##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513125_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1838027
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.032575691216724	31.0	30.0	34.0	22.0	34.0
2	28.882481595754577	31.0	30.0	34.0	16.0	34.0
3	29.07843791195668	31.0	30.0	34.0	20.0	34.0
4	32.12172617703657	35.0	33.0	37.0	19.0	37.0
5	32.249795568835495	35.0	33.0	37.0	19.0	37.0
6	32.603613004596774	37.0	33.0	37.0	25.0	37.0
7	32.54353554109923	37.0	33.0	37.0	23.0	37.0
8	32.41765273306649	37.0	33.0	37.0	22.0	37.0
9	33.60737029434279	39.0	34.0	39.0	18.0	39.0
10	33.175744970014044	38.0	33.0	39.0	17.0	39.0
11	33.62857999365624	38.0	34.0	39.0	21.0	39.0
12	33.61579998552796	38.0	34.0	39.0	19.0	39.0
13	33.64978806078474	39.0	34.0	39.0	19.0	39.0
14	33.82524195781672	39.0	33.0	41.0	10.0	41.0
15	34.23309396434329	39.0	33.0	41.0	17.0	41.0
16	34.077432486029856	39.0	33.0	41.0	16.0	41.0
17	33.66589500589491	39.0	33.0	41.0	10.0	41.0
18	33.38591544085043	39.0	32.0	41.0	10.0	41.0
19	34.02116943875144	39.0	33.0	41.0	16.0	41.0
20	34.23283553506015	39.0	34.0	41.0	16.0	41.0
21	34.28141643185873	39.0	34.0	41.0	13.0	41.0
22	33.958879276528585	39.0	34.0	41.0	8.0	41.0
23	33.48919085519418	39.0	33.0	41.0	2.0	41.0
24	33.72725373457517	39.0	34.0	41.0	2.0	41.0
25	33.363504453416624	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7725949356274775
1101	2	1.0138109581205264
1101	3	0.8767951134458158
1101	4	1.3387630240075907
1101	5	1.2042979324995002
1101	6	0.9296124557883516
1101	7	0.8953054820189834
1101	8	1.014227940682332
1101	9	1.269042831093401
1101	10	1.6196959846901535
1101	11	1.2574812654338672
1101	12	1.166771185360048
1101	13	0.9715910338105971
1101	14	1.9307765009937086
1101	15	1.5016622424121735
1101	16	1.5211310200199222
1101	17	2.0094672382770966
1101	18	2.04955612875343
1101	19	1.6354614203270614
1101	20	1.327582901951132
1101	21	1.2729614966796845
1101	22	1.642825938117923
1101	23	1.8382886859332785
1101	24	1.4747101078507114
1101	25	1.8116387637536846
1102	1	1.0449624861941516
1102	2	1.2373362550133216
1102	3	1.0659990611709915
1102	4	1.3572571403787208
1102	5	1.3949269656321945
1102	6	1.0938036601698968
1102	7	1.098399674307899
1102	8	1.1079929208894796
1102	9	1.4901967815299244
1102	10	1.6780621253549768
1102	11	1.187966597031938
1102	12	1.1625485293218745
1102	13	1.2008627652785222
1102	14	2.1957106151957575
1102	15	1.7868523080268375
1102	16	1.9102704376220032
1102	17	2.1724947313631517
1102	18	2.5011487755730215
1102	19	1.857584949419845
1102	20	1.5166268800434253
1102	21	1.4218503855685753
1102	22	1.6799437733763156
1102	23	1.8360225398457573
1102	24	1.6993150641979824
1102	25	2.084662460950149
1103	1	0.9465207529403941
1103	2	1.2045962301961204
1103	3	0.9178204202775717
1103	4	1.4221886784009925
1103	5	1.3427963576108297
1103	6	1.1843316911527495
1103	7	0.9699926765448836
1103	8	1.021245970442294
1103	9	1.3489249859755503
1103	10	1.6222250386708197
1103	11	1.3105696764255015
1103	12	1.2257084070392068
1103	13	1.309961295567959
1103	14	2.12256535729869
1103	15	1.6847164403050812
1103	16	1.6544659825484374
1103	17	2.0491821291210215
1103	18	2.18364052106363
1103	19	1.6764204732215973
1103	20	1.415526314571963
1103	21	1.4750028782371984
1103	22	1.6780551115729025
1103	23	1.9557585458224978
1103	24	1.7286207193419685
1103	25	2.0076754749516823
1104	1	0.8715089888205334
1104	2	1.059166537769567
1104	3	0.8713023347979956
1104	4	1.2229362829188375
1104	5	1.218782265043103
1104	6	1.0168596415357243
1104	7	0.8773389719599507
1104	8	0.9353871049061837
1104	9	1.2891334493589355
1104	10	1.444173892305038
1104	11	1.085586580723863
1104	12	1.0017692444961597
1104	13	1.075571143302092
1104	14	2.0153626954281094
1104	15	1.7253174914450895
1104	16	1.670498967656755
1104	17	2.1545951345144942
1104	18	2.2497653404949887
1104	19	1.5886641134430164
1104	20	1.3279823183534418
1104	21	1.2952692507655428
1104	22	1.554205557708599
1104	23	1.864447381672754
1104	24	1.6664180680696958
1104	25	1.9605856108537694
1105	1	0.8967454138306259
1105	2	1.1402833632249738
1105	3	1.0274532185150385
1105	4	1.3535751294561322
1105	5	1.2620104145499482
1105	6	1.041323691530316
1105	7	0.8528426680711618
1105	8	0.892127098069416
1105	9	1.2292950730939936
1105	10	1.6792185943517595
1105	11	1.1105230709555585
1105	12	1.0931812750400738
1105	13	1.018781420979039
1105	14	1.6517348038466722
1105	15	1.5000247014330057
1105	16	1.6300646134524186
1105	17	1.7687633449177511
1105	18	1.559335044465044
1105	19	1.4267540589788794
1105	20	1.1524722243386307
1105	21	1.1608774521377043
1105	22	1.3004910062584827
1105	23	1.6034622208434683
1105	24	1.4081764710683586
1105	25	1.64867498798084
1106	1	0.5177650090652683
1106	2	0.6258041180664442
1106	3	0.5857147560662455
1106	4	0.9918079085485374
1106	5	0.9012944064251087
1106	6	0.6242663437493263
1106	7	0.548672220267612
1106	8	0.4897066119090354
1106	9	0.7903172551237247
1106	10	0.7056266827468392
1106	11	0.6147208529096773
1106	12	0.5450996140034334
1106	13	0.42272308927336155
1106	14	1.3073334086529798
1106	15	1.0138433712862138
1106	16	1.174205862937285
1106	17	1.4753401405279476
1106	18	1.5006187232018533
1106	19	1.0531320902811956
1106	20	0.9097680076008032
1106	21	0.8404259045377955
1106	22	1.1448185678270875
1106	23	1.3684429848277375
1106	24	1.1076327728206223
1106	25	1.369314470956951
1107	1	0.67919965422508
1107	2	0.7895207248267369
1107	3	0.7457182714792943
1107	4	1.0577560383742721
1107	5	0.9076101771284684
1107	6	0.7170317255919656
1107	7	0.7027550668351168
1107	8	0.7649647272910016
1107	9	1.0062446236332718
1107	10	1.2546847813159658
1107	11	0.8166166285549963
1107	12	0.9496115702289032
1107	13	0.8597220473864553
1107	14	1.6612035472570525
1107	15	1.1846172971109965
1107	16	1.2418625344525083
1107	17	1.7485577879014542
1107	18	1.8193584221500316
1107	19	1.298946328722998
1107	20	1.0901662682355706
1107	21	0.9430247627927741
1107	22	1.3110716215237446
1107	23	1.4975967128213767
1107	24	1.2765593236145207
1107	25	1.434626769553077
1108	1	0.4054961261979493
1108	2	0.6512078061446012
1108	3	0.45009729659831876
1108	4	0.8513894066268506
1108	5	0.7347086925111128
1108	6	0.315553956804699
1108	7	0.29046822851458387
1108	8	0.4727274523108491
1108	9	0.6255157330759218
1108	10	0.7956623002573266
1108	11	0.4409843408542642
1108	12	0.25447557542366894
1108	13	0.33023388906785556
1108	14	1.2967422885917088
1108	15	0.8000714713132027
1108	16	0.8778182774840886
1108	17	1.0856595246475678
1108	18	1.325812563377653
1108	19	0.8087641314487328
1108	20	0.5707406855365704
1108	21	0.6522495302585227
1108	22	0.9280966543080993
1108	23	1.0860807188785557
1108	24	0.9106487050366354
1108	25	1.2130941107614177
1109	1	0.5260076807639962
1109	2	0.6965524314907547
1109	3	0.6406989819911537
1109	4	0.9744250724919681
1109	5	1.0017812193893576
1109	6	0.7341174729920681
1109	7	0.6512197643691522
1109	8	0.6179648743662796
1109	9	0.8712950126285222
1109	10	1.0422473668741787
1109	11	0.718639604981469
1109	12	0.550510866404565
1109	13	0.5446937196826269
1109	14	1.4728641025479305
1109	15	1.1138602616960256
1109	16	1.1412507264448237
1109	17	1.4779769914071252
1109	18	1.766787181777488
1109	19	1.1314680663231087
1109	20	0.9206742059007951
1109	21	0.9018044653990245
1109	22	1.1803035994599398
1109	23	1.4659816880134287
1109	24	1.2711415481217827
1109	25	1.5572895611423831
1110	1	0.24007796839847018
1110	2	0.3923857108837616
1110	3	0.38133651348459807
1110	4	0.772290268529602
1110	5	0.5590836606360128
1110	6	0.1446114862324137
1110	7	0.22546255008180793
1110	8	0.3054515202828725
1110	9	0.4660081209906224
1110	10	0.7038069018900757
1110	11	0.31743084852481473
1110	12	0.3490302507081182
1110	13	0.08484486355657594
1110	14	1.0050347906970245
1110	15	0.5954338150319103
1110	16	0.718472497623182
1110	17	1.1687226193125824
1110	18	0.7494572340472061
1110	19	0.46603345833365495
1110	20	0.3063922872526632
1110	21	0.10116975729632571
1110	22	0.46015772016174594
1110	23	-0.7090120703203553
1110	24	0.088599966295547
1110	25	0.35907137961562086
1111	1	0.6459189426286152
1111	2	1.0004933390181563
1111	3	0.92729886314239
1111	4	1.2737584885994693
1111	5	1.0584707634884722
1111	6	0.7828000172718816
1111	7	0.7996886026352854
1111	8	0.8978194728837252
1111	9	1.2126907045619006
1111	10	1.4302211977265813
1111	11	0.9818592757311606
1111	12	0.9128827436240741
1111	13	0.7861790782192699
1111	14	1.8379341415594226
1111	15	1.3513271332429824
1111	16	1.4173737857245712
1111	17	1.9063910817480263
1111	18	2.1195637942319507
1111	19	1.6768491274546804
1111	20	1.276370862039542
1111	21	0.9495323448648847
1111	22	1.329515090188238
1111	23	1.7930895751287892
1111	24	1.3180669486514986
1111	25	1.6611908442923138
1112	1	-0.057340652134197256
1112	2	0.20238100642970736
1112	3	0.07174519396111378
1112	4	0.4674264700623425
1112	5	0.1279146796814601
1112	6	0.025686529862426255
1112	7	-0.21107132957521912
1112	8	-0.0795433958716174
1112	9	0.16190917569887375
1112	10	0.3433030700363702
1112	11	-0.0773335493809455
1112	12	-0.06909676955138622
1112	13	-0.0667696747240214
1112	14	0.4014721531676173
1112	15	0.23960105078576532
1112	16	0.19830010214552374
1112	17	0.8480372865862762
1112	18	0.7525609596713068
1112	19	0.20288815140596483
1112	20	0.08938644462423895
1112	21	-0.10936700573576985
1112	22	0.18227199286832274
1112	23	0.5937186376241002
1112	24	0.39824956034668446
1112	25	0.6447434336409614
1113	1	-0.05064628092308965
1113	2	0.052672440363306094
1113	3	-0.004893440472955035
1113	4	0.3857844544822626
1113	5	0.2910129414447198
1113	6	-0.00808119881177305
1113	7	-0.04599633367132583
1113	8	-0.0228958840673954
1113	9	0.16250139757354987
1113	10	0.4033540106503395
1113	11	0.005777104952727541
1113	12	-0.045838536386362705
1113	13	-0.13496181914513272
1113	14	0.48125611730066
1113	15	0.1875188904270928
1113	16	0.33173853400873554
1113	17	0.6228371138052466
1113	18	0.4876502249050816
1113	19	0.38280944666789196
1113	20	0.01697272342728695
1113	21	0.13707820331438114
1113	22	0.1855513768145869
1113	23	0.37228092071688224
1113	24	0.21833958522119445
1113	25	0.5949256580477709
1114	1	-1.1112094354215465
1114	2	-0.8878181939361767
1114	3	-0.8733683924421562
1114	4	-0.502485452157746
1114	5	-3.7064774951167863
1114	6	-1.596432234983176
1114	7	-1.3089406439848652
1114	8	-0.9437697719947167
1114	9	-0.7411627766181468
1114	10	-0.5328669481103034
1114	11	-1.0712935086615687
1114	12	-1.0869875649070195
1114	13	-1.1658211493260495
1114	14	-6.652172260458617
1114	15	-2.8595907581282383
1114	16	-1.6414407101765107
1114	17	-0.7938008686489724
1114	18	-6.618384526526889
1114	19	-2.855802514119773
1114	20	-1.850822758544787
1114	21	-1.6260797128365851
1114	22	-1.4408792737108058
1114	23	-1.0727458878328804
1114	24	-1.056228951330624
1114	25	-0.959246002926804
1115	1	-0.7108643380524313
1115	2	-0.38140354905374707
1115	3	-0.5489452667786665
1115	4	-3.0949356324684807
1115	5	-1.2312650737254174
1115	6	-0.693036377340686
1115	7	-0.6159902134168824
1115	8	-0.4757199046321574
1115	9	-0.30714987686439343
1115	10	-0.6521414465113224
1115	11	-0.6150363397767933
1115	12	-0.695583466501418
1115	13	-0.8944711672613366
1115	14	-6.242065809194482
1115	15	-2.531664361284321
1115	16	-1.3030574578804277
1115	17	-0.4015746537122311
1115	18	-0.09579465356684835
1115	19	-0.5093973774713874
1115	20	-0.6121190712745275
1115	21	-0.7606609706935927
1115	22	-2.1617747300388785
1115	23	-1.150831654394068
1115	24	-0.814396773202958
1115	25	-0.32812353585936194
1116	1	-0.5375471805787946
1116	2	-0.166847757350034
1116	3	-0.29057253798105975
1116	4	-0.0825909641570064
1116	5	-0.7816405599719971
1116	6	-0.5540864497797671
1116	7	-0.4052949703766586
1116	8	-0.44264733064080986
1116	9	-0.36755894650159604
1116	10	-0.3699775136076511
1116	11	-0.673940582076817
1116	12	-0.8568125649541187
1116	13	-0.6919968291912042
1116	14	0.051473601548920556
1116	15	-0.46934833633437023
1116	16	-0.4602090307032256
1116	17	-0.08889042845811446
1116	18	-0.19696316660608204
1116	19	-0.31742180219219307
1116	20	-0.7022816078393461
1116	21	-0.774170589138393
1116	22	-0.8262516774371775
1116	23	-1.3894794955733332
1116	24	-1.196765433081957
1116	25	-0.4876344329470257
1201	1	0.6381287613209778
1201	2	0.8755393073386664
1201	3	0.8660341556815148
1201	4	1.2494959254943012
1201	5	1.1993013020034269
1201	6	0.8033708967395157
1201	7	0.6804199763870926
1201	8	0.7462976412778417
1201	9	0.833662352211249
1201	10	1.2840202272394237
1201	11	0.8117121245886025
1201	12	0.8068769465241061
1201	13	0.5957022411932513
1201	14	1.5516268537601405
1201	15	1.3102983497411884
1201	16	1.2569518724356499
1201	17	1.6961231775435053
1201	18	0.9818080576225157
1201	19	1.0142456482980649
1201	20	0.9530090410497536
1201	21	1.0008514995678297
1201	22	1.171130321223643
1201	23	1.5111017403542064
1201	24	1.2507342307482077
1201	25	1.6183795595067352
1202	1	1.0612685492629232
1202	2	1.1950771643485645
1202	3	1.2064384754555881
1202	4	1.6305639852742004
1202	5	1.4697210109760093
1202	6	1.1261329129958355
1202	7	1.0588314725678742
1202	8	1.2075265391717664
1202	9	1.3684592049372526
1202	10	1.769860369358355
1202	11	1.3313905509462103
1202	12	1.252451947911041
1202	13	1.2920300660816935
1202	14	2.1447532367261672
1202	15	1.9308823239986665
1202	16	1.8351029090299775
1202	17	2.301133299103462
1202	18	2.3225853302559187
1202	19	1.9666468478483665
1202	20	1.6750460466661892
1202	21	1.5537115269717034
1202	22	1.7728093784830392
1202	23	2.025121352842284
1202	24	1.8184562203754524
1202	25	2.2895324589756214
1203	1	0.5819891433085402
1203	2	0.620904603740069
1203	3	0.5804601838041101
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2211	12	-0.885984771848463
2211	13	-0.8204280447048262
2211	14	-0.15523323136638822
2211	15	-0.5956760343421621
2211	16	-7.093899683258954
2211	17	-2.3306741126671753
2211	18	-1.1533902274046
2211	19	-1.1236958808740383
2211	20	-1.2730551083219126
2211	21	-1.1752125271877674
2211	22	-1.0232992694671381
2211	23	-0.6889839360108709
2211	24	-1.0374024322180304
2211	25	-8.187759143016486
2212	1	-1.4675039722386494
2212	2	-3.876281037505912
2212	3	-1.8038061257302473
2212	4	-3.65212915762093
2212	5	-1.2914791917574355
2212	6	-1.1570023062420844
2212	7	-1.126042781752087
2212	8	-0.9756666162431067
2212	9	-1.1347834077684666
2212	10	-0.4351404465833326
2212	11	-0.9844255073042589
2212	12	-1.0869875649070195
2212	13	-1.3880810746738632
2212	14	-6.62909794759808
2212	15	-5.282732936614835
2212	16	-2.9393708085816677
2212	17	-7.544055364746697
2212	18	-3.161988191270698
2212	19	-2.0315744856162112
2212	20	-2.180310373068032
2212	21	-1.7319500894908089
2212	22	-1.503994305946975
2212	23	-0.9271741199333263
2212	24	-1.3307454087449457
2212	25	-1.0433993792417056
2213	1	-0.489812162622691
2213	2	-0.4254526293400538
2213	3	-0.42933980277257433
2213	4	-0.14900671568201318
2213	5	-0.4295442736465489
2213	6	-0.6579996137101922
2213	7	-0.5219343621657728
2213	8	-0.4926069075214663
2213	9	-0.23773080397739932
2213	10	-4.032029731008848
2213	11	-1.749453749714835
2213	12	-1.1265949186404498
2213	13	-1.1293766283423707
2213	14	-0.2931800933301645
2213	15	-0.8450501795256216
2213	16	-0.6209075628033034
2213	17	-0.31128215886629107
2213	18	-0.17708642364871707
2213	19	-0.8101120663482604
2213	20	-1.2036555819417742
2213	21	-1.1989342554998714
2213	22	-1.3491842022394636
2213	23	-1.596971933617823
2213	24	-1.1814656171230098
2213	25	-0.7611312850931569
2214	1	-1.0116513013655393
2214	2	-0.7190444030180956
2214	3	-0.6880694672127596
2214	4	-0.18100392022287082
2214	5	-3.5387101498648086
2214	6	-1.5215198088632214
2214	7	-1.2908043452067268
2214	8	-0.9609434274504096
2214	9	-0.8568922654425535
2214	10	-4.4183309891308085
2214	11	-1.8430648138246575
2214	12	-1.3122074532323253
2214	13	-1.0988545355620118
2214	14	-0.32694882089470667
2214	15	-0.6049342385190712
2214	16	-0.6675718888975766
2214	17	-0.28691073758585617
2214	18	0.20404312482840936
2214	19	-0.6613687277477212
2214	20	-1.01768347370151
2214	21	-1.1492126715459818
2214	22	-0.8164061761356507
2214	23	-0.5485126052886073
2214	24	-0.9532031504058907
2214	25	-0.6954557424395347
2215	1	-2.5050525822307357
2215	2	-5.023431973628078
2215	3	-3.1644174443307627
2215	4	-2.150768160337204
2215	5	-2.1765526587724864
2215	6	-2.3134123356156913
2215	7	-2.4768933294342865
2215	8	-2.6031152371794875
2215	9	-8.494529412138487
2215	10	-4.1985812228253
2215	11	-3.3771714748266533
2215	12	-3.7843681387290324
2215	13	-3.2220010185334793
2215	14	-8.357473093819909
2215	15	-5.161915403304857
2215	16	-9.624770294584795
2215	17	-5.0158307988199695
2215	18	-3.419647905102142
2215	19	-3.85258588143175
2215	20	-3.6241164797644565
2215	21	-3.7508400520327214
2215	22	-9.725667969079389
2215	23	-10.440708252658471
2215	24	-6.854888907696441
2215	25	-5.18557775584533
2216	1	-1.8329637701013368
2216	2	-1.7741855316864132
2216	3	-1.6513804862121653
2216	4	-6.791483139531401
2216	5	-3.491548789070187
2216	6	-2.6590716718386638
2216	7	-2.0935199533799675
2216	8	-7.74030740876189
2216	9	-3.914261250631096
2216	10	-2.1617616454795012
2216	11	-2.082862103451422
2216	12	-2.26789512128682
2216	13	-2.0813093757119248
2216	14	-1.2715928793222133
2216	15	-2.0467653711065736
2216	16	-1.754116718605701
2216	17	-1.4066215966088222
2216	18	-7.435135379279508
2216	19	-4.796018711080169
2216	20	-4.071973373644024
2216	21	-3.219704899972129
2216	22	-2.8196559483858366
2216	23	-2.0238120296060735
2216	24	-2.497044538654844
2216	25	-1.915984655534377
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	100503.0
3	5.0
4	819.0
5	4845.0
6	5933.0
7	5263.0
8	4819.0
9	4646.0
10	4239.0
11	4139.0
12	4052.0
13	4015.0
14	3922.0
15	4032.0
16	4018.0
17	4206.0
18	4992.0
19	5703.0
20	7378.0
21	9744.0
22	11991.0
23	14062.0
24	16213.0
25	18505.0
26	20731.0
27	23179.0
28	25744.0
29	30750.0
30	36937.0
31	44206.0
32	55128.0
33	71833.0
34	93587.0
35	124074.0
36	172225.0
37	246596.0
38	644993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.98251339495202	17.503881824667424	11.882825303447236	25.630779476933323
2	15.15948611393712	19.79091944059713	39.62389719660326	25.425697248862487
3	18.809452241544193	24.407925638089587	29.12782739954252	27.6547947208237
4	12.014018760560948	16.290713801131933	37.611212821775766	34.084054616531354
5	13.509846855781285	37.3657928816359	35.3142691310263	13.810091131556524
6	32.99876420739767	35.7281735137703	17.811194180510608	13.461868098321423
7	28.746516681023483	31.10507937423511	21.760506549809623	18.38789739493178
8	26.41987159081448	34.54706622720884	20.49143530648592	18.541626875490756
9	27.123775775509962	14.63763825751186	19.26909485474731	38.96949111223087
10	14.540177062807832	28.031189783925477	34.602234480611955	22.82639867265474
11	34.9999863957054	22.390981985193086	23.18558162440719	19.423449994694327
12	23.553142637591925	24.498331171902084	30.436017734801908	21.512508455704083
13	29.284612265888814	20.92030900091699	25.115439978014027	24.67963875518017
14	21.654117498525228	20.54896352360615	27.79107430892504	30.00584466894358
15	24.741108760990564	27.932549484068105	22.948328432914703	24.37801332202663
16	23.569110783277132	26.925787323055356	25.976259910069977	23.528841983597538
17	22.971460731154796	26.953590622547296	27.00121594245337	23.07373270384453
18	22.978379263859843	26.48024516598828	27.72517767840087	22.81619789175101
19	24.182787073489028	26.499380144825164	26.725336131298278	22.59249665038753
20	24.552364667217056	26.15923142941264	26.365654433849674	22.922749469520635
21	24.333160262501647	25.97450307445638	26.28978588199182	23.40255078105016
22	24.148481099401977	26.091528654481206	25.93536854927861	23.824621696838207
23	23.545466221423066	26.01273689847488	26.55717345779256	23.884623422309488
24	23.861763393100144	26.461347529311862	26.0982287686295	23.57866030895849
25	23.867855622814112	25.749147959511344	26.12403488930277	24.258961528371778
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	235.0
1	235.0
2	352.0
3	469.0
4	469.0
5	469.0
6	893.5
7	1318.0
8	1318.0
9	1318.0
10	1867.5
11	2417.0
12	2417.0
13	2417.0
14	4353.5
15	6290.0
16	6290.0
17	6290.0
18	11532.0
19	16774.0
20	16774.0
21	16774.0
22	26161.5
23	35549.0
24	35549.0
25	35549.0
26	50613.0
27	65677.0
28	65677.0
29	65677.0
30	83898.0
31	102119.0
32	102119.0
33	102119.0
34	121963.5
35	141808.0
36	141808.0
37	141808.0
38	161684.5
39	181561.0
40	181561.0
41	181561.0
42	198882.0
43	216203.0
44	216203.0
45	216203.0
46	234647.0
47	253091.0
48	253091.0
49	253091.0
50	250073.5
51	247056.0
52	247056.0
53	247056.0
54	226422.5
55	205789.0
56	205789.0
57	205789.0
58	179941.0
59	154093.0
60	154093.0
61	154093.0
62	127971.0
63	101849.0
64	101849.0
65	101849.0
66	80102.5
67	58356.0
68	58356.0
69	58356.0
70	43303.0
71	28250.0
72	28250.0
73	28250.0
74	20044.0
75	11838.0
76	11838.0
77	11838.0
78	8284.5
79	4731.0
80	4731.0
81	4731.0
82	3271.0
83	1811.0
84	1811.0
85	1811.0
86	1170.0
87	529.0
88	529.0
89	529.0
90	349.5
91	170.0
92	170.0
93	170.0
94	101.0
95	32.0
96	32.0
97	32.0
98	22.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17377329059910435
2	0.26430514894503726
3	0.2686576421347456
4	0.4768156289325456
5	0.2640331181206805
6	0.018498096056260325
7	0.018498096056260325
8	0.016757098780377
9	0.01811725290216085
10	0.02034790566188636
11	0.020239093332143653
12	0.02774714408439049
13	0.026767833116706122
14	0.024754805016466024
15	0.04183834078607115
16	0.020456717991629066
17	0.04183834078607115
18	0.031446763295642555
19	0.027257488600548305
20	0.029270516700788403
21	0.034221477704081606
22	0.04406899354579666
23	0.028889673546688924
24	0.04825826824089091
25	0.036615348958421175
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1838027.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.02775771789365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4781464607482	58.686881925778245
2	12.376646500140613	17.82924190944289
3	3.3339628493633358	7.2041360506320204
4	1.1999990447891915	3.4573296183911877
5	0.5688244741327425	2.048557570342072
6	0.3104680286262907	1.3417389567027922
7	0.17583531344516407	0.8865516348554715
8	0.12310170984133802	0.7093392104888271
9	0.07766899242179716	0.5034891031515112
>10	0.3327750901761219	3.990363118785054
>50	0.013765728502792755	0.6728233422387141
>100	0.007274826189420639	1.1550557956655783
>500	6.889417302978457E-4	0.3281178274625414
>1k	7.654908114420474E-4	0.9091471781263363
>5k	7.654908114420473E-5	0.277226757936759
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	5028	0.2735541969731674	No Hit
CCCATGTACTCTGCGTTGATACCAC	4124	0.22437102392946348	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1896	0.103154088596087	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1890	0.10282765160685887	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.44061648713539E-5
2	0.0	0.0	0.0	0.0	5.44061648713539E-5
3	0.0	0.0	0.0	0.0	5.44061648713539E-5
4	0.0	0.0	0.0	0.0	5.44061648713539E-5
5	0.0	0.0	0.0	0.0	5.44061648713539E-5
6	5.44061648713539E-5	0.0	0.0	0.0	5.44061648713539E-5
7	5.44061648713539E-5	0.0	0.0	0.0	5.44061648713539E-5
8	5.44061648713539E-5	0.0	0.0	5.44061648713539E-5	5.44061648713539E-5
9	5.44061648713539E-5	0.0	0.0	1.088123297427078E-4	5.44061648713539E-5
10	5.44061648713539E-5	0.0	0.0	1.088123297427078E-4	5.44061648713539E-5
11	1.088123297427078E-4	0.0	0.0	1.088123297427078E-4	5.44061648713539E-5
12	1.088123297427078E-4	0.0	0.0	1.088123297427078E-4	1.088123297427078E-4
13	1.088123297427078E-4	0.0	0.0	1.088123297427078E-4	1.088123297427078E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGCGC	25	0.0060806135	18.973877	8
TTTAGAC	155	2.3646862E-11	11.681281	3
GTCCTAC	350	0.0	11.190005	1
CGACTCT	60	0.0059248204	11.071106	15
CGCAGAT	105	3.524341E-6	10.842215	8
TTAGACA	160	5.14774E-10	10.72065	4
GTGTAAG	315	0.0	10.007322	1
GTTAGAC	135	6.175287E-7	9.88241	3
TAGGACT	300	0.0	9.847115	4
TTAGACT	155	3.8728103E-8	9.836868	4
ATTACAC	175	2.4392648E-9	9.801736	3
GTATAGG	205	3.6379788E-11	9.785431	1
GTCTTAG	205	3.6379788E-11	9.785431	1
TCTACAC	245	0.0	9.723946	3
GAGTAAG	200	2.382876E-10	9.552444	1
GTCTAAT	100	2.6071895E-4	9.552444	1
ACCGTGC	100	2.7937844E-4	9.486938	8
TAAGACT	265	0.0	9.349665	4
AGGACGT	285	0.0	9.343384	5
ATAAGAC	175	2.5376721E-8	9.257195	3
>>END_MODULE