##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513133_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2139335 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.766600368806195 31.0 31.0 34.0 27.0 34.0 2 30.87273194707701 33.0 31.0 34.0 27.0 34.0 3 30.985655355519356 33.0 31.0 34.0 27.0 34.0 4 34.37633984392346 37.0 35.0 37.0 31.0 37.0 5 34.12427974113451 37.0 35.0 37.0 30.0 37.0 6 34.0353198540668 37.0 35.0 37.0 30.0 37.0 7 33.90974765522931 37.0 35.0 37.0 29.0 37.0 8 33.870976728749824 37.0 35.0 37.0 29.0 37.0 9 35.42888748139025 39.0 35.0 39.0 30.0 39.0 10 35.27953265851304 39.0 35.0 39.0 29.0 39.0 11 35.29711288788339 39.0 35.0 39.0 29.0 39.0 12 35.23275503836472 39.0 35.0 39.0 29.0 39.0 13 35.200034590188075 39.0 35.0 39.0 29.0 39.0 14 36.36783159252759 40.0 37.0 41.0 28.0 41.0 15 36.350418237442945 40.0 37.0 41.0 28.0 41.0 16 36.310810134925106 40.0 37.0 41.0 28.0 41.0 17 36.24928774595844 40.0 36.0 41.0 27.0 41.0 18 36.2028980968385 40.0 36.0 41.0 27.0 41.0 19 36.2835764384727 40.0 37.0 41.0 27.0 41.0 20 36.24951258218091 40.0 37.0 41.0 27.0 41.0 21 36.19937784404967 40.0 37.0 41.0 27.0 41.0 22 36.13997153321009 40.0 37.0 41.0 27.0 41.0 23 36.05746178134794 40.0 37.0 41.0 27.0 41.0 24 35.99792832819544 40.0 37.0 41.0 27.0 41.0 25 35.94308184552676 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5594097316978299 1101 2 0.5397914617008475 1101 3 0.4952834684877452 1101 4 0.7079178784701838 1101 5 0.6860250065587508 1101 6 0.6046181242065671 1101 7 0.7877527588894111 1101 8 0.7596294478739196 1101 9 0.8122574604562729 1101 10 0.9022302685532253 1101 11 0.9391461315632341 1101 12 0.836207069228422 1101 13 0.9045810466315274 1101 14 1.0146906637100699 1101 15 1.0576020122349021 1101 16 0.8927106129416416 1101 17 0.9574155877830393 1101 18 0.995778118781864 1101 19 0.9831951910165486 1101 20 0.9161177932320257 1101 21 0.9679349130297865 1101 22 0.9172038252076078 1101 23 0.9497308639962583 1101 24 0.9658492210114886 1101 25 0.879629475353596 1102 1 0.2597145922657269 1102 2 0.33853298038587454 1102 3 0.30091467455200416 1102 4 0.38326362550173343 1102 5 0.406726111347119 1102 6 0.39595195335920863 1102 7 0.3560507994233646 1102 8 0.4312603794643124 1102 9 0.4078836593388786 1102 10 0.2859214494346034 1102 11 0.48804160193713386 1102 12 0.5624268375153463 1102 13 0.601376222680841 1102 14 0.5904238204638332 1102 15 0.42431835052994415 1102 16 0.5227452989039989 1102 17 0.5561275477437988 1102 18 0.5610580852322968 1102 19 0.5956189422380689 1102 20 0.4669786703768182 1102 21 0.5654977465357192 1102 22 0.5542656424771479 1102 23 0.5399093982768264 1102 24 0.6169334238398108 1102 25 0.5550580199321047 1103 1 0.45246940519635004 1103 2 0.46919502112638156 1103 3 0.47805678695841536 1103 4 0.4500748024264354 1103 5 0.40284880225395625 1103 6 0.2774862987636908 1103 7 0.4069197085456864 1103 8 0.34475835662030363 1103 9 0.4432345436278098 1103 10 0.5839552310793081 1103 11 0.43497417946284145 1103 12 0.45572254025764636 1103 13 0.49252594309793807 1103 14 0.5079119585385428 1103 15 0.484680894509701 1103 16 0.4670468309923663 1103 17 0.5463485875185725 1103 18 0.5592614442219457 1103 19 0.4670740217187088 1103 20 0.46356984616301133 1103 21 0.39106747348423454 1103 22 0.5336743788063458 1103 23 0.5022080002124838 1103 24 0.5894753762562885 1103 25 0.5354999996127887 1104 1 0.40338098334120787 1104 2 0.4702395265223558 1104 3 0.44970874000417993 1104 4 0.5486439113679822 1104 5 0.4490376167663044 1104 6 0.48233900086652426 1104 7 0.46838011503947286 1104 8 0.5580378527020642 1104 9 0.558097505578182 1104 10 0.6903635690918009 1104 11 0.5970676195299447 1104 12 0.6907503653169584 1104 13 0.658052169404499 1104 14 0.6822634176025346 1104 15 0.7344721208211595 1104 16 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-1.2311243252587403 2215 20 -1.2145814738171694 2215 21 -1.0858350359329592 2215 22 -1.4038331903382826 2215 23 -1.287210138996869 2215 24 -1.3517275082759141 2215 25 -1.3744692519659196 2216 1 -0.45781041963946123 2216 2 -0.3514256144648833 2216 3 -0.49646547269453833 2216 4 -0.20312610671243903 2216 5 -0.5420638794523001 2216 6 -0.5781896915201017 2216 7 -0.7741782291082586 2216 8 -0.5936049026035022 2216 9 -0.8458432022029854 2216 10 -0.8664372515336822 2216 11 -0.8741651920581432 2216 12 -0.8345125255660193 2216 13 -0.8077444638169027 2216 14 -0.9822755814730826 2216 15 -0.8977393740859156 2216 16 -0.9152104207444651 2216 17 -1.057890266245245 2216 18 -0.9878267066043804 2216 19 -0.9356415134472726 2216 20 -0.9318695037968752 2216 21 -1.194126989758665 2216 22 -1.0570025856735086 2216 23 -1.1400230043145214 2216 24 -1.2609888105101774 2216 25 -1.369815122693474 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51049.0 3 0.0 4 870.0 5 6998.0 6 7781.0 7 6194.0 8 4838.0 9 4220.0 10 3865.0 11 3476.0 12 3212.0 13 2997.0 14 3034.0 15 3006.0 16 3027.0 17 3345.0 18 3673.0 19 4289.0 20 5682.0 21 7285.0 22 9036.0 23 10987.0 24 12828.0 25 14386.0 26 15775.0 27 17674.0 28 19815.0 29 23791.0 30 30447.0 31 38722.0 32 49202.0 33 64711.0 34 85697.0 35 115896.0 36 167144.0 37 272998.0 38 1061385.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.90553998393664 19.413476826386987 13.081658506201718 27.599324683474645 2 13.919877632381045 20.799690341110956 39.42728064893733 25.853151377570672 3 16.702482186122587 25.10776209644433 30.2411225115148 27.948633205918277 4 11.224902864878409 17.37096641499415 37.88568907038229 33.51844164974515 5 12.584070018114584 38.27146054463524 36.14659902390826 12.997870413341916 6 31.537977732137474 37.8381065703749 18.023845626746198 12.600070070741431 7 27.373515675995147 31.73872343927387 22.88033201725921 18.007428867471774 8 25.043559810061932 36.55321402908725 20.803707130344694 17.599519030506126 9 26.345045067786327 14.653598364265138 19.900505884849725 39.10085068309881 10 14.171188113211691 29.026120848952132 35.282709025457066 21.51998201237911 11 34.24723103207305 22.273276508821667 24.642564161293112 18.836928297812168 12 22.544949715682677 24.98309053981728 32.000878777751026 20.471080966749014 13 28.656942461091884 21.71034456969105 25.885894448508534 23.746818520708537 14 21.022075507153296 21.545192011944128 27.840025728114043 29.59270675278854 15 23.147847345086205 30.21691319966251 23.47257442149079 23.16266503376049 16 22.47572259604036 27.993558746058937 27.106928087466432 22.42379057043427 17 21.86053144015279 28.25539236197961 27.605059598252378 22.279016599615222 18 21.83739559136527 27.589595981132568 28.823703238210662 21.749305189291498 19 23.22427296332739 27.110620823760655 27.53654757202589 22.12855864088607 20 23.40325381485368 27.191487074254383 27.766385348718174 21.63887376217376 21 23.227409920848423 26.730777590749927 27.064903546138407 22.97690894226324 22 23.077099264116434 27.6031828612002 27.015990497972965 22.303727376710405 23 22.478788700395942 27.291170957231476 27.284018717364983 22.946021625007596 24 22.824195369121714 27.045787592873488 27.82275800657681 22.30725903142799 25 22.826736794574373 26.929097861993178 27.487029811683943 22.757135531748506 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 200.0 1 200.0 2 304.0 3 408.0 4 408.0 5 408.0 6 992.0 7 1576.0 8 1576.0 9 1576.0 10 2263.5 11 2951.0 12 2951.0 13 2951.0 14 5552.0 15 8153.0 16 8153.0 17 8153.0 18 15936.0 19 23719.0 20 23719.0 21 23719.0 22 38284.0 23 52849.0 24 52849.0 25 52849.0 26 75162.0 27 97475.0 28 97475.0 29 97475.0 30 125794.5 31 154114.0 32 154114.0 33 154114.0 34 178097.5 35 202081.0 36 202081.0 37 202081.0 38 221206.0 39 240331.0 40 240331.0 41 240331.0 42 253707.5 43 267084.0 44 267084.0 45 267084.0 46 274800.0 47 282516.0 48 282516.0 49 282516.0 50 274048.0 51 265580.0 52 265580.0 53 265580.0 54 236805.0 55 208030.0 56 208030.0 57 208030.0 58 178283.0 59 148536.0 60 148536.0 61 148536.0 62 121003.5 63 93471.0 64 93471.0 65 93471.0 66 72337.5 67 51204.0 68 51204.0 69 51204.0 70 37422.5 71 23641.0 72 23641.0 73 23641.0 74 16715.5 75 9790.0 76 9790.0 77 9790.0 78 6781.5 79 3773.0 80 3773.0 81 3773.0 82 2544.0 83 1315.0 84 1315.0 85 1315.0 86 870.5 87 426.0 88 426.0 89 426.0 90 261.5 91 97.0 92 97.0 93 97.0 94 55.0 95 13.0 96 13.0 97 13.0 98 7.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18884372947668318 2 0.009675903960810252 3 0.006170141656168856 4 0.0021969443775752746 5 0.08558734373064528 6 0.3367401552351548 7 0.030430016804287315 8 0.01505140616126039 9 0.005282015205659703 10 0.0040199407759888 11 0.0 12 0.0 13 0.0 14 0.001916483393203963 15 0.0 16 0.0 17 0.020099703879944003 18 0.029121198877221192 19 0.0 20 0.0 21 0.0010283569426948094 22 9.348699479043722E-5 23 0.006777807122306698 24 0.0 25 2.804609843713117E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2139335.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.79163404912518 #Duplication Level Percentage of deduplicated Percentage of total 1 82.99453842842645 60.413080693580746 2 11.628366503251211 16.92895598187535 3 2.900715104518391 6.334433771066178 4 1.0320242150148036 3.00490915956773 5 0.4701300658097458 1.7110767852957072 6 0.2534276443228354 1.1068447406087811 7 0.16253143615665838 0.8281650175535961 8 0.10555792758505088 0.6146987228604063 9 0.07861165316568612 0.515004362031004 >10 0.34379514304878533 4.34298129300867 >50 0.01678229149622733 0.8557953920870474 >100 0.011461635134015552 1.6447411143666075 >500 0.0012219090416524842 0.5936828384588841 >1k 8.360430284990037E-4 1.1056301276392546 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3191 0.14915850018814258 No Hit TATCAACGCAGAGTACTTTTTTTTT 2241 0.10475217766268491 No Hit GTACATGGGAAGCAGTGGTATCAAC 2194 0.10255523328510961 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.674349739521861E-5 0.0 10 0.0 0.0 0.0 4.674349739521861E-5 0.0 11 0.0 0.0 0.0 4.674349739521861E-5 0.0 12 0.0 4.674349739521861E-5 0.0 4.674349739521861E-5 4.674349739521861E-5 13 0.0 4.674349739521861E-5 0.0 4.674349739521861E-5 4.674349739521861E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCGTA 50 0.0015102706 13.287807 7 TAGGACC 495 0.0 12.499843 4 GGACCGT 80 2.8098379E-5 11.898609 6 ACCGTAT 65 8.1018556E-4 11.679131 8 GTCCTAA 810 0.0 11.287732 1 GTACCGT 85 5.218139E-5 11.198691 6 ACCGTCC 85 5.386302E-5 11.163876 8 TGTAGGA 1275 0.0 11.051449 2 TGGCGAG 1275 0.0 10.868712 18 TGTACCG 115 8.595125E-7 10.760734 5 GGCGAGG 645 0.0 10.738565 19 GTAGGAC 1270 0.0 10.569453 3 GCACCGC 110 5.905824E-6 10.384241 6 TATGGCG 1310 0.0 10.361449 16 TTAGGAC 895 0.0 10.211401 3 TATGCCG 75 0.002604477 10.153718 5 ATGGCGA 1380 0.0 10.110997 17 TTAAGAC 255 0.0 10.080005 3 AGGACCT 825 0.0 10.038335 5 TAGACTG 230 0.0 9.932985 5 >>END_MODULE