##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513127_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3028803 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.052261569999764 31.0 30.0 34.0 22.0 34.0 2 28.92053032171455 31.0 30.0 34.0 16.0 34.0 3 29.107065398442884 31.0 30.0 34.0 20.0 34.0 4 32.15106231735771 35.0 33.0 37.0 19.0 37.0 5 32.279936001119914 35.0 33.0 37.0 19.0 37.0 6 32.62920764407589 37.0 33.0 37.0 25.0 37.0 7 32.570441524258925 37.0 33.0 37.0 24.0 37.0 8 32.45313610690428 37.0 33.0 37.0 22.0 37.0 9 33.64490823602591 39.0 34.0 39.0 18.0 39.0 10 33.219127820462404 38.0 33.0 39.0 17.0 39.0 11 33.66406332798799 38.0 34.0 39.0 22.0 39.0 12 33.64717348734797 38.0 34.0 39.0 20.0 39.0 13 33.68569365521627 39.0 34.0 39.0 21.0 39.0 14 33.87678465717315 39.0 33.0 41.0 10.0 41.0 15 34.29095586606326 39.0 33.0 41.0 17.0 41.0 16 34.1326626393331 39.0 33.0 41.0 16.0 41.0 17 33.74074675705221 39.0 33.0 41.0 10.0 41.0 18 33.46352073740022 39.0 32.0 41.0 10.0 41.0 19 34.094116388553495 39.0 34.0 41.0 16.0 41.0 20 34.30361928458206 39.0 34.0 41.0 17.0 41.0 21 34.342290006976356 39.0 34.0 41.0 16.0 41.0 22 34.02740488569247 39.0 34.0 41.0 9.0 41.0 23 33.561937834847626 39.0 33.0 41.0 2.0 41.0 24 33.79871784331962 39.0 34.0 41.0 2.0 41.0 25 33.435691591694805 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.002419400950302 1101 2 1.1783748614433556 1101 3 1.154331007191761 1101 4 1.4817179474966409 1101 5 1.2602449637967297 1101 6 1.0079391278149927 1101 7 0.9220286763139285 1101 8 1.005649892483035 1101 9 1.3027037644308948 1101 10 1.7229059751917006 1101 11 1.3153416331236087 1101 12 1.1551070572370605 1101 13 1.15917582044694 1101 14 2.1167485805539243 1101 15 1.6445020473203797 1101 16 1.7007369377138986 1101 17 2.12549162486642 1101 18 2.2183117346747636 1101 19 1.7094948591932635 1101 20 1.2883930134555968 1101 21 1.2974547546464095 1101 22 1.5726857948848973 1101 23 1.8028170478812626 1101 24 1.5384433845131653 1101 25 1.7645053969264808 1102 1 1.017354619529339 1102 2 1.3042186524815627 1102 3 1.1878383154052763 1102 4 1.4755224709170633 1102 5 1.4282773415459573 1102 6 1.2044018653298636 1102 7 1.1972632611155163 1102 8 1.2675840305903776 1102 9 1.609820261783213 1102 10 1.7856102872058912 1102 11 1.4891122587437522 1102 12 1.2846754145836385 1102 13 1.2700518519003055 1102 14 2.223673361743238 1102 15 1.7927368080640136 1102 16 1.9036665107041983 1102 17 2.1928073834827586 1102 18 2.4599413882454186 1102 19 1.8778554047128893 1102 20 1.657480271335345 1102 21 1.569715973985474 1102 22 1.677698378326383 1102 23 2.0510133661146526 1102 24 1.7972613076579265 1102 25 2.1743174477985505 1103 1 0.8313489387089241 1103 2 1.1378650736015423 1103 3 0.914809637454745 1103 4 1.3808227279416414 1103 5 1.3434726397030659 1103 6 1.0334656597396759 1103 7 1.0105597211023252 1103 8 0.9988480683937411 1103 9 1.3689149654558506 1103 10 1.6863876975328012 1103 11 1.310329251101784 1103 12 1.2059872837651255 1103 13 1.209986054589919 1103 14 2.006803903950896 1103 15 1.6341740826456004 1103 16 1.7038582291830764 1103 17 2.0783680053029556 1103 18 2.3197095135132813 1103 19 1.682572966258185 1103 20 1.31398205994644 1103 21 1.4475404629142616 1103 22 1.6766186540414267 1103 23 2.0620115621533017 1103 24 1.8359783215063104 1103 25 2.034769874918638 1104 1 1.0192938511367977 1104 2 1.2408203278689776 1104 3 1.1226070840239117 1104 4 1.5433877925946646 1104 5 1.43971753638683 1104 6 1.283636982137054 1104 7 1.1386323411271562 1104 8 1.224839076221329 1104 9 1.512905437262475 1104 10 1.6784814122662866 1104 11 1.29511178402921 1104 12 1.311111956714214 1104 13 1.2206201337455482 1104 14 2.2122883359068837 1104 15 1.8301136877447703 1104 16 1.9260642078600156 1104 17 2.254719481223475 1104 18 2.411148489448827 1104 19 1.7820404306266084 1104 20 1.6400250594044081 1104 21 1.6250469558588563 1104 22 1.8589135208594456 1104 23 2.0675555092626965 1104 24 1.9000796991763522 1104 25 2.116065651530093 1105 1 0.7659345740680408 1105 2 0.995323637137453 1105 3 0.8489532838635832 1105 4 1.2115409599858395 1105 5 1.1520914242334541 1105 6 0.8537971514523832 1105 7 0.8048138647487377 1105 8 0.9188480683937428 1105 9 1.1996331975000558 1105 10 1.4817468135548992 1105 11 0.998064057731618 1105 12 1.041125405312087 1105 13 0.9626932369103613 1105 14 1.663820478536529 1105 15 1.260030436236761 1105 16 1.4810957982438495 1105 17 1.716821043976985 1105 18 1.271974706883448 1105 19 1.2069928557609444 1105 20 1.0726560930956097 1105 21 1.0291979214778024 1105 22 1.2288838474115948 1105 23 1.555934214087003 1105 24 1.3528843988543784 1105 25 1.5419521953606221 1106 1 0.6919548230833463 1106 2 0.9106341132272249 1106 3 0.7869698608523805 1106 4 1.2511429969031624 1106 5 1.1574707508203232 1106 6 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1107 19 1.130852898965749 1107 20 0.9255201317887654 1107 21 0.7564713352306924 1107 22 1.040456903673423 1107 23 1.3646267430973111 1107 24 1.1232137876784805 1107 25 1.406387617563304 1108 1 0.8120111850105225 1108 2 1.1291760958614923 1108 3 0.8650220598932705 1108 4 1.3520646745667477 1108 5 1.2207513212501269 1108 6 0.8034982477457149 1108 7 0.8569166293197128 1108 8 0.8554862844589977 1108 9 1.0754781970066531 1108 10 1.300935573130083 1108 11 0.9811668496475079 1108 12 0.8057275308442726 1108 13 0.8065595702068364 1108 14 1.851030334119713 1108 15 1.3548409181373984 1108 16 1.4521625947394199 1108 17 1.7086070593115394 1108 18 1.9948167709727969 1108 19 1.3029216258223073 1108 20 1.1150066144918185 1108 21 1.1506454424234107 1108 22 1.3735890989432065 1108 23 1.6010248862607028 1108 24 1.327783763620097 1108 25 1.6775783642071573 1109 1 0.7690890676694693 1109 2 0.9992089856559154 1109 3 0.9417257000604273 1109 4 1.198662897879423 1109 5 1.2538227360231247 1109 6 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 165736.0 3 4.0 4 1311.0 5 7937.0 6 9596.0 7 8567.0 8 7863.0 9 7354.0 10 6977.0 11 6696.0 12 6543.0 13 6400.0 14 6463.0 15 6305.0 16 6391.0 17 6965.0 18 7847.0 19 9587.0 20 12270.0 21 15583.0 22 19197.0 23 23215.0 24 26255.0 25 29893.0 26 33395.0 27 37780.0 28 41864.0 29 50030.0 30 60043.0 31 71281.0 32 90216.0 33 116639.0 34 152602.0 35 202033.0 36 284074.0 37 405101.0 38 1078790.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.43798033624902 17.900019612817193 12.302958161858992 25.359041889074803 2 14.497083628898439 19.593128082331667 41.225878825533776 24.683909463236116 3 18.656843390308072 24.425651475609968 29.301076336615704 27.61642879746626 4 11.49958987724842 16.407032817448773 38.74169099265627 33.35168631264654 5 12.966283439426144 37.820765106184936 36.028899436198174 13.184052018190743 6 32.502779648121475 36.6353824851953 17.984513223926207 12.877324642757015 7 28.610564503266385 31.316562278235814 21.90228556011958 18.170587658378224 8 25.54698897667541 36.26938625092709 20.425511820800143 17.75811295159736 9 26.61020100248624 14.980858714428132 19.726272846782866 38.68266743630276 10 13.980120909832122 28.674856111223573 35.29091637394669 22.05410660499761 11 34.86664434330686 22.37613012009043 23.80855671553369 18.948668821069028 12 23.125327151106244 24.642317542564733 31.3970281056857 20.835327200643324 13 29.009565672561067 21.33780711125936 25.493466842139156 24.159160374040418 14 20.774734634002645 21.098636034294977 28.436845501541903 29.689783830160476 15 23.78776984001419 29.406711166439475 23.2339283408107 23.571590652735633 16 22.718018844777433 27.662420047731125 27.040270945273665 22.579290162217784 17 22.141836541237257 27.831343765709505 27.680730711124824 22.346088981928418 18 22.087299763005003 27.162546335560613 28.909889058863 21.840264842571383 19 23.62781926952869 26.917829820891036 27.214985246256244 22.239365663324026 20 23.86262984669058 26.834498351053536 27.29001444520696 22.012857357048922 21 23.61323400606902 26.561577311320693 26.758351509130613 23.066837173479673 22 23.469213166649492 27.093093771552233 26.722560451959488 22.715132609838786 23 22.768129453660137 26.964118406056564 27.267785495776568 22.99996664450673 24 23.128663199674442 26.979977591391712 27.2301255727672 22.661233636166646 25 23.232563431599758 26.533685141203694 27.11232519835431 23.121426228842235 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 182.0 1 182.0 2 367.0 3 552.0 4 552.0 5 552.0 6 1180.0 7 1808.0 8 1808.0 9 1808.0 10 3004.5 11 4201.0 12 4201.0 13 4201.0 14 8023.0 15 11845.0 16 11845.0 17 11845.0 18 22084.0 19 32323.0 20 32323.0 21 32323.0 22 50285.5 23 68248.0 24 68248.0 25 68248.0 26 97126.0 27 126004.0 28 126004.0 29 126004.0 30 161787.5 31 197571.0 32 197571.0 33 197571.0 34 230965.5 35 264360.0 36 264360.0 37 264360.0 38 295726.0 39 327092.0 40 327092.0 41 327092.0 42 349715.5 43 372339.0 44 372339.0 45 372339.0 46 392961.0 47 413583.0 48 413583.0 49 413583.0 50 402583.0 51 391583.0 52 391583.0 53 391583.0 54 351814.0 55 312045.0 56 312045.0 57 312045.0 58 268829.5 59 225614.0 60 225614.0 61 225614.0 62 184078.5 63 142543.0 64 142543.0 65 142543.0 66 110367.5 67 78192.0 68 78192.0 69 78192.0 70 57173.0 71 36154.0 72 36154.0 73 36154.0 74 25268.5 75 14383.0 76 14383.0 77 14383.0 78 9930.0 79 5477.0 80 5477.0 81 5477.0 82 3689.5 83 1902.0 84 1902.0 85 1902.0 86 1241.0 87 580.0 88 580.0 89 580.0 90 377.0 91 174.0 92 174.0 93 174.0 94 103.0 95 32.0 96 32.0 97 32.0 98 24.0 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1739961298242243 2 0.2560087268798928 3 0.25802272382852237 4 0.4573423890560066 5 0.2544239423957253 6 0.017894858133724774 7 0.017498662012682898 8 0.01640912267981774 9 0.01756469469952321 10 0.01967774067841322 11 0.019314560900791502 12 0.025356551746680125 13 0.026016878615083255 14 0.023804783605932772 15 0.040345971659431136 16 0.019479642617892284 17 0.0388602362055241 18 0.029417561987359363 19 0.02502638831247856 20 0.02671022182690654 21 0.031926804087291254 22 0.042095837860699425 23 0.026941336230847632 24 0.04605779907111819 25 0.0334785722280386 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3028803.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.7557881453592 #Duplication Level Percentage of deduplicated Percentage of total 1 80.55352567696748 56.19074671484297 2 12.519301880382338 17.465875393914953 3 3.639944047385666 7.617214974911876 4 1.4105051179153982 3.9356358473300572 5 0.6618318227298983 2.308330020710186 6 0.3481833953015098 1.4572684295030387 7 0.2110774695769912 1.0306712675049772 8 0.13574594138841642 0.757525210326618 9 0.09503448146785262 0.5966284640198035 >10 0.39561941213582547 4.59496430900039 >50 0.017725452816726973 0.8509454397934534 >100 0.009494956027526731 1.343281801504553 >500 0.0010530383314546609 0.5209999610649977 >1k 9.094421942943777E-4 1.1447374807722281 >5k 4.786537864707251E-5 0.18517468479984767 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 5546 0.18310864060818746 No Hit CCCATGTACTCTGCGTTGATACCAC 4594 0.15167708167219857 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.3111440394109488E-4 0.0 0.0 0.0 0.0 7 2.3111440394109488E-4 0.0 0.0 9.904903026046924E-5 0.0 8 2.3111440394109488E-4 0.0 0.0 1.3206537368062564E-4 0.0 9 2.3111440394109488E-4 0.0 0.0 2.3111440394109488E-4 0.0 10 2.3111440394109488E-4 0.0 0.0 2.3111440394109488E-4 0.0 11 2.3111440394109488E-4 0.0 0.0 2.3111440394109488E-4 0.0 12 2.3111440394109488E-4 3.301634342015641E-5 0.0 2.3111440394109488E-4 1.3206537368062564E-4 13 2.3111440394109488E-4 3.301634342015641E-5 0.0 2.3111440394109488E-4 1.3206537368062564E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 570 0.0 11.367314 4 GTCCTAC 1485 0.0 10.93287 1 TACCGAG 70 0.0015089059 10.842583 7 GTATAAT 495 0.0 10.804248 1 GTATACG 80 3.593582E-4 10.743957 1 TCCTACA 1775 0.0 10.152558 2 GTAGGAC 1350 0.0 10.093135 3 CGCAGAT 235 0.0 10.09283 8 GTCCTAT 380 0.0 10.052826 1 AGGACGT 1260 0.0 10.037868 5 GGACGTG 1265 0.0 9.899259 6 TGGACCG 125 2.5391546E-6 9.88994 5 CCTACAG 1655 0.0 9.614845 3 TGTAGGA 1635 0.0 9.563969 2 GTCCTAA 910 0.0 9.550183 1 AGCACCG 140 1.0450003E-6 9.509559 5 AGAACCG 160 6.539449E-8 9.509559 5 TAGGACG 1285 0.0 9.491408 4 AATCCCG 110 6.917799E-5 9.490238 19 ACAACGT 80 0.0045678164 9.48726 8 >>END_MODULE