FastQCFastQC Report
Thu 26 May 2016
SRR1513132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72553
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC33814.660041624743291No Hit
GTACATGGGAAGCAGTGGTATCAAC31994.409190522790237No Hit
GAGTACATGGGAAGCAGTGGTATCA13901.915840833597508No Hit
GTATCAACGCAGAGTACTTTTTTTT12951.7849020715890451No Hit
CATGTACTCTGCGTTGATACCACTG11281.5547255110057474No Hit
TATCAACGCAGAGTACATGGGAAGC9751.3438451890342233No Hit
TATCAACGCAGAGTACTTTTTTTTT9581.3204140421484984No Hit
GCGTTGATACCACTGCTTCCCATGT8731.2032583077198737No Hit
GGTATCAACGCAGAGTACTTTTTTT8651.1922318856560032No Hit
GTATCAACGCAGAGTACATGGGAAG8201.1302082615467315No Hit
ACGCAGAGTACATGGGAAGCAGTGG7831.0792110595013302No Hit
ACTCTGCGTTGATACCACTGCTTCC7371.0158091326340744No Hit
GGTATCAACGCAGAGTACATGGGAA6310.8697090402877895No Hit
GTACTTTTTTTTTTTTTTTTTTTTT5370.7401485810373106No Hit
CTTATACACATCTCCGAGCCCACGA5010.6905296817498932No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4860.6698551403801359No Hit
ATACCACTGCTTCCCATGTACTCTG4370.6023183052389288No Hit
GTACTCTGCGTTGATACCACTGCTT4100.5651041307733657No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3900.5375380756136893No Hit
GCTTCCCATGTACTCTGCGTTGATA3580.4934323873582071No Hit
CAGTGGTATCAACGCAGAGTACATG3530.486540873568288No Hit
TACCACTGCTTCCCATGTACTCTGC3450.4755144515044174No Hit
GTGGTATCAACGCAGAGTACATGGG3430.47275784598844983No Hit
GGGAAGCAGTGGTATCAACGCAGAG3360.46310972668256306No Hit
CTGCTTCCCATGTACTCTGCGTTGA3230.44519179082877347No Hit
GCAGAGTACATGGGAAGCAGTGGTA3080.42451724945901614No Hit
CATGGGAAGCAGTGGTATCAACGCA3010.41486913015312943No Hit
ATACACATCTCCGAGCCCACGAGAC2720.37489835017159867No Hit
ACATGGGAAGCAGTGGTATCAACGC2530.34871059776990615No Hit
CCACTGCTTCCCATGTACTCTGCGT2530.34871059776990615No Hit
GCAGTGGTATCAACGCAGAGTACAT2530.34871059776990615No Hit
GATACCACTGCTTCCCATGTACTCT2520.3473322950119223No Hit
ATGGGAAGCAGTGGTATCAACGCAG2390.32941435915813266No Hit
GTTGATACCACTGCTTCCCATGTAC2350.32390114812619736No Hit
AAGCAGTGGTATCAACGCAGAGTAC2330.3211445426102298No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT2280.31425302882031064No Hit
TCTCCGAGCCCACGAGACCAGAGAG2170.2990916984824887No Hit
CCATGTACTCTGCGTTGATACCACT2150.29633509296652105No Hit
CTTCCCATGTACTCTGCGTTGATAC2010.27703885435474757No Hit
ACACATCTCCGAGCCCACGAGACCA1960.2701473405648285No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1930.266012432290877No Hit
ATCAACGCAGAGTACATGGGAAGCA1930.266012432290877No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1580.21777183576144338No Hit
TTCCCATGTACTCTGCGTTGATACC1560.21501523024547572No Hit
ATCAACGCAGAGTACTTTTTTTTTT1460.20123220266563754No Hit
TGATACCACTGCTTCCCATGTACTC1410.19434068887571843No Hit
GTGGTATCAACGCAGAGTACTTTTT1230.1695312392320097No Hit
GGAAGCAGTGGTATCAACGCAGAGT1210.16677463371604206No Hit
TCAACGCAGAGTACATGGGAAGCAG910.1254255509765275No Hit
GTACTGGTTCACTATCGGTCAGTCA890.12266894546055986No Hit
GTACATGGTAAGCAGTGGTATCAAC860.11853403718660842No Hit
AAAGTACTCTGCGTTGATACCACTG840.11577743167064077No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT840.11577743167064077No Hit
AAAAAGTACTCTGCGTTGATACCAC830.11439912891265695No Hit
CATCTCCGAGCCCACGAGACCAGAG820.11302082615467313No Hit
CTCTGCGTTGATACCACTGCTTCCC780.10750761512273786No Hit
CTGCGTTGATACCACTGCTTCCCAT780.10750761512273786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC502.0669904E-719.03111
CCAGAGA250.005993056618.99172618
TATACAC555.6229874E-717.3009993
TTATACA601.3962272E-615.8592492
ATACACA802.7328239E-511.8944374
TACACAT855.0766823E-511.1947635
CCATGTA9250.010.0813392
TGCGTTG9800.010.07724112
CTGCGTT9950.010.01385311
AGTGGTA8100.09.96479314
CGTTGAT10050.09.9210514
GTGGTAT8100.09.84756115
CAGTGGT8200.09.84327213
TGATACC10100.09.77791817
TTGATAC10100.09.77791816
TGGTATC8250.09.66851416
TACTCTG9950.09.6323737
TCTGCGT10350.09.62684310
CCCATGT9400.09.6167781
GTTGATA10400.09.58716915
Produced by FastQC (version 0.11.5)