FastQCFastQC Report
Thu 26 May 2016
SRR1513125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838027
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC57060.31044157675594536No Hit
CCCATGTACTCTGCGTTGATACCAC55580.302389464354985No Hit
GTATCAACGCAGAGTACTTTTTTTT29330.159573281567681No Hit
TATCAACGCAGAGTACTTTTTTTTT21140.11501463253804214No Hit
GAGTACATGGGAAGCAGTGGTATCA21050.11452497705419996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTACG600.00576285111.1124091
ACGGGCG600.005918111711.07279216
CTCGAAC1355.7678335E-810.54551618
GTCCTAA2800.010.2052741
CCTACGA750.002597491510.1568652
GCACCGT750.002603971410.1538166
CAATACT1605.9590093E-910.1152564
TCGAACT1252.6122689E-69.86738219
TAGGACG2950.09.6816024
GTCCTAC3550.09.6590761
TCTTATA2303.6379788E-129.5220612
GTAGTAC1401.0266576E-69.5212813
TAGAACC2106.366463E-119.5202414
TAGGACC2002.5465852E-109.5202414
ACCGAGC1106.922439E-59.4891578
CTAATAC2650.09.3416353
GTAGGAC3700.09.2639483
AGGACGT3100.09.2131375
TCCTACA4700.09.1168662
TCTACAC2304.0017767E-119.1073123
Produced by FastQC (version 0.11.5)