FastQCFastQC Report
Thu 26 May 2016
SRR1513126_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513126_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747569
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC24280.1389358588988475No Hit
CCCATGTACTCTGCGTTGATACCAC21550.12331415812480079No Hit
GTCCTACAGTGGACATTTCTAAATT19700.11272802390062996No Hit
GTATCAACGCAGAGTACTTTTTTTT19160.10963801715411522No Hit
GTCCTAAAGTGTGTATTTCTCATTT18280.10460245060423938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4850.012.5717734
GGCCGCT550.003094453812.07742712
TTAGGAC7650.011.2082913
GTCCTAC11900.011.1548431
GGACGTG9200.011.13750656
AGGACGT9350.011.0901415
GTAGGAC11150.010.8514353
TCCTACA12900.010.7893722
GACGTGG4900.010.6495477
CTCCTAG1451.33022695E-810.5377441
TGTAGGA11950.010.4504842
GGCGAGG5100.010.42093519
GTCTTAT1951.4551915E-1110.2844321
CTTATAC1303.694022E-710.2598963
TAGGACA4850.010.2145654
GACGTGA5400.010.1905757
ATAAGAC2250.010.1621843
GGACCTG8500.010.1572126
TAGGACG10300.010.0820214
GTCCTAA7500.010.05915451
Produced by FastQC (version 0.11.5)