##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513132_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72553 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.39934944109823 31.0 30.0 34.0 25.0 34.0 2 29.36119802075724 31.0 30.0 34.0 23.0 34.0 3 29.49479690708861 31.0 30.0 34.0 24.0 34.0 4 32.68474081016636 35.0 33.0 37.0 25.0 37.0 5 32.46973936294847 35.0 33.0 37.0 23.0 37.0 6 32.23061761746585 37.0 33.0 37.0 18.0 37.0 7 31.960208399377006 37.0 33.0 37.0 17.0 37.0 8 31.855829531514892 37.0 33.0 37.0 13.0 37.0 9 33.15040039695119 39.0 34.0 39.0 10.0 39.0 10 33.063567323198214 39.0 34.0 39.0 10.0 39.0 11 33.07510371728254 39.0 34.0 39.0 10.0 39.0 12 33.026091271208635 39.0 34.0 39.0 10.0 39.0 13 32.91072733036539 39.0 34.0 39.0 8.0 39.0 14 33.90307775005858 39.0 34.0 41.0 8.0 41.0 15 33.82021418824859 39.0 34.0 41.0 8.0 41.0 16 33.67510647388806 39.0 33.0 41.0 7.0 41.0 17 33.7803536724877 39.0 34.0 41.0 2.0 41.0 18 33.7669565696801 39.0 34.0 41.0 2.0 41.0 19 33.74344272462889 39.0 34.0 41.0 2.0 41.0 20 33.89778506746792 39.0 34.0 41.0 2.0 41.0 21 33.82378399239177 39.0 34.0 41.0 2.0 41.0 22 33.79970504320979 39.0 34.0 41.0 2.0 41.0 23 33.725083731892546 39.0 34.0 41.0 2.0 41.0 24 33.63229639022508 39.0 34.0 41.0 2.0 41.0 25 33.58843879646603 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.0387098922727418 1101 2 0.8818906460589311 1101 3 0.9491746505857712 1101 4 1.1770286005026307 1101 5 1.078518127512396 1101 6 0.9057981762534837 1101 7 1.703447366140253 1101 8 1.4321802328653348 1101 9 1.4656207084372355 1101 10 1.5157909358912534 1101 11 1.7923814542274457 1101 12 1.2869874778671928 1101 13 1.4252551096762005 1101 14 1.3495270477876673 1101 15 1.9565546790533048 1101 16 1.4332449031075427 1101 17 1.50363489895566 1101 18 1.5862645953509613 1101 19 1.598208172728171 1101 20 1.250729304589342 1101 21 1.5668717157500325 1101 22 1.4344751337835646 1101 23 1.4139582791145244 1101 24 1.8783067879239255 1101 25 1.353972064204335 1102 1 2.4505251446894967 1102 2 2.47329773521038 1102 3 2.3514302896835133 1102 4 1.9965774726830858 1102 5 2.180344121067712 1102 6 2.192693987209445 1102 7 2.8310520922412223 1102 8 2.407797848332571 1102 9 3.0256636729914774 1102 10 3.0123537715518367 1102 11 3.3000076626055375 1102 12 2.7017028376953363 1102 13 3.0414742289028425 1102 14 2.5496344591732694 1102 15 2.724116440600028 1102 16 3.770194419756308 1102 17 3.844666048257487 1102 18 3.438788762912722 1102 19 3.520871975091218 1102 20 3.373070872795573 1102 21 3.2525860014643158 1102 22 3.482165788993022 1102 23 3.187427666869631 1102 24 3.448231599953999 1102 25 3.0426938687156095 1103 1 0.8774800850814977 1103 2 0.6434331160954088 1103 3 0.7594925244784214 1103 4 0.5991234415656876 1103 5 0.8008735209464746 1103 6 1.2348237103858395 1103 7 1.495630795009454 1103 8 1.701695605455221 1103 9 0.9682783426217085 1103 10 0.9348633694503974 1103 11 1.2032204328621532 1103 12 1.4185143147614099 1103 13 1.670591222234826 1103 14 1.6586463807735967 1103 15 1.376773543044969 1103 16 1.5128697076932696 1103 17 1.5911804956205913 1103 18 1.4782916927975478 1103 19 1.368922086224785 1103 20 1.6069565062568785 1103 21 1.6104360721856779 1103 22 1.5270233359722098 1103 23 1.4694038236689835 1103 24 1.984299492457538 1103 25 1.9776302194935482 1104 1 -0.21893890261166504 1104 2 -0.16641235443295344 1104 3 -0.6425616996355785 1104 4 0.029955309799241547 1104 5 -0.7260072045092478 1104 6 -0.9029573574405667 1104 7 -0.60476709524686 1104 8 -1.3393888768247812 1104 9 -0.9686144120743236 1104 10 -1.2538106381372742 1104 11 -1.18902533377058 1104 12 -0.8291955029493323 1104 13 -0.7471010142785417 1104 14 -0.6276495385379661 1104 15 -0.6613498954671044 1104 16 -0.9958746246838999 1104 17 -1.2376341615460618 1104 18 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-0.7105300717553789 2216 15 -0.9310014204978998 2216 16 -1.4744662352033515 2216 17 -0.9761692626110516 2216 18 -0.7118994193369232 2216 19 -0.8738440508540393 2216 20 -0.7915391572629673 2216 21 -0.6997949509166332 2216 22 -1.2324363309247133 2216 23 -0.9007704030560149 2216 24 -0.9615136935122237 2216 25 -1.023751314008642 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3929.0 3 1.0 4 61.0 5 450.0 6 700.0 7 463.0 8 378.0 9 245.0 10 204.0 11 238.0 12 180.0 13 150.0 14 133.0 15 107.0 16 137.0 17 138.0 18 171.0 19 218.0 20 319.0 21 371.0 22 436.0 23 573.0 24 595.0 25 686.0 26 660.0 27 707.0 28 754.0 29 867.0 30 1075.0 31 1407.0 32 1766.0 33 2249.0 34 2949.0 35 4056.0 36 5958.0 37 9883.0 38 29339.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.67533525764083 18.85591172124242 12.634137583382824 24.83461543773392 2 11.993769470404985 23.21699335593968 36.09020483555261 28.69903233810272 3 19.14541695382495 30.986905582356993 28.00964851826327 21.85802894555479 4 11.455390001516175 19.94872572397348 30.046450083389615 38.54943419112073 5 10.8704648917092 39.97516898882604 34.14815836667127 15.006207752793488 6 31.638582219639744 32.01765308098835 18.975678592180625 17.368086107191278 7 22.936715841720666 32.91051978491659 23.154556735144077 20.99820763821867 8 29.706368899917283 31.00358422939068 21.662531017369727 17.627515853322304 9 28.01632045432616 13.082553379188663 19.151722331729776 39.7494038347554 10 17.129348844902687 30.58526768484314 30.269614599988977 22.015768870265205 11 28.87819938527697 25.727399280525958 18.914448747811942 26.479952586385124 12 24.180943586068114 30.420520171460865 28.022273372569018 17.376262869902003 13 31.964219260402736 20.0680881562444 23.465604454674512 24.502088128678345 14 22.23707787732598 26.024810475534117 26.02205375603032 25.71605789110958 15 33.15093793502681 21.963254448472153 23.629622482874588 21.256185133626452 16 19.250754620759995 26.557137540832223 32.369440271249985 21.8226675671578 17 23.371610537489143 28.79337202409672 28.35499924180808 19.480018196606057 18 25.442865217331367 21.24098760666676 32.14133087029046 21.17481630571141 19 21.6269485755241 28.69075021019117 32.872520777914076 16.80978043637065 20 23.318126059570247 23.482144087770322 31.14412911940237 22.055600733257066 21 20.82092843753446 25.922097254383065 32.804057779247984 20.452916528834493 22 21.068735958540653 26.85622923931471 25.865229556324344 26.209805245820295 23 17.800383189292752 26.054115149760854 31.15825166439234 24.987249996554052 24 21.563546648656846 31.644453020550493 27.68596749962097 19.106032831171696 25 21.449147519744187 22.856394635645668 26.478574283627143 29.21588356098301 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 414.0 1 414.0 2 339.0 3 264.0 4 264.0 5 264.0 6 449.5 7 635.0 8 635.0 9 635.0 10 602.0 11 569.0 12 569.0 13 569.0 14 459.0 15 349.0 16 349.0 17 349.0 18 593.5 19 838.0 20 838.0 21 838.0 22 1237.0 23 1636.0 24 1636.0 25 1636.0 26 2197.0 27 2758.0 28 2758.0 29 2758.0 30 3173.0 31 3588.0 32 3588.0 33 3588.0 34 3742.5 35 3897.0 36 3897.0 37 3897.0 38 4097.0 39 4297.0 40 4297.0 41 4297.0 42 4748.0 43 5199.0 44 5199.0 45 5199.0 46 11612.0 47 18025.0 48 18025.0 49 18025.0 50 16073.5 51 14122.0 52 14122.0 53 14122.0 54 10318.0 55 6514.0 56 6514.0 57 6514.0 58 5057.5 59 3601.0 60 3601.0 61 3601.0 62 3264.5 63 2928.0 64 2928.0 65 2928.0 66 2218.5 67 1509.0 68 1509.0 69 1509.0 70 1166.5 71 824.0 72 824.0 73 824.0 74 601.0 75 378.0 76 378.0 77 378.0 78 271.5 79 165.0 80 165.0 81 165.0 82 100.0 83 35.0 84 35.0 85 35.0 86 21.5 87 8.0 88 8.0 89 8.0 90 4.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20123220266563754 2 0.009648119305886731 3 0.004134908273951456 4 0.002756605515967637 5 0.08683307375298058 6 0.3735200474136149 7 0.031700963433627834 8 0.017917935853789643 9 0.008269816547902912 10 0.006891513789919094 11 0.0 12 0.0 13 0.0 14 0.004134908273951456 15 0.0 16 0.0 17 0.016539633095805823 18 0.019296238611773462 19 0.0 20 0.0 21 0.0013783027579838186 22 0.0 23 0.005513211031935274 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 72553.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.521287886097056 #Duplication Level Percentage of deduplicated Percentage of total 1 82.68621738028882 35.98610670819952 2 10.61565746136306 9.24014168952352 3 3.0972890803141624 4.043940291924524 4 1.250950088674943 2.1777183576144337 5 0.6080567519635166 1.323170647664466 6 0.31036230048137825 0.8104420216944854 7 0.2185203952368888 0.6657202321061845 8 0.180516848239169 0.6285060576406213 9 0.12034456549277932 0.471379543230466 >10 0.6935647327083861 5.726847959422767 >50 0.06650620724600963 2.068832439733712 >100 0.10450975424372941 11.94712830620374 >500 0.03166962249809982 10.585365181315728 >1k 0.01583481124904991 14.324700563725829 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 3381 4.660041624743291 No Hit GTACATGGGAAGCAGTGGTATCAAC 3199 4.409190522790237 No Hit GAGTACATGGGAAGCAGTGGTATCA 1390 1.915840833597508 No Hit GTATCAACGCAGAGTACTTTTTTTT 1295 1.7849020715890451 No Hit CATGTACTCTGCGTTGATACCACTG 1128 1.5547255110057474 No Hit TATCAACGCAGAGTACATGGGAAGC 975 1.3438451890342233 No Hit TATCAACGCAGAGTACTTTTTTTTT 958 1.3204140421484984 No Hit GCGTTGATACCACTGCTTCCCATGT 873 1.2032583077198737 No Hit GGTATCAACGCAGAGTACTTTTTTT 865 1.1922318856560032 No Hit GTATCAACGCAGAGTACATGGGAAG 820 1.1302082615467315 No Hit ACGCAGAGTACATGGGAAGCAGTGG 783 1.0792110595013302 No Hit ACTCTGCGTTGATACCACTGCTTCC 737 1.0158091326340744 No Hit GGTATCAACGCAGAGTACATGGGAA 631 0.8697090402877895 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 537 0.7401485810373106 No Hit CTTATACACATCTCCGAGCCCACGA 501 0.6905296817498932 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 486 0.6698551403801359 No Hit ATACCACTGCTTCCCATGTACTCTG 437 0.6023183052389288 No Hit GTACTCTGCGTTGATACCACTGCTT 410 0.5651041307733657 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 390 0.5375380756136893 No Hit GCTTCCCATGTACTCTGCGTTGATA 358 0.4934323873582071 No Hit CAGTGGTATCAACGCAGAGTACATG 353 0.486540873568288 No Hit TACCACTGCTTCCCATGTACTCTGC 345 0.4755144515044174 No Hit GTGGTATCAACGCAGAGTACATGGG 343 0.47275784598844983 No Hit GGGAAGCAGTGGTATCAACGCAGAG 336 0.46310972668256306 No Hit CTGCTTCCCATGTACTCTGCGTTGA 323 0.44519179082877347 No Hit GCAGAGTACATGGGAAGCAGTGGTA 308 0.42451724945901614 No Hit CATGGGAAGCAGTGGTATCAACGCA 301 0.41486913015312943 No Hit ATACACATCTCCGAGCCCACGAGAC 272 0.37489835017159867 No Hit ACATGGGAAGCAGTGGTATCAACGC 253 0.34871059776990615 No Hit CCACTGCTTCCCATGTACTCTGCGT 253 0.34871059776990615 No Hit GCAGTGGTATCAACGCAGAGTACAT 253 0.34871059776990615 No Hit GATACCACTGCTTCCCATGTACTCT 252 0.3473322950119223 No Hit ATGGGAAGCAGTGGTATCAACGCAG 239 0.32941435915813266 No Hit GTTGATACCACTGCTTCCCATGTAC 235 0.32390114812619736 No Hit AAGCAGTGGTATCAACGCAGAGTAC 233 0.3211445426102298 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 228 0.31425302882031064 No Hit TCTCCGAGCCCACGAGACCAGAGAG 217 0.2990916984824887 No Hit CCATGTACTCTGCGTTGATACCACT 215 0.29633509296652105 No Hit CTTCCCATGTACTCTGCGTTGATAC 201 0.27703885435474757 No Hit ACACATCTCCGAGCCCACGAGACCA 196 0.2701473405648285 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 193 0.266012432290877 No Hit ATCAACGCAGAGTACATGGGAAGCA 193 0.266012432290877 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 158 0.21777183576144338 No Hit TTCCCATGTACTCTGCGTTGATACC 156 0.21501523024547572 No Hit ATCAACGCAGAGTACTTTTTTTTTT 146 0.20123220266563754 No Hit TGATACCACTGCTTCCCATGTACTC 141 0.19434068887571843 No Hit GTGGTATCAACGCAGAGTACTTTTT 123 0.1695312392320097 No Hit GGAAGCAGTGGTATCAACGCAGAGT 121 0.16677463371604206 No Hit TCAACGCAGAGTACATGGGAAGCAG 91 0.1254255509765275 No Hit GTACTGGTTCACTATCGGTCAGTCA 89 0.12266894546055986 No Hit GTACATGGTAAGCAGTGGTATCAAC 86 0.11853403718660842 No Hit AAAGTACTCTGCGTTGATACCACTG 84 0.11577743167064077 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 84 0.11577743167064077 No Hit AAAAAGTACTCTGCGTTGATACCAC 83 0.11439912891265695 No Hit CATCTCCGAGCCCACGAGACCAGAG 82 0.11302082615467313 No Hit CTCTGCGTTGATACCACTGCTTCCC 78 0.10750761512273786 No Hit CTGCGTTGATACCACTGCTTCCCAT 78 0.10750761512273786 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0013783027579838188 0.0 0.0 0.0 0.0 9 0.0013783027579838188 0.0 0.0 0.0 0.0 10 0.0013783027579838188 0.0 0.0 0.0 0.0 11 0.0013783027579838188 0.0 0.0 0.0 0.0 12 0.0013783027579838188 0.0 0.0 0.0 0.0 13 0.0027566055159676376 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAC 50 2.0669904E-7 19.0311 1 CCAGAGA 25 0.0059930566 18.991726 18 TATACAC 55 5.6229874E-7 17.300999 3 TTATACA 60 1.3962272E-6 15.859249 2 ATACACA 80 2.7328239E-5 11.894437 4 TACACAT 85 5.0766823E-5 11.194763 5 CCATGTA 925 0.0 10.081339 2 TGCGTTG 980 0.0 10.077241 12 CTGCGTT 995 0.0 10.013853 11 AGTGGTA 810 0.0 9.964793 14 CGTTGAT 1005 0.0 9.92105 14 GTGGTAT 810 0.0 9.847561 15 CAGTGGT 820 0.0 9.843272 13 TGATACC 1010 0.0 9.777918 17 TTGATAC 1010 0.0 9.777918 16 TGGTATC 825 0.0 9.668514 16 TACTCTG 995 0.0 9.632373 7 TCTGCGT 1035 0.0 9.626843 10 CCCATGT 940 0.0 9.616778 1 GTTGATA 1040 0.0 9.587169 15 >>END_MODULE