FastQCFastQC Report
Thu 26 May 2016
SRR1513128_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513128_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166768
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC38340.1769455705456237No Hit
CCCATGTACTCTGCGTTGATACCAC31320.14454708579783346No Hit
GTATCAACGCAGAGTACTTTTTTTT22480.1037489938932087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5150.011.4724044
GCGTTTA752.0974048E-411.38654813
TAGGACT3700.011.074824
GGCGAGG5550.010.60033419
GTCCTAA6900.010.383431
GGGTTAG1201.4196994E-610.3488171
GTAGGAC11750.010.2195743
TACCCTG2800.010.1893835
AGGACGT10700.010.1322445
ACCGTCC750.002677428310.1199748
AGGACCT9600.010.1044715
GTCCTAC12800.010.07517051
TTAGGAC9100.010.0537943
GACGTGA6450.010.00237
GTATAAT3250.09.993651
GTCCTAG2800.09.8939251
TGGCGAG13200.09.84844118
AAGGCGT1554.15439E-89.792626
CGCAGAT1651.0446456E-89.7749758
GTATTAT2251.8189894E-129.7650381
Produced by FastQC (version 0.11.5)