##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513129_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2167418 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.809380562494177 31.0 31.0 34.0 27.0 34.0 2 30.894656683666927 33.0 31.0 34.0 27.0 34.0 3 31.003296087787405 33.0 31.0 34.0 27.0 34.0 4 34.40282538947263 37.0 35.0 37.0 31.0 37.0 5 34.16017491780543 37.0 35.0 37.0 30.0 37.0 6 34.0786770249209 37.0 35.0 37.0 30.0 37.0 7 33.9438862277604 37.0 35.0 37.0 30.0 37.0 8 33.908618918916424 37.0 35.0 37.0 29.0 37.0 9 35.466035162575935 39.0 35.0 39.0 30.0 39.0 10 35.31320677414325 39.0 35.0 39.0 29.0 39.0 11 35.33231384070816 39.0 35.0 39.0 29.0 39.0 12 35.26613648128787 39.0 35.0 39.0 29.0 39.0 13 35.238294597534946 39.0 35.0 39.0 29.0 39.0 14 36.39777744763585 40.0 36.0 41.0 29.0 41.0 15 36.3751145372051 40.0 36.0 41.0 28.0 41.0 16 36.33468348052845 40.0 36.0 41.0 28.0 41.0 17 36.2628247066325 40.0 36.0 41.0 28.0 41.0 18 36.21821540653441 40.0 36.0 41.0 27.0 41.0 19 36.29920393758841 40.0 37.0 41.0 28.0 41.0 20 36.2606972905088 40.0 37.0 41.0 28.0 41.0 21 36.20858090133052 40.0 37.0 41.0 27.0 41.0 22 36.15501070859428 40.0 37.0 41.0 27.0 41.0 23 36.076916404680595 40.0 37.0 41.0 27.0 41.0 24 36.01230127275865 40.0 36.0 41.0 27.0 41.0 25 35.96092631878115 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.410258841912853 1101 2 0.5153945215219125 1101 3 0.4146963763521576 1101 4 0.5656456824088067 1101 5 0.46581199685832075 1101 6 0.3609983228228302 1101 7 0.4261228205129868 1101 8 0.3733432130775398 1101 9 0.3715916975897997 1101 10 0.5571005805009648 1101 11 0.5132018970847554 1101 12 0.4537537484837486 1101 13 0.5454789152347814 1101 14 0.6618682036194841 1101 15 0.6912587720092205 1101 16 0.6123047826557553 1101 17 0.6788494771490292 1101 18 0.5690359941966463 1101 19 0.6392134549444037 1101 20 0.6150046934552691 1101 21 0.5668332496488873 1101 22 0.6888912656935986 1101 23 0.6858187972507039 1101 24 0.6745864876821628 1101 25 0.7263963804644433 1102 1 0.4444046112816551 1102 2 0.5259776489511871 1102 3 0.4327177781047915 1102 4 0.5511330848739533 1102 5 0.4492413290268118 1102 6 0.4960214622636201 1102 7 0.3880877696546676 1102 8 0.49744988493241493 1102 9 0.5148135025056177 1102 10 0.5598134059841513 1102 11 0.5778463189142329 1102 12 0.5378859870591555 1102 13 0.6060966818707598 1102 14 0.5985886923581276 1102 15 0.5466798501120422 1102 16 0.540359084612362 1102 17 0.7187248473678736 1102 18 0.6978170340338892 1102 19 0.8035289310734015 1102 20 0.7362298978772941 1102 21 0.5845880731748494 1102 22 0.6362083093664168 1102 23 0.6529323963835267 1102 24 0.625990563371225 1102 25 0.742679061180354 1103 1 0.5045249125074704 1103 2 0.5045320876196264 1103 3 0.5415043653245739 1103 4 0.5622517249825165 1103 5 0.5295492104349009 1103 6 0.44696093901168865 1103 7 0.44292048995664146 1103 8 0.5064604263528238 1103 9 0.510450937710921 1103 10 0.6215897261645011 1103 11 0.6248162630740026 1103 12 0.5385448721825128 1103 13 0.5519926469872942 1103 14 0.7143831179749256 1103 15 0.5695894564124231 1103 16 0.5573583764128216 1103 17 0.6247623411572221 1103 18 0.5917557173018437 1103 19 0.5361121352499225 1103 20 0.5630308011098037 1103 21 0.49860402805772 1103 22 0.5642528258255695 1103 23 0.46495640101093016 1103 24 0.585604279453662 1103 25 0.6223209198519015 1104 1 0.3207784306844097 1104 2 0.38521380559227225 1104 3 0.3246330075103607 1104 4 0.5399157106085113 1104 5 0.4718286032875838 1104 6 0.43547964873177847 1104 7 0.47199725786351365 1104 8 0.5466066833065 1104 9 0.5280797599391889 1104 10 0.5852966537580357 1104 11 0.5962640985225249 1104 12 0.6325318209402226 1104 13 0.6289562122692303 1104 14 0.549070046156352 1104 15 0.5906253069110292 1104 16 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2216 24 -0.7199540867195253 2216 25 -0.8690532946544351 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48707.0 3 1.0 4 762.0 5 6571.0 6 7544.0 7 5687.0 8 4693.0 9 4215.0 10 3753.0 11 3490.0 12 3077.0 13 2977.0 14 3032.0 15 2963.0 16 3062.0 17 3285.0 18 3779.0 19 4400.0 20 5652.0 21 7507.0 22 9201.0 23 11132.0 24 13184.0 25 14992.0 26 16709.0 27 18527.0 28 20925.0 29 25406.0 30 31804.0 31 40806.0 32 52229.0 33 67836.0 34 89643.0 35 120632.0 36 172853.0 37 279575.0 38 1056807.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.41805097842161 18.775497184995206 13.183809943376316 27.62264189320687 2 14.178072141725352 20.31354251548287 39.031087552151725 26.47729779064005 3 17.032152983327144 24.496849759723528 29.809878212241525 28.661119044707807 4 11.45410344275509 17.00868751565843 37.144170127477494 34.393038914108985 5 13.025631884978834 37.855654640965206 35.75497417786611 13.36373929618985 6 32.50660453970085 37.00519195991625 17.598586586834998 12.889616913547894 7 28.081571882382967 31.303251401768655 22.285825579318484 18.32935113652989 8 25.630309297664912 36.08820384749738 20.238284763166504 18.0432020916712 9 26.47141333195528 14.630803985043169 19.396033043818495 39.50174963918306 10 14.492789732601372 28.706295222776824 34.63318393264327 22.16773111197854 11 34.975994478222475 22.105472963683052 23.95223256427694 18.96629999381753 12 23.064309699375016 24.574355292795392 31.323214995907573 21.03812001192202 13 28.918418136233985 21.257874577031288 25.587173309440082 24.23653397729464 14 21.41842427139358 20.959116283125656 27.338029917075414 30.284429528405347 15 23.745581147706627 29.404065113420668 23.06813914067337 23.78221459819933 16 23.245862127194663 27.547801116351344 26.169986592341672 23.03635016411232 17 22.5071159334148 27.68504195724164 27.057483082352856 22.750359026990708 18 22.533835159028133 27.141970365823546 27.986505165558633 22.33768930958969 19 23.70945521353057 26.674596224632257 27.068659575587173 22.54728898625 20 23.860418248810337 26.6603396299191 27.18561901765142 22.293623103619144 21 23.68437079566154 26.223629743231996 26.780793982109476 23.311205478996992 22 23.68037500893922 26.807048950016494 26.509321011435283 23.003255029609004 23 22.997660673006383 26.7218507906187 26.8497985964038 23.430689939971117 24 23.20835205760956 26.669890164241504 27.121072169742984 23.000685608405945 25 23.346255030188047 26.43685055093305 26.897906906571613 23.31898751230729 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 167.0 1 167.0 2 296.0 3 425.0 4 425.0 5 425.0 6 985.0 7 1545.0 8 1545.0 9 1545.0 10 2230.5 11 2916.0 12 2916.0 13 2916.0 14 5344.5 15 7773.0 16 7773.0 17 7773.0 18 14886.5 19 22000.0 20 22000.0 21 22000.0 22 34545.5 23 47091.0 24 47091.0 25 47091.0 26 67638.0 27 88185.0 28 88185.0 29 88185.0 30 113107.0 31 138029.0 32 138029.0 33 138029.0 34 161899.5 35 185770.0 36 185770.0 37 185770.0 38 206542.0 39 227314.0 40 227314.0 41 227314.0 42 244279.5 43 261245.0 44 261245.0 45 261245.0 46 275091.0 47 288937.0 48 288937.0 49 288937.0 50 283378.5 51 277820.0 52 277820.0 53 277820.0 54 253852.5 55 229885.0 56 229885.0 57 229885.0 58 199799.5 59 169714.0 60 169714.0 61 169714.0 62 140055.0 63 110396.0 64 110396.0 65 110396.0 66 85521.0 67 60646.0 68 60646.0 69 60646.0 70 44656.0 71 28666.0 72 28666.0 73 28666.0 74 20218.5 75 11771.0 76 11771.0 77 11771.0 78 8256.0 79 4741.0 80 4741.0 81 4741.0 82 3205.5 83 1670.0 84 1670.0 85 1670.0 86 1112.0 87 554.0 88 554.0 89 554.0 90 351.0 91 148.0 92 148.0 93 148.0 94 78.5 95 9.0 96 9.0 97 9.0 98 5.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1932714409495538 2 0.008489363842138433 3 0.00567495517708167 4 0.0025375815832479015 5 0.08461681133957548 6 0.34787936613980325 7 0.03188125225498727 8 0.01600983289794585 9 0.005259714554368377 10 0.004244681921069217 11 0.0 12 0.0 13 0.0 14 0.001245721868139879 15 0.0 16 0.0 17 0.02076203113566465 18 0.03058939253987925 19 0.0 20 0.0 21 6.459298575540113E-4 22 1.38413540904431E-4 23 0.005905644411922388 24 0.0 25 1.8455138787257464E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2167418.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.36475197399979 #Duplication Level Percentage of deduplicated Percentage of total 1 82.290682914973 59.549448589130826 2 12.043054038664469 17.429852370348613 3 3.0601513003488283 6.643412695579675 4 1.1081694927803583 3.2076964196081503 5 0.48640554352089516 1.7599308257834063 6 0.28145669540814167 1.2220526372779066 7 0.1706397350784176 0.8643811474101103 8 0.1107974050577258 0.6414261389092094 9 0.07819198430434876 0.5092509195485173 >10 0.34007154446749366 4.238551509330233 >50 0.018119933219761797 0.8931502339097546 >100 0.010282102975863757 1.4271888853114778 >500 0.001339467523049 0.6837735726259692 >1k 6.378416776421686E-4 0.9298840552260924 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3481 0.16060584529610808 No Hit GTACATGGGAAGCAGTGGTATCAAC 2745 0.12664838992755434 No Hit CCCATGTACTCTGCGTTGATACCAC 2596 0.11977385072930095 No Hit TATCAACGCAGAGTACTTTTTTTTT 2464 0.11368365492950598 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 4.613784696814366E-5 0.0 0.0 0.0 0.0 9 4.613784696814366E-5 0.0 0.0 0.0 0.0 10 4.613784696814366E-5 0.0 0.0 4.613784696814366E-5 0.0 11 4.613784696814366E-5 0.0 0.0 4.613784696814366E-5 0.0 12 4.613784696814366E-5 0.0 0.0 4.613784696814366E-5 0.0 13 4.613784696814366E-5 0.0 0.0 4.613784696814366E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGAGT 50 0.0015110056 13.2869625 12 TCGAACT 135 2.910383E-11 12.649286 19 TAGGACC 465 0.0 11.057683 4 ATAAGAC 175 2.2555469E-10 10.338774 3 CGGTTTC 75 0.0026699232 10.1234 13 GTAGGAC 1045 0.0 10.114867 3 TATACTG 415 0.0 10.095502 5 GGACGTG 885 0.0 10.005595 6 AACCGAG 95 1.6600825E-4 9.990658 7 GATTGTC 145 1.639819E-7 9.818405 7 AGGCGTG 155 4.1240128E-8 9.797291 7 ACTATAC 235 0.0 9.725163 3 ATTATAC 245 0.0 9.716893 3 TTACACT 335 0.0 9.664011 4 AGGACGT 930 0.0 9.624279 5 TAGGACG 970 0.0 9.620058 4 GTCCTAA 565 0.0 9.610164 1 TTTAGAC 130 4.1134917E-6 9.522555 3 CGGGAGT 90 0.0010906755 9.5218935 4 GACGTGG 440 0.0 9.491126 7 >>END_MODULE