##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513128_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2166768 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.226285878322 31.0 30.0 34.0 23.0 34.0 2 29.09205092561825 31.0 30.0 34.0 18.0 34.0 3 29.285387729558494 31.0 30.0 34.0 22.0 34.0 4 32.33742560347947 35.0 33.0 37.0 19.0 37.0 5 32.474213206028516 37.0 33.0 37.0 22.0 37.0 6 32.82772682631458 37.0 35.0 37.0 26.0 37.0 7 32.77882126743611 37.0 35.0 37.0 25.0 37.0 8 32.65776262156354 37.0 35.0 37.0 25.0 37.0 9 33.873456226047274 39.0 34.0 39.0 22.0 39.0 10 33.44853579155683 38.0 33.0 39.0 17.0 39.0 11 33.892254269954144 39.0 34.0 39.0 23.0 39.0 12 33.87712251611617 39.0 34.0 39.0 23.0 39.0 13 33.92099892558871 39.0 35.0 39.0 23.0 39.0 14 34.117033757190434 39.0 33.0 41.0 13.0 41.0 15 34.53831236200645 39.0 34.0 41.0 17.0 41.0 16 34.38075650000369 39.0 34.0 41.0 17.0 41.0 17 33.99158608581999 39.0 33.0 41.0 11.0 41.0 18 33.71738690990452 39.0 33.0 41.0 11.0 41.0 19 34.34806587507292 39.0 34.0 41.0 18.0 41.0 20 34.55849034137481 39.0 34.0 41.0 18.0 41.0 21 34.602454900570805 40.0 34.0 41.0 18.0 41.0 22 34.28462115002621 40.0 34.0 41.0 10.0 41.0 23 33.818824165762095 39.0 34.0 41.0 8.0 41.0 24 34.0559496909683 39.0 34.0 41.0 2.0 41.0 25 33.70194824734351 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9285477258174026 1101 2 1.1169586465827699 1101 3 1.1033670888744425 1101 4 1.4142924705004702 1101 5 1.2888045325579753 1101 6 1.0529943972471116 1101 7 1.056023245040663 1101 8 1.070088517526706 1101 9 1.4054824343277943 1101 10 1.8084964796288432 1101 11 1.3204585015383117 1101 12 1.314448301715096 1101 13 1.136242184382482 1101 14 2.0194222541608866 1101 15 1.663236929549086 1101 16 1.7987755710695552 1101 17 2.100733991620615 1101 18 2.273345331452994 1101 19 1.777589615044711 1101 20 1.3976403445533663 1101 21 1.3146996691397064 1101 22 1.593596217233383 1101 23 1.9233404986558966 1101 24 1.5220552298927288 1101 25 1.7551223131434597 1102 1 1.1242444832448015 1102 2 1.2634696785963513 1102 3 1.1691136173179828 1102 4 1.481991840238031 1102 5 1.3461969171160604 1102 6 1.1113115876347095 1102 7 1.1718415694048332 1102 8 1.1596367397109688 1102 9 1.48561225963536 1102 10 1.7633800935414854 1102 11 1.3998143950982893 1102 12 1.3151120314695959 1102 13 1.3089868105343214 1102 14 2.366120145371184 1102 15 1.9325083336231899 1102 16 2.087801215015247 1102 17 2.4033758762025244 1102 18 2.516383916095293 1102 19 2.0329159155928522 1102 20 1.7227465858597029 1102 21 1.6287569501602093 1102 22 1.916256419749395 1102 23 2.022634721708606 1102 24 1.8784192303684009 1102 25 2.1257224162568846 1103 1 0.7638383596608342 1103 2 1.068482936054103 1103 3 0.8004518705642738 1103 4 1.2787263980112442 1103 5 1.1532472539177903 1103 6 0.7811535827378719 1103 7 0.898146604008069 1103 8 0.9888064928614924 1103 9 1.2018645874787879 1103 10 1.5852697831854385 1103 11 1.095827465636276 1103 12 1.134227924034576 1103 13 1.1745095743623466 1103 14 1.9566914275715774 1103 15 1.5946156578782222 1103 16 1.6663392485731094 1103 17 2.0088592022667555 1103 18 2.334084064477281 1103 19 1.586982459030935 1103 20 1.4285815980094938 1103 21 1.3608859201861208 1103 22 1.5690887157933915 1103 23 1.9235482055469717 1103 24 1.5179663768419616 1103 25 1.8646779962735494 1104 1 0.9228463237659348 1104 2 1.1831423340641471 1104 3 1.0539782976062355 1104 4 1.397565349836185 1104 5 1.1885412639102881 1104 6 1.014324606633238 1104 7 0.9744583479366185 1104 8 1.1342623623855985 1104 9 1.482302659012376 1104 10 1.6560892380514431 1104 11 1.188337172937871 1104 12 1.1303657829683473 1104 13 1.09363757195878 1104 14 2.0250505046992515 1104 15 1.6603755725942833 1104 16 1.8625401706523803 1104 17 2.230293961269453 1104 18 2.318726053398912 1104 19 1.663278951896487 1104 20 1.4555519590936612 1104 21 1.4438334016255823 1104 22 1.6732889347986557 1104 23 2.0086836426119348 1104 24 1.902443578027885 1104 25 2.158447212612927 1105 1 0.8218243827953877 1105 2 1.0286904180813785 1105 3 1.0256903779679085 1105 4 1.307326030366056 1105 5 1.3296599755974654 1105 6 1.0021828192024245 1105 7 0.9645899967960787 1105 8 1.0348769236117263 1105 9 1.2651966567838215 1105 10 1.663481392860298 1105 11 1.1007367701883055 1105 12 1.072113686484549 1105 13 1.0148231214014913 1105 14 1.6466408721076604 1105 15 1.4637609536806906 1105 16 1.587160628903355 1105 17 1.8513230144967636 1105 18 1.6746359630421281 1105 19 1.3577395893125725 1105 20 1.2546532871197442 1105 21 1.1621455272263645 1105 22 1.395618119189038 1105 23 1.8067267662665216 1105 24 1.5485561452413705 1105 25 1.7987268943299455 1106 1 0.5643694341553065 1106 2 0.7707311036510198 1106 3 0.6455571540842975 1106 4 1.0987140525277468 1106 5 1.009962910358901 1106 6 0.661958957736644 1106 7 0.5869018444282545 1106 8 0.6028453513454508 1106 9 0.8413498856444619 1106 10 0.7115255420857594 1106 11 0.6579491165373739 1106 12 0.6866106325145012 1106 13 0.5883564632175506 1106 14 1.5962131136162014 1106 15 1.006186890262157 1106 16 1.1006225506468041 1106 17 1.5388273543884452 1106 18 1.6733919858734296 1106 19 1.2967878046177432 1106 20 1.0808874960133252 1106 21 0.8547244907344904 1106 22 1.1354671829172247 1106 23 1.5978035360884277 1106 24 1.1548813332783183 1106 25 1.5111597597244284 1107 1 0.7607044367886289 1107 2 0.981407619738139 1107 3 0.908132965321883 1107 4 1.2356168067809747 1107 5 0.9851330670186371 1107 6 0.7998261318607263 1107 7 0.7925154085152428 1107 8 0.8565921009816435 1107 9 1.0115358147117561 1107 10 1.3403977644812954 1107 11 0.8970433087313623 1107 12 0.9410374582693066 1107 13 0.8247916422008856 1107 14 1.5461034683164527 1107 15 0.9322102616853769 1107 16 1.2471102309192759 1107 17 1.6738353094072664 1107 18 1.8025505705957556 1107 19 1.2804171649681635 1107 20 1.1461020429626174 1107 21 0.9754141459069032 1107 22 1.2125030529870884 1107 23 1.5025792999812708 1107 24 1.2835243557083515 1107 25 1.5373032363337416 1108 1 0.4520875708443768 1108 2 0.6639121859603669 1108 3 0.5571017193790198 1108 4 0.9424905051631001 1108 5 0.8419684393680456 1108 6 0.5413912034174686 1108 7 0.48362821510127674 1108 8 0.5323947690426323 1108 9 0.7453454223634424 1108 10 0.9681297170103704 1108 11 0.6774044626739624 1108 12 0.5082913757945278 1108 13 0.4427197777772278 1108 14 1.5351526270111293 1108 15 1.030907354420151 1108 16 1.009135090375672 1108 17 1.3789380071687063 1108 18 1.5810395289635508 1108 19 1.0824414197446899 1108 20 0.7359618283724032 1108 21 0.8508692119681811 1108 22 1.0785857589650547 1108 23 1.2896752537837273 1108 24 1.0782650636461995 1108 25 1.3809416484444128 1109 1 0.46762590187211117 1109 2 0.5376686583582959 1109 3 0.5389156451985642 1109 4 0.7765040782787622 1109 5 0.9095272497492317 1109 6 0.5727711133759001 1109 7 0.3556009280700039 1109 8 0.3434894534923316 1109 9 0.5412752476659008 1109 10 0.8591957838692252 1109 11 0.5216661605102217 1109 12 0.38776379836335195 1109 13 0.4533334543808536 1109 14 1.3931447844536393 1109 15 0.8238553886767832 1109 16 1.0248163333576699 1109 17 1.372954889083303 1109 18 1.5141815503581029 1109 19 1.002005925484049 1109 20 0.8147863951485377 1109 21 0.6713417014169849 1109 22 0.9286878389323903 1109 23 1.29956433861922 1109 24 1.1326726302357812 1109 25 1.296443547483804 1110 1 0.6483301621142452 1110 2 0.8780394991803036 1110 3 0.7234312664377569 1110 4 1.0485067206162455 1110 5 0.9325487907730974 1110 6 0.5866100045780058 1110 7 0.6228691481640709 1110 8 0.646318019549895 1110 9 0.8593624342616621 1110 10 1.226069937275895 1110 11 0.8163962432765217 1110 12 0.6650509399831606 1110 13 0.7194207042240492 1110 14 1.5125650865304365 1110 15 1.237329904350723 1110 16 1.3008647035941294 1110 17 1.599923597412463 1110 18 1.3261354921669408 1110 19 0.9919153235265412 1110 20 0.7481239449778627 1110 21 0.7333508968919773 1110 22 1.0319510554512163 1110 23 -0.09575359396252026 1110 24 0.6703134655939067 1110 25 1.0550907901589568 1111 1 0.35125893991773793 1111 2 0.7349150534297877 1111 3 0.6590554542970146 1111 4 1.010878956086998 1111 5 0.9244066220419995 1111 6 0.6602399952072346 1111 7 0.5522130121292506 1111 8 0.6538104756888785 1111 9 0.8412178989990409 1111 10 1.0793473233768083 1111 11 0.8053747945258038 1111 12 0.70604412038481 1111 13 0.5389089612865874 1111 14 1.4300803671291291 1111 15 1.023132882366255 1111 16 1.1191835646769945 1111 17 1.535016139197353 1111 18 1.6171141526407524 1111 19 1.1155171845045189 1111 20 0.7263764738291698 1111 21 0.6343730998120876 1111 22 0.9719543668616311 1111 23 1.3464794582476713 1111 24 0.9955124319484199 1111 25 1.1603881799527045 1112 1 0.2683807134574785 1112 2 0.5109856626272062 1112 3 0.35915893894090445 1112 4 0.7932936351315973 1112 5 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14 1.5025778626982103 1203 15 1.1669322358783418 1203 16 1.2479827401863446 1203 17 1.731498444598671 1203 18 1.3943204289722004 1203 19 1.1471818435706922 1203 20 1.1124732433273437 1203 21 0.9958448354559408 1203 22 1.2493787979400963 1203 23 1.5793219997301904 1203 24 1.2099179456170006 1203 25 1.1177363807956695 1204 1 0.14534905952538324 1204 2 0.47941297905785873 1204 3 0.413458818384175 1204 4 0.7671547323230214 1204 5 0.482398715473515 1204 6 0.36090296692968593 1204 7 0.12267733779587076 1204 8 0.41764035673579514 1204 9 0.6481411692837682 1204 10 1.0166844271108602 1204 11 0.4357792186404268 1204 12 0.48688989684356443 1204 13 0.3588920244841347 1204 14 0.8047069824550732 1204 15 0.6301725193022989 1204 16 0.6511273468568106 1204 17 1.0337611521363215 1204 18 1.3224211537766948 1204 19 0.7624771284667062 1204 20 0.5640365404763514 1204 21 0.5701855276795413 1204 22 0.758412743283202 1204 23 1.0657166384836998 1204 24 0.6863647858503157 1204 25 0.974926647540272 1205 1 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24 -2.9083119970316105 2216 25 -2.2869029872476965 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 109999.0 3 4.0 4 888.0 5 5307.0 6 6422.0 7 5809.0 8 5421.0 9 5118.0 10 4726.0 11 4520.0 12 4484.0 13 4410.0 14 4411.0 15 4411.0 16 4429.0 17 4904.0 18 5465.0 19 6705.0 20 8520.0 21 10730.0 22 13510.0 23 15607.0 24 18549.0 25 20906.0 26 23482.0 27 26704.0 28 29451.0 29 34969.0 30 41943.0 31 50454.0 32 63795.0 33 82078.0 34 108533.0 35 143577.0 36 202924.0 37 292654.0 38 790949.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.28230638360826 18.00928323100821 12.233754345735631 25.474656039647904 2 14.631554982165895 19.809692424161092 41.023623941938745 24.53512865173427 3 18.586782943960802 24.686389568730995 29.322442285916832 27.404385201391367 4 11.603627130401236 16.549709825858418 38.62047188306232 33.22619116067802 5 12.984668120852264 37.7006715968309 35.991509949942575 13.323150332374261 6 32.23094755170188 36.5650339139996 18.12794906853056 13.076069465767956 7 28.40179655368197 31.19841024386416 22.213656946874263 18.186136255579612 8 25.58900738045023 35.77748143347555 20.742854848068156 17.890656338006064 9 26.743030968855734 14.871651656919177 19.820990874135074 38.56432650009001 10 14.259408771533808 28.36911549307738 35.204355069443196 22.16712066594562 11 34.65686073118907 22.226489597273147 24.159410462136716 18.95723920940107 12 23.419215747827934 24.50367935590497 31.313303849243358 20.76380104702374 13 29.04190649242252 21.40769178212465 25.496963284269462 24.053438441183367 14 20.89074959906712 21.19293804167486 28.384247423365732 29.532064935892286 15 23.791703642688354 29.409581045129368 23.232607726041614 23.566107586140664 16 22.667063654177717 27.441207765260362 27.178872878597986 22.712855701963935 17 22.2826154709219 27.630557691020197 27.681947359065106 22.4048794789928 18 22.120121179328603 26.89993656644411 29.021405824253037 21.95853642997425 19 23.773653717424924 26.770860743715808 27.197424001107954 22.25806153775131 20 24.157247608244727 26.67325919965357 27.181733697676965 21.98775949442474 21 23.76290237615635 26.23241151683513 26.674244463353027 23.330441643655494 22 23.707083234445893 27.005691299413325 26.554025788613895 22.733199677526894 23 22.872980361995214 26.814154062635904 27.240310492653634 23.07255508271525 24 23.475900321008904 26.745231964951294 27.06439386943175 22.714473844608047 25 23.258278133464948 26.402719956489562 27.154456980576818 23.18454492946867 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 182.0 1 182.0 2 309.0 3 436.0 4 436.0 5 436.0 6 959.0 7 1482.0 8 1482.0 9 1482.0 10 2183.0 11 2884.0 12 2884.0 13 2884.0 14 5497.0 15 8110.0 16 8110.0 17 8110.0 18 15110.0 19 22110.0 20 22110.0 21 22110.0 22 35228.5 23 48347.0 24 48347.0 25 48347.0 26 69015.5 27 89684.0 28 89684.0 29 89684.0 30 115467.0 31 141250.0 32 141250.0 33 141250.0 34 165457.5 35 189665.0 36 189665.0 37 189665.0 38 211125.5 39 232586.0 40 232586.0 41 232586.0 42 248879.5 43 265173.0 44 265173.0 45 265173.0 46 280098.5 47 295024.0 48 295024.0 49 295024.0 50 287104.0 51 279184.0 52 279184.0 53 279184.0 54 251177.5 55 223171.0 56 223171.0 57 223171.0 58 192486.0 59 161801.0 60 161801.0 61 161801.0 62 132706.0 63 103611.0 64 103611.0 65 103611.0 66 80640.5 67 57670.0 68 57670.0 69 57670.0 70 42372.0 71 27074.0 72 27074.0 73 27074.0 74 19106.5 75 11139.0 76 11139.0 77 11139.0 78 7662.5 79 4186.0 80 4186.0 81 4186.0 82 2806.5 83 1427.0 84 1427.0 85 1427.0 86 923.0 87 419.0 88 419.0 89 419.0 90 266.5 91 114.0 92 114.0 93 114.0 94 70.5 95 27.0 96 27.0 97 27.0 98 19.5 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17205349165208272 2 0.26491068725401146 3 0.2643107153142376 4 0.4676088995222378 5 0.26075703536326916 6 0.018737585196015448 7 0.01836837169461613 8 0.017029972752043595 9 0.01836837169461613 10 0.021460534768835426 11 0.021275928018135767 12 0.028983259859846556 13 0.028244832857047916 14 0.02630646197470149 15 0.043844103291169154 16 0.020906714516736445 17 0.04444407523094305 18 0.033321518501288554 19 0.02852174298309741 20 0.031429299306617045 21 0.03650598495085768 22 0.04642859780096439 23 0.03055241724079366 24 0.05058224969170672 25 0.039275086211352575 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2166768.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.1133488640354 #Duplication Level Percentage of deduplicated Percentage of total 1 84.31834815775593 62.49115152654963 2 10.709118932556466 15.873773349500075 3 2.6425100473153647 5.8753580704020685 4 0.9166857570360305 2.7175460523961488 5 0.4360032481107611 1.6156830416542713 6 0.27335447054904777 1.215552914360716 7 0.14874989445970865 0.7717046975106585 8 0.10429480740304668 0.6183709956615504 9 0.07721142670455886 0.5150157663280382 >10 0.3465345168544567 4.319306369949476 >50 0.01583714529893869 0.8029298227194358 >100 0.009146354963958452 1.3678134911449629 >500 0.001323144594991567 0.6620164625472872 >1k 8.820963966607105E-4 1.153777439275848 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3834 0.1769455705456237 No Hit CCCATGTACTCTGCGTTGATACCAC 3132 0.14454708579783346 No Hit GTATCAACGCAGAGTACTTTTTTTT 2248 0.1037489938932087 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 4.6151687674914895E-5 0.0 0.0 0.0 5 0.0 4.6151687674914895E-5 0.0 0.0 0.0 6 0.0 4.6151687674914895E-5 0.0 0.0 0.0 7 4.6151687674914895E-5 4.6151687674914895E-5 0.0 0.0 0.0 8 4.6151687674914895E-5 4.6151687674914895E-5 0.0 0.0 0.0 9 4.6151687674914895E-5 4.6151687674914895E-5 0.0 0.0 0.0 10 4.6151687674914895E-5 4.6151687674914895E-5 0.0 0.0 0.0 11 4.6151687674914895E-5 4.6151687674914895E-5 0.0 0.0 0.0 12 4.6151687674914895E-5 4.6151687674914895E-5 0.0 0.0 4.6151687674914895E-5 13 4.6151687674914895E-5 4.6151687674914895E-5 0.0 0.0 4.6151687674914895E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 515 0.0 11.472404 4 GCGTTTA 75 2.0974048E-4 11.386548 13 TAGGACT 370 0.0 11.07482 4 GGCGAGG 555 0.0 10.600334 19 GTCCTAA 690 0.0 10.38343 1 GGGTTAG 120 1.4196994E-6 10.348817 1 GTAGGAC 1175 0.0 10.219574 3 TACCCTG 280 0.0 10.189383 5 AGGACGT 1070 0.0 10.132244 5 ACCGTCC 75 0.0026774283 10.119974 8 AGGACCT 960 0.0 10.104471 5 GTCCTAC 1280 0.0 10.0751705 1 TTAGGAC 910 0.0 10.053794 3 GACGTGA 645 0.0 10.0023 7 GTATAAT 325 0.0 9.99365 1 GTCCTAG 280 0.0 9.893925 1 TGGCGAG 1320 0.0 9.848441 18 AAGGCGT 155 4.15439E-8 9.79262 6 CGCAGAT 165 1.0446456E-8 9.774975 8 GTATTAT 225 1.8189894E-12 9.765038 1 >>END_MODULE