##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513128_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2166768 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.78683643103461 31.0 31.0 34.0 27.0 34.0 2 30.888842737201212 33.0 31.0 34.0 27.0 34.0 3 30.9834633887892 33.0 31.0 34.0 27.0 34.0 4 34.3797836224275 37.0 35.0 37.0 31.0 37.0 5 34.12222028385134 37.0 35.0 37.0 30.0 37.0 6 34.02932432083176 37.0 35.0 37.0 30.0 37.0 7 33.900663107448516 37.0 35.0 37.0 29.0 37.0 8 33.86603318860164 37.0 35.0 37.0 29.0 37.0 9 35.42807905599492 39.0 35.0 39.0 30.0 39.0 10 35.27547942373157 39.0 35.0 39.0 29.0 39.0 11 35.28290476876158 39.0 35.0 39.0 29.0 39.0 12 35.21886145632573 39.0 35.0 39.0 29.0 39.0 13 35.18953759701085 39.0 35.0 39.0 28.0 39.0 14 36.35520369508872 40.0 37.0 41.0 28.0 41.0 15 36.329847034846374 40.0 37.0 41.0 28.0 41.0 16 36.29499928003367 40.0 37.0 41.0 28.0 41.0 17 36.23383075622309 40.0 36.0 41.0 27.0 41.0 18 36.18415031050856 40.0 36.0 41.0 27.0 41.0 19 36.26275309585521 40.0 37.0 41.0 27.0 41.0 20 36.22962126079027 40.0 37.0 41.0 27.0 41.0 21 36.174151085856906 40.0 37.0 41.0 27.0 41.0 22 36.11807863139939 40.0 37.0 41.0 27.0 41.0 23 36.03497190285255 40.0 37.0 41.0 27.0 41.0 24 35.969765567887286 40.0 37.0 41.0 27.0 41.0 25 35.915939777585784 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4008952996608066 1101 2 0.47469144551583753 1101 3 0.4125102981589528 1101 4 0.5481679574021072 1101 5 0.48478748049129194 1101 6 0.3648188787634581 1101 7 0.4942840373225508 1101 8 0.4826272416113966 1101 9 0.5536994437349492 1101 10 0.6301352985726894 1101 11 0.5614632288997043 1101 12 0.5588738883975068 1101 13 0.5543188961835668 1101 14 0.6703756360439286 1101 15 0.7392589372298488 1101 16 0.6199747073916058 1101 17 0.7049889089433989 1101 18 0.6974659609087865 1101 19 0.654397572039926 1101 20 0.6817786602277209 1101 21 0.6548934341267767 1101 22 0.6831556621071897 1101 23 0.6472955567234564 1101 24 0.7431863516798316 1101 25 0.7258956925888427 1102 1 0.5961187114447881 1102 2 0.5801083673514427 1102 3 0.5112871141386677 1102 4 0.6145068457680694 1102 5 0.6786629998835565 1102 6 0.6820431597680852 1102 7 0.6067200901466805 1102 8 0.8163853077581535 1102 9 0.6773586689587745 1102 10 0.8008709172125918 1102 11 0.8014037624322938 1102 12 0.702654467513824 1102 13 0.7240959124249002 1102 14 0.8624409569680154 1102 15 0.8048670837330363 1102 16 0.8988640920926301 1102 17 0.8225800521893305 1102 18 0.8737533196749538 1102 19 0.8958392930038315 1102 20 0.8673103974333429 1102 21 0.9482323530936441 1102 22 0.8733051339704332 1102 23 0.9275863853998345 1102 24 0.9368688723267269 1102 25 0.9172952501539839 1103 1 0.37432975078793973 1103 2 0.4373599313622343 1103 3 0.32931379520507775 1103 4 0.5570267088781833 1103 5 0.3669408226605384 1103 6 0.33064091534625106 1103 7 0.35818717957509705 1103 8 0.3776673004613613 1103 9 0.42768693238180333 1103 10 0.4719025066536986 1103 11 0.4890389042720926 1103 12 0.44188004275364534 1103 13 0.44097765220950436 1103 14 0.4872404797456866 1103 15 0.4920430029283125 1103 16 0.5108376879443952 1103 17 0.6111807154083664 1103 18 0.537155813323146 1103 19 0.4574535875201633 1103 20 0.4870417406480456 1103 21 0.5128119797617856 1103 22 0.474453536729456 1103 23 0.5383000035435117 1103 24 0.5964170110904305 1103 25 0.5115328136432495 1104 1 0.33014780255614795 1104 2 0.5263003180071841 1104 3 0.4086292359913024 1104 4 0.6009795098884183 1104 5 0.49274093155312215 1104 6 0.4011744127810246 1104 7 0.44928654482585983 1104 8 0.5271776650837765 1104 9 0.5380363799883057 1104 10 0.7031334046972901 1104 11 0.6597089749760201 1104 12 0.6365501393843829 1104 13 0.7236829848961861 1104 14 0.7195158849664338 1104 15 0.7155669669236033 1104 16 0.6498884922854558 1104 17 0.6958881695179215 1104 18 0.5656684381530965 1104 19 0.6468706461391491 1104 20 0.7129473487689282 1104 21 0.6510222598933098 1104 22 0.6382242171708867 1104 23 0.6444544556338201 1104 24 0.5871150064907056 1104 25 0.5650665230677916 1105 1 0.26014349876790277 1105 2 0.3757918333960717 1105 3 0.27391421205100386 1105 4 0.37273109857074616 1105 5 0.35005754235403685 1105 6 0.32747659336706647 1105 7 0.3978442254559269 1105 8 0.46952644803014465 1105 9 0.4076067374380301 1105 10 0.4672791672496288 1105 11 0.38282509983597635 1105 12 0.4115736887724353 1105 13 0.4776764183931732 1105 14 0.48185843994410504 1105 15 0.6422282709334866 1105 16 0.41907830225081 1105 17 0.571740198143047 1105 18 0.6371625585862972 1105 19 0.5684857204800693 1105 20 0.5824571723363832 1105 21 0.5550112529738485 1105 22 0.5348950623832849 1105 23 0.6089319780180773 1105 24 0.6376611777131629 1105 25 0.6837342804829092 1106 1 0.21751722340275848 1106 2 0.25743299369825223 1106 3 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15 0.48914685396028545 1107 16 0.5529519358112154 1107 17 0.48798667472076573 1107 18 0.4947141875556227 1107 19 0.4889927162921879 1107 20 0.5049193083337045 1107 21 0.6295626617220975 1107 22 0.5668650019765025 1107 23 0.46680469759205323 1107 24 0.5360122781519081 1107 25 0.5771172118323236 1108 1 0.21423618915281395 1108 2 0.29794772296120087 1108 3 0.3497386049353146 1108 4 0.3427624515277756 1108 5 0.2295524574566059 1108 6 0.22011945230276808 1108 7 0.25471792203831 1108 8 0.41830861536741537 1108 9 0.39511138909063703 1108 10 0.4870139057367169 1108 11 0.41417205473595686 1108 12 0.4082015816638389 1108 13 0.4500723386892176 1108 14 0.4239465927169235 1108 15 0.45086119252054147 1108 16 0.48067464029527684 1108 17 0.4664682524372452 1108 18 0.47832567362753764 1108 19 0.46345495136682047 1108 20 0.46268161934901286 1108 21 0.5073966095168885 1108 22 0.3401826136333099 1108 23 0.362493152297084 1108 24 0.3569676367715289 1108 25 0.35815542937835687 1109 1 0.07803282473788897 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-1.172867832692532 2215 19 -1.1982556297334739 2215 20 -1.0977891056712181 2215 21 -1.153242624376574 2215 22 -1.3037707066592432 2215 23 -1.2885383262602588 2215 24 -1.1760084498946668 2215 25 -1.1235570371359103 2216 1 -0.6367468301342143 2216 2 -0.5664024682076949 2216 3 -0.6306246395954602 2216 4 -0.4227831950673391 2216 5 -0.6835639972465302 2216 6 -0.6969905182539122 2216 7 -0.9465330645255179 2216 8 -0.8816360897976878 2216 9 -1.0166319105459252 2216 10 -0.9483509220222359 2216 11 -0.9803282359163816 2216 12 -0.9562830657488846 2216 13 -1.03356582514418 2216 14 -1.1773700860011829 2216 15 -1.1362985906480887 2216 16 -1.0793526290378352 2216 17 -1.286959318418127 2216 18 -1.0355875938246015 2216 19 -1.156617546305597 2216 20 -1.1404269428434972 2216 21 -1.3874352138421102 2216 22 -1.3204857449607843 2216 23 -1.293825345951916 2216 24 -1.354232639230041 2216 25 -1.454789942160403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51236.0 3 0.0 4 855.0 5 7025.0 6 8083.0 7 6185.0 8 4915.0 9 4361.0 10 3893.0 11 3610.0 12 3367.0 13 3274.0 14 3135.0 15 3192.0 16 3195.0 17 3416.0 18 3824.0 19 4476.0 20 5651.0 21 7334.0 22 9413.0 23 11334.0 24 12991.0 25 14738.0 26 16282.0 27 18090.0 28 20054.0 29 24498.0 30 30858.0 31 39729.0 32 50363.0 33 65861.0 34 87054.0 35 117155.0 36 167280.0 37 273480.0 38 1076561.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.25323888698804 18.8432018050842 13.27754094267563 27.62601836525213 2 13.986848342380656 20.18932220545738 39.414678771201125 26.409150680960835 3 17.06743861035367 24.515750568157692 29.627508247748143 28.7893025737405 4 11.41198672828381 16.942345386767883 37.15928228611727 34.48638559883104 5 13.000053116678867 37.90951227341695 35.66738797576956 13.423046634134625 6 32.437667153154884 36.82541431757062 17.763611174906096 12.973307354368394 7 28.07479274250212 31.32037268032718 22.23751307621924 18.36732150095146 8 25.60555390150964 36.192955610793 20.217872466136296 17.983618021561064 9 26.441792232379687 14.648853697001977 19.51168179978317 39.39767227083517 10 14.666789126829807 28.653232048316994 34.48959727067681 22.19038155417639 11 34.60896598066798 22.12368836903628 24.127040827628985 19.140304822666756 12 23.200638000930418 24.543190595393693 31.309581828788314 20.946589574887575 13 28.918185980224926 21.303480575677693 25.536282610782514 24.242050833314874 14 21.351730923930177 21.264456672788018 27.23935067218711 30.144461731094697 15 23.65477060765158 29.515065756924596 23.10658086144894 23.72358277397488 16 22.860453911078622 27.48642217348604 26.73710337239612 22.916020543039217 17 22.407691895794365 27.922359084387804 27.05033208206314 22.619616937754692 18 22.426881159984987 27.036924015868173 28.366642860407453 22.16955196373939 19 23.73710521846363 26.725519298789717 27.115316452892053 22.422059029854605 20 23.983186017146274 26.586233505386826 27.25838668468429 22.172193792782615 21 23.53396995412029 26.25190780859655 26.80757797496992 23.40654426231324 22 23.694575233320073 26.987824250518976 26.436957197962307 22.880643318198643 23 22.824505168128603 26.82926829268293 26.950561813310443 23.395664725878028 24 23.33286258611905 26.71125842729817 27.198851007583645 22.757027978999137 25 23.240339178139596 26.45245197107026 26.940275534932923 23.366933315857217 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 168.0 1 168.0 2 302.0 3 436.0 4 436.0 5 436.0 6 928.0 7 1420.0 8 1420.0 9 1420.0 10 2080.5 11 2741.0 12 2741.0 13 2741.0 14 5117.0 15 7493.0 16 7493.0 17 7493.0 18 14301.5 19 21110.0 20 21110.0 21 21110.0 22 34223.5 23 47337.0 24 47337.0 25 47337.0 26 67896.5 27 88456.0 28 88456.0 29 88456.0 30 113634.5 31 138813.0 32 138813.0 33 138813.0 34 162264.5 35 185716.0 36 185716.0 37 185716.0 38 207086.0 39 228456.0 40 228456.0 41 228456.0 42 245222.0 43 261988.0 44 261988.0 45 261988.0 46 278976.0 47 295964.0 48 295964.0 49 295964.0 50 289409.0 51 282854.0 52 282854.0 53 282854.0 54 254741.0 55 226628.0 56 226628.0 57 226628.0 58 196356.5 59 166085.0 60 166085.0 61 166085.0 62 136576.5 63 107068.0 64 107068.0 65 107068.0 66 82890.0 67 58712.0 68 58712.0 69 58712.0 70 43322.5 71 27933.0 72 27933.0 73 27933.0 74 19554.5 75 11176.0 76 11176.0 77 11176.0 78 7671.0 79 4166.0 80 4166.0 81 4166.0 82 2812.5 83 1459.0 84 1459.0 85 1459.0 86 954.0 87 449.0 88 449.0 89 449.0 90 288.0 91 127.0 92 127.0 93 127.0 94 69.5 95 12.0 96 12.0 97 12.0 98 6.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1840529304475606 2 0.009091882471958234 3 0.005353595770290128 4 0.0025383428221203196 5 0.07951935786387836 6 0.3263847352369982 7 0.028706349733797067 8 0.01675306262599411 9 0.004938230581215894 10 0.003553679950968447 11 0.0 12 0.0 13 0.0 14 0.0016153090686220214 15 0.0 16 0.0 17 0.020122135826262894 18 0.030967782429867897 19 0.0 20 0.0 21 6.922753151237235E-4 22 9.23033753498298E-5 23 0.005676657584014532 24 0.0 25 4.61516876749149E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2166768.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.8827443481693 #Duplication Level Percentage of deduplicated Percentage of total 1 82.42672532783823 58.426324988699285 2 11.78192988865806 16.702710484516082 3 2.9655051184779957 6.306094235287898 4 1.153132165800628 3.2694869003238676 5 0.5157613887110565 1.8279291330331293 6 0.3091870841810145 1.3149617426255251 7 0.18447183660334254 0.9153109023374393 8 0.12555522919128492 0.7119759369873322 9 0.08757100538360872 0.5586545868226646 >10 0.4155674758295884 5.019226706554869 >50 0.020370227513795594 0.9851718280274089 >100 0.011811445012128012 1.724530075939474 >500 0.0012384953833803523 0.6392349004691938 >1k 0.0011733114158339144 1.5983875783758463 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 4426 0.20426736964917333 No Hit CCCATGTACTCTGCGTTGATACCAC 4121 0.1901911049083243 No Hit GTATCAACGCAGAGTACTTTTTTTT 3221 0.14865458600090087 No Hit TATCAACGCAGAGTACTTTTTTTTT 2193 0.10121065107108837 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.6151687674914895E-5 7 0.0 0.0 0.0 0.0 4.6151687674914895E-5 8 0.0 0.0 0.0 0.0 4.6151687674914895E-5 9 0.0 0.0 0.0 0.0 4.6151687674914895E-5 10 0.0 0.0 0.0 0.0 4.6151687674914895E-5 11 0.0 0.0 0.0 0.0 4.6151687674914895E-5 12 0.0 0.0 0.0 0.0 9.230337534982979E-5 13 0.0 0.0 0.0 0.0 1.3845506302474469E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 60 4.020143E-4 12.6937275 5 TATGCGA 100 1.4612306E-7 12.338387 12 GGCGAGG 705 0.0 12.24664 19 GCGAATC 70 1.10090885E-4 12.202799 15 TGGCGAG 1330 0.0 12.130878 18 GGACGTG 1375 0.0 11.146626 6 AGGACGT 1385 0.0 11.066915 5 CGAATCT 95 1.3750312E-5 10.9896555 16 TAGGACG 1425 0.0 10.889882 4 TATGGCG 1465 0.0 10.883953 16 GTAGGAC 1295 0.0 10.8815975 3 TGTAGGA 1340 0.0 10.872455 2 TAGGACC 500 0.0 10.853137 4 GGTCGGT 70 0.0015058848 10.84493 11 GCGCCAC 150 2.1682354E-9 10.756541 13 ATGGCGA 1515 0.0 10.712936 17 GCGTGCG 90 9.6386124E-5 10.542465 9 GACGTGA 825 0.0 10.469659 7 GTCCTAC 1320 0.0 10.462747 1 TCCTACA 1395 0.0 10.443791 2 >>END_MODULE