FastQCFastQC Report
Thu 26 May 2016
SRR1513130_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513130_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856120
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC25020.1347973191388488No Hit
GTATCAACGCAGAGTACTTTTTTTT21030.11330086416826497No Hit
CCCATGTACTCTGCGTTGATACCAC20940.1128159817253195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA2050.013.5098351
ACTGCGT500.001514354713.2828918
TGGCGAG7650.011.16450118
GTCCTAC9050.010.9746491
TAGGACT2600.010.6283464
GTCCTAA4950.010.4182431
TTAGACT1752.237357E-1010.3456224
CTAACAC1502.3332177E-810.1638453
TATGGCG8500.010.15996916
TAGGACC4050.010.11706354
GAGTACG856.6644506E-410.04723811
CTTATAC2002.3646862E-1110.0050343
TTAGGAC4600.09.9428913
GGCGAGA5250.09.94172219
GTATTAG1252.4029996E-69.9320581
ATGGCGA8550.09.87856517
GCGCCAC1252.6072303E-69.86888713
TTTAGAC1553.8782673E-89.8359793
GTCCTAT1855.8935257E-109.8081661
TCCTACA10700.09.8023932
Produced by FastQC (version 0.11.5)