FastQCFastQC Report
Thu 26 May 2016
SRR1513126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747569
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC29710.17000759340546784No Hit
CCCATGTACTCTGCGTTGATACCAC27650.15821978989098573No Hit
GTATCAACGCAGAGTACTTTTTTTT25390.14528753943335No Hit
GTCCTAAAGTGTGTATTTCTCATTT20450.11701969993745598No Hit
CTTTAGGACGTGAAATATGGCGAGG19830.11347191441367979No Hit
GTCCTACAGTGGACATTTCTAAATT19490.11152635461031868No Hit
TATCAACGCAGAGTACTTTTTTTTT19300.11043913001432275No Hit
CTGTAGGACGTGGAATATGGCAAGA19220.10998135123706132No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAA500.001510976213.28675818
TAGGACC5100.012.8806944
AGGACGT10600.011.5862855
TTTATAC2500.011.4249433
GGACGTG10450.011.2053196
GTAGGAC12950.011.1749763
TGTAGGA13400.011.1559122
CCACGAC600.00578131911.1075833
TATCCGT600.00578502711.1066276
CCGCTCC600.005928713411.0700819
GTCCCGT700.001460054510.8849611
GACGTGG5000.010.8198377
TCTATAC1951.8189894E-1210.7413993
GTCCTAT1605.2023097E-1010.7148841
ATAGGAC2400.010.7108843
GGACCGT803.69871E-410.7099626
GGCGAGG5100.010.60586219
TGTGCGC909.634618E-510.54263210
TAGGACA4250.010.5285674
AGGACCT8800.010.4942025
Produced by FastQC (version 0.11.5)