FastQCFastQC Report
Thu 26 May 2016
SRR1513125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838027
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC50280.2735541969731674No Hit
CCCATGTACTCTGCGTTGATACCAC41240.22437102392946348No Hit
GAGTACATGGGAAGCAGTGGTATCA18960.103154088596087No Hit
GTATCAACGCAGAGTACTTTTTTTT18900.10282765160685887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGC250.006080613518.9738778
TTTAGAC1552.3646862E-1111.6812813
GTCCTAC3500.011.1900051
CGACTCT600.005924820411.07110615
CGCAGAT1053.524341E-610.8422158
TTAGACA1605.14774E-1010.720654
GTGTAAG3150.010.0073221
GTTAGAC1356.175287E-79.882413
TAGGACT3000.09.8471154
TTAGACT1553.8728103E-89.8368684
ATTACAC1752.4392648E-99.8017363
GTATAGG2053.6379788E-119.7854311
GTCTTAG2053.6379788E-119.7854311
TCTACAC2450.09.7239463
GAGTAAG2002.382876E-109.5524441
GTCTAAT1002.6071895E-49.5524441
ACCGTGC1002.7937844E-49.4869388
TAAGACT2650.09.3496654
AGGACGT2850.09.3433845
ATAAGAC1752.5376721E-89.2571953
Produced by FastQC (version 0.11.5)