FastQCFastQC Report
Thu 26 May 2016
SRR1513127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028803
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC64720.2136817746152523No Hit
CCCATGTACTCTGCGTTGATACCAC59170.19535770401706548No Hit
GTATCAACGCAGAGTACTTTTTTTT42470.14022041050540426No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAG550.003089093412.08069313
TAGGACC8200.011.6087464
GTAGGAC15550.011.4482513
TGGCGAG15250.011.32812718
GTCCTAC15300.010.8298491
GGACGTG13250.010.6318546
TATGGCG17100.010.60197116
AGGACGT13800.010.5538645
ATGGCGA17500.010.46863617
TAGGACG14750.010.2613444
TGTAGGA18750.010.2064112
GGCGAGG6800.010.18604419
GGCGAGA10250.010.1826419
CTGTAGG18200.010.0983521
AAGGCGT1909.640644E-1110.019356
AGGACCT14750.010.0031985
GACGTGG7050.09.8277577
CTAGGAC4650.09.8268933
AGGACCG1659.900759E-99.8076315
TGTACCG1752.4883775E-99.7911475
Produced by FastQC (version 0.11.5)