FastQCFastQC Report
Thu 26 May 2016
SRR1513129_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513129_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2167418
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC24760.11423730909312371No Hit
GTATCAACGCAGAGTACTTTTTTTT22650.1045022233828454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTT400.00531892114.23422813
GTGCGAA604.131594E-412.65264711
GCGTAGG550.002945389112.1575091
TAGGACC3600.011.64611154
GTCCTAC9350.010.7272141
ATTACAC2350.010.542813
TTAGAGT2200.010.3948774
TTACACC2151.8189894E-1210.1934264
ACGAAAT951.6629789E-49.98893212
GTAAGAC1451.5471596E-79.8576673
TTACACT3100.09.8360134
TTATACT3450.09.6667344
TGGCGAG8650.09.65404318
AGGACCT8150.09.5684655
TCCTACA12200.09.5359322
TAGGACT3200.09.5286374
TATGGCG8700.09.48970516
GGCGAGG3800.09.48970419
TCGAACT1901.0732037E-99.48970419
ACCGTGC1401.0783133E-69.4872958
Produced by FastQC (version 0.11.5)