FastQCFastQC Report
Thu 26 May 2016
SRR1513127_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513127_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028803
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC55460.18310864060818746No Hit
CCCATGTACTCTGCGTTGATACCAC45940.15167708167219857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5700.011.3673144
GTCCTAC14850.010.932871
TACCGAG700.001508905910.8425837
GTATAAT4950.010.8042481
GTATACG803.593582E-410.7439571
TCCTACA17750.010.1525582
GTAGGAC13500.010.0931353
CGCAGAT2350.010.092838
GTCCTAT3800.010.0528261
AGGACGT12600.010.0378685
GGACGTG12650.09.8992596
TGGACCG1252.5391546E-69.889945
CCTACAG16550.09.6148453
TGTAGGA16350.09.5639692
GTCCTAA9100.09.5501831
AGCACCG1401.0450003E-69.5095595
AGAACCG1606.539449E-89.5095595
TAGGACG12850.09.4914084
AATCCCG1106.917799E-59.49023819
ACAACGT800.00456781649.487268
Produced by FastQC (version 0.11.5)