FastQCFastQC Report
Thu 26 May 2016
SRR1513128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513128_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166768
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC44260.20426736964917333No Hit
CCCATGTACTCTGCGTTGATACCAC41210.1901911049083243No Hit
GTATCAACGCAGAGTACTTTTTTTT32210.14865458600090087No Hit
TATCAACGCAGAGTACTTTTTTTTT21930.10121065107108837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG604.020143E-412.69372755
TATGCGA1001.4612306E-712.33838712
GGCGAGG7050.012.2466419
GCGAATC701.10090885E-412.20279915
TGGCGAG13300.012.13087818
GGACGTG13750.011.1466266
AGGACGT13850.011.0669155
CGAATCT951.3750312E-510.989655516
TAGGACG14250.010.8898824
TATGGCG14650.010.88395316
GTAGGAC12950.010.88159753
TGTAGGA13400.010.8724552
TAGGACC5000.010.8531374
GGTCGGT700.001505884810.8449311
GCGCCAC1502.1682354E-910.75654113
ATGGCGA15150.010.71293617
GCGTGCG909.6386124E-510.5424659
GACGTGA8250.010.4696597
GTCCTAC13200.010.4627471
TCCTACA13950.010.4437912
Produced by FastQC (version 0.11.5)