386302E-511.1638768
TGTAGGA12750.011.0514492
TGGCGAG12750.010.86871218
TGTACCG1158.595125E-710.7607345
GGCGAGG6450.010.73856519
GTAGGAC12700.010.5694533
GCACCGC1105.905824E-610.3842416
TATGGCG13100.010.36144916
TTAGGAC8950.010.2114013
TATGCCG750.00260447710.1537185
ATGGCGA13800.010.11099717
TTAAGAC2550.010.0800053
AGGACCT8250.010.0383355
TAGACTG2300.09.9329855
Produced by FastQC (version 0.11.5)