FastQCFastQC Report
Thu 26 May 2016
SRR1513132_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513132_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72553
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC28683.952972309897592No Hit
CCCATGTACTCTGCGTTGATACCAC24243.341005885352777No Hit
GAGTACATGGGAAGCAGTGGTATCA11721.6153708323570353No Hit
CATGTACTCTGCGTTGATACCACTG8701.1991233994459223No Hit
TATCAACGCAGAGTACATGGGAAGC8181.1274516560307637No Hit
GCGTTGATACCACTGCTTCCCATGT8041.1081554174189903No Hit
GTATCAACGCAGAGTACATGGGAAG7240.9978911967802848No Hit
GGTATCAACGCAGAGTACATGGGAA6680.920706242333191No Hit
ACGCAGAGTACATGGGAAGCAGTGG6650.9165713340592395No Hit
GTATCAACGCAGAGTACTTTTTTTT6550.9027883064794012No Hit
ACTCTGCGTTGATACCACTGCTTCC5390.7429051865532783No Hit
GGTATCAACGCAGAGTACTTTTTTT4470.616101332818767No Hit
TATCAACGCAGAGTACTTTTTTTTT4300.5926701859330421No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4130.5692390390473171No Hit
GGGAAGCAGTGGTATCAACGCAGAG3580.4934323873582071No Hit
GTACTCTGCGTTGATACCACTGCTT3320.4575965156506278No Hit
ATACCACTGCTTCCCATGTACTCTG3250.44794839634474104No Hit
GCTTCCCATGTACTCTGCGTTGATA3240.4465700935867573No Hit
GTGGTATCAACGCAGAGTACATGGG3070.4231389467010323No Hit
CTGCTTCCCATGTACTCTGCGTTGA2850.3928162860253884No Hit
GTACTTTTTTTTTTTTTTTTTTTTT2790.38454646947748544No Hit
GCAGAGTACATGGGAAGCAGTGGTA2580.35560211155982524No Hit
TACCACTGCTTCCCATGTACTCTGC2550.3514672032858738No Hit
GCAGTGGTATCAACGCAGAGTACAT2540.35008890052788993No Hit
CAGTGGTATCAACGCAGAGTACATG2510.34595399225393847No Hit
GATACCACTGCTTCCCATGTACTCT2430.33492757019006797No Hit
CCACTGCTTCCCATGTACTCTGCGT2380.3280360564001489No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2380.3280360564001489No Hit
CATGGGAAGCAGTGGTATCAACGCA2220.30598321227240777No Hit
GTTGATACCACTGCTTCCCATGTAC2190.3018483039984563No Hit
ACATGGGAAGCAGTGGTATCAACGC2180.30047000124047246No Hit
AAGCAGTGGTATCAACGCAGAGTAC2150.29633509296652105No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1980.2729039460807961No Hit
ATGGGAAGCAGTGGTATCAACGCAG1870.2577426157429741No Hit
CCATGTACTCTGCGTTGATACCACT1660.2287982578253139No Hit
GAACAAAAAAAAAAAAAAAAAAAAA1500.2067454136975728No Hit
GTACATGGGAAGCAGTGTTATCAAC1360.18744917508579934No Hit
CTTCCCATGTACTCTGCGTTGATAC1340.1846925695698317No Hit
CCCATGTACTCTGCGTTTATACCAC1310.18055766129588025No Hit
GTACATGGGAAGCAGTTGTATCAAC1310.18055766129588025No Hit
ATCAACGCAGAGTACATGGGAAGCA1240.17090954198999353No Hit
TGATACCACTGCTTCCCATGTACTC1180.1626397254420906No Hit
TTCCCATGTACTCTGCGTTGATACC1140.15712651441015532No Hit
GGAAGCAGTGGTATCAACGCAGAGT950.13093876200846277No Hit
AAAAAGTACTCTGCGTTGATACCAC900.12404724821854368No Hit
GTACATGGTAAGCAGTGGTATCAAC870.11991233994459224No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA830.11439912891265695No Hit
GCAGAGTACTTTTTTTTTTTTTTTT770.10612931236475404No Hit
GTGGTATCAACGCAGAGTACTTTTT740.1019944040908026No Hit
GTACTGGTTCACTATCGGTCAGTCA730.10061610133281877No Hit
GAGTACATGTGAAGCAGTGGTATCA730.10061610133281877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAT307.7048E-418.97105415
TTATCAA402.747659E-416.59967218
TGTTATC350.002165446816.26090216
AGTGTTA350.002165446816.26090214
CAGTGTT350.002165446816.26090213
GTTATCA350.002165446816.26090217
CCATGTA6950.08.2400542
TGCGTTG6700.08.21135212
TCTGCGT7000.08.13045110
CTCTGCG7150.08.0925479
TACTCTG7150.08.0925477
CTGCGTT7050.08.07278911
TGATACC6950.08.05246217
GTACTCT7250.07.98092656
TGTACTC7200.07.9319385
ACTCTGC7450.07.8939968
GATACCA7200.07.772862418
TTGATAC7100.07.748740716
ATGTACT7400.07.73360734
CGTTGAT7000.07.72392914
Produced by FastQC (version 0.11.5)