##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513129_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2167418
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.182814759312695	31.0	30.0	34.0	23.0	34.0
2	29.044514256133336	31.0	30.0	34.0	16.0	34.0
3	29.23734784891516	31.0	30.0	34.0	22.0	34.0
4	32.301128808563924	35.0	33.0	37.0	19.0	37.0
5	32.429687766734425	35.0	33.0	37.0	22.0	37.0
6	32.78029434100852	37.0	33.0	37.0	25.0	37.0
7	32.72222201716512	37.0	35.0	37.0	25.0	37.0
8	32.604053302131845	37.0	33.0	37.0	24.0	37.0
9	33.810728710382584	39.0	34.0	39.0	22.0	39.0
10	33.385388974346434	38.0	33.0	39.0	17.0	39.0
11	33.82683220310987	38.0	34.0	39.0	23.0	39.0
12	33.806618289596194	38.0	34.0	39.0	23.0	39.0
13	33.84866878470143	39.0	34.0	39.0	23.0	39.0
14	34.04425957521807	39.0	33.0	41.0	12.0	41.0
15	34.4547258535271	39.0	34.0	41.0	17.0	41.0
16	34.29963901748532	39.0	34.0	41.0	17.0	41.0
17	33.89555591030433	39.0	33.0	41.0	11.0	41.0
18	33.62811695759655	39.0	32.0	41.0	10.0	41.0
19	34.25726740296519	39.0	34.0	41.0	18.0	41.0
20	34.46318568914718	39.0	34.0	41.0	18.0	41.0
21	34.503845589544795	39.0	34.0	41.0	18.0	41.0
22	34.187595101637065	39.0	34.0	41.0	10.0	41.0
23	33.71605523253936	39.0	33.0	41.0	8.0	41.0
24	33.9512927363342	39.0	34.0	41.0	2.0	41.0
25	33.58543714225867	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9503094769459892
1101	2	1.2583930973482111
1101	3	1.1470636428946257
1101	4	1.5462514362373412
1101	5	1.3447739865055937
1101	6	1.0118415883924285
1101	7	0.9517079524534324
1101	8	1.0366931932181274
1101	9	1.4688433713505447
1101	10	1.8305625687921179
1101	11	1.4353968764887526
1101	12	1.2853088687672312
1101	13	1.003046998549081
1101	14	2.0516514754379642
1101	15	1.8630039214899625
1101	16	1.952250034138892
1101	17	2.15167592058728
1101	18	2.1278526945163136
1101	19	1.7337922477862335
1101	20	1.4344185796220685
1101	21	1.3396767294404555
1101	22	1.6161470816903076
1101	23	1.901756917381988
1101	24	1.7070928555888898
1101	25	1.898814401263671
1102	1	0.9218463591093986
1102	2	1.0436153676256694
1102	3	0.9638823345824008
1102	4	1.2624376454949555
1102	5	1.2968241787067143
1102	6	0.9475954845754728
1102	7	0.8976583953826918
1102	8	0.9238914476049302
1102	9	1.295904056724197
1102	10	1.554442456662123
1102	11	1.1192745370831148
1102	12	1.0943739745130543
1102	13	1.0249864147737568
1102	14	2.0684741672076186
1102	15	1.6030098671515702
1102	16	1.818850871602045
1102	17	2.022500318682944
1102	18	2.235372266278439
1102	19	1.5477677826845024
1102	20	1.4588046186978048
1102	21	1.3439313705213678
1102	22	1.6749189889462457
1102	23	2.0145460805780075
1102	24	1.6163832992805993
1102	25	1.9186455114216088
1103	1	0.8611485600897524
1103	2	1.223427972835939
1103	3	0.8790620911990601
1103	4	1.3917950192982502
1103	5	1.210414286901802
1103	6	1.0209291763343984
1103	7	0.9959732697977728
1103	8	0.9810965436753492
1103	9	1.46476941723855
1103	10	1.672219192121787
1103	11	1.346225979066375
1103	12	1.1901010470163769
1103	13	1.2675954626717711
1103	14	2.0416912470524835
1103	15	1.6939636376652558
1103	16	1.7157633440712488
1103	17	2.117062940578542
1103	18	2.3679144987013743
1103	19	1.774023909908287
1103	20	1.3503059461084632
1103	21	1.4876664927692147
1103	22	1.6428187323629615
1103	23	2.082127968467418
1103	24	1.7093917754279957
1103	25	1.9227767990099451
1104	1	0.852235814552234
1104	2	1.0440318132521682
1104	3	0.9156854864327109
1104	4	1.1836064854318948
1104	5	1.121494707098897
1104	6	0.9476612584337687
1104	7	0.7794774291463895
1104	8	0.8601847938829508
1104	9	1.103096602182589
1104	10	1.291504129547846
1104	11	0.9629014029010534
1104	12	0.8426065768924005
1104	13	0.9005938496381702
1104	14	1.8702698668248203
1104	15	1.4125625765169545
1104	16	1.6911287568094409
1104	17	2.0390058928342185
1104	18	2.2354340094267613
1104	19	1.5701439027065902
1104	20	1.1562794063778625
1104	21	1.165613402285011
1104	22	1.5343031825720246
1104	23	1.6806416994619156
1104	24	1.406111328040815
1104	25	1.6489351335068179
1105	1	0.8471593842962051
1105	2	1.0391219423142388
1105	3	0.8987622222646081
1105	4	1.2578017759532116
1105	5	1.1298264880497584
1105	6	0.9405012548532099
1105	7	0.9350309913663466
1105	8	0.9088364915504314
1105	9	1.1297451625796953
1105	10	1.5031999993047833
1105	11	1.001487055611662
1105	12	0.9823440756850843
1105	13	0.8878524340030651
1105	14	1.6893908207128305
1105	15	1.3506506169810066
1105	16	1.6199461428479722
1105	17	1.937346087356893
1105	18	1.5880132874749293
1105	19	1.3530016362976554
1105	20	1.286178925514598
1105	21	1.1571566553206267
1105	22	1.4178765443215582
1105	23	1.671058955677232
1105	24	1.596839612846459
1105	25	1.8558866680950459
1106	1	0.5784755671807069
1106	2	0.748734040028026
1106	3	0.631524349620026
1106	4	1.1160611349342062
1106	5	0.9982501741490637
1106	6	0.6973865171904379
1106	7	0.5178936630566113
1106	8	0.6626706766984114
1106	9	0.8070668786920123
1106	10	0.7879811951513602
1106	11	0.6455060900042966
1106	12	0.6794214424705558
1106	13	0.5506610558164553
1106	14	1.5513328209659747
1106	15	1.1761855793716265
1106	16	1.1800067196283166
1106	17	1.480970245943226
1106	18	1.5600549017660725
1106	19	1.1640866052022076
1106	20	0.874430462218605
1106	21	0.8913576850591767
1106	22	1.0245442524759483
1106	23	1.498913915354791
1106	24	1.1024991349444377
1106	25	1.3413135307319024
1107	1	0.3260845112948054
1107	2	0.5869507107908625
1107	3	0.47597751714252823
1107	4	0.793005763876792
1107	5	0.6394355497278355
1107	6	0.44389291998013647
1107	7	0.4013407685142667
1107	8	0.39876880474424325
1107	9	0.7375885404045803
1107	10	0.9898001615128607
1107	11	0.5858008310111558
1107	12	0.512836832630974
1107	13	0.3204489151241745
1107	14	1.185654065127153
1107	15	0.6963793420031479
1107	16	0.8813628321147533
1107	17	1.3601195305722555
1107	18	1.5139888661675514
1107	19	0.9075501006771631
1107	20	0.7440618665093481
1107	21	0.6432066321833574
1107	22	0.9367124037707697
1107	23	1.2469089045957844
1107	24	0.9631667314995269
1107	25	1.2858342205714166
1108	1	0.7845342058314557
1108	2	0.9259524176034866
1108	3	0.7718931405974843
1108	4	1.2571293401153767
1108	5	1.1474290264605287
1108	6	0.7228802141011101
1108	7	0.6657258233218215
1108	8	0.7894731930156169
1108	9	1.024237448207984
1108	10	1.3246591252394495
1108	11	0.9575441790590986
1108	12	0.7591861402518063
1108	13	0.8783725154870083
1108	14	1.7976997527411172
1108	15	1.311681096136418
1108	16	1.4056164937204798
1108	17	1.7784229420325062
1108	18	2.022630339637729
1108	19	1.392115309712004
1108	20	1.1823281372320125
1108	21	1.242696753392238
1108	22	1.4819090053446757
1108	23	1.6571979404786177
1108	24	1.3892107569263032
1108	25	1.696180164936301
1109	1	0.5965445906397413
1109	2	0.755095054053843
1109	3	0.6759555173918699
1109	4	0.9971524234271811
1109	5	1.1954811149996019
1109	6	0.7770278346515198
1109	7	0.8018683712652859
1109	8	0.7972676996880494
1109	9	0.970951473510226
1109	10	1.3719174232072788
1109	11	0.8562260522224747
1109	12	0.7614633599196452
1109	13	0.8498849194145706
1109	14	1.7905017288585583
1109	15	1.2407897602456188
1109	16	1.4714109950288616
1109	17	1.7127141761850808
1109	18	1.8749365698968887
1109	19	1.2527466409718286
1109	20	1.1655767334144116
1109	21	1.1042025148330978
1109	22	1.3106251101118005
1109	23	1.6308199373907115
1109	24	1.4573134106131533
1109	25	1.6634706114669697
1110	1	0.5148710767227556
1110	2	0.7370018783879964
1110	3	0.721404575398612
1110	4	0.9703504819454878
1110	5	0.7698282224258435
1110	6	0.5367660027792454
1110	7	0.5507759361458966
1110	8	0.619296704394678
1110	9	0.7922507933434986
1110	10	1.1546662162803685
1110	11	0.7780239722466575
1110	12	0.6527070167982458
1110	13	0.5794663406191987
1110	14	1.2803351958250104
1110	15	0.9883114936549475
1110	16	1.1656127601967583
1110	17	1.5111968661119235
1110	18	1.170134253423413
1110	19	0.9466132104896303
1110	20	0.8342227014603409
1110	21	0.7570412258086208
1110	22	0.9683165460009349
1110	23	-0.32272799950454356
1110	24	0.5522553305105617
1110	25	0.911684993523771
1111	1	0.3144666020318567
1111	2	0.7358524656556362
1111	3	0.6715797570299245
1111	4	1.0374426714523395
1111	5	0.8962303343146161
1111	6	0.5612001473852359
1111	7	0.6120007373154408
1111	8	0.6287714923874574
1111	9	0.7964368437258997
1111	10	1.0910617952674428
1111	11	0.8137806861554964
1111	12	0.7267419747574309
1111	13	0.5222512639026817
1111	14	1.3998759404648382
1111	15	1.1258119964525832
1111	16	1.0784613723365766
1111	17	1.5273247937811902
1111	18	1.808071496649859
1111	19	1.2516809078567732
1111	20	0.8586687224932348
1111	21	0.6401439830815008
1111	22	0.9816750122808955
1111	23	1.3553670840361463
1111	24	1.0042287938916417
1111	25	1.369504346308787
1112	1	0.1748703989151288
1112	2	0.33175838296716265
1112	3	0.23436555537253412
1112	4	0.6092570057393658
1112	5	0.39244706375291116
1112	6	0.21625569204452688
1112	7	0.16453414160402957
1112	8	0.3008388962206823
1112	9	0.5519895952078713
1112	10	0.6665871361681184
1112	11	0.23575739573363563
1112	12	0.3342655004014432
1112	13	0.30390870227173394
1112	14	0.8938031114349982
1112	15	0.5973435155350657
1112	16	0.46474320619156373
1112	17	1.0142650782148337
1112	18	1.142780359518774
1112	19	0.5952135159560967
1112	20	0.3430079334086358
1112	21	0.24862948503048443
1112	22	0.6167734489793943
1112	23	0.94896561262901
1112	24	0.7016652153329233
1112	25	0.952498247677326
1113	1	0.21331951916986824
1113	2	0.27035302643095704
1113	3	0.30720994126294343
1113	4	0.5166779944893491
1113	5	0.589586667964177
1113	6	0.3292631111830744
1113	7	0.3410653557807848
1113	8	0.40944126970917694
1113	9	0.5268055586860854
1113	10	0.7917680195277867
1113	11	0.37082609167003966
1113	12	0.35011591647551654
1113	13	0.17781956924104492
1113	14	0.9082136703412331
1113	15	0.6482055839764058
1113	16	0.7819378508888235
1113	17	1.0453905234252545
1113	18	0.9061668139339076
1113	19	0.6474419546465953
1113	20	0.6013782975762751
1113	21	0.5130606958456099
1113	22	0.6269427876831202
1113	23	0.6248787462267558
1113	24	0.4353599143636018
1113	25	0.8909727582705145
1114	1	-0.9820051408032597
1114	2	-0.6974802196034382
1114	3	-0.5964673641678502
1114	4	-0.3681300627673316
1114	5	-3.3701171980440137
1114	6	-1.313338057437793
1114	7	-0.9998647698054874
1114	8	-0.5891180622333678
1114	9	-0.37593422894407524
1114	10	-0.16081970519916666
1114	11	-0.5649358224937586
1114	12	-0.6394249139451134
1114	13	-0.6424769844709317
1114	14	-6.075157787573897
1114	15	-2.473417499604871
1114	16	-1.415621005228104
1114	17	-0.30473738415408036
1114	18	-6.442697992915583
1114	19	-2.419041548423575
1114	20	-1.3229172899227422
1114	21	-0.956628184439495
1114	22	-0.8930196602105482
1114	23	-0.4520236675496605
1114	24	-0.3880950764303961
1114	25	-0.28654514515015705
1115	1	-0.9017007560619916
1115	2	-0.44567508230512587
1115	3	-0.5927294261945448
1115	4	-3.126259657618693
1115	5	-1.1515827115922832
1115	6	-0.8517624490171514
1115	7	-0.8271475227648146
1115	8	-0.5088135142918802
1115	9	-0.3438422917288335
1115	10	-0.696517184204481
1115	11	-0.741120421533239
1115	12	-0.7223436847046969
1115	13	-0.901539309413188
1115	14	-5.779291445294881
1115	15	-2.535251535583175
1115	16	-1.4723877557932852
1115	17	-0.5312281264172682
1115	18	-0.3036290420441574
1115	19	-0.6383358135268367
1115	20	-0.6756465076999802
1115	21	-0.8070697838506646
1115	22	-1.9833988028952803
1115	23	-1.162833764910836
1115	24	-0.9701058419332895
1115	25	-0.543562326461938
1116	1	-0.14266114826074983
1116	2	0.09017083857513697
1116	3	0.021674869096752758
1116	4	0.3011265441299926
1116	5	-0.31472625623733563
1116	6	-0.11259890613065693
1116	7	0.0055804848361376
1116	8	0.12810483073327816
1116	9	0.058053924299230175
1116	10	0.3460343455991506
1116	11	0.021741058633899968
1116	12	-0.2540754470414015
1116	13	-0.16602034683708666
1116	14	0.7760844996736651
1116	15	0.2600081209178171
1116	16	0.3700239514399968
1116	17	0.7684919184644627
1116	18	0.5509185899047964
1116	19	0.2549027664643546
1116	20	-0.08294669142595978
1116	21	-0.11229843276499452
1116	22	-0.12234300262124265
1116	23	-0.7926462141094817
1116	24	-0.5490120338715627
1116	25	0.02685819139265533
1201	1	0.5887995968422786
1201	2	0.8689347594603092
1201	3	0.8104230192615844
1201	4	1.0271431356106646
1201	5	0.9615770581624759
1201	6	0.6391447807414323
1201	7	0.49852605465397914
1201	8	0.6768882422258997
1201	9	0.799585320301631
1201	10	1.301788926823093
1201	11	0.7487599121882198
1201	12	0.6250846655092772
1201	13	0.5663119660186524
1201	14	1.4068134954421936
1201	15	1.253700976357564
1201	16	1.2183580113520662
1201	17	1.4859312050303046
1201	18	0.7794353229029127
1201	19	0.8443705032234448
1201	20	0.7732780381123519
1201	21	0.6789808579765335
1201	22	1.0764239454711344
1201	23	1.3104479167598058
1201	24	1.0685241870384345
1201	25	1.415762096514463
1202	1	0.6195750659190082
1202	2	0.7722077255520929
1202	3	0.7565988823897563
1202	4	1.0864121483357252
1202	5	0.99025760274003
1202	6	0.6576450204367816
1202	7	0.5710657072049727
1202	8	0.6984762464054697
1202	9	0.8643698965164006
1202	10	1.1416126704146166
1202	11	0.7651247426471386
1202	12	0.7125991725401235
1202	13	0.6786943835615276
1202	14	1.6261257432961784
1202	15	1.1792431183985812
1202	16	1.238135769422989
1202	17	1.5603813516107579
1202	18	1.7420222847611484
1202	19	1.2072184952447387
1202	20	0.9991366037188314
1202	21	0.8692449855432969
1202	22	1.1243321697848572
1202	23	1.4040916844879447
1202	24	0.994523870871916
1202	25	1.4531050896346258
1203	1	0.66427374553869
1203	2	0.72612365855192
1203	3	0.7507432970622148
1203	4	1.0588451943620427
1203	5	1.012070716787271
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2211	15	-0.31335791242248945
2211	16	-7.000899741061804
2211	17	-2.0844726419578983
2211	18	-0.8150656058794041
2211	19	-0.8421793958472392
2211	20	-0.9496282432114072
2211	21	-0.8325848413520518
2211	22	-0.725782410146266
2211	23	-0.33756867563558046
2211	24	-0.6885560058119324
2211	25	-7.987272984307271
2212	1	-1.7087095645652006
2212	2	-4.137667252451774
2212	3	-1.9111085555741525
2212	4	-3.5304582471258854
2212	5	-1.4715179286043316
2212	6	-1.2622103420892223
2212	7	-1.208107637015356
2212	8	-1.0882429520991792
2212	9	-1.2320480310578148
2212	10	-0.6094934333329647
2212	11	-1.169914685967676
2212	12	-1.212732920963468
2212	13	-1.3561303488028216
2212	14	-6.704043645293538
2212	15	-5.410840447665205
2212	16	-2.967634161697191
2212	17	-7.367354836129692
2212	18	-3.0617821803751077
2212	19	-1.959546826045731
2212	20	-2.0457585967337195
2212	21	-1.6566438069066152
2212	22	-1.5893052926642923
2212	23	-0.9594324126035971
2212	24	-1.3367231970391984
2212	25	-1.2265614227530008
2213	1	-0.24595263399416822
2213	2	-0.2827197480243022
2213	3	-0.2355335643975991
2213	4	0.10315161776140513
2213	5	-0.06418434693800634
2213	6	-0.26546478079283276
2213	7	-0.33253713837828514
2213	8	-0.4426113167965724
2213	9	-0.1678750885124174
2213	10	-3.9504682281971455
2213	11	-1.5593555272628308
2213	12	-1.0199037599725713
2213	13	-0.7798511727842978
2213	14	0.08643003248661074
2213	15	-0.36892240186351444
2213	16	-0.08076691557582194
2213	17	0.23005800818988575
2213	18	0.22557929482234584
2213	19	-0.2664903856588339
2213	20	-0.7183943729641413
2213	21	-0.69877562133059
2213	22	-0.9842185775345627
2213	23	-1.2608273544020037
2213	24	-0.747334177435647
2213	25	-0.29420580526339535
2214	1	-1.1165982880309002
2214	2	-0.7711245954518908
2214	3	-0.7191907730431382
2214	4	-0.29631335059619346
2214	5	-3.740414229375684
2214	6	-1.6974721502619872
2214	7	-1.3390168361774535
2214	8	-0.9957992623671075
2214	9	-0.929287063607589
2214	10	-4.363235205261059
2214	11	-2.0505706787979037
2214	12	-1.5598050377222208
2214	13	-1.326037099932627
2214	14	-0.6420801264668796
2214	15	-0.9245239800753708
2214	16	-0.8757520531799088
2214	17	-0.6579973592571946
2214	18	-0.24537858275885327
2214	19	-1.0509699388153635
2214	20	-1.2964162899757952
2214	21	-1.243002298253728
2214	22	-1.106570905075614
2214	23	-0.9176561303965869
2214	24	-1.332042528940626
2214	25	-1.0578517054848149
2215	1	-2.4630077076357146
2215	2	-5.000536859835641
2215	3	-3.210004840167926
2215	4	-2.18718257571026
2215	5	-2.190457806121451
2215	6	-2.3110759286003706
2215	7	-2.4628398328681342
2215	8	-2.601631760351875
2215	9	-8.390463140900984
2215	10	-4.290075192449965
2215	11	-3.5109634707351027
2215	12	-3.9181802916109625
2215	13	-3.199995291724143
2215	14	-8.3649735288976
2215	15	-5.270401081563328
2215	16	-9.789483394442648
2215	17	-4.968714143936488
2215	18	-3.355320044690572
2215	19	-3.857080741254503
2215	20	-3.5284164021615325
2215	21	-3.732987222488866
2215	22	-9.592540031108399
2215	23	-10.684434708787258
2215	24	-6.893954792921409
2215	25	-5.172061544722329
2216	1	-1.8786360282583132
2216	2	-1.856145197895767
2216	3	-1.6964797662937485
2216	4	-6.6893930731503595
2216	5	-3.349731894800186
2216	6	-2.646637923463235
2216	7	-2.154479387567225
2216	8	-8.107230221688372
2216	9	-4.0789865738148094
2216	10	-2.3364292262401243
2216	11	-2.210428084199151
2216	12	-2.4140048215532275
2216	13	-2.2237135159201955
2216	14	-1.4601832869767009
2216	15	-2.0857679631441997
2216	16	-1.833815950848333
2216	17	-1.5515239696355394
2216	18	-7.112454816454658
2216	19	-4.73216418254032
2216	20	-4.1234865238031375
2216	21	-3.1807593246767283
2216	22	-2.813153682164682
2216	23	-1.954028704234677
2216	24	-2.5149372265956487
2216	25	-1.9079399972409838
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	109221.0
3	2.0
4	894.0
5	5413.0
6	6560.0
7	5952.0
8	5250.0
9	5131.0
10	4790.0
11	4661.0
12	4483.0
13	4437.0
14	4523.0
15	4597.0
16	4654.0
17	4952.0
18	5498.0
19	6734.0
20	8794.0
21	11043.0
22	13857.0
23	16496.0
24	19124.0
25	21434.0
26	24364.0
27	27243.0
28	30550.0
29	36244.0
30	43738.0
31	51670.0
32	65555.0
33	84926.0
34	111350.0
35	146529.0
36	205402.0
37	292806.0
38	768541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.391386823796516	17.89310350565479	12.0973378026842	25.618171867864493
2	14.731229499741657	19.89529401261782	40.66501901369328	24.70845747394724
3	18.500490548215964	24.539298671009828	29.46060049022442	27.499610290549786
4	11.622961616773988	16.474989235380626	38.624520342929024	33.277528804916365
5	13.062315706703362	37.658483562483376	35.96165543079823	13.317545300015032
6	32.46598984771574	36.61568989386249	17.953068758652517	12.965251499769268
7	28.295822458163666	31.28167950767323	22.248197521384245	18.174300512778863
8	25.56938134648796	35.752003304038446	20.685172666003705	17.993442683469887
9	26.63603456932323	14.843553877050752	19.69471270987463	38.82569884375138
10	14.042154752631324	28.426400389853473	35.36858873781246	22.162856119702738
11	34.85254233924838	22.190754702092008	24.052994031733842	18.903708926925773
12	23.224449616417154	24.532780261463067	31.26231362553287	20.980456496586914
13	28.99021388239583	21.360364954576816	25.485763864051194	24.16365729897616
14	21.02563416239246	20.895677367488837	28.296430710784293	29.782257759334414
15	23.770633300746542	29.267415701294517	23.192563934268843	23.769387063690097
16	23.010414075684444	27.367371321535916	26.7009572347761	22.921257368003538
17	22.257809164445366	27.4502255916733	27.68271771615836	22.609247527722967
18	22.185807935637634	26.993186051620597	28.643782433637387	22.177223579104382
19	23.69692611999605	26.760099278303223	27.089657833211344	22.453316768489383
20	24.014299698305162	26.72073820854096	27.038816961184885	22.22614513196899
21	23.878288634109442	26.328826718642045	26.572657450360392	23.220227196888125
22	23.678208670003947	26.840823749616305	26.604952375677094	22.876015204702657
23	22.92174335231091	26.721411798586654	27.143653589124444	23.213191259977993
24	23.329015795173284	26.678831476607286	27.03315887771089	22.95899385050855
25	23.328833226023082	26.394398635659773	27.042737172963356	23.23403096535379
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	208.0
1	208.0
2	356.0
3	504.0
4	504.0
5	504.0
6	980.0
7	1456.0
8	1456.0
9	1456.0
10	2318.0
11	3180.0
12	3180.0
13	3180.0
14	5842.0
15	8504.0
16	8504.0
17	8504.0
18	15506.5
19	22509.0
20	22509.0
21	22509.0
22	35417.0
23	48325.0
24	48325.0
25	48325.0
26	68619.0
27	88913.0
28	88913.0
29	88913.0
30	114158.5
31	139404.0
32	139404.0
33	139404.0
34	163771.0
35	188138.0
36	188138.0
37	188138.0
38	209853.5
39	231569.0
40	231569.0
41	231569.0
42	247671.5
43	263774.0
44	263774.0
45	263774.0
46	276287.5
47	288801.0
48	288801.0
49	288801.0
50	282359.0
51	275917.0
52	275917.0
53	275917.0
54	250828.5
55	225740.0
56	225740.0
57	225740.0
58	195921.5
59	166103.0
60	166103.0
61	166103.0
62	136838.5
63	107574.0
64	107574.0
65	107574.0
66	83582.5
67	59591.0
68	59591.0
69	59591.0
70	43913.5
71	28236.0
72	28236.0
73	28236.0
74	19985.5
75	11735.0
76	11735.0
77	11735.0
78	8247.5
79	4760.0
80	4760.0
81	4760.0
82	3239.0
83	1718.0
84	1718.0
85	1718.0
86	1138.5
87	559.0
88	559.0
89	559.0
90	361.5
91	164.0
92	164.0
93	164.0
94	97.5
95	31.0
96	31.0
97	31.0
98	18.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18187539274842232
2	0.25629573990803806
3	0.25611118852016546
4	0.45616489297403645
5	0.251128301047606
6	0.01928562003268405
7	0.01854741448119375
8	0.017209416919117586
9	0.0189165172569389
10	0.02076203113566465
11	0.021084996064441655
12	0.028190224497535776
13	0.02643698631274632
14	0.02509898875067015
15	0.04133951088345672
16	0.020623617594760217
17	0.04120109734255229
18	0.03165056302014655
19	0.026759951241523323
20	0.030404841152006673
21	0.034649523073075894
22	0.04535350356968522
23	0.02998960052929338
24	0.04890611778623228
25	0.03704869111541936
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2167418.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.43075347205783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.24449462215058	63.546257052215424
2	10.951323924993476	16.52133230357663
3	2.625866852251345	5.942133455478591
4	0.9185943088061097	2.771610433935561
5	0.4153409486290719	1.5664740351445077
6	0.22544123004269714	1.02031211074729
7	0.14465145899118684	0.7637817979776388
8	0.09321189555535724	0.5624834811439484
9	0.06150590253699026	0.4175492914209716
>10	0.2952533551279003	3.7458968500009395
>50	0.014293164046224287	0.7255749203113256
>100	0.008353725833508553	1.2065227989210807
>500	0.0011124073570598972	0.5309620938960306
>1k	5.562036785293883E-4	0.6791093752301071
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	2476	0.11423730909312371	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2265	0.1045022233828454	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.613784696814366E-5	0.0
5	0.0	4.613784696814366E-5	0.0	4.613784696814366E-5	0.0
6	0.0	4.613784696814366E-5	0.0	4.613784696814366E-5	0.0
7	0.0	4.613784696814366E-5	0.0	4.613784696814366E-5	0.0
8	9.227569393628732E-5	4.613784696814366E-5	0.0	4.613784696814366E-5	0.0
9	9.227569393628732E-5	4.613784696814366E-5	0.0	4.613784696814366E-5	0.0
10	9.227569393628732E-5	4.613784696814366E-5	0.0	9.227569393628732E-5	0.0
11	9.227569393628732E-5	4.613784696814366E-5	0.0	9.227569393628732E-5	0.0
12	9.227569393628732E-5	4.613784696814366E-5	0.0	9.227569393628732E-5	0.0
13	9.227569393628732E-5	4.613784696814366E-5	0.0	9.227569393628732E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTT	40	0.005318921	14.234228	13
GTGCGAA	60	4.131594E-4	12.652647	11
GCGTAGG	55	0.0029453891	12.157509	1
TAGGACC	360	0.0	11.6461115	4
GTCCTAC	935	0.0	10.727214	1
ATTACAC	235	0.0	10.54281	3
TTAGAGT	220	0.0	10.394877	4
TTACACC	215	1.8189894E-12	10.193426	4
ACGAAAT	95	1.6629789E-4	9.988932	12
GTAAGAC	145	1.5471596E-7	9.857667	3
TTACACT	310	0.0	9.836013	4
TTATACT	345	0.0	9.666734	4
TGGCGAG	865	0.0	9.654043	18
AGGACCT	815	0.0	9.568465	5
TCCTACA	1220	0.0	9.535932	2
TAGGACT	320	0.0	9.528637	4
TATGGCG	870	0.0	9.489705	16
GGCGAGG	380	0.0	9.489704	19
TCGAACT	190	1.0732037E-9	9.489704	19
ACCGTGC	140	1.0783133E-6	9.487295	8
>>END_MODULE