##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513131_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2065317
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.223188982611386	31.0	30.0	34.0	23.0	34.0
2	29.08413768927482	31.0	30.0	34.0	18.0	34.0
3	29.277501226204016	31.0	30.0	34.0	22.0	34.0
4	32.33078263530489	35.0	33.0	37.0	19.0	37.0
5	32.47203262259498	37.0	33.0	37.0	22.0	37.0
6	32.82075971872599	37.0	35.0	37.0	26.0	37.0
7	32.76543019788246	37.0	35.0	37.0	25.0	37.0
8	32.644194571583924	37.0	35.0	37.0	25.0	37.0
9	33.85414539269274	39.0	34.0	39.0	22.0	39.0
10	33.42339021080057	38.0	33.0	39.0	17.0	39.0
11	33.87859345562933	39.0	34.0	39.0	23.0	39.0
12	33.86413562663746	39.0	34.0	39.0	23.0	39.0
13	33.89980956918478	39.0	35.0	39.0	23.0	39.0
14	34.11167922406101	39.0	33.0	41.0	13.0	41.0
15	34.529488693503225	39.0	34.0	41.0	17.0	41.0
16	34.36629098583898	39.0	34.0	41.0	17.0	41.0
17	33.97128866900336	39.0	33.0	41.0	11.0	41.0
18	33.69395206643823	39.0	33.0	41.0	11.0	41.0
19	34.325394600441484	39.0	34.0	41.0	18.0	41.0
20	34.53482588871345	39.0	34.0	41.0	18.0	41.0
21	34.5803036531438	39.0	34.0	41.0	18.0	41.0
22	34.26192782996509	39.0	34.0	41.0	10.0	41.0
23	33.80195195217006	39.0	33.0	41.0	8.0	41.0
24	34.04265059552601	39.0	34.0	41.0	2.0	41.0
25	33.674219502381476	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.152702719065907
1101	2	1.348745074520874
1101	3	1.249549510674239
1101	4	1.566575001531504
1101	5	1.400323567357404
1101	6	1.2115289923467714
1101	7	1.1097391614426755
1101	8	1.1488147640056567
1101	9	1.632630509461201
1101	10	1.9108163940401468
1101	11	1.4956518504400549
1101	12	1.4626101505776177
1101	13	1.393017101603732
1101	14	2.409549589182852
1101	15	2.010906064272163
1101	16	2.1194495804298015
1101	17	2.4404428510421496
1101	18	2.463678904424306
1101	19	1.995283397461904
1101	20	1.6024037574323984
1101	21	1.682481235201088
1101	22	1.8795239892714761
1101	23	2.1908604834200958
1101	24	1.8854458873806053
1101	25	1.9391828056050002
1102	1	0.8351743362833481
1102	2	1.117302604975766
1102	3	0.9831657764772714
1102	4	1.2719811605569475
1102	5	1.21924422859054
1102	6	0.8697364642198053
1102	7	0.9481881747604533
1102	8	1.014633528619143
1102	9	1.3152089298487084
1102	10	1.4355083916047775
1102	11	1.1685930434634386
1102	12	1.0584343384897963
1102	13	1.1024536413345132
1102	14	2.0226413554898386
1102	15	1.7034702612913932
1102	16	1.7667771988342267
1102	17	1.9874535134520386
1102	18	2.2372242068755384
1102	19	1.6206050860520023
1102	20	1.4550096268741584
1102	21	1.3390361358677438
1102	22	1.6026876142861823
1102	23	1.8604965340307302
1102	24	1.657516410728924
1102	25	2.0154223619177714
1103	1	0.7292117983755304
1103	2	0.9770815762999128
1103	3	0.7994375446536779
1103	4	1.2927422954647838
1103	5	1.1590494240152083
1103	6	0.842473681560783
1103	7	0.870116356340624
1103	8	0.8574045701738484
1103	9	1.2166174417635034
1103	10	1.554927687836411
1103	11	1.0737303807950482
1103	12	1.0641555171539423
1103	13	1.1343327772747784
1103	14	1.8953096736176462
1103	15	1.526285228744463
1103	16	1.5144366925628034
1103	17	1.8619423607894063
1103	18	2.0832605411406036
1103	19	1.6007057213027451
1103	20	1.2542085940219536
1103	21	1.3117894650247237
1103	22	1.559635569940525
1103	23	1.8440238981482295
1103	24	1.51195336105895
1103	25	1.7536940728548274
1104	1	0.8393179679794649
1104	2	1.0545638354833713
1104	3	0.816392589715587
1104	4	1.354940263732935
1104	5	1.2687065214167887
1104	6	0.9597518971018104
1104	7	0.7715092457510835
1104	8	0.8114567860913695
1104	9	1.2264429150223677
1104	10	1.3318399021913052
1104	11	0.9705336516179912
1104	12	0.8788069959092724
1104	13	0.8678663158473228
1104	14	1.901803706526188
1104	15	1.5810110005283207
1104	16	1.5464171985084008
1104	17	1.830982073055985
1104	18	2.0613137890222717
1104	19	1.3804824751567466
1104	20	1.1996631013403487
1104	21	1.1237668562004828
1104	22	1.4678187624537529
1104	23	1.7476247084078835
1104	24	1.570084090151063
1104	25	1.847989377155784
1105	1	0.832215717621203
1105	2	1.0323986758178272
1105	3	0.9100289104034509
1105	4	1.2620922585602514
1105	5	1.1635622763516338
1105	6	0.9604583858988036
1105	7	1.0510984818014038
1105	8	1.0583489918957554
1105	9	1.2867902740727573
1105	10	1.5848493499350695
1105	11	1.1130974092985113
1105	12	1.201760687236039
1105	13	1.0901397955609369
1105	14	1.777542269852809
1105	15	1.4916238023646997
1105	16	1.6175879165239522
1105	17	2.010166529314354
1105	18	1.5752482615687882
1105	19	1.4009351405617423
1105	20	1.27445056029962
1105	21	1.2384178220182207
1105	22	1.4442835456237617
1105	23	1.7692624717162246
1105	24	1.5937656687335817
1105	25	1.8454150777237217
1106	1	0.691037312241761
1106	2	0.8287743658594557
1106	3	0.7134050487319819
1106	4	1.229353584208667
1106	5	1.101690076543754
1106	6	0.7551356065199997
1106	7	0.7372745945135435
1106	8	0.7679533466828232
1106	9	0.8543497745530644
1106	10	0.8376020108380473
1106	11	0.798511587972861
1106	12	0.7696918303676412
1106	13	0.652032744648352
1106	14	1.672284812279969
1106	15	1.2464460117787226
1106	16	1.277076562057097
1106	17	1.6238968142967423
1106	18	1.747186935920375
1106	19	1.2996353134724004
1106	20	1.0598192954901435
1106	21	0.9617073769333686
1106	22	1.13723191578066
1106	23	1.6770126094043505
1106	24	1.2800415829186562
1106	25	1.5868012832952871
1107	1	0.7434873565175373
1107	2	0.9222290401162567
1107	3	0.9221204440514015
1107	4	1.202790244550222
1107	5	1.124872467435594
1107	6	0.865626991719239
1107	7	0.7686370233560496
1107	8	0.8859945864045145
1107	9	1.1554357707579967
1107	10	1.4324506382890263
1107	11	1.038279734715431
1107	12	0.9534381175276678
1107	13	0.8280751481904147
1107	14	1.777976703488065
1107	15	1.34186997129801
1107	16	1.3952885291665211
1107	17	1.852414866162654
1107	18	2.1206350320620544
1107	19	1.528557464010035
1107	20	1.2606641278747048
1107	21	1.0896060739605673
1107	22	1.364283958791411
1107	23	1.6804022615232626
1107	24	1.593746225549907
1107	25	1.843587722257972
1108	1	0.505552415819917
1108	2	0.7768217732668639
1108	3	0.5766449231827053
1108	4	1.0215984555206603
1108	5	1.0519663351752726
1108	6	0.5240299191377034
1108	7	0.5816889071312445
1108	8	0.5452223987857678
1108	9	0.8251262215084978
1108	10	0.9779253253389868
1108	11	0.6735048836414066
1108	12	0.5062873357034903
1108	13	0.5073831024637911
1108	14	1.5334176685260417
1108	15	1.0868250701591364
1108	16	1.0290485394582234
1108	17	1.4342327339452083
1108	18	1.6716742679982133
1108	19	1.0458812393983266
1108	20	0.9165519006376641
1108	21	0.8981213882328021
1108	22	1.150688186967102
1108	23	1.4113384851105621
1108	24	1.0612124052664313
1108	25	1.379063731506207
1109	1	0.7259244278070618
1109	2	0.9464578985872976
1109	3	0.9082328345133774
1109	4	1.1014932898146768
1109	5	1.212020825056129
1109	6	0.8179031104348127
1109	7	0.7361947323149849
1109	8	0.8178154199681344
1109	9	0.9494336705756155
1109	10	1.4012672285016876
1109	11	0.9813015409950978
1109	12	0.8956124817926323
1109	13	0.7954455601823298
1109	14	1.7451168323238093
1109	15	1.2306401364263877
1109	16	1.4793388107260554
1109	17	1.8077706382867262
1109	18	1.9008957740036792
1109	19	1.2375563156646976
1109	20	1.1218371595678107
1109	21	1.0764369478697944
1109	22	1.2918338074186195
1109	23	1.6418497907385188
1109	24	1.291049763313275
1109	25	1.6481448723964434
1110	1	0.4246753409379558
1110	2	0.6373494875063663
1110	3	0.5504977000108333
1110	4	0.897370851831873
1110	5	0.6467029773547353
1110	6	0.395643055054002
1110	7	0.39715731441373237
1110	8	0.4339930222910553
1110	9	0.6428017271419577
1110	10	0.9173074631150229
1110	11	0.5817971275860856
1110	12	0.36366652780994
1110	13	0.26808484695652623
1110	14	1.0916169644945626
1110	15	0.7659548327394177
1110	16	0.9039053580645842
1110	17	1.3296132958937505
1110	18	0.8794581274362798
1110	19	0.7509596802846303
1110	20	0.5038666067814717
1110	21	0.24360002821222082
1110	22	0.6676902064855881
1110	23	-0.4747032234091293
1110	24	0.20992525104092863
1110	25	0.6581173656408907
1111	1	0.4049671221743196
1111	2	0.6995799084713497
1111	3	0.7146748464021222
1111	4	1.0508933512227685
1111	5	1.020438917745473
1111	6	0.5911937794200597
1111	7	0.6160508054270935
1111	8	0.686397981875956
1111	9	0.9289818775573551
1111	10	1.1663081297834808
1111	11	0.7830520171574094
1111	12	0.6303459566705243
1111	13	0.4681670426250548
1111	14	1.4642056461242348
1111	15	1.1153051656720407
1111	16	1.1743598974341651
1111	17	1.5927987394960113
1111	18	1.7665207434004486
1111	19	1.182493633521453
1111	20	0.8156130907077994
1111	21	0.6361646669395
1111	22	1.0197661156580367
1111	23	1.3026723518936194
1111	24	1.1199919446599225
1111	25	1.3570081257232474
1112	1	0.30735632397740176
1112	2	0.38917965307378566
1112	3	0.3143370808505743
1112	4	0.8120519412191669
1112	5	0.6358720654257795
1112	6	0.42650627977509714
1112	7	0.20621591631712732
1112	8	0.3725962126494693
1112	9	0.5667000154424997
1112	10	0.8178616278856055
1112	11	0.2163587528894766
1112	12	0.27792723493836746
1112	13	0.245216784178929
1112	14	0.7973231816437689
1112	15	0.6712457152994133
1112	16	0.5367868029465299
1112	17	1.0878875246117516
1112	18	1.1278210063342087
1112	19	0.6736921880863633
1112	20	0.5026607531800735
1112	21	0.44876121263442315
1112	22	0.6739500874915905
1112	23	1.0717185760504222
1112	24	0.8032169751360811
1112	25	1.0853266940426636
1113	1	0.13928081425976657
1113	2	0.15938642422642246
1113	3	0.19098860663325468
1113	4	0.4567183032400237
1113	5	0.5652447986797426
1113	6	0.20477673660382578
1113	7	0.22052327504754032
1113	8	0.3132905711472702
1113	9	0.4487115752360822
1113	10	0.7089571427846479
1113	11	0.2928695637567813
1113	12	0.11404143608015715
1113	13	0.0028556692059424904
1113	14	0.6522556536335813
1113	15	0.20666596831395623
1113	16	0.34320072225579423
1113	17	0.647996361021363
1113	18	0.5597591308908818
1113	19	0.4538578282193768
1113	20	0.26656095336036856
1113	21	0.12097389043849205
1113	22	0.3088346726884481
1113	23	0.3204080269765299
1113	24	0.2764727036886043
1113	25	0.6764497651332277
1114	1	-0.844821810299841
1114	2	-0.5820228504794258
1114	3	-0.555904724339122
1114	4	-0.3342951903752933
1114	5	-3.328391375801175
1114	6	-1.1280939244936157
1114	7	-0.7902533933835869
1114	8	-0.5535304657226234
1114	9	-0.215559816974924
1114	10	-0.02981390444183063
1114	11	-0.5048580943266856
1114	12	-0.5908508625219184
1114	13	-0.6253496456693455
1114	14	-6.005994643090684
1114	15	-2.4256028838339887
1114	16	-1.1191492775798153
1114	17	-0.2150831554460666
1114	18	-6.3729936389661646
1114	19	-2.356141509523056
1114	20	-1.3493817937685577
1114	21	-1.093059582219432
1114	22	-0.853993984370625
1114	23	-0.3056405827287847
1114	24	-0.4607840751751411
1114	25	-0.34287472275615016
1115	1	-0.8620356455047187
1115	2	-0.5120410332015766
1115	3	-0.5932173925825701
1115	4	-3.034985476824197
1115	5	-1.0871607215783072
1115	6	-0.6955862714142711
1115	7	-0.5789484101812903
1115	8	-0.45450627773032437
1115	9	-0.22249961511829497
1115	10	-0.64844897507745
1115	11	-0.724196768834652
1115	12	-0.7623422541393978
1115	13	-0.7417399314079844
1115	14	-6.1490896478139305
1115	15	-2.544976805122687
1115	16	-1.2841029684593366
1115	17	-0.39248384298024774
1115	18	-0.17902376830497957
1115	19	-0.5126737945088209
1115	20	-0.5332035214112665
1115	21	-0.8437770362121668
1115	22	-1.9230664879752126
1115	23	-1.0680370619571562
1115	24	-0.8037569147339241
1115	25	-0.28500021905212947
1116	1	-0.4045535236715523
1116	2	-0.15591634058580084
1116	3	-0.2463620438658225
1116	4	-0.015784698932343133
1116	5	-0.7214318823642074
1116	6	-0.4592552068771312
1116	7	-0.4353704941439318
1116	8	-0.3879144833199959
1116	9	-0.3253573799642453
1116	10	-0.21413909808399012
1116	11	-0.5256159887228904
1116	12	-0.7808633511690886
1116	13	-0.8175930588979483
1116	14	0.1423454483621498
1116	15	-0.3504330650577572
1116	16	-0.38074202505239185
1116	17	0.07181449512984273
1116	18	-0.17417565344073438
1116	19	-0.3153106589452719
1116	20	-0.7051106679210619
1116	21	-0.7998119250649438
1116	22	-0.8012311745655296
1116	23	-1.4937198645613137
1116	24	-1.1127837703039702
1116	25	-0.4650670870959317
1201	1	0.6453762975118096
1201	2	0.921787524615592
1201	3	0.8584038048694609
1201	4	1.1250985269255267
1201	5	1.033585592092038
1201	6	0.7452522677311322
1201	7	0.7374448072795019
1201	8	0.843794917877581
1201	9	0.8968731133419325
1201	10	1.415300996108094
1201	11	0.7925551886275244
1201	12	0.8836971986164173
1201	13	0.7310151341737168
1201	14	1.5515536829837302
1201	15	1.2753030985217677
1201	16	1.4298076913533322
1201	17	1.694669711187089
1201	18	1.2703434661986037
1201	19	1.156780190722813
1201	20	1.073655760301925
1201	21	1.1181182443638136
1201	22	1.2649826195449805
1201	23	1.603484360631846
1201	24	1.2187031868466462
1201	25	1.5523596466340734
1202	1	0.6800793884993723
1202	2	0.8617245228604169
1202	3	0.870442100958833
1202	4	1.222139793756888
1202	5	1.0451228737241465
1202	6	0.778027653940697
1202	7	0.6309445720848359
1202	8	0.8793955767372879
1202	9	1.0398747216853934
1202	10	1.3775913988547046
1202	11	0.8856320749962521
1202	12	0.7792086775320115
1202	13	0.868443074670779
1202	14	1.7448378657884547
1202	15	1.3237867615384147
1202	16	1.4567734284460698
1202	17	1.8352523541878085
1202	18	2.05191972669256
1202	19	1.5936816832256895
1202	20	1.2798086835068005
1202	21	1.1851400715825235
1202	22	1.3928693653160664
1202	23	1.6338189663412237
1202	24	1.3462128869878711
1202	25	1.7567153364833672
1203	1	0.7761754059412773
1203	2	0.9065710702213288
1203	3	0.8170705737723694
1203	4	1.1678489638532525
1203	5	1.0359075886600735
1203	6	0.766232730914183
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2211	15	-0.6808573675527754
2211	16	-6.778229564897828
2211	17	-2.367449938233154
2211	18	-1.0916402213933338
2211	19	-1.092709513267863
2211	20	-1.1726106094469912
2211	21	-1.0966602894266444
2211	22	-1.0092606693159354
2211	23	-0.6601691630580859
2211	24	-0.9734701496787039
2211	25	-8.015854294748259
2212	1	-1.323899399673774
2212	2	-3.812335257066639
2212	3	-1.596806233319299
2212	4	-3.4717244248411063
2212	5	-1.216727299021855
2212	6	-1.0464296423487767
2212	7	-0.9570964462104392
2212	8	-0.8764001978119005
2212	9	-0.9826365301226545
2212	10	-0.3841406135963439
2212	11	-0.8777763154962201
2212	12	-0.9758130866760979
2212	13	-1.1952645102536081
2212	14	-6.812574554235873
2212	15	-5.231554832866831
2212	16	-2.8781606023471262
2212	17	-7.091466805612757
2212	18	-2.893046668906173
2212	19	-1.7284609761203598
2212	20	-2.021893163031727
2212	21	-1.558907525027415
2212	22	-1.4041294115194916
2212	23	-0.9145863046228015
2212	24	-1.3960460188912975
2212	25	-0.9956522913653458
2213	1	-0.34626918095752757
2213	2	-0.20273229292233097
2213	3	-0.2606449571816789
2213	4	0.014773430453494996
2213	5	-0.19329930252188632
2213	6	-0.3906836247041241
2213	7	-0.44922806663370807
2213	8	-0.5068454705850698
2213	9	-0.1794322715734893
2213	10	-3.9098749377131092
2213	11	-1.6439840714002898
2213	12	-1.2171823978996557
2213	13	-0.9908617319926876
2213	14	-0.0878856903811851
2213	15	-0.5892121313318626
2213	16	-0.3769977608287718
2213	17	0.04918920932927051
2213	18	0.21759961480860568
2213	19	-0.32770879060802116
2213	20	-0.7640148381585874
2213	21	-0.6807892286254713
2213	22	-0.992723093089829
2213	23	-1.267066964812095
2213	24	-0.7133650167265202
2213	25	-0.4157528503534422
2214	1	-1.039747038157767
2214	2	-0.6916983893935793
2214	3	-0.6962287600871946
2214	4	-0.24884877151787776
2214	5	-3.4152716754538766
2214	6	-1.4808294955812116
2214	7	-1.1355114368566852
2214	8	-0.9041423029176521
2214	9	-0.7763052594806723
2214	10	-4.3201330823848
2214	11	-1.92192139219879
2214	12	-1.4414896079277142
2214	13	-1.1660958851237666
2214	14	-0.5219496002681225
2214	15	-0.71823598881317
2214	16	-0.7572394841104071
2214	17	-0.3449343902016295
2214	18	-0.0221267781962311
2214	19	-0.7766117181937702
2214	20	-1.0456163008401305
2214	21	-1.183357269411701
2214	22	-0.8672441067112047
2214	23	-0.6491882310810055
2214	24	-1.068452243657248
2214	25	-0.8780290688799113
2215	1	-2.336991108505849
2215	2	-4.6400031942680435
2215	3	-2.9571774375038906
2215	4	-1.9965488904363617
2215	5	-2.056229784366124
2215	6	-2.236274280469278
2215	7	-2.339148471264494
2215	8	-2.5440179080259604
2215	9	-8.187784053322911
2215	10	-4.039393271688759
2215	11	-3.317570057926879
2215	12	-3.5809947880038457
2215	13	-3.111791120897866
2215	14	-8.247217801455182
2215	15	-5.047239621371091
2215	16	-9.534561942724128
2215	17	-4.822991670200501
2215	18	-3.388654779524426
2215	19	-3.9761037998280884
2215	20	-3.7146897511630037
2215	21	-3.8076856569698485
2215	22	-9.376046878771646
2215	23	-10.742778801780815
2215	24	-6.962187382598582
2215	25	-5.484799249883501
2216	1	-2.02780311021516
2216	2	-2.063603977664723
2216	3	-1.7391573970712422
2216	4	-7.0232704068975025
2216	5	-3.545935582155522
2216	6	-2.7313149104338628
2216	7	-2.255090052512582
2216	8	-7.953329143589553
2216	9	-4.133285667359107
2216	10	-2.373498200390415
2216	11	-2.2860588622772795
2216	12	-2.483371249120964
2216	13	-2.3465130252238
2216	14	-1.517140690777289
2216	15	-2.2009530321345636
2216	16	-1.8035546330512986
2216	17	-1.6529947032129826
2216	18	-7.666348494874512
2216	19	-4.787771201258003
2216	20	-4.2300357795515495
2216	21	-3.4857437848231605
2216	22	-3.107934776826344
2216	23	-2.301942276643455
2216	24	-2.6712690563253645
2216	25	-2.050478627766413
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	103782.0
3	1.0
4	894.0
5	5300.0
6	6321.0
7	5633.0
8	5277.0
9	4893.0
10	4562.0
11	4348.0
12	4165.0
13	4161.0
14	4174.0
15	4340.0
16	4388.0
17	4774.0
18	5190.0
19	6466.0
20	8126.0
21	10417.0
22	12856.0
23	15466.0
24	17561.0
25	20369.0
26	22420.0
27	25541.0
28	28436.0
29	33742.0
30	40343.0
31	48026.0
32	61188.0
33	79218.0
34	103628.0
35	137841.0
36	193936.0
37	278107.0
38	749427.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.4332143830574	17.89390113903215	12.165761525874352	25.507122952036106
2	14.77483617630968	19.734959749785073	40.573032186094196	24.91717188781105
3	18.502210983507798	24.53664980050419	29.44848473962629	27.512654476361725
4	11.734132753646593	16.37976615691137	38.282284029315754	33.60381706012628
5	13.141852729931088	37.63147726368072	35.81933854096887	13.40733146541932
6	32.69075056611595	36.29016323897745	17.864081807386917	13.155004387519686
7	28.514097650601418	31.172026108087636	22.12698200541029	18.186894235900656
8	25.805014445562552	35.659619349983586	20.429357179730303	18.10600902472356
9	26.674892842597387	14.813509072603589	19.622819502429355	38.88877858236967
10	14.24900577069479	28.250107994707776	35.11116190682817	22.389724327769265
11	34.924994237031385	22.226752959453883	23.830311084077355	19.017941719437378
12	23.379177041112108	24.393624662129273	31.10386682481188	21.12333147194674
13	29.068194531554592	21.245716804172893	25.452947201968275	24.233141462304246
14	21.083428257045664	20.825729750253778	28.173503111173105	29.917338881527456
15	24.010439574279278	29.089366088434122	23.056433377961802	23.8437609593248
16	23.065736132426302	27.28568405259898	26.658198165825386	22.990381649149334
17	22.3439224124151	27.570970338888635	27.50838720552747	22.576720043168798
18	22.427237696326962	26.84775469954491	28.492280599108426	22.232727005019694
19	23.858690861681016	26.780506185168573	26.94875671863997	22.41204623451044
20	24.22874567556585	26.539663228106846	26.812048698104974	22.419542398222326
21	23.836571476729976	26.304738572775765	26.53998892768558	23.31870102280868
22	23.84915673692527	26.67769800830354	26.422129982208087	23.051015272563106
23	23.080901985874203	26.57530053522659	27.011434408226147	23.33236307067306
24	23.578084891278493	26.644773970588737	26.766218849776024	23.010922288356745
25	23.503093640382104	26.23350857665418	26.883332897404678	23.38006488555904
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	143.0
1	143.0
2	272.0
3	401.0
4	401.0
5	401.0
6	812.0
7	1223.0
8	1223.0
9	1223.0
10	1976.5
11	2730.0
12	2730.0
13	2730.0
14	5019.0
15	7308.0
16	7308.0
17	7308.0
18	13770.5
19	20233.0
20	20233.0
21	20233.0
22	31972.0
23	43711.0
24	43711.0
25	43711.0
26	62687.0
27	81663.0
28	81663.0
29	81663.0
30	105288.0
31	128913.0
32	128913.0
33	128913.0
34	151377.0
35	173841.0
36	173841.0
37	173841.0
38	195658.5
39	217476.0
40	217476.0
41	217476.0
42	234689.0
43	251902.0
44	251902.0
45	251902.0
46	266496.0
47	281090.0
48	281090.0
49	281090.0
50	274509.5
51	267929.0
52	267929.0
53	267929.0
54	243336.0
55	218743.0
56	218743.0
57	218743.0
58	189860.5
59	160978.0
60	160978.0
61	160978.0
62	132377.5
63	103777.0
64	103777.0
65	103777.0
66	80836.0
67	57895.0
68	57895.0
69	57895.0
70	42882.5
71	27870.0
72	27870.0
73	27870.0
74	19478.0
75	11086.0
76	11086.0
77	11086.0
78	7750.0
79	4414.0
80	4414.0
81	4414.0
82	2919.5
83	1425.0
84	1425.0
85	1425.0
86	915.5
87	406.0
88	406.0
89	406.0
90	263.5
91	121.0
92	121.0
93	121.0
94	75.0
95	29.0
96	29.0
97	29.0
98	19.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1641878704334492
2	0.25797492588304843
3	0.26020218687978647
4	0.4596873022398014
5	0.25492454669186376
6	0.01786650669122464
7	0.01757599438730229
8	0.01563924569448661
9	0.01762441310462268
10	0.019415905645477183
11	0.01980325538404032
12	0.026533457091574803
13	0.027211319134060292
14	0.02425777737751638
15	0.04188219048213906
16	0.01985167410136071
17	0.04178535304749828
18	0.03142374754093439
19	0.026097688635691278
20	0.028857555522953618
21	0.03428045186283752
22	0.04338317071907121
23	0.028276530915108913
24	0.04870922962431433
25	0.036749806446177515
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2065317.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.21595813446842
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.32311957650583	61.839051541232635
2	11.333813954772937	16.822997239425646
3	2.8418302714650614	6.327274693569482
4	1.0718147362601191	3.1818303037674927
5	0.5102505771176342	1.893436773472535
6	0.25922904683240605	1.1543359252171204
7	0.1690399178379129	0.8781821625718768
8	0.10191632944175737	0.6051054431254527
9	0.07487238012469044	0.5001052885885828
>10	0.2926697546835126	3.601340470316476
>50	0.01187311721581673	0.5919611420931477
>100	0.007591584414328545	1.1194294584778823
>500	0.0013191688853650698	0.6509595920280208
>1k	6.595844426823072E-4	0.8339899661135833
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3361	0.16273530891383745	No Hit
CCCATGTACTCTGCGTTGATACCAC	2860	0.13847753153632106	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.841871732039198E-5
2	0.0	0.0	0.0	0.0	4.841871732039198E-5
3	0.0	0.0	0.0	0.0	4.841871732039198E-5
4	0.0	0.0	0.0	0.0	4.841871732039198E-5
5	0.0	9.683743464078396E-5	0.0	0.0	4.841871732039198E-5
6	0.0	9.683743464078396E-5	0.0	0.0	4.841871732039198E-5
7	0.0	9.683743464078396E-5	0.0	0.0	4.841871732039198E-5
8	0.0	9.683743464078396E-5	0.0	0.0	4.841871732039198E-5
9	0.0	9.683743464078396E-5	0.0	0.0	4.841871732039198E-5
10	0.0	9.683743464078396E-5	0.0	4.841871732039198E-5	4.841871732039198E-5
11	0.0	9.683743464078396E-5	0.0	4.841871732039198E-5	4.841871732039198E-5
12	4.841871732039198E-5	9.683743464078396E-5	0.0	4.841871732039198E-5	4.841871732039198E-5
13	4.841871732039198E-5	9.683743464078396E-5	0.0	4.841871732039198E-5	4.841871732039198E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGCGC	50	0.0015146713	13.28264	8
TAGGACT	290	0.0	12.158165	4
ATAGGAC	165	0.0	12.128292	3
CGCAGAT	160	4.7293724E-11	11.266525	8
TACCGTG	70	0.0015077975	10.843234	7
TAGGACC	365	0.0	10.443148	4
GTAGGAC	940	0.0	10.340383	3
GGCGAGG	410	0.0	10.184034	19
ATAAGAC	235	0.0	10.1376295	3
CTGTAGG	980	0.0	10.134991	1
TGTAGGA	1065	0.0	10.117868	2
GTCCTAC	855	0.0	10.052927	1
GCGTTTA	85	6.665643E-4	10.047154	13
TGGCGAG	750	0.0	9.995542	18
GACGTGG	450	0.0	9.909512	7
GGACGTG	835	0.0	9.885045	6
TTACCCT	175	2.4410838E-9	9.8016405	4
GTGTAGG	275	0.0	9.723921	1
GTGTAAG	285	0.0	9.717829	1
AGGACGT	835	0.0	9.681482	5
>>END_MODULE