##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513132_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72553 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.541328408198144 31.0 28.0 34.0 7.0 34.0 2 27.28044326216697 31.0 27.0 34.0 7.0 34.0 3 27.51866911085689 31.0 27.0 34.0 7.0 34.0 4 30.220156299532757 35.0 30.0 37.0 7.0 37.0 5 30.59727371714471 35.0 31.0 37.0 7.0 37.0 6 30.64647912560473 35.0 31.0 37.0 2.0 37.0 7 30.552354830262015 35.0 31.0 37.0 2.0 37.0 8 30.226799718826236 35.0 31.0 37.0 2.0 37.0 9 31.05114881534878 37.0 30.0 39.0 2.0 39.0 10 30.718536793792126 37.0 30.0 39.0 2.0 39.0 11 31.140834975810787 37.0 31.0 39.0 2.0 39.0 12 31.14563146940857 37.0 31.0 39.0 2.0 39.0 13 31.078067068212203 37.0 31.0 39.0 2.0 39.0 14 31.1923008007939 38.0 28.0 41.0 2.0 41.0 15 31.550494121538737 38.0 30.0 41.0 2.0 41.0 16 31.334155720645597 38.0 30.0 41.0 2.0 41.0 17 31.107369784846938 38.0 29.0 41.0 2.0 41.0 18 30.86804129395063 38.0 27.0 41.0 2.0 41.0 19 31.397888440174768 38.0 30.0 41.0 2.0 41.0 20 31.693961655617272 39.0 31.0 41.0 2.0 41.0 21 31.766956569680097 39.0 31.0 41.0 2.0 41.0 22 31.523231292985816 39.0 30.0 41.0 2.0 41.0 23 31.088004631097267 38.0 30.0 41.0 2.0 41.0 24 31.288368503025374 38.0 30.0 41.0 2.0 41.0 25 30.963089052141193 38.0 30.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.146387158991004 1101 2 2.480849218005602 1101 3 2.3356030659430473 1101 4 3.0294788535301684 1101 5 2.8261836091224133 1101 6 2.0490674878562665 1101 7 2.626484938726172 1101 8 2.7267784923218343 1101 9 2.493047876586605 1101 10 3.4927757639184165 1101 11 2.9418028125008746 1101 12 3.449571951674976 1101 13 2.797758287531366 1101 14 3.0627327970093816 1101 15 2.7550997304909544 1101 16 2.483834954011723 1101 17 3.2547955262511152 1101 18 3.585984399439493 1101 19 2.686651345995241 1101 20 2.5732287112706302 1101 21 2.6690222794159766 1101 22 3.014594691724721 1101 23 3.0644842729034387 1101 24 2.8911919665484653 1101 25 2.726286895076637 1102 1 2.905356009689175 1102 2 3.4712896046865893 1102 3 3.3257212184672156 1102 4 4.106170582853476 1102 5 3.7462695382309015 1102 6 3.5621716769003022 1102 7 3.788998365149375 1102 8 3.4872511024185044 1102 9 3.9385903040839203 1102 10 4.457867063596183 1102 11 4.055873704015369 1102 12 4.374061747593345 1102 13 3.9469527021393134 1102 14 5.043720981756962 1102 15 4.952307034465171 1102 16 4.45354494327059 1102 17 4.9924969225991305 1102 18 5.646994066464199 1102 19 4.6884773395505555 1102 20 3.9047002472534444 1102 21 3.585563632799442 1102 22 4.12243572287402 1102 23 4.623345712216004 1102 24 4.734800989104858 1102 25 4.684503866075563 1103 1 1.4905247306429033 1103 2 1.8484365030498964 1103 3 1.8015749262869747 1103 4 1.6238509223159987 1103 5 1.2948652141250214 1103 6 0.9853884883773709 1103 7 0.862649607824661 1103 8 0.2619530624104236 1103 9 0.9220213002447615 1103 10 1.4646361078475465 1103 11 0.5097027603903967 1103 12 1.2139283873185462 1103 13 1.2060959634042163 1103 14 2.1644524426581597 1103 15 2.0585911322627055 1103 16 2.1775295866328825 1103 17 2.1715229884710254 1103 18 2.377646723566638 1103 19 1.8783136701223846 1103 20 1.5135101078313369 1103 21 1.2001322325165518 1103 22 1.5506030294005946 1103 23 2.1886114224605002 1103 24 1.8748292776480007 1103 25 1.6947079477082134 1104 1 -0.4375774253670883 1104 2 -0.02146263639675894 1104 3 -0.16788930985626394 1104 4 0.0020317310510762354 1104 5 0.11285355501518524 1104 6 -0.25806482891697513 1104 7 -0.6753350317607918 1104 8 -0.7582190482585851 1104 9 -0.014871454451267141 1104 10 -0.11174956810322456 1104 11 -0.49075990269834335 1104 12 -0.9392130950539936 1104 13 0.06396586254366099 1104 14 0.9837362401967908 1104 15 0.8794479843030487 1104 16 0.9055762230722273 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2216 25 -2.1347494166328715 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6693.0 3 0.0 4 41.0 5 292.0 6 609.0 7 524.0 8 443.0 9 318.0 10 309.0 11 297.0 12 301.0 13 298.0 14 232.0 15 191.0 16 165.0 17 173.0 18 213.0 19 304.0 20 386.0 21 545.0 22 716.0 23 777.0 24 809.0 25 910.0 26 970.0 27 995.0 28 1058.0 29 1186.0 30 1411.0 31 1599.0 32 1935.0 33 2474.0 34 3208.0 35 4412.0 36 6313.0 37 9309.0 38 22137.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.25428784489187 18.093186400419807 10.840445217775569 21.812080536912752 2 13.818699546610638 24.357237642375317 35.97948689594161 25.844575915072433 3 21.876296071003953 31.823209002682002 26.001603671855563 20.298891254458486 4 12.122764982064457 20.091962937828068 31.53193080619919 36.25334127390828 5 11.2358307990047 42.00165883328725 32.36521979541056 14.397290572297482 6 30.85778096393515 32.036506010808424 19.182199183853534 17.923513841402887 7 24.502005762258587 34.620421554706994 21.579520547001007 19.298052136033416 8 29.531700188863923 31.58990336232923 21.34438026440949 17.53401618439736 9 29.845046734125564 14.894262317681767 18.413796906449033 36.84689404174364 10 17.54204576785222 31.874827681279292 29.033636614281775 21.54948993658671 11 29.695599426491675 28.154295797948603 17.27555972206904 24.874545053490678 12 25.781411573301718 32.06165809538254 25.766245226047513 16.390685105268236 13 30.794578720236043 23.10661942119704 21.301824097947026 24.79697776061989 14 22.065048048420675 28.842839613406678 25.44015662268547 23.651955715487173 15 33.183949255377826 24.43050193050193 21.678157749586322 20.707391064533923 16 18.743796183963827 29.747987206352704 30.44832910554759 21.059887504135876 17 24.25119973523085 30.976612058028575 25.79706547520547 18.97512273153511 18 23.913193343352315 25.551158846806103 30.112093093796965 20.423554716044617 19 22.176418684166986 31.08421110682182 30.168753102079084 16.57061710693211 20 25.100304705703923 26.867873540239078 27.16706420879924 20.86475754525776 21 21.495932717496206 28.836343581966084 29.886943333792914 19.780780366744796 22 22.52833622900637 29.23388764788616 22.52695733708392 25.710818786023555 23 18.951562866242917 30.159802556289385 28.092986060363728 22.795648517103974 24 21.52863958522931 34.69429966079259 24.3526102424092 19.424450511568903 25 21.55789357565395 26.49439472704458 23.480095420636783 28.467616276664693 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 478.0 1 478.0 2 366.0 3 254.0 4 254.0 5 254.0 6 475.5 7 697.0 8 697.0 9 697.0 10 675.5 11 654.0 12 654.0 13 654.0 14 578.5 15 503.0 16 503.0 17 503.0 18 689.5 19 876.0 20 876.0 21 876.0 22 1165.5 23 1455.0 24 1455.0 25 1455.0 26 1997.5 27 2540.0 28 2540.0 29 2540.0 30 2945.5 31 3351.0 32 3351.0 33 3351.0 34 3656.5 35 3962.0 36 3962.0 37 3962.0 38 4448.0 39 4934.0 40 4934.0 41 4934.0 42 5963.0 43 6992.0 44 6992.0 45 6992.0 46 12180.5 47 17369.0 48 17369.0 49 17369.0 50 15043.0 51 12717.0 52 12717.0 53 12717.0 54 9639.0 55 6561.0 56 6561.0 57 6561.0 58 5101.0 59 3641.0 60 3641.0 61 3641.0 62 3155.0 63 2669.0 64 2669.0 65 2669.0 66 2045.5 67 1422.0 68 1422.0 69 1422.0 70 1134.0 71 846.0 72 846.0 73 846.0 74 622.0 75 398.0 76 398.0 77 398.0 78 282.0 79 166.0 80 166.0 81 166.0 82 106.5 83 47.0 84 47.0 85 47.0 86 30.5 87 14.0 88 14.0 89 14.0 90 10.0 91 6.0 92 6.0 93 6.0 94 3.0 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1915840833597508 2 0.2880652764186181 3 0.3018483039984563 4 0.48240596529433655 5 0.29357848745055337 6 0.023431146885724917 7 0.016539633095805823 8 0.019296238611773462 9 0.02067454136975728 10 0.017917935853789643 11 0.023431146885724917 12 0.033079266191611646 13 0.033079266191611646 14 0.03032266067564401 15 0.04548399101346602 16 0.023431146885724917 17 0.0509972020454013 18 0.033079266191611646 19 0.02894435791766019 20 0.033079266191611646 21 0.031700963433627834 22 0.04272738549749838 23 0.035835871707579285 24 0.04272738549749838 25 0.044105688255482195 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 72553.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.16875938968754 #Duplication Level Percentage of deduplicated Percentage of total 1 85.95772787318361 44.84308023100354 2 8.343461030383091 8.705360219425799 3 2.478203434610304 3.8785439609664656 4 1.0620871862615586 2.2163108348379805 5 0.5072655217965654 1.323170647664466 6 0.3408190224570674 1.0668063346794756 7 0.190224570673712 0.6946645900238446 8 0.15059445178335534 0.6285060576406213 9 0.1083223249669749 0.5085937176960291 >10 0.6948480845442536 6.804680716166113 >50 0.05284015852047556 1.892409686711783 >100 0.0845442536327609 10.612931236475404 >500 0.021136063408190225 7.915592739101071 >1k 0.007926023778071334 8.909349027607403 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2868 3.952972309897592 No Hit CCCATGTACTCTGCGTTGATACCAC 2424 3.341005885352777 No Hit GAGTACATGGGAAGCAGTGGTATCA 1172 1.6153708323570353 No Hit CATGTACTCTGCGTTGATACCACTG 870 1.1991233994459223 No Hit TATCAACGCAGAGTACATGGGAAGC 818 1.1274516560307637 No Hit GCGTTGATACCACTGCTTCCCATGT 804 1.1081554174189903 No Hit GTATCAACGCAGAGTACATGGGAAG 724 0.9978911967802848 No Hit GGTATCAACGCAGAGTACATGGGAA 668 0.920706242333191 No Hit ACGCAGAGTACATGGGAAGCAGTGG 665 0.9165713340592395 No Hit GTATCAACGCAGAGTACTTTTTTTT 655 0.9027883064794012 No Hit ACTCTGCGTTGATACCACTGCTTCC 539 0.7429051865532783 No Hit GGTATCAACGCAGAGTACTTTTTTT 447 0.616101332818767 No Hit TATCAACGCAGAGTACTTTTTTTTT 430 0.5926701859330421 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 413 0.5692390390473171 No Hit GGGAAGCAGTGGTATCAACGCAGAG 358 0.4934323873582071 No Hit GTACTCTGCGTTGATACCACTGCTT 332 0.4575965156506278 No Hit ATACCACTGCTTCCCATGTACTCTG 325 0.44794839634474104 No Hit GCTTCCCATGTACTCTGCGTTGATA 324 0.4465700935867573 No Hit GTGGTATCAACGCAGAGTACATGGG 307 0.4231389467010323 No Hit CTGCTTCCCATGTACTCTGCGTTGA 285 0.3928162860253884 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 279 0.38454646947748544 No Hit GCAGAGTACATGGGAAGCAGTGGTA 258 0.35560211155982524 No Hit TACCACTGCTTCCCATGTACTCTGC 255 0.3514672032858738 No Hit GCAGTGGTATCAACGCAGAGTACAT 254 0.35008890052788993 No Hit CAGTGGTATCAACGCAGAGTACATG 251 0.34595399225393847 No Hit GATACCACTGCTTCCCATGTACTCT 243 0.33492757019006797 No Hit CCACTGCTTCCCATGTACTCTGCGT 238 0.3280360564001489 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 238 0.3280360564001489 No Hit CATGGGAAGCAGTGGTATCAACGCA 222 0.30598321227240777 No Hit GTTGATACCACTGCTTCCCATGTAC 219 0.3018483039984563 No Hit ACATGGGAAGCAGTGGTATCAACGC 218 0.30047000124047246 No Hit AAGCAGTGGTATCAACGCAGAGTAC 215 0.29633509296652105 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 198 0.2729039460807961 No Hit ATGGGAAGCAGTGGTATCAACGCAG 187 0.2577426157429741 No Hit CCATGTACTCTGCGTTGATACCACT 166 0.2287982578253139 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 150 0.2067454136975728 No Hit GTACATGGGAAGCAGTGTTATCAAC 136 0.18744917508579934 No Hit CTTCCCATGTACTCTGCGTTGATAC 134 0.1846925695698317 No Hit CCCATGTACTCTGCGTTTATACCAC 131 0.18055766129588025 No Hit GTACATGGGAAGCAGTTGTATCAAC 131 0.18055766129588025 No Hit ATCAACGCAGAGTACATGGGAAGCA 124 0.17090954198999353 No Hit TGATACCACTGCTTCCCATGTACTC 118 0.1626397254420906 No Hit TTCCCATGTACTCTGCGTTGATACC 114 0.15712651441015532 No Hit GGAAGCAGTGGTATCAACGCAGAGT 95 0.13093876200846277 No Hit AAAAAGTACTCTGCGTTGATACCAC 90 0.12404724821854368 No Hit GTACATGGTAAGCAGTGGTATCAAC 87 0.11991233994459224 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 83 0.11439912891265695 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 77 0.10612931236475404 No Hit GTGGTATCAACGCAGAGTACTTTTT 74 0.1019944040908026 No Hit GTACTGGTTCACTATCGGTCAGTCA 73 0.10061610133281877 No Hit GAGTACATGTGAAGCAGTGGTATCA 73 0.10061610133281877 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTTAT 30 7.7048E-4 18.971054 15 TTATCAA 40 2.747659E-4 16.599672 18 TGTTATC 35 0.0021654468 16.260902 16 AGTGTTA 35 0.0021654468 16.260902 14 CAGTGTT 35 0.0021654468 16.260902 13 GTTATCA 35 0.0021654468 16.260902 17 CCATGTA 695 0.0 8.240054 2 TGCGTTG 670 0.0 8.211352 12 TCTGCGT 700 0.0 8.130451 10 CTCTGCG 715 0.0 8.092547 9 TACTCTG 715 0.0 8.092547 7 CTGCGTT 705 0.0 8.072789 11 TGATACC 695 0.0 8.052462 17 GTACTCT 725 0.0 7.9809265 6 TGTACTC 720 0.0 7.931938 5 ACTCTGC 745 0.0 7.893996 8 GATACCA 720 0.0 7.7728624 18 TTGATAC 710 0.0 7.7487407 16 ATGTACT 740 0.0 7.7336073 4 CGTTGAT 700 0.0 7.723929 14 >>END_MODULE