FastQCFastQC Report
Thu 26 May 2016
SRR1513130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856120
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32430.17471930694136154No Hit
GTACATGGGAAGCAGTGGTATCAAC28390.15295347283580804No Hit
CCCATGTACTCTGCGTTGATACCAC27390.1475658901364136No Hit
TATCAACGCAGAGTACTTTTTTTTT22920.12348339547012047No Hit
GGTATCAACGCAGAGTACTTTTTTT19570.10543499342714911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC653.404406E-614.60232517
GAACCGT602.5222276E-514.2776666
TTGCGCG602.5911919E-514.23688318
TAGGACC3850.012.8571224
TGGCGAG8300.011.09219118
TATCCTA1303.1708623E-810.9837115
TCAGGAC4100.010.9128633
TATACTG2550.010.8257765
TGCGCGC803.8112266E-410.67536119
GTAGGAC8350.010.4888053
GACGTGA4350.010.4736747
GTATTAC1303.7006248E-710.2586731
CTCTATA2002.3646862E-119.9970672
TAGGACA4000.09.9951764
ATGGCGA9500.09.99106417
GGCGAGG3800.09.98864219
CTTATCC2105.456968E-129.9730513
GACAGCG1151.0371123E-59.9044537
TGTAGGA10000.09.9018582
GGACGTG7200.09.78284556
Produced by FastQC (version 0.11.5)