##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513126_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1747569 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.84764321179879 31.0 31.0 34.0 27.0 34.0 2 30.94392839424366 31.0 31.0 34.0 27.0 34.0 3 31.04716208630389 33.0 31.0 34.0 27.0 34.0 4 34.4638454905071 37.0 35.0 37.0 31.0 37.0 5 34.25159922154719 37.0 35.0 37.0 30.0 37.0 6 34.177607865554954 37.0 35.0 37.0 30.0 37.0 7 34.03666121337698 37.0 35.0 37.0 30.0 37.0 8 34.00078737949689 37.0 35.0 37.0 30.0 37.0 9 35.554270532379554 39.0 35.0 39.0 30.0 39.0 10 35.39397357128674 39.0 35.0 39.0 29.0 39.0 11 35.428329868520215 39.0 35.0 39.0 30.0 39.0 12 35.3715733112684 39.0 35.0 39.0 30.0 39.0 13 35.34937447391204 39.0 35.0 39.0 30.0 39.0 14 36.50368426082175 40.0 36.0 41.0 29.0 41.0 15 36.49013057567398 40.0 37.0 41.0 29.0 41.0 16 36.450629989431036 40.0 36.0 41.0 29.0 41.0 17 36.37862482110864 40.0 36.0 41.0 29.0 41.0 18 36.33703619141791 40.0 36.0 41.0 29.0 41.0 19 36.41750797822575 40.0 37.0 41.0 29.0 41.0 20 36.38198262843985 40.0 37.0 41.0 29.0 41.0 21 36.3298914091518 40.0 37.0 41.0 29.0 41.0 22 36.27904763703179 40.0 37.0 41.0 29.0 41.0 23 36.205751532557514 40.0 37.0 41.0 29.0 41.0 24 36.144715888185246 40.0 37.0 41.0 28.0 41.0 25 36.09488781272728 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42548333160283747 1101 2 0.566514441621905 1101 3 0.3897862739952771 1101 4 0.5424077386360437 1101 5 0.5123375621854009 1101 6 0.42981973854946176 1101 7 0.5229949257388355 1101 8 0.5101510900127337 1101 9 0.571472318766368 1101 10 0.6999741503411911 1101 11 0.7112966071162745 1101 12 0.7662948585748666 1101 13 0.7058456501117334 1101 14 0.8701449578719931 1101 15 0.8830853263409679 1101 16 0.7170886660308184 1101 17 0.8129069959717512 1101 18 0.7565495344639288 1101 19 0.7858794471455823 1101 20 0.6312723480999267 1101 21 0.6899764103794226 1101 22 0.7326931233806135 1101 23 0.8565645894425202 1101 24 0.8966870488473049 1101 25 0.8353148940759567 1102 1 0.370064991576065 1102 2 0.39219884590570686 1102 3 0.32395796074226624 1102 4 0.38980287473599873 1102 5 0.3132584680310089 1102 6 0.2489283953990764 1102 7 0.30670922738988793 1102 8 0.3832758111193826 1102 9 0.3740147105557412 1102 10 0.4440510133487763 1102 11 0.5097610203246674 1102 12 0.49886402412596453 1102 13 0.5208450923250325 1102 14 0.6049647927671273 1102 15 0.5919792353101769 1102 16 0.5540308793284581 1102 17 0.5949752690641219 1102 18 0.4794355228619622 1102 19 0.5050277068510738 1102 20 0.562940130340003 1102 21 0.4957059953861176 1102 22 0.6633172867005541 1102 23 0.6693479450873241 1102 24 0.6682934745501186 1102 25 0.6914856883642244 1103 1 0.36772615851151613 1103 2 0.39373628956447604 1103 3 0.431765925014691 1103 4 0.46015119104635716 1103 5 0.3963674013036922 1103 6 0.4024268585067716 1103 7 0.3849640620533563 1103 8 0.41752107983432296 1103 9 0.45773274939845976 1103 10 0.4674423764275275 1103 11 0.48750420079579726 1103 12 0.45915982420590495 1103 13 0.5142705013589293 1103 14 0.6821050703726073 1103 15 0.6567127257786893 1103 16 0.6746204119202233 1103 17 0.67188384120157 1103 18 0.6021153668294872 1103 19 0.7107594192109943 1103 20 0.5012574055608852 1103 21 0.5011511428037281 1103 22 0.5837456868163429 1103 23 0.6073086337253457 1103 24 0.7223413594279222 1103 25 0.5977188549137296 1104 1 0.13068149502633375 1104 2 0.13243519977538298 1104 3 0.13242207379544624 1104 4 0.24005561911295104 1104 5 0.26647100745775276 1104 6 0.1126739706507891 1104 7 0.1183915270193765 1104 8 0.17287982197292706 1104 9 0.13009944534631757 1104 10 0.2239628627213932 1104 11 0.19080216187528976 1104 12 0.06917792300066594 1104 13 0.14099770309869086 1104 14 0.16914464759405945 1104 15 0.18952272524860803 1104 16 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1107 15 0.4258928786832783 1107 16 0.41010452053723156 1107 17 0.3491802848907568 1107 18 0.4414137172725887 1107 19 0.3828598633318734 1107 20 0.4304227480928873 1107 21 0.32840301673179084 1107 22 0.4698784424508986 1107 23 0.41440570555049305 1107 24 0.3925837107322465 1107 25 0.38638247704479767 1108 1 0.3132313955209334 1108 2 0.3014127014491379 1108 3 0.2935323514297892 1108 4 0.4812691318261173 1108 5 0.29195361643514417 1108 6 0.12032028201104339 1108 7 0.08513868995540008 1108 8 0.2567169277543897 1108 9 0.2983349347450144 1108 10 0.36332859576932464 1108 11 0.3398922464944505 1108 12 0.3185627697977864 1108 13 0.35324933359795097 1108 14 0.2638191909994845 1108 15 0.3618814219639077 1108 16 0.32700623737110845 1108 17 0.34445022965584826 1108 18 0.30442373406334156 1108 19 0.33533650601036413 1108 20 0.3824362434046833 1108 21 0.4185611750474081 1108 22 0.2741026227823653 1108 23 0.1565819561081696 1108 24 0.19155769184125404 1108 25 0.21390286363334354 1109 1 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-0.3452615263522816 2214 11 -0.3699342051773513 2214 12 -0.35120533119365405 2214 13 -0.3583571270451955 2214 14 -0.48688304036527086 2214 15 -0.35073651158828056 2214 16 -0.5111741401817795 2214 17 -0.5208151871253577 2214 18 -0.6257473288732172 2214 19 -0.5483573512907256 2214 20 -0.49342850290647533 2214 21 -0.3665240050027876 2214 22 -0.49497151662849603 2214 23 -0.46207442232734053 2214 24 -0.4736409555047203 2214 25 -0.5259995007100429 2215 1 -0.5418192058656928 2215 2 -0.48927434868910424 2215 3 -0.43120623990731843 2215 4 -0.3212106649675448 2215 5 -0.4337440848647276 2215 6 -0.5452857537916103 2215 7 -0.5660171583245912 2215 8 -0.5584061595557301 2215 9 -0.6704700990463479 2215 10 -0.6675591821950135 2215 11 -0.6636812454705421 2215 12 -0.678976573599158 2215 13 -0.8086032950735529 2215 14 -0.8417939652691473 2215 15 -0.9118561229640108 2215 16 -0.9714586484526606 2215 17 -0.948549330959942 2215 18 -0.9735109999871199 2215 19 -0.7946663466394455 2215 20 -0.8015313133158557 2215 21 -0.7396105770506267 2215 22 -0.9218801951551683 2215 23 -0.8765623426814955 2215 24 -0.9772688976380195 2215 25 -1.0374498476770526 2216 1 -0.7122730288264734 2216 2 -0.6857112726495735 2216 3 -0.6683828167598023 2216 4 -0.5638043304841105 2216 5 -0.7476798648428087 2216 6 -0.7786848754399998 2216 7 -1.0354569338085327 2216 8 -1.0386302636899174 2216 9 -1.0812246975868902 2216 10 -1.0785222795470943 2216 11 -0.9589909326208357 2216 12 -1.0724989020756723 2216 13 -1.130163087070244 2216 14 -1.3459542404099736 2216 15 -1.2312768992362777 2216 16 -1.4106001288509376 2216 17 -1.7170677416885667 2216 18 -1.4193145920171801 2216 19 -1.456099946857229 2216 20 -1.5909409467668851 2216 21 -1.636794693888767 2216 22 -1.366271905469283 2216 23 -1.528695433278635 2216 24 -1.5133276999742833 2216 25 -1.7138180521008053 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35754.0 3 1.0 4 522.0 5 4649.0 6 5303.0 7 4248.0 8 3387.0 9 3021.0 10 2740.0 11 2611.0 12 2376.0 13 2299.0 14 2316.0 15 2252.0 16 2450.0 17 2572.0 18 2881.0 19 3333.0 20 4341.0 21 5731.0 22 7457.0 23 9104.0 24 10730.0 25 12191.0 26 13367.0 27 15222.0 28 17147.0 29 20801.0 30 26546.0 31 33982.0 32 43339.0 33 56796.0 34 75146.0 35 100156.0 36 142727.0 37 228531.0 38 841540.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.37103572263932 19.741699955512953 13.419207855403343 27.468056466444384 2 13.70774878607788 20.905326950644803 39.858020470003176 25.528903793274143 3 16.625007010038377 25.35796975554709 30.41967526068759 27.597347973726947 4 11.146907769559807 17.649933477346533 38.22137022360195 32.98178852949171 5 12.412831720548347 38.405874294406566 36.501459295506194 12.679834689538891 6 31.367004209912242 38.12152475534048 18.12007781332611 12.391393221421172 7 27.10958647703251 31.970168510480462 23.033715261420493 17.886529751066536 8 24.80367188796621 37.18467604859986 20.798933229555953 17.212718833877975 9 26.26401239276646 14.743592311403164 20.031462610824445 38.960932685005936 10 14.103756282550089 29.233035326486593 35.46600026666682 21.197208124296495 11 34.07350439381793 22.43636731940198 24.85298148456513 18.637146802214964 12 22.573586507886098 25.090454225269504 32.27300324050152 20.062956026342878 13 28.649512551435734 21.80211482350625 25.958402786957198 23.589969838100814 14 20.756406676310636 21.890335738221236 27.98194274047353 29.3713148449946 15 23.118457697521528 30.465978739609138 23.55180253254664 22.863761030322692 16 22.208908489450202 28.09411244992329 27.477541659299288 22.219437401327216 17 21.680559434795946 28.509803158439368 27.826306439132047 21.983330967632636 18 21.738956230170384 27.60649109280619 29.199213998744185 21.455338678279247 19 23.15021610019404 27.26782175696639 27.690466013073017 21.891496129766548 20 23.382195495571274 27.350965827386503 27.833350213925744 21.43348846311648 21 22.867457635265588 26.958471287427855 27.28246551481435 22.891605562492202 22 22.99741468991729 27.790252270872745 27.1764270991768 22.035905940033164 23 22.14768941297115 27.645326579218736 27.523665533986698 22.68331847382342 24 22.85471989947178 27.257521734478008 27.836783554755208 22.050974811295003 25 22.620992499858374 27.278439109916814 27.583949571032186 22.516618819192626 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 111.0 1 111.0 2 223.5 3 336.0 4 336.0 5 336.0 6 795.0 7 1254.0 8 1254.0 9 1254.0 10 1918.0 11 2582.0 12 2582.0 13 2582.0 14 4900.0 15 7218.0 16 7218.0 17 7218.0 18 14000.5 19 20783.0 20 20783.0 21 20783.0 22 33249.0 23 45715.0 24 45715.0 25 45715.0 26 64858.5 27 84002.0 28 84002.0 29 84002.0 30 107319.5 31 130637.0 32 130637.0 33 130637.0 34 149795.5 35 168954.0 36 168954.0 37 168954.0 38 183891.0 39 198828.0 40 198828.0 41 198828.0 42 208817.0 43 218806.0 44 218806.0 45 218806.0 46 226994.0 47 235182.0 48 235182.0 49 235182.0 50 224773.0 51 214364.0 52 214364.0 53 214364.0 54 190033.5 55 165703.0 56 165703.0 57 165703.0 58 140843.5 59 115984.0 60 115984.0 61 115984.0 62 93737.0 63 71490.0 64 71490.0 65 71490.0 66 54651.0 67 37812.0 68 37812.0 69 37812.0 70 27382.0 71 16952.0 72 16952.0 73 16952.0 74 11819.0 75 6686.0 76 6686.0 77 6686.0 78 4688.0 79 2690.0 80 2690.0 81 2690.0 82 1856.5 83 1023.0 84 1023.0 85 1023.0 86 692.0 87 361.0 88 361.0 89 361.0 90 226.0 91 91.0 92 91.0 93 91.0 94 48.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18545762713804148 2 0.0076677945191291445 3 0.004291676036826014 4 0.002517783274937928 5 0.08703519002683156 6 0.3413313007955623 7 0.03181562501967018 8 0.013676140970685563 9 0.005607790021452658 10 0.004005564301037613 11 0.0 12 0.0 13 0.0 14 9.727799016805631E-4 15 0.0 16 0.0 17 0.02157282487844543 18 0.02849672888452473 19 0.0 20 0.0 21 4.0055643010376125E-4 22 1.7166704147304055E-4 23 0.005207233591348896 24 0.0 25 1.1444469431536036E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1747569.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.60960298797487 #Duplication Level Percentage of deduplicated Percentage of total 1 82.11751645603907 58.8040275177545 2 12.134287341876824 17.378629981876166 3 3.1444170076527964 6.755113606399579 4 1.128048228533793 3.2311634318637297 5 0.5054976865579218 1.8099244322876267 6 0.26925887766563095 1.1568912798374116 7 0.15207640425331084 0.7623091652692853 8 0.10806425977147521 0.6190750991539778 9 0.07091033696966498 0.45700749703271026 >10 0.3360958480566585 4.187039468122591 >50 0.018869348910822044 0.9277331733276841 >100 0.012478916662697124 1.8347175081103129 >500 0.001519563030237494 0.7570108976812283 >1k 9.597240190973637E-4 1.3193569412832837 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2971 0.17000759340546784 No Hit CCCATGTACTCTGCGTTGATACCAC 2765 0.15821978989098573 No Hit GTATCAACGCAGAGTACTTTTTTTT 2539 0.14528753943335 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2045 0.11701969993745598 No Hit CTTTAGGACGTGAAATATGGCGAGG 1983 0.11347191441367979 No Hit GTCCTACAGTGGACATTTCTAAATT 1949 0.11152635461031868 No Hit TATCAACGCAGAGTACTTTTTTTTT 1930 0.11043913001432275 No Hit CTGTAGGACGTGGAATATGGCAAGA 1922 0.10998135123706132 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 5.722234715768018E-5 0.0 0.0 0.0 0.0 9 5.722234715768018E-5 0.0 0.0 5.722234715768018E-5 0.0 10 5.722234715768018E-5 0.0 0.0 5.722234715768018E-5 0.0 11 5.722234715768018E-5 0.0 0.0 5.722234715768018E-5 0.0 12 5.722234715768018E-5 0.0 0.0 5.722234715768018E-5 0.0 13 5.722234715768018E-5 0.0 0.0 5.722234715768018E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGCGAA 50 0.0015109762 13.286758 18 TAGGACC 510 0.0 12.880694 4 AGGACGT 1060 0.0 11.586285 5 TTTATAC 250 0.0 11.424943 3 GGACGTG 1045 0.0 11.205319 6 GTAGGAC 1295 0.0 11.174976 3 TGTAGGA 1340 0.0 11.155912 2 CCACGAC 60 0.005781319 11.107583 3 TATCCGT 60 0.005785027 11.106627 6 CCGCTCC 60 0.0059287134 11.070081 9 GTCCCGT 70 0.0014600545 10.884961 1 GACGTGG 500 0.0 10.819837 7 TCTATAC 195 1.8189894E-12 10.741399 3 GTCCTAT 160 5.2023097E-10 10.714884 1 ATAGGAC 240 0.0 10.710884 3 GGACCGT 80 3.69871E-4 10.709962 6 GGCGAGG 510 0.0 10.605862 19 TGTGCGC 90 9.634618E-5 10.542632 10 TAGGACA 425 0.0 10.528567 4 AGGACCT 880 0.0 10.494202 5 >>END_MODULE