FastQCFastQC Report
Thu 26 May 2016
SRR1513129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513129_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2167418
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34810.16060584529610808No Hit
GTACATGGGAAGCAGTGGTATCAAC27450.12664838992755434No Hit
CCCATGTACTCTGCGTTGATACCAC25960.11977385072930095No Hit
TATCAACGCAGAGTACTTTTTTTTT24640.11368365492950598No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGAGT500.001511005613.286962512
TCGAACT1352.910383E-1112.64928619
TAGGACC4650.011.0576834
ATAAGAC1752.2555469E-1010.3387743
CGGTTTC750.002669923210.123413
GTAGGAC10450.010.1148673
TATACTG4150.010.0955025
GGACGTG8850.010.0055956
AACCGAG951.6600825E-49.9906587
GATTGTC1451.639819E-79.8184057
AGGCGTG1554.1240128E-89.7972917
ACTATAC2350.09.7251633
ATTATAC2450.09.7168933
TTACACT3350.09.6640114
AGGACGT9300.09.6242795
TAGGACG9700.09.6200584
GTCCTAA5650.09.6101641
TTTAGAC1304.1134917E-69.5225553
CGGGAGT900.00109067559.52189354
GACGTGG4400.09.4911267
Produced by FastQC (version 0.11.5)