FastQCFastQC Report
Thu 26 May 2016
SRR1513131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513131_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065317
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC38940.18854248524560635No Hit
CCCATGTACTCTGCGTTGATACCAC36850.17842297332564444No Hit
GTATCAACGCAGAGTACTTTTTTTT30540.1478707626964771No Hit
TATCAACGCAGAGTACTTTTTTTTT21040.10187298124210473No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCG602.5235016E-514.2770655
CTTAGAC2050.012.535963
TACGCTG802.8122771E-511.8975555
GGCGAGG4300.011.47480219
CGTGCGC1105.045549E-711.21428210
GTCTAGG1752.0008883E-1110.8825221
GCGTGCG700.001505628910.8451069
TGTGCGA700.001506237310.84458110
AGCGTGC803.8081067E-410.6764268
TACCGTA909.601659E-510.5464077
GTTCTAA2300.010.35022451
TTAGACT2053.6379788E-1210.2144864
GTATAGT1701.4970283E-910.0823361
CGAAATC856.6432374E-410.05083613
TAGGACC3050.09.9861454
AGGACGT8900.09.9458215
TTATACC1951.5825208E-109.7620954
CAGGACT4650.09.6203894
GTCCTAA6400.09.5222061
GCTATAG1201.6543328E-59.5222061
Produced by FastQC (version 0.11.5)