##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513130_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1856120
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.678066612072495	31.0	31.0	34.0	27.0	34.0
2	30.79267773635325	33.0	31.0	34.0	27.0	34.0
3	30.884715966640087	33.0	31.0	34.0	27.0	34.0
4	34.26793634032283	37.0	35.0	37.0	30.0	37.0
5	34.01546613365515	37.0	35.0	37.0	30.0	37.0
6	33.927740663319184	37.0	35.0	37.0	30.0	37.0
7	33.79200428851583	37.0	35.0	37.0	29.0	37.0
8	33.75562302006336	37.0	35.0	37.0	29.0	37.0
9	35.303253561192165	39.0	35.0	39.0	29.0	39.0
10	35.144624808740815	39.0	35.0	39.0	28.0	39.0
11	35.15702325280693	39.0	35.0	39.0	28.0	39.0
12	35.089383768290844	39.0	35.0	39.0	28.0	39.0
13	35.05688910199772	39.0	35.0	39.0	27.0	39.0
14	36.21549145529384	40.0	36.0	41.0	27.0	41.0
15	36.18116016205849	40.0	36.0	41.0	27.0	41.0
16	36.14423474775338	40.0	36.0	41.0	27.0	41.0
17	36.07500323254962	40.0	36.0	41.0	27.0	41.0
18	36.027351141090016	40.0	36.0	41.0	27.0	41.0
19	36.10459776307567	40.0	37.0	41.0	27.0	41.0
20	36.070545007865874	40.0	37.0	41.0	27.0	41.0
21	36.016135271426414	40.0	37.0	41.0	27.0	41.0
22	35.959107708553326	40.0	36.0	41.0	27.0	41.0
23	35.87532271620369	40.0	36.0	41.0	27.0	41.0
24	35.811189470508374	40.0	36.0	41.0	27.0	41.0
25	35.75057323879921	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3571761068448076
1101	2	0.3085387851844281
1101	3	0.25680845390857954
1101	4	0.40930931337199894
1101	5	0.4037635998160525
1101	6	0.4640622146447271
1101	7	0.4862087526520824
1101	8	0.3822536395332534
1101	9	0.5379430030027024
1101	10	0.6079577898779647
1101	11	0.6452275535062597
1101	12	0.5760925990249319
1101	13	0.5399010238753306
1101	14	0.6158588499978634
1101	15	0.7448371686360318
1101	16	0.6098767683506594
1101	17	0.6618492029992993
1101	18	0.7177081049851992
1101	19	0.6192367571655879
1101	20	0.6724559758215918
1101	21	0.6248453676765919
1101	22	0.762149259697182
1101	23	0.7387670944065903
1101	24	0.8071904945878501
1101	25	0.7771382452384117
1102	1	0.5844894739637922
1102	2	0.591420984852391
1102	3	0.5073039504715986
1102	4	0.6217329619978926
1102	5	0.7501178801704427
1102	6	0.7810998125123163
1102	7	0.6583564199827379
1102	8	0.7237422621338823
1102	9	0.7038229038659694
1102	10	0.711792540370972
1102	11	0.7565876151736006
1102	12	0.7323849999973433
1102	13	0.725605277599513
1102	14	0.848529317654318
1102	15	0.6793187401553027
1102	16	0.8374474569069008
1102	17	0.8193366581114887
1102	18	0.7774364892984451
1102	19	0.8783968639857918
1102	20	0.8119633605601706
1102	21	0.7737618860165156
1102	22	0.7801913217112713
1102	23	0.8215105924564057
1102	24	0.9530176589006203
1102	25	0.9140665532158749
1103	1	0.4545211869563026
1103	2	0.44280830554634676
1103	3	0.42950586797854484
1103	4	0.45826807210971765
1103	5	0.4925203848095663
1103	6	0.26022544701762484
1103	7	0.38703335982623344
1103	8	0.4714272382264113
1103	9	0.4449586070009417
1103	10	0.5387959571268155
1103	11	0.5003989179590391
1103	12	0.44122536484402985
1103	13	0.502399933518447
1103	14	0.5532629481537725
1103	15	0.6358029671142873
1103	16	0.5026733751600361
1103	17	0.6419519245300833
1103	18	0.5814657820888911
1103	19	0.5771469989869189
1103	20	0.5058194591748162
1103	21	0.4626493294387686
1103	22	0.38312955992199704
1103	23	0.44886746671389943
1103	24	0.5456086742072941
1103	25	0.5447361097248873
1104	1	0.390275376900437
1104	2	0.49064350107707355
1104	3	0.4672153651852504
1104	4	0.6960474017186584
1104	5	0.516263401569347
1104	6	0.5695829889170625
1104	7	0.6115445330105942
1104	8	0.5646534952096545
1104	9	0.5434222941517817
1104	10	0.5929160688586634
1104	11	0.6428570185177023
1104	12	0.623487934676433
1104	13	0.5889921122893966
1104	14	0.7582210487124286
1104	15	0.7080795034271432
1104	16	0.7236929282326656
1104	17	0.7941865614015882
1104	18	0.6422199720330255
1104	19	0.6574542615567509
1104	20	0.742900464761405
1104	21	0.732551564075635
1104	22	0.7825709007041155
1104	23	0.7439093661552434
1104	24	0.6877176127547813
1104	25	0.7088422549762896
1105	1	0.41700557271730077
1105	2	0.5616151507497911
1105	3	0.4591787188376486
1105	4	0.539305109183303
1105	5	0.6847258701682009
1105	6	0.5868775469724312
1105	7	0.6292792744703704
1105	8	0.5026528795202267
1105	9	0.6417548759624978
1105	10	0.5857346289347518
1105	11	0.6679475959041383
1105	12	0.5708526556724252
1105	13	0.7451193320767118
1105	14	0.7479325478964469
1105	15	0.7871439169730934
1105	16	0.6961007425549397
1105	17	0.8149383290419792
1105	18	0.6976923311299359
1105	19	0.7448177788009929
1105	20	0.6852858962077448
1105	21	0.7169169747621922
1105	22	0.702993165967591
1105	23	0.7536063512504256
1105	24	0.7385773579146075
1105	25	0.715692786501755
1106	1	0.1977715324685363
1106	2	0.208830340467415
1106	3	0.15459273267400775
1106	4	0.21978749865673564
1106	5	0.3060690874354606
1106	6	0.14899629941090353
1106	7	0.07649815650931657
1106	8	0.21340381152366916
1106	9	0.18204253240923407
1106	10	0.22793860014941458
1106	11	0.1808657974482628
1106	12	0.25750823716995797
1106	13	0.22174496884829153
1106	14	0.3665914475600758
1106	15	0.24974365580290225
1106	16	0.2107131042005861
1106	17	0.2097869254060285
1106	18	0.11747861957161376
1106	19	0.16909023765314402
1106	20	0.31192845611873565
1106	21	0.26966030796834417
1106	22	0.1709006167027951
1106	23	0.15550568134025866
1106	24	0.27444653650849915
1106	25	0.295671912578527
1107	1	0.2829816725374883
1107	2	0.3841965727236989
1107	3	0.3726071462566303
1107	4	0.41976075255782064
1107	5	0.3830606031820736
1107	6	0.34513631218552376
1107	7	0.35172528462557295
1107	8	0.24457828279970073
1107	9	0.38164866862381785
1107	10	0.4531414857970191
1107	11	0.3316093756366385
1107	12	0.40547058745592324
1107	13	0.33113443723598834
1107	14	0.37685327843435346
1107	15	0.3719224003483461
1107	16	0.4024972888292737
1107	17	0.41338836700854387
1107	18	0.4958293012307493
1107	19	0.47113881785053024
1107	20	0.5448508277038755
1107	21	0.4925397026027625
1107	22	0.5569271844943842
1107	23	0.6113532456744011
1107	24	0.6364246975249017
1107	25	0.5311805952904152
1108	1	0.15406757807182458
1108	2	0.339686630611471
1108	3	0.322810381666951
1108	4	0.46813452397677935
1108	5	0.3666797648967375
1108	6	0.3293807670252704
1108	7	0.3227491697083025
1108	8	0.436383465644937
1108	9	0.4705085039782375
1108	10	0.4893284020381543
1108	11	0.4072002994481636
1108	12	0.4931451403761855
1108	13	0.43713246552114526
1108	14	0.4900258819933043
1108	15	0.5873690184769487
1108	16	0.5956648861661833
1108	17	0.6257580728711929
1108	18	0.6109823429649808
1108	19	0.5692218782466227
1108	20	0.5307907586126817
1108	21	0.5114178363529689
1108	22	0.37236709050534955
1108	23	0.43524254468094625
1108	24	0.3935851276771416
1108	25	0.44699367808001966
1109	1	0.13464448532394258
1109	2	0.21267098714953647
1109	3	0.10860407341480993
1109	4	0.22937320577706544
1109	5	0.23717147593781363
1109	6	0.24069841854333163
1109	7	0.12595631976594746
1109	8	0.2493036078566888
1109	9	0.1933700354666641
1109	10	0.23120893071659054
1109	11	0.25976337251174897
1109	12	0.1966371934647526
1109	13	0.2919085461002595
1109	14	0.3075480629719678
1109	15	0.2077240129305764
1109	16	0.22756997744491514
1109	17	0.24125669172944697
1109	18	0.26775509415332266
1109	19	0.22460752384229465
1109	20	0.34273499920881534
1109	21	0.26453195648067407
1109	22	0.32187213368779055
1109	23	0.36639148088192286
1109	24	0.3124742094705866
1109	25	0.2204029373622589
1110	1	-0.012270272126706061
1110	2	0.08006010137509989
1110	3	0.03188687918467181
1110	4	-0.04955628159539316
1110	5	-0.07975324484761614
1110	6	-0.04844176416253987
1110	7	-0.0392289038384348
1110	8	-0.15043757031217808
1110	9	-0.10983463773752788
1110	10	-0.06584340441657588
1110	11	-0.191192078771941
1110	12	-0.1946917915941171
1110	13	-0.23607360235065045
1110	14	-0.1855621719395515
1110	15	-0.17994534169655907
1110	16	-0.13812865833626375
1110	17	-0.09084709282652881
1110	18	-0.0944054152788425
1110	19	-0.21718385981355937
1110	20	-0.1643086710511099
1110	21	-0.103120779289533
1110	22	-0.02881657684196881
1110	23	-0.15958542705558187
1110	24	-0.17935787503048317
1110	25	-0.09478699528095547
1111	1	0.14501046884369373
1111	2	0.23267540582914847
1111	3	0.23310686951171533
1111	4	0.2380444254555769
1111	5	0.15000441507512363
1111	6	0.11372870195351226
1111	7	0.04296932513194207
1111	8	0.10536646597434185
1111	9	0.1849478494800607
1111	10	0.15876295738766544
1111	11	0.1975892486695372
1111	12	0.23244126026572332
1111	13	0.1367515407192812
1111	14	0.16380855572616326
1111	15	0.3150160870674483
1111	16	0.3256699335039883
1111	17	0.33082042380665655
1111	18	0.21176044808498773
1111	19	0.198360285956106
1111	20	0.24740544052531988
1111	21	0.22744221080296967
1111	22	0.327202531850169
1111	23	0.22290393438128575
1111	24	0.2791999362666928
1111	25	0.3723584489625864
1112	1	-0.66139035639101
1112	2	-0.6430272539031527
1112	3	-0.41005632212949905
1112	4	-1.3619415421754084
1112	5	-0.6623803473208483
1112	6	-0.6824205156304544
1112	7	-0.5241765578871451
1112	8	-0.4429092832174817
1112	9	-0.46116730206424705
1112	10	-0.3205496817994202
1112	11	-0.3530147474347274
1112	12	-0.4440192412553685
1112	13	-0.33113832771083196
1112	14	-0.43004435149089204
1112	15	-0.46519888272482746
1112	16	-0.3626237503695009
1112	17	-0.36198837425406083
1112	18	-0.3956186773112407
1112	19	-0.5019004509474172
1112	20	-0.44438037906182615
1112	21	-0.47361341991388883
1112	22	-0.5077717634544712
1112	23	-0.49897580234988226
1112	24	-0.6488123681029023
1112	25	-0.5019623274732652
1113	1	-0.4984766885649812
1113	2	-0.3589286525148694
1113	3	-0.42718328931156435
1113	4	-1.4273808774535794
1113	5	-0.9034440891852356
1113	6	-0.6845243366002833
1113	7	-0.47214124085044773
1113	8	-0.38557665665123153
1113	9	-0.39015528866355
1113	10	-0.4081609238236865
1113	11	-0.4854319892720582
1113	12	-0.4305800486424758
1113	13	-0.2997412656317593
1113	14	-0.3270226762069157
1113	15	-0.3944924927960116
1113	16	-0.3579942619475247
1113	17	-0.49316983957409377
1113	18	-0.4531917440736706
1113	19	-0.357186937593454
1113	20	-0.4061985988942709
1113	21	-0.4942119499695252
1113	22	-0.414973162558141
1113	23	-0.4286701050856081
1113	24	-0.468144374813086
1113	25	-0.38862410744404485
1114	1	-0.6709110163676826
1114	2	-0.6062297749658505
1114	3	-0.4456684702161837
1114	4	-1.3146382184396899
1114	5	-0.772511064052523
1114	6	-0.5921581785952341
1114	7	-0.5696676370580178
1114	8	-0.5501494200746109
1114	9	-0.5895122790061578
1114	10	-0.43454243962223416
1114	11	-0.42384312367234145
1114	12	-0.42022676062508424
1114	13	-0.37901852865356034
1114	14	-0.47298684788511025
1114	15	-0.4559843927352816
1114	16	-0.4962320335003483
1114	17	-0.5133426147508899
1114	18	-0.552152546440027
1114	19	-0.4956088459560064
1114	20	-0.552919886284819
1114	21	-0.5209240305195877
1114	22	-0.4795944088117352
1114	23	-0.5177169032599167
1114	24	-0.4990612390023941
1114	25	-0.48503735745873655
1115	1	-0.3177698367313688
1115	2	-0.24688142743947594
1115	3	-0.21396716271515714
1115	4	0.12964918168915318
1115	5	-0.14989364901507685
1115	6	-0.19646474361111643
1115	7	-0.3587657665594861
1115	8	-0.30192638673335637
1115	9	-0.3824978053018384
1115	10	-0.34444998687331463
1115	11	-0.31513819855572933
1115	12	-0.44346071257947983
1115	13	-0.5351006245826682
1115	14	-0.503672434898931
1115	15	-0.48161522921451905
1115	16	-0.5092985914208654
1115	17	-0.5701461496489983
1115	18	-0.6467725763869012
1115	19	-0.665781394779458
1115	20	-0.6211612184343522
1115	21	-0.5106057674196833
1115	22	-0.5671142139949623
1115	23	-0.5172118450358028
1115	24	-0.5149258796931804
1115	25	-0.45061188492478266
1116	1	-0.2532974380890174
1116	2	-0.26928940072589924
1116	3	-0.19939024924975257
1116	4	-0.1170311130370365
1116	5	-0.4042382049014037
1116	6	-0.4689442660009959
1116	7	-0.4826509646648347
1116	8	-0.4708135531106663
1116	9	-0.5435268085682026
1116	10	-0.5017176027282417
1116	11	-0.519410799386236
1116	12	-0.4681141994057896
1116	13	-0.4859482710201277
1116	14	-0.4975294205092311
1116	15	-0.5386761317397486
1116	16	-0.41483299964441045
1116	17	-0.49109958368956086
1116	18	-0.7044648542297693
1116	19	-0.6478310172019803
1116	20	-0.7188704889654218
1116	21	-0.6133703388110021
1116	22	-0.7118100822476663
1116	23	-0.6757814933867365
1116	24	-0.676355581818477
1116	25	-0.6174445741669388
1201	1	0.5368293976013838
1201	2	0.6237457247444773
1201	3	0.5648499762001613
1201	4	0.6812268700129849
1201	5	0.6086600927083694
1201	6	0.5752518520886483
1201	7	0.6484190775818846
1201	8	0.6015304883223962
1201	9	0.6227770740964615
1201	10	0.6132423908751647
1201	11	0.588098816883246
1201	12	0.7047373959973129
1201	13	0.6815582424969548
1201	14	0.7427771183528691
1201	15	0.767633418351096
1201	16	0.7403493763196636
1201	17	0.8367720840574577
1201	18	0.9332492017414751
1201	19	0.830038236734417
1201	20	0.8753616602764609
1201	21	0.8107408382887726
1201	22	0.8383168439606408
1201	23	0.7928003727604249
1201	24	0.6737540705575924
1201	25	0.801267838904451
1202	1	0.47005141526540584
1202	2	0.42313122474664056
1202	3	0.35625498172707637
1202	4	0.48767728088969164
1202	5	0.47931578855911994
1202	6	0.4207176397694212
1202	7	0.42922797892483544
1202	8	0.4534325270074504
1202	9	0.4588904944340797
1202	10	0.45351927047201457
1202	11	0.44970854195093324
1202	12	0.4755521680377939
1202	13	0.4822635676186877
1202	14	0.5515716011473017
1202	15	0.6028649118305864
1202	16	0.6015148323853552
1202	17	0.6813406791432826
1202	18	0.6386542582343111
1202	19	0.6109423385778001
1202	20	0.5553132041833635
1202	21	0.5074122104885959
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2210	16	-0.021221451169814998
2210	17	0.003580090406870795
2210	18	-0.07662011341340502
2210	19	-0.1052852680548213
2210	20	-0.06193244585876556
2210	21	-0.10135946939684004
2210	22	-0.04592323779893093
2210	23	-0.07099981641729869
2210	24	-0.1494952200259192
2210	25	-0.15602039619780328
2211	1	-0.7787027182828083
2211	2	-0.6972702139107376
2211	3	-0.5238751241695105
2211	4	-1.466142312040482
2211	5	-0.7490202955644065
2211	6	-0.7560673403271139
2211	7	-0.6313433607865591
2211	8	-0.6769263009085336
2211	9	-0.6000688940069452
2211	10	-0.48900405823150805
2211	11	-0.5642495682856534
2211	12	-0.6027800873627669
2211	13	-0.5603760364560131
2211	14	-0.683986279130302
2211	15	-0.5319834177270408
2211	16	-0.5313813196891601
2211	17	-0.5109932750322059
2211	18	-0.6216032440010721
2211	19	-0.7172203338472656
2211	20	-0.6473014012755698
2211	21	-0.7260339818169683
2211	22	-0.6863128847400262
2211	23	-0.725337739759631
2211	24	-0.624541693432711
2211	25	-0.7112464393737667
2212	1	-0.14236508707594098
2212	2	-0.46427654367655435
2212	3	-0.42812641399974893
2212	4	-0.07452176090140483
2212	5	-0.268509138883978
2212	6	-0.2808957531102436
2212	7	-0.2978550605534096
2212	8	-0.2310001512975859
2212	9	-0.38093703637049714
2212	10	-0.264994020496367
2212	11	-0.4460318280869586
2212	12	-0.47540197749076896
2212	13	-0.32619576922004256
2212	14	-0.4715893953227024
2212	15	-0.3770998535819814
2212	16	-0.41153361797469756
2212	17	-0.4407328744591581
2212	18	-0.34929643763010176
2212	19	-0.45639348477847363
2212	20	-0.49813358757293713
2212	21	-0.3890338788178198
2212	22	-0.4309592699381142
2212	23	-0.4691774543583591
2212	24	-0.47068016174174687
2212	25	-0.577461080234464
2213	1	-0.42014991279565805
2213	2	-0.46889187313413316
2213	3	-0.5628507352829395
2213	4	-0.327827552103372
2213	5	-0.41602225724894737
2213	6	-0.45720400435037334
2213	7	-0.5176901754838923
2213	8	-0.5827070096192486
2213	9	-0.5864692724312022
2213	10	-0.5632092977378917
2213	11	-0.5524578964017408
2213	12	-0.5987440257575258
2213	13	-0.6821465611022859
2213	14	-0.6205292622820764
2213	15	-0.6770075561857922
2213	16	-0.789982819473515
2213	17	-0.669077919633402
2213	18	-0.7352411253817621
2213	19	-0.7385463491233608
2213	20	-0.6806806463748316
2213	21	-0.5940223508362692
2213	22	-0.7899308710221504
2213	23	-0.8235590066461214
2213	24	-0.7652063010262395
2213	25	-0.7562886642857691
2214	1	-0.6147936588986269
2214	2	-0.6591243763007171
2214	3	-0.6768091918751047
2214	4	-0.5242656963229209
2214	5	-0.6173011430847382
2214	6	-0.6271374571052917
2214	7	-0.6313245723616205
2214	8	-0.721687635721743
2214	9	-0.9500433208730072
2214	10	-1.0155915351664007
2214	11	-0.9062804346524018
2214	12	-0.9424818849047369
2214	13	-0.8733732193520538
2214	14	-1.0661528164815763
2214	15	-1.0440278419140014
2214	16	-1.0588448939202593
2214	17	-1.155271677737737
2214	18	-1.0395642823746911
2214	19	-1.1127038052523304
2214	20	-1.0956683740079995
2214	21	-1.1598599894042465
2214	22	-1.202966155955579
2214	23	-1.2413611348280185
2214	24	-1.2802040260040641
2214	25	-1.332393507509643
2215	1	-0.5451120824008413
2215	2	-0.5167494024057504
2215	3	-0.43410046715369077
2215	4	-0.3169277890464315
2215	5	-0.6541410399669019
2215	6	-0.7264561339823814
2215	7	-0.9329342394283913
2215	8	-0.8964074360627947
2215	9	-0.9393561419002623
2215	10	-1.0713342322513952
2215	11	-1.114630941733914
2215	12	-1.0271594752185962
2215	13	-1.144535841148837
2215	14	-1.2072817213139473
2215	15	-1.2042314999814039
2215	16	-1.1649851241483873
2215	17	-1.2465827864321852
2215	18	-1.2819748727021576
2215	19	-1.193309403813636
2215	20	-1.1950026829789167
2215	21	-1.2230306301277807
2215	22	-1.2192973080307752
2215	23	-1.2903816941803257
2215	24	-1.2534716835505506
2215	25	-1.293459584820134
2216	1	-0.5514217169089299
2216	2	-0.5058560882268246
2216	3	-0.6035074646421634
2216	4	-0.31618159042560734
2216	5	-0.664145761454499
2216	6	-0.8007111182502413
2216	7	-1.0586499709754094
2216	8	-0.90160800756302
2216	9	-1.0872921717276256
2216	10	-1.0465498339882657
2216	11	-0.9728530919201717
2216	12	-1.0964180271682125
2216	13	-1.0738656527736765
2216	14	-1.308431623343921
2216	15	-1.2771362573639493
2216	16	-1.2285276671454142
2216	17	-1.383451340597695
2216	18	-1.3050153071371824
2216	19	-1.3201414690791111
2216	20	-1.3342121822406057
2216	21	-1.5625843360145666
2216	22	-1.4205608680458468
2216	23	-1.5881727740027713
2216	24	-1.561421847639302
2216	25	-1.6770742443176516
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	47214.0
3	1.0
4	774.0
5	6357.0
6	7096.0
7	5466.0
8	4537.0
9	3882.0
10	3412.0
11	3187.0
12	2991.0
13	2823.0
14	2747.0
15	2706.0
16	2726.0
17	2928.0
18	3429.0
19	3929.0
20	5109.0
21	6511.0
22	8172.0
23	9831.0
24	11431.0
25	12714.0
26	14123.0
27	15687.0
28	17688.0
29	21362.0
30	26713.0
31	34530.0
32	44269.0
33	57254.0
34	76010.0
35	101741.0
36	145999.0
37	237762.0
38	903009.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.272361254077346	18.791890157307545	13.371222160267687	27.56452642834742
2	14.26284581253317	20.079312267808216	38.808915086842084	26.848926832816527
3	17.25984294392586	24.293853800460663	29.548686036017834	28.897617219595638
4	11.671571284334568	16.750786875954297	36.64433637882972	34.93330546088141
5	13.209643894114507	37.57840449250396	35.53326917011072	13.678682443270814
6	32.938065448664275	36.56704517751378	17.283262584400063	13.211626789421876
7	28.320709214125	31.212696872936963	21.86179486143109	18.604799051506944
8	25.937812186466452	35.83563864123518	19.88456038218359	18.341988790114783
9	26.44621464573674	14.64201914295797	19.19161007200834	39.72015613929694
10	14.828779135078578	28.48900327738486	34.12661168619646	22.5556059013401
11	35.02235846820248	22.0498135896386	23.71403788548154	19.21379005667737
12	23.342294679223325	24.40526474581385	30.868909337758332	21.383531237204494
13	29.058681550761804	20.97854664569101	25.477878585436287	24.484893218110898
14	21.600199127845894	20.89129106607747	26.992706188371923	30.515803617704705
15	23.90292653492231	29.201290864814776	22.942805422063227	23.952977178199685
16	23.351238066504322	27.27571493222421	26.136133439648297	23.236913561623172
17	22.574015307175447	27.69625554565829	26.78976640472137	22.93996274244489
18	22.75280777770592	26.864666192416305	27.852508137705062	22.53001789217271
19	23.905189319656056	26.522315367540894	26.94109217076482	22.63140314203823
20	24.14181195181346	26.332079822425275	26.976326961618863	22.549781264142403
21	23.807679190908715	26.067139448318443	26.57842462745952	23.546756733313327
22	23.887194793440077	26.554263732948304	26.30745856948904	23.251082904122576
23	23.14336791266483	26.49882382859686	26.656095671279758	23.701712587458555
24	23.51663685537573	26.468224037238976	26.88058961705062	23.134549490334678
25	23.365391298070108	26.33842586431782	26.7493374609467	23.54684537666537
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	126.0
1	126.0
2	266.5
3	407.0
4	407.0
5	407.0
6	882.0
7	1357.0
8	1357.0
9	1357.0
10	1934.5
11	2512.0
12	2512.0
13	2512.0
14	4508.5
15	6505.0
16	6505.0
17	6505.0
18	12079.5
19	17654.0
20	17654.0
21	17654.0
22	27716.5
23	37779.0
24	37779.0
25	37779.0
26	54493.0
27	71207.0
28	71207.0
29	71207.0
30	91230.5
31	111254.0
32	111254.0
33	111254.0
34	131225.5
35	151197.0
36	151197.0
37	151197.0
38	170489.0
39	189781.0
40	189781.0
41	189781.0
42	204898.5
43	220016.0
44	220016.0
45	220016.0
46	235396.0
47	250776.0
48	250776.0
49	250776.0
50	247864.0
51	244952.0
52	244952.0
53	244952.0
54	223636.0
55	202320.0
56	202320.0
57	202320.0
58	176373.0
59	150426.0
60	150426.0
61	150426.0
62	124985.0
63	99544.0
64	99544.0
65	99544.0
66	77383.0
67	55222.0
68	55222.0
69	55222.0
70	40639.0
71	26056.0
72	26056.0
73	26056.0
74	18388.0
75	10720.0
76	10720.0
77	10720.0
78	7443.5
79	4167.0
80	4167.0
81	4167.0
82	2843.5
83	1520.0
84	1520.0
85	1520.0
86	1005.0
87	490.0
88	490.0
89	490.0
90	306.0
91	122.0
92	122.0
93	122.0
94	66.0
95	10.0
96	10.0
97	10.0
98	5.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18894252526776284
2	0.008889511454000819
3	0.005118203564424714
4	0.0024782880417214405
5	0.07946684481606793
6	0.33139021183975176
7	0.029362325711699677
8	0.013899963364437645
9	0.00457944529448527
10	0.0036096804085942722
11	0.0
12	0.0
13	0.0
14	0.0014007715018425533
15	0.0
16	0.0
17	0.020957696700644356
18	0.03017046311660884
19	0.0
20	0.0
21	7.003857509212766E-4
22	0.0
23	0.005279831045406547
24	0.0
25	1.6162748098183307E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1856120.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.96289200018322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.80932291107953	57.23616823435972
2	12.005937297031075	16.799401889463134
3	3.1395509889813584	6.589562003135136
4	1.1961704843507743	3.347501856417603
5	0.5738160652397652	2.0072915700169887
6	0.33090882226479595	1.3890802916411844
7	0.2024680738182411	0.9915676387421508
8	0.1368963936332693	0.7662134082383191
9	0.09817992347468957	0.6182056244381364
>10	0.4675804558741171	5.665218931613122
>50	0.024527368429475345	1.1963672463056918
>100	0.012099257620727938	1.5115755818169552
>500	0.001617609765080056	0.7377240503239447
>1k	9.243484371885967E-4	1.1441216734880018
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3243	0.17471930694136154	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2839	0.15295347283580804	No Hit
CCCATGTACTCTGCGTTGATACCAC	2739	0.1475658901364136	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2292	0.12348339547012047	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1957	0.10543499342714911	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.3875826993944355E-5	0.0
8	0.0	0.0	0.0	5.3875826993944355E-5	0.0
9	0.0	0.0	0.0	5.3875826993944355E-5	0.0
10	0.0	0.0	0.0	5.3875826993944355E-5	0.0
11	0.0	0.0	0.0	5.3875826993944355E-5	0.0
12	0.0	0.0	0.0	5.3875826993944355E-5	0.0
13	0.0	0.0	0.0	5.3875826993944355E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTGCGC	65	3.404406E-6	14.602325	17
GAACCGT	60	2.5222276E-5	14.277666	6
TTGCGCG	60	2.5911919E-5	14.236883	18
TAGGACC	385	0.0	12.857122	4
TGGCGAG	830	0.0	11.092191	18
TATCCTA	130	3.1708623E-8	10.983711	5
TCAGGAC	410	0.0	10.912863	3
TATACTG	255	0.0	10.825776	5
TGCGCGC	80	3.8112266E-4	10.675361	19
GTAGGAC	835	0.0	10.488805	3
GACGTGA	435	0.0	10.473674	7
GTATTAC	130	3.7006248E-7	10.258673	1
CTCTATA	200	2.3646862E-11	9.997067	2
TAGGACA	400	0.0	9.995176	4
ATGGCGA	950	0.0	9.991064	17
GGCGAGG	380	0.0	9.988642	19
CTTATCC	210	5.456968E-12	9.973051	3
GACAGCG	115	1.0371123E-5	9.904453	7
TGTAGGA	1000	0.0	9.901858	2
GGACGTG	720	0.0	9.7828455	6
>>END_MODULE