##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513130_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1856120
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.935200849083035	31.0	30.0	34.0	22.0	34.0
2	28.82072872443592	31.0	30.0	34.0	16.0	34.0
3	29.010694890416566	31.0	30.0	34.0	18.0	34.0
4	32.05418561299916	35.0	33.0	37.0	18.0	37.0
5	32.18022811025149	35.0	33.0	37.0	19.0	37.0
6	32.51719878025128	37.0	33.0	37.0	23.0	37.0
7	32.45859750447169	37.0	33.0	37.0	23.0	37.0
8	32.33733971941469	37.0	33.0	37.0	22.0	37.0
9	33.52429530418292	39.0	34.0	39.0	17.0	39.0
10	33.094350580781416	38.0	33.0	39.0	17.0	39.0
11	33.527629140357305	38.0	34.0	39.0	18.0	39.0
12	33.51272816412732	38.0	34.0	39.0	18.0	39.0
13	33.549170312264295	38.0	34.0	39.0	18.0	39.0
14	33.746026657759195	39.0	33.0	41.0	10.0	41.0
15	34.14815744671681	39.0	33.0	41.0	17.0	41.0
16	33.98317942805422	39.0	33.0	41.0	11.0	41.0
17	33.59207971467362	39.0	33.0	41.0	10.0	41.0
18	33.30570760511174	38.0	32.0	41.0	10.0	41.0
19	33.93198715600285	39.0	33.0	41.0	11.0	41.0
20	34.13876365752214	39.0	34.0	41.0	11.0	41.0
21	34.18111760015516	39.0	34.0	41.0	10.0	41.0
22	33.866748917095876	39.0	34.0	41.0	2.0	41.0
23	33.393138913432324	39.0	33.0	41.0	2.0	41.0
24	33.61874447772773	39.0	33.0	41.0	2.0	41.0
25	33.24778031592785	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7522799452526883
1101	2	1.0298060814410697
1101	3	0.9072772858293803
1101	4	1.231230252285286
1101	5	1.0987793490395816
1101	6	0.920454318300763
1101	7	0.7590330970507182
1101	8	0.8941552885167496
1101	9	1.338898388926907
1101	10	1.5820478681144152
1101	11	1.121992455930794
1101	12	1.1178816469476516
1101	13	0.9434573513391129
1101	14	1.9675164361029829
1101	15	1.5576063489616772
1101	16	1.6448638176465238
1101	17	1.9912334706489219
1101	18	1.9829191715546557
1101	19	1.461886513901682
1101	20	1.1873640588537029
1101	21	1.273602461148819
1101	22	1.6072376224061102
1101	23	1.798057457036414
1101	24	1.5450123558747393
1101	25	1.7074811821444555
1102	1	1.0198611861082973
1102	2	1.2980279441588998
1102	3	1.3003796244234778
1102	4	1.4788771269461733
1102	5	1.442346999132326
1102	6	1.0107425856655894
1102	7	1.151950084381724
1102	8	1.1435314430642762
1102	9	1.5299309301725543
1102	10	1.8727456295551121
1102	11	1.451131021591415
1102	12	1.321113444543073
1102	13	1.2924598098003486
1102	14	2.317594331031515
1102	15	1.845898784858953
1102	16	1.9298038404225224
1102	17	2.2916312399477192
1102	18	2.4669631220062485
1102	19	1.8543458607318115
1102	20	1.7779691446628547
1102	21	1.6482930330560066
1102	22	1.8394250043008071
1102	23	2.075607876696118
1102	24	1.9980693420332685
1102	25	2.3533324196697265
1103	1	0.7421847273597351
1103	2	0.9885945561705647
1103	3	0.7634393881960477
1103	4	1.3312011100194852
1103	5	1.1868160028730799
1103	6	0.8943838852110062
1103	7	0.8664074780253799
1103	8	0.9510280557632242
1103	9	1.270442314449582
1103	10	1.542729846695842
1103	11	1.2054468849604518
1103	12	1.0597002036323317
1103	13	0.9726828807569419
1103	14	1.8050098939616817
1103	15	1.372186204717373
1103	16	1.3935202005957166
1103	17	1.8134460723990458
1103	18	2.110205947309879
1103	19	1.4979168615074556
1103	20	1.2073113946162195
1103	21	1.1728854523744232
1103	22	1.4291050270791814
1103	23	2.0705981075235087
1103	24	1.5652969588460621
1103	25	1.8720988038976714
1104	1	0.8315702022200675
1104	2	1.081122489131456
1104	3	0.9425594999278921
1104	4	1.3577374228686452
1104	5	1.3093718687948694
1104	6	0.9949425444288806
1104	7	0.9488097126622463
1104	8	0.9573129719643418
1104	9	1.2847579569076828
1104	10	1.4271572209975147
1104	11	1.1010474436196702
1104	12	1.0816974103362398
1104	13	1.0428644450027562
1104	14	2.0372026313918496
1104	15	1.5160409533207257
1104	16	1.6924028821599677
1104	17	2.009325960667198
1104	18	2.2516165618350215
1104	19	1.5845090402784052
1104	20	1.3539594393089516
1104	21	1.4797988601956504
1104	22	1.7674430159059966
1104	23	2.041966822607307
1104	24	1.695883551024835
1104	25	1.9782440552943186
1105	1	0.7925417581580199
1105	2	1.0983224490721781
1105	3	1.01188487106824
1105	4	1.2878941671345459
1105	5	1.1953405633556855
1105	6	0.806769997467768
1105	7	0.8402900115873564
1105	8	0.8513641935750371
1105	9	1.171353071056899
1105	10	1.4475901670911426
1105	11	0.9374377226335469
1105	12	0.8624625179141532
1105	13	0.862460660288825
1105	14	1.719490361609374
1105	15	1.4127615191235208
1105	16	1.5615469258581314
1105	17	1.83072496688132
1105	18	1.3270374062767445
1105	19	1.238632186056691
1105	20	1.0972183061246739
1105	21	1.1154320829548467
1105	22	1.1077733618958234
1105	23	1.423241625320287
1105	24	1.2423096416535273
1105	25	1.5819766230941568
1106	1	0.4215917115732317
1106	2	0.5538058539339801
1106	3	0.4593944618728827
1106	4	0.9718790926173497
1106	5	1.0320403911704332
1106	6	0.5603981339408861
1106	7	0.49288442116671405
1106	8	0.40719836896276007
1106	9	0.6952457131220413
1106	10	0.5037903745996033
1106	11	0.4286482747955844
1106	12	0.28883418451723486
1106	13	0.2642913132020013
1106	14	1.219207283641083
1106	15	0.8456820099068736
1106	16	0.7948706181302256
1106	17	1.2805720432397578
1106	18	1.3169940461166476
1106	19	0.6984258837564994
1106	20	0.5560712003507433
1106	21	0.44150442537580403
1106	22	0.7738113903439299
1106	23	1.1017457921049
1106	24	0.7064041552332725
1106	25	0.8739572704073169
1107	1	0.6154830979859689
1107	2	0.7305783936287327
1107	3	0.6945468963174619
1107	4	1.0192952851208688
1107	5	0.930610024455774
1107	6	0.6092997730248726
1107	7	0.6269006959847161
1107	8	0.7040192470408542
1107	9	1.067787606184055
1107	10	1.1711513406833518
1107	11	0.7522708201693007
1107	12	0.7751599666574336
1107	13	0.8642544304537267
1107	14	1.5358072071887605
1107	15	0.9135892559013712
1107	16	1.183879314886859
1107	17	1.6354949741725378
1107	18	1.7935626315411213
1107	19	1.293800152055283
1107	20	1.0516070443977839
1107	21	1.0031634651297878
1107	22	1.2426708707368448
1107	23	1.5538711299423227
1107	24	1.2452388802186434
1107	25	1.6082436930372026
1108	1	0.7035306944117785
1108	2	0.9455905414712404
1108	3	0.7616154904038446
1108	4	1.1819113584133163
1108	5	1.164119023842595
1108	6	0.7107432539048517
1108	7	0.6373301636834938
1108	8	0.7209356008489323
1108	9	0.9796476878513829
1108	10	1.2212105321106463
1108	11	0.7649613264846877
1108	12	0.5732283306659909
1108	13	0.6090793453497696
1108	14	1.6578528910245183
1108	15	1.237784537815763
1108	16	1.2316579613628988
1108	17	1.6252846801345342
1108	18	1.8401848310342217
1108	19	1.1490498216288785
1108	20	1.04348368585282
1108	21	0.9174503286795854
1108	22	1.173943753575621
1108	23	1.466174889854969
1108	24	1.201680871468902
1108	25	1.5685315823051909
1109	1	0.4774868200438114
1109	2	0.6571075178822525
1109	3	0.5672592619768402
1109	4	0.8464055395698153
1109	5	0.9634495553342788
1109	6	0.5534581543683075
1109	7	0.6169561090279458
1109	8	0.6214732744464655
1109	9	0.8472942239970322
1109	10	1.1188433923806897
1109	11	0.6844720937517934
1109	12	0.7185286354725946
1109	13	0.5995320155069521
1109	14	1.5628658566606148
1109	15	1.002525742562696
1109	16	1.2304772408896767
1109	17	1.5266015087627167
1109	18	1.6912103168856376
1109	19	1.1531570421321646
1109	20	0.9508749598706885
1109	21	0.8509197420554884
1109	22	1.1190978418142308
1109	23	1.4354737479175483
1109	24	1.3025392191084322
1109	25	1.406237818328563
1110	1	0.23230282477920383
1110	2	0.46437048789903557
1110	3	0.31370127787102575
1110	4	0.6828701116995362
1110	5	0.55830900170848
1110	6	0.31511211872009426
1110	7	0.19232883942537882
1110	8	0.12838770305024383
1110	9	0.2544016929678463
1110	10	0.7647572826186178
1110	11	0.32755185592296243
1110	12	0.1364533529937404
1110	13	0.014734437077272844
1110	14	0.8264102673700435
1110	15	0.578321379707738
1110	16	0.7938923978529999
1110	17	1.1285911290497594
1110	18	0.6783730780725676
1110	19	0.45836066907134665
1110	20	0.29939557814633133
1110	21	0.2809206355034206
1110	22	0.4699096281213144
1110	23	-0.9090247829711515
1110	24	-0.012655670417757392
1110	25	0.420794732835752
1111	1	0.3645382740106875
1111	2	0.7103880830798488
1111	3	0.6496329804104732
1111	4	1.0287308679588492
1111	5	0.7771598260012382
1111	6	0.5367017110752172
1111	7	0.4186825552937421
1111	8	0.572548281591402
1111	9	0.739818096750767
1111	10	1.0638103418514362
1111	11	0.5675251929567722
1111	12	0.4712689720111314
1111	13	0.31595523134797787
1111	14	1.2372322345129803
1111	15	0.8653798325869104
1111	16	0.9850527227441077
1111	17	1.3451123809508232
1111	18	1.6478528135075265
1111	19	1.0203153054435532
1111	20	0.8009343146568284
1111	21	0.6274156916577169
1111	22	0.9340160488231675
1111	23	1.1562271292787472
1111	24	0.9323597099565291
1111	25	1.045915805002977
1112	1	0.004386494463428647
1112	2	0.19111193743840715
1112	3	0.1660828239014549
1112	4	0.47768231795662075
1112	5	0.2763611190441466
1112	6	-0.06984133855513619
1112	7	-0.0031925235356666803
1112	8	0.0884009281067577
1112	9	0.3381343767275027
1112	10	0.5356135517206511
1112	11	0.015343963268357186
1112	12	-0.06933778246265376
1112	13	-0.1144585909578808
1112	14	0.527169254284189
1112	15	0.3374363525288899
1112	16	0.2535809935678941
1112	17	0.6068066768749532
1112	18	0.6300870834389158
1112	19	0.07857462761207756
1112	20	0.06352628328438925
1112	21	-0.0442490482958533
1112	22	0.19273832184168072
1112	23	0.6334462938811356
1112	24	0.26043156976518844
1112	25	0.5741983615363537
1113	1	0.20007480525728738
1113	2	0.4095010004226687
1113	3	0.3544378931925074
1113	4	0.6938830676872811
1113	5	0.6716131938401091
1113	6	0.2951431896409389
1113	7	0.2262823698343297
1113	8	0.3384100291678962
1113	9	0.4254623789707921
1113	10	0.7191471493947148
1113	11	0.37926661900718983
1113	12	0.357778337238436
1113	13	0.22456147073255295
1113	14	0.77860636103226
1113	15	0.5881256964161707
1113	16	0.5563713297168178
1113	17	1.0185143311133373
1113	18	0.9169216605839381
1113	19	0.7095916588951354
1113	20	0.5014090812949448
1113	21	0.28283411080545307
1113	22	0.6057849389526595
1113	23	0.620395495201528
1113	24	0.6089904044358008
1113	25	1.1152906364572743
1114	1	-0.7853548799471248
1114	2	-0.4348015324379162
1114	3	-0.47933456072431824
1114	4	-0.04275868827267715
1114	5	-3.308807088633589
1114	6	-1.145281696253008
1114	7	-0.6697616271097822
1114	8	-0.43756109661321574
1114	9	-0.22351752269231895
1114	10	0.0063902883396522725
1114	11	-0.33030555674551465
1114	12	-0.5386207107350742
1114	13	-0.4797397888415844
1114	14	-6.020618403409749
1114	15	-2.289891013019858
1114	16	-1.0418930829063484
1114	17	-0.13707441695224531
1114	18	-6.198221143388718
1114	19	-2.433612419539312
1114	20	-1.3810469903225169
1114	21	-1.018744274667327
1114	22	-0.7052431908833796
1114	23	-0.2055535929294834
1114	24	-0.36378673125735617
1114	25	-0.14714055710185647
1115	1	-0.8329163819592544
1115	2	-0.5252947202885458
1115	3	-0.6230986547665758
1115	4	-3.291916461422108
1115	5	-1.2054936195343053
1115	6	-0.892769836300257
1115	7	-0.8187714026842308
1115	8	-0.6045817208661077
1115	9	-0.41837887367327653
1115	10	-0.720680276700314
1115	11	-0.8026155832975448
1115	12	-0.7902598921378186
1115	13	-0.9159089743153572
1115	14	-6.176799148950714
1115	15	-2.5892034298566173
1115	16	-1.5169736351774432
1115	17	-0.5769655411270946
1115	18	-0.41238389301739176
1115	19	-0.7461133168802618
1115	20	-0.8052455316976932
1115	21	-0.9013088589008973
1115	22	-1.9928780178088914
1115	23	-1.2252312958477987
1115	24	-0.9813844788968282
1115	25	-0.49201496873565986
1116	1	-0.17740134602112434
1116	2	0.1759537145561616
1116	3	0.14157195608546402
1116	4	0.38253756548800055
1116	5	-0.3203281097721664
1116	6	-0.03650932855175881
1116	7	-0.032789486906686705
1116	8	0.09709833685554514
1116	9	0.05967790879368806
1116	10	0.28168773893410304
1116	11	-0.03114324065273877
1116	12	-0.3256284318089797
1116	13	-0.2791824881804814
1116	14	0.6331449406635059
1116	15	0.2138276492590805
1116	16	0.25639354980680906
1116	17	0.7318239125171431
1116	18	0.4183726577104778
1116	19	0.2844851034635809
1116	20	-0.21192777021929743
1116	21	-0.3585615380969216
1116	22	-0.28399672341307536
1116	23	-0.9944514386785528
1116	24	-0.576051434983647
1116	25	0.007748683412359014
1201	1	0.8818074169160184
1201	2	1.0757920143886537
1201	3	1.0450725849334148
1201	4	1.4548015237250524
1201	5	1.329667339189065
1201	6	0.9522654463226843
1201	7	0.8744798294870577
1201	8	1.0736025306000485
1201	9	1.3218548290428913
1201	10	1.7974677744070604
1201	11	1.2211635828378604
1201	12	1.2437012378962393
1201	13	1.0635551099874334
1201	14	2.120405049375499
1201	15	1.8122752924495984
1201	16	2.0089650124302096
1201	17	2.2144618253353343
1201	18	1.442266365559938
1201	19	1.5182808910623322
1201	20	1.5685030899471428
1201	21	1.4246831715090877
1201	22	1.543195305781964
1201	23	1.8704621645020438
1201	24	1.5937137515309985
1201	25	1.9672363923389895
1202	1	0.7235923589855524
1202	2	0.8590831004965445
1202	3	0.8379295182945086
1202	4	1.265410225146951
1202	5	1.0392375377782699
1202	6	0.775964697363996
1202	7	0.7183905554581855
1202	8	0.7769374570192014
1202	9	0.9624115163731943
1202	10	1.298581572181007
1202	11	0.9130324984801206
1202	12	0.7950772938164405
1202	13	0.7679382035325091
1202	14	1.8126413043224403
1202	15	1.2624528558072114
1202	16	1.3891442442006294
1202	17	1.6338969594165818
1202	18	1.9160874299688473
1202	19	1.4501181741746763
1202	20	1.219930183913398
1202	21	1.0980729728429495
1202	22	1.2028614501280401
1202	23	1.4679169454663707
1202	24	1.1261366138830908
1202	25	1.559488321711754
1203	1	0.6602496474136714
1203	2	0.7427112966829874
1203	3	0.7659726187874583
1203	4	1.0506751997902413
1203	5	1.009148405107716
1203	6	0.6701650449104832
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2211	13	-0.6780533591401579
2211	14	-0.029334929321549907
2211	15	-0.36372924768140535
2211	16	-7.146097089043167
2211	17	-2.2154215867996356
2211	18	-0.8874936629725241
2211	19	-0.8854049530791173
2211	20	-1.0170145187244088
2211	21	-0.8950941114490334
2211	22	-0.7186536167803652
2211	23	-0.3129003278467266
2211	24	-0.8229635184140136
2211	25	-7.883608255174305
2212	1	-1.374716997329351
2212	2	-3.7457797302830933
2212	3	-1.68625654592174
2212	4	-3.780192036891144
2212	5	-1.3082357910775677
2212	6	-0.9661301726033713
2212	7	-0.8921531753166825
2212	8	-0.8802030114737747
2212	9	-0.8071363468994193
2212	10	-0.247145977212341
2212	11	-0.8922861307337584
2212	12	-0.9101995573772115
2212	13	-1.1039538994175047
2212	14	-6.263124267973382
2212	15	-4.986057824326814
2212	16	-2.842342067834707
2212	17	-7.518019038038904
2212	18	-3.0880971443437204
2212	19	-1.9334418829554245
2212	20	-1.9158876144106216
2212	21	-1.570562289394104
2212	22	-1.456219512826138
2212	23	-0.8092635215693988
2212	24	-1.2658570453857436
2212	25	-1.086005560342052
2213	1	-0.2889778643365233
2213	2	-0.1951862322853195
2213	3	-0.21018262342949257
2213	4	0.23046904780550648
2213	5	-0.01789612930523532
2213	6	-0.28284905532684235
2213	7	-0.4576544494620123
2213	8	-0.3643745596063148
2213	9	-0.023834821234487436
2213	10	-4.151131404519866
2213	11	-1.5646386479521865
2213	12	-0.9519140274761639
2213	13	-0.6772485119706815
2213	14	0.05156021098758856
2213	15	-0.47738104171843787
2213	16	-0.3114072717163481
2213	17	0.30226487757700937
2213	18	0.3443593637258644
2213	19	-0.16953200345684394
2213	20	-0.6575073050120963
2213	21	-0.5438461461750279
2213	22	-0.7747452733603595
2213	23	-1.0604530915959103
2213	24	-0.7575907287872852
2213	25	-0.39716683441383793
2214	1	-0.867562914412062
2214	2	-0.693794574579826
2214	3	-0.5966846367899521
2214	4	-0.24058226293974627
2214	5	-3.5301597285406103
2214	6	-1.4592763413825303
2214	7	-1.07121802232626
2214	8	-0.6856762366551905
2214	9	-0.7749289497643588
2214	10	-4.141029417940448
2214	11	-1.8245221276475583
2214	12	-1.261211148104266
2214	13	-0.9283912608442151
2214	14	-0.3663308457385739
2214	15	-0.5284571112635206
2214	16	-0.41749936661778975
2214	17	-0.17330710034411823
2214	18	0.27741837356516186
2214	19	-0.5290474487850361
2214	20	-0.9043927563933494
2214	21	-0.8591930267074375
2214	22	-0.5570340777462519
2214	23	-0.2122238285710054
2214	24	-0.8065790840441807
2214	25	-0.3810204395838568
2215	1	-2.0471059466986326
2215	2	-4.508771312562207
2215	3	-2.6791588891007194
2215	4	-1.7242706228665732
2215	5	-1.8369662233494637
2215	6	-1.9836488562029366
2215	7	-2.193213244303678
2215	8	-2.3225137943463317
2215	9	-7.981782037209733
2215	10	-3.9220380658621714
2215	11	-3.165343158954144
2215	12	-3.3915832605388303
2215	13	-2.770207291792616
2215	14	-7.865196056941091
2215	15	-4.700133264211974
2215	16	-9.343735676867844
2215	17	-4.435678637427664
2215	18	-2.9139519683941977
2215	19	-3.355457432722215
2215	20	-3.069270410517639
2215	21	-3.222688578814438
2215	22	-9.37617682496641
2215	23	-10.682986358734482
2215	24	-6.879669244225912
2215	25	-5.230604159174231
2216	1	-1.8259994989094395
2216	2	-1.9304504910032279
2216	3	-1.7214004872073794
2216	4	-6.719220275432871
2216	5	-3.5551382682726107
2216	6	-2.711076403851539
2216	7	-2.1596239036575753
2216	8	-7.792285591349433
2216	9	-4.030421413208266
2216	10	-2.220276350314517
2216	11	-2.2203884784118273
2216	12	-2.415253897796479
2216	13	-2.20828594947179
2216	14	-1.3723511632338443
2216	15	-2.1316019511071005
2216	16	-1.735622391590553
2216	17	-1.330230403153287
2216	18	-7.5488288512146156
2216	19	-4.797372229166367
2216	20	-4.242326409866184
2216	21	-3.242539632155836
2216	22	-2.7882310367268666
2216	23	-2.0012216700254832
2216	24	-2.5020393862586836
2216	25	-1.9536040677869906
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	105348.0
3	2.0
4	857.0
5	4913.0
6	6075.0
7	5371.0
8	4982.0
9	4670.0
10	4374.0
11	4170.0
12	3887.0
13	3919.0
14	4045.0
15	4039.0
16	4085.0
17	4378.0
18	4807.0
19	6044.0
20	7719.0
21	9803.0
22	12012.0
23	14135.0
24	16278.0
25	18533.0
26	20994.0
27	23360.0
28	26035.0
29	31007.0
30	36919.0
31	44910.0
32	56332.0
33	72582.0
34	94472.0
35	124910.0
36	176156.0
37	250612.0
38	643385.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.50705395937572	17.827107431415417	12.158952492024797	25.506886117184074
2	14.927599013911966	19.590127452526687	40.51538439932676	24.96688913423458
3	18.73304203765093	24.353997177071513	29.245668781792382	27.667292003485176
4	11.857951147706887	16.2641822909761	38.1091180664731	33.76874849484391
5	13.317575275693988	37.463647716666095	35.62469751443288	13.59407949320704
6	32.862571438732694	36.222172168558224	17.684582166262704	13.230674226446368
7	28.68907581137952	31.163817026710994	21.735648968902733	18.41145819300675
8	25.85757424227402	35.515536022967794	20.331864796941076	18.29502493781711
9	26.72915255534199	14.850313534100772	19.510997807389778	38.90953610316746
10	14.505241296882165	28.06029714243837	34.845773364091905	22.588688196587565
11	34.93858521281514	22.12694437049978	23.86247657271323	19.071993843971846
12	23.558718822853557	24.307153566004853	30.849275115514825	21.284852495626765
13	29.10800019616117	21.207609113303015	25.3442386009613	24.340152089574516
14	21.244012228553014	20.886025811882263	27.99024819780811	29.879713761756616
15	23.95060118101833	29.12262281792957	23.020533075196674	23.90624292585542
16	23.227786369784397	27.152710793057178	26.54944415405258	23.07005868310584
17	22.39639870772594	27.44600163636305	27.352487476648406	22.8051121792626
18	22.51645350228398	26.64195497571586	28.472427314646147	22.369164207354007
19	23.932298711698827	26.63548933996256	26.909790894912867	22.522421053425752
20	24.295027562973946	26.44283236031643	26.917996582180454	22.34414349452917
21	23.961653421345645	26.089707453201243	26.43818532814371	23.510453797309403
22	23.975991022505184	26.699746564717625	26.319863138479583	23.00439927429761
23	23.21095983134078	26.465582654658178	26.921990799622325	23.401466714378717
24	23.701208222271934	26.471629819885134	26.751383270312445	23.075778687530487
25	23.53007436535064	26.241784940881757	26.786724830955993	23.441415862811613
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	186.0
1	186.0
2	314.0
3	442.0
4	442.0
5	442.0
6	878.5
7	1315.0
8	1315.0
9	1315.0
10	1941.0
11	2567.0
12	2567.0
13	2567.0
14	4667.5
15	6768.0
16	6768.0
17	6768.0
18	12470.5
19	18173.0
20	18173.0
21	18173.0
22	28276.5
23	38380.0
24	38380.0
25	38380.0
26	54766.5
27	71153.0
28	71153.0
29	71153.0
30	92099.0
31	113045.0
32	113045.0
33	113045.0
34	133276.0
35	153507.0
36	153507.0
37	153507.0
38	172850.0
39	192193.0
40	192193.0
41	192193.0
42	208101.5
43	224010.0
44	224010.0
45	224010.0
46	237027.0
47	250044.0
48	250044.0
49	250044.0
50	245801.5
51	241559.0
52	241559.0
53	241559.0
54	221128.0
55	200697.0
56	200697.0
57	200697.0
58	174605.5
59	148514.0
60	148514.0
61	148514.0
62	122803.5
63	97093.0
64	97093.0
65	97093.0
66	75666.5
67	54240.0
68	54240.0
69	54240.0
70	39845.0
71	25450.0
72	25450.0
73	25450.0
74	17927.5
75	10405.0
76	10405.0
77	10405.0
78	7333.0
79	4261.0
80	4261.0
81	4261.0
82	2867.0
83	1473.0
84	1473.0
85	1473.0
86	974.0
87	475.0
88	475.0
89	475.0
90	304.0
91	133.0
92	133.0
93	133.0
94	77.5
95	22.0
96	22.0
97	22.0
98	18.5
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17181001228368856
2	0.2566644397991509
3	0.26135163674762407
4	0.4474387431847079
5	0.2581729629549813
6	0.017994526215977417
7	0.017779022908001638
8	0.016378251406159082
9	0.018371657004935024
10	0.020311186776717022
11	0.020365062603710966
12	0.0276921750748874
13	0.02709954097795401
14	0.024944507898196234
15	0.04159213843932504
16	0.021011572527638297
17	0.042346400017240264
18	0.031678986272439286
19	0.02683016184298429
20	0.02968558067366334
21	0.03351076439023339
22	0.04385492317307071
23	0.02930844988470573
24	0.049296381699459095
25	0.03695881731784583
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1856120.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.5228595662083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.84389212363307	61.64442706090192
2	10.979514751017895	16.144906422884024
3	2.632996516077498	5.8075629926964485
4	1.0121952651403954	2.97677961329993
5	0.4877431979484247	1.793013732356768
6	0.2683005804974146	1.183573554086615
7	0.16207375324628837	0.8341288059506609
8	0.12084272819069321	0.7107762347489454
9	0.08670808411665377	0.5737523662587342
>10	0.37592173401473733	4.75175648138663
>50	0.0187643265083479	0.9569925624588739
>100	0.009268242993113988	1.3181233774370704
>500	0.0011116853846664335	0.5529597059148809
>1k	6.670112307998559E-4	0.7512470896184892
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	2502	0.1347973191388488	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2103	0.11330086416826497	No Hit
CCCATGTACTCTGCGTTGATACCAC	2094	0.1128159817253195	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.6162748098183307E-4	0.0
10	1.0775165398788871E-4	0.0	0.0	1.6162748098183307E-4	0.0
11	1.0775165398788871E-4	0.0	0.0	2.693791349697218E-4	0.0
12	1.0775165398788871E-4	0.0	0.0	3.2325496196366615E-4	0.0
13	1.0775165398788871E-4	0.0	0.0	3.2325496196366615E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAA	205	0.0	13.509835	1
ACTGCGT	50	0.0015143547	13.282891	8
TGGCGAG	765	0.0	11.164501	18
GTCCTAC	905	0.0	10.974649	1
TAGGACT	260	0.0	10.628346	4
GTCCTAA	495	0.0	10.418243	1
TTAGACT	175	2.237357E-10	10.345622	4
CTAACAC	150	2.3332177E-8	10.163845	3
TATGGCG	850	0.0	10.159969	16
TAGGACC	405	0.0	10.1170635	4
GAGTACG	85	6.6644506E-4	10.047238	11
CTTATAC	200	2.3646862E-11	10.005034	3
TTAGGAC	460	0.0	9.942891	3
GGCGAGA	525	0.0	9.941722	19
GTATTAG	125	2.4029996E-6	9.932058	1
ATGGCGA	855	0.0	9.878565	17
GCGCCAC	125	2.6072303E-6	9.868887	13
TTTAGAC	155	3.8782673E-8	9.835979	3
GTCCTAT	185	5.8935257E-10	9.808166	1
TCCTACA	1070	0.0	9.802393	2
>>END_MODULE