FastQCFastQC Report
Thu 26 May 2016
SRR1513131_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513131_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065317
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC33610.16273530891383745No Hit
CCCATGTACTCTGCGTTGATACCAC28600.13847753153632106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCGC500.001514671313.282648
TAGGACT2900.012.1581654
ATAGGAC1650.012.1282923
CGCAGAT1604.7293724E-1111.2665258
TACCGTG700.001507797510.8432347
TAGGACC3650.010.4431484
GTAGGAC9400.010.3403833
GGCGAGG4100.010.18403419
ATAAGAC2350.010.13762953
CTGTAGG9800.010.1349911
TGTAGGA10650.010.1178682
GTCCTAC8550.010.0529271
GCGTTTA856.665643E-410.04715413
TGGCGAG7500.09.99554218
GACGTGG4500.09.9095127
GGACGTG8350.09.8850456
TTACCCT1752.4410838E-99.80164054
GTGTAGG2750.09.7239211
GTGTAAG2850.09.7178291
AGGACGT8350.09.6814825
Produced by FastQC (version 0.11.5)