##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513125_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1838027 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.663746506444138 31.0 31.0 34.0 27.0 34.0 2 30.76768948443086 31.0 31.0 34.0 27.0 34.0 3 30.87948653637841 33.0 31.0 34.0 27.0 34.0 4 34.26858745818206 37.0 35.0 37.0 30.0 37.0 5 34.0026011587425 37.0 35.0 37.0 30.0 37.0 6 33.912937622787915 37.0 35.0 37.0 30.0 37.0 7 33.777811207343525 37.0 35.0 37.0 29.0 37.0 8 33.74438460370821 37.0 35.0 37.0 28.0 37.0 9 35.291917365740545 39.0 35.0 39.0 29.0 39.0 10 35.13951100827137 39.0 35.0 39.0 28.0 39.0 11 35.154931347580856 39.0 35.0 39.0 28.0 39.0 12 35.083434574138465 39.0 35.0 39.0 28.0 39.0 13 35.04771039816064 39.0 35.0 39.0 27.0 39.0 14 36.207280415358426 40.0 36.0 41.0 27.0 41.0 15 36.1745572834349 40.0 36.0 41.0 27.0 41.0 16 36.12758789723981 40.0 36.0 41.0 27.0 41.0 17 36.06255675243073 40.0 36.0 41.0 27.0 41.0 18 36.0177614365839 40.0 36.0 41.0 27.0 41.0 19 36.08978268545565 40.0 37.0 41.0 27.0 41.0 20 36.05566893195802 40.0 36.0 41.0 27.0 41.0 21 36.000857441158374 40.0 36.0 41.0 27.0 41.0 22 35.93890296497277 40.0 36.0 41.0 27.0 41.0 23 35.85920935873086 40.0 36.0 41.0 27.0 41.0 24 35.78764838601391 40.0 36.0 41.0 27.0 41.0 25 35.730555644721214 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4288976727506544 1101 2 0.4470341563275966 1101 3 0.45583637545353994 1101 4 0.6589667346517274 1101 5 0.4301279357788985 1101 6 0.590316375880235 1101 7 0.6017854666731779 1101 8 0.5131472167156232 1101 9 0.6557601180691037 1101 10 0.6836237191002823 1101 11 0.6202391226005517 1101 12 0.6585966763547475 1101 13 0.5625036351982899 1101 14 0.6873731230453402 1101 15 0.6461870545934261 1101 16 0.70659300606183 1101 17 0.5809520213133723 1101 18 0.5908513473090622 1101 19 0.536960415528803 1101 20 0.567569177119168 1101 21 0.6918566085601725 1101 22 0.6582382951088945 1101 23 0.5897556705392333 1101 24 0.675703832688086 1101 25 0.5924298245714823 1102 1 0.49256612890813045 1102 2 0.4781496055982437 1102 3 0.42069782548336576 1102 4 0.4857868637119722 1102 5 0.4931125895395425 1102 6 0.5308196023863943 1102 7 0.5982937905167844 1102 8 0.5555809242152137 1102 9 0.5461353688689528 1102 10 0.6680844316429813 1102 11 0.6312737736498448 1102 12 0.7253750506294594 1102 13 0.6237676664330323 1102 14 0.7683351099224112 1102 15 0.6765015711597755 1102 16 0.6861564787585408 1102 17 0.8475574114765934 1102 18 0.7407515696397411 1102 19 0.7628565166079184 1102 20 0.7242461469147301 1102 21 0.7379170174317125 1102 22 0.695595813681642 1102 23 0.7582243002230911 1102 24 0.950700850203404 1102 25 0.8903269017364934 1103 1 0.4960987154355685 1103 2 0.4620200366360585 1103 3 0.4852998271750586 1103 4 0.6339075405602728 1103 5 0.6401040409162988 1103 6 0.5355227406027296 1103 7 0.6542151397480112 1103 8 0.7381977217661273 1103 9 0.7284569791530586 1103 10 0.6047576389438802 1103 11 0.6232645380453263 1103 12 0.589089236363435 1103 13 0.7226307124221378 1103 14 0.7606097907981422 1103 15 0.6033321615772422 1103 16 0.6851565989802637 1103 17 0.6590111067918158 1103 18 0.6175967584093129 1103 19 0.6475284615807197 1103 20 0.6245839484887767 1103 21 0.5245572710995461 1103 22 0.5282632761016188 1103 23 0.586126366748644 1103 24 0.657423176665489 1103 25 0.6291317904669924 1104 1 0.3228614522149762 1104 2 0.31742058901977543 1104 3 0.3564405864589091 1104 4 0.5501560157289731 1104 5 0.3661028517863443 1104 6 0.3902291039317447 1104 7 0.4650835967512137 1104 8 0.47089969633655215 1104 9 0.387654401221063 1104 10 0.6151789534093979 1104 11 0.519756048272157 1104 12 0.5113860323894954 1104 13 0.5252454735310153 1104 14 0.6388278339904758 1104 15 0.597464276882576 1104 16 0.6367770534992161 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-1.1482002666295017 2215 23 -1.0369494137995616 2215 24 -1.1440554565849794 2215 25 -1.3187725285109408 2216 1 -0.26021802861292187 2216 2 -0.273914495848274 2216 3 -0.25694387746036895 2216 4 -0.1521988294542993 2216 5 -0.29333185450956734 2216 6 -0.38935735617502587 2216 7 -0.5434078189575331 2216 8 -0.34721595325872556 2216 9 -0.43875813043456446 2216 10 -0.3043430690730702 2216 11 -0.4267799134464312 2216 12 -0.4388782347653617 2216 13 -0.5083023591313989 2216 14 -0.6525476725984163 2216 15 -0.5744519805535049 2216 16 -0.5827153058953272 2216 17 -0.6638020401600073 2216 18 -0.6185177179895263 2216 19 -0.662085531998315 2216 20 -0.5895488643731142 2216 21 -0.8157420593665634 2216 22 -0.5803328763055333 2216 23 -0.846076200213659 2216 24 -0.7593206936608681 2216 25 -0.7656157179333576 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46939.0 3 1.0 4 767.0 5 6232.0 6 7101.0 7 5484.0 8 4218.0 9 3850.0 10 3460.0 11 3076.0 12 2952.0 13 2726.0 14 2652.0 15 2735.0 16 2775.0 17 3013.0 18 3290.0 19 3858.0 20 4986.0 21 6616.0 22 8094.0 23 9902.0 24 11226.0 25 12873.0 26 14102.0 27 15601.0 28 17878.0 29 21268.0 30 26677.0 31 34083.0 32 43894.0 33 57160.0 34 75705.0 35 101673.0 36 145109.0 37 236138.0 38 889913.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.744883159269996 18.388195851367843 13.003269785362765 27.863651203999396 2 14.454077618450134 20.142752829102513 37.9045869602832 27.498582592164155 3 17.156714452673572 24.28043501444027 29.53366747990659 29.02918305297957 4 11.820595031412083 16.778519108132976 36.089537085953275 35.311348774501674 5 13.477035526980643 37.41685614970467 35.22702028121896 13.879088042095725 6 33.11475141378632 36.09429245180026 17.39311993711653 13.397836197296883 7 28.40271432388704 31.05510660888561 21.940113555782794 18.602065511444557 8 26.464413859000548 34.92044144902017 20.03905829142557 18.576086400553717 9 26.762843183128936 14.461299063081492 18.938866339488776 39.83699141430079 10 14.960118827395592 28.284619904676923 33.94785523079936 22.807406037128118 11 35.03490427507322 22.101035512535997 23.257928202360468 19.60613201003032 12 23.275174956624685 24.489520556553305 30.564295301429194 21.671009185392816 13 29.149462983949636 20.779455361645937 25.288910337008108 24.78217131739632 14 22.128297411037572 20.57149230547417 26.70894099276657 30.591269290721684 15 24.69272758234781 28.001601717493813 22.852928711058105 24.452741989100268 16 23.75879135616615 26.943075373756752 25.56551127921407 23.732621990863027 17 23.230784718651115 27.146677985378183 26.373849423045005 23.248687872925696 18 23.377384582480037 26.553471845423164 27.047898726571866 23.021244845524933 19 24.056284265682713 26.572406172488215 26.701838438717168 22.669471123111904 20 24.398879885877626 26.0108257386861 26.47735860245796 23.112935772978307 21 24.213268974117213 25.888824749716676 26.415370478075012 23.482535798091096 22 24.108472894289854 26.085757219974038 25.82542357942706 23.98034630630905 23 23.619446309473418 25.993431712553633 26.18179661596085 24.205325362012097 24 23.74421050398063 26.506737931488495 26.139441912441985 23.6096096520889 25 23.79426155167808 25.755390438530966 26.07350397682728 24.376844032963678 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 214.0 1 214.0 2 305.5 3 397.0 4 397.0 5 397.0 6 887.0 7 1377.0 8 1377.0 9 1377.0 10 1850.5 11 2324.0 12 2324.0 13 2324.0 14 4079.5 15 5835.0 16 5835.0 17 5835.0 18 11084.5 19 16334.0 20 16334.0 21 16334.0 22 25684.5 23 35035.0 24 35035.0 25 35035.0 26 49832.5 27 64630.0 28 64630.0 29 64630.0 30 82309.5 31 99989.0 32 99989.0 33 99989.0 34 119429.5 35 138870.0 36 138870.0 37 138870.0 38 158215.0 39 177560.0 40 177560.0 41 177560.0 42 195211.0 43 212862.0 44 212862.0 45 212862.0 46 233780.0 47 254698.0 48 254698.0 49 254698.0 50 252432.5 51 250167.0 52 250167.0 53 250167.0 54 229059.5 55 207952.0 56 207952.0 57 207952.0 58 182441.0 59 156930.0 60 156930.0 61 156930.0 62 131086.5 63 105243.0 64 105243.0 65 105243.0 66 82353.0 67 59463.0 68 59463.0 69 59463.0 70 44080.0 71 28697.0 72 28697.0 73 28697.0 74 20355.5 75 12014.0 76 12014.0 77 12014.0 78 8450.5 79 4887.0 80 4887.0 81 4887.0 82 3362.0 83 1837.0 84 1837.0 85 1837.0 86 1198.0 87 559.0 88 559.0 89 559.0 90 350.0 91 141.0 92 141.0 93 141.0 94 76.0 95 11.0 96 11.0 97 11.0 98 6.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18226065231903557 2 0.008977017203773394 3 0.006039084300720283 4 0.002176246594854156 5 0.0789433452283345 6 0.33008220227450413 7 0.028073581073618615 8 0.014200009031423369 9 0.004733336343807789 10 0.0036452130463807113 11 0.0 12 0.0 13 0.0 14 0.0017409972758833246 15 0.0 16 0.0 17 0.019695031683430113 18 0.029705766019759234 19 0.0 20 0.0 21 5.44061648713539E-4 22 5.4406164871353895E-5 23 0.00571264731149216 24 0.0 25 2.1762465948541558E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1838027.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.41309857689077 #Duplication Level Percentage of deduplicated Percentage of total 1 79.25938984639284 54.22380450705489 2 13.425566102245698 18.369691544069966 3 3.8720139310140524 7.946894122606761 4 1.4524649753276333 3.9747051814628245 5 0.7118799157012649 2.4350955423889666 6 0.36210196437539616 1.4863510430219862 7 0.22190272030920788 1.0626736875295846 8 0.14598341375632487 0.7989742140721989 9 0.10332416986516142 0.6361853956524627 >10 0.41621755143048267 4.73812976241 >50 0.017766907266512828 0.8560898730800259 >100 0.009159373403827706 1.3140519093963718 >500 0.0013533996962712277 0.649765813729885 >1k 7.165057215553548E-4 0.8940951884484309 >5k 1.5922349367896774E-4 0.6134922150757265 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 5706 0.31044157675594536 No Hit CCCATGTACTCTGCGTTGATACCAC 5558 0.302389464354985 No Hit GTATCAACGCAGAGTACTTTTTTTT 2933 0.159573281567681 No Hit TATCAACGCAGAGTACTTTTTTTTT 2114 0.11501463253804214 No Hit GAGTACATGGGAAGCAGTGGTATCA 2105 0.11452497705419996 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.44061648713539E-5 2 0.0 0.0 0.0 0.0 5.44061648713539E-5 3 0.0 0.0 0.0 0.0 5.44061648713539E-5 4 0.0 0.0 0.0 0.0 5.44061648713539E-5 5 0.0 0.0 0.0 0.0 5.44061648713539E-5 6 0.0 0.0 0.0 0.0 5.44061648713539E-5 7 0.0 0.0 0.0 0.0 5.44061648713539E-5 8 5.44061648713539E-5 0.0 0.0 0.0 5.44061648713539E-5 9 5.44061648713539E-5 0.0 0.0 5.44061648713539E-5 5.44061648713539E-5 10 5.44061648713539E-5 0.0 0.0 5.44061648713539E-5 1.088123297427078E-4 11 5.44061648713539E-5 0.0 0.0 5.44061648713539E-5 1.632184946140617E-4 12 5.44061648713539E-5 0.0 0.0 5.44061648713539E-5 2.176246594854156E-4 13 5.44061648713539E-5 0.0 0.0 5.44061648713539E-5 3.8084315409947733E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCTACG 60 0.005762851 11.112409 1 ACGGGCG 60 0.0059181117 11.072792 16 CTCGAAC 135 5.7678335E-8 10.545516 18 GTCCTAA 280 0.0 10.205274 1 CCTACGA 75 0.0025974915 10.156865 2 GCACCGT 75 0.0026039714 10.153816 6 CAATACT 160 5.9590093E-9 10.115256 4 TCGAACT 125 2.6122689E-6 9.867382 19 TAGGACG 295 0.0 9.681602 4 GTCCTAC 355 0.0 9.659076 1 TCTTATA 230 3.6379788E-12 9.522061 2 GTAGTAC 140 1.0266576E-6 9.521281 3 TAGAACC 210 6.366463E-11 9.520241 4 TAGGACC 200 2.5465852E-10 9.520241 4 ACCGAGC 110 6.922439E-5 9.489157 8 CTAATAC 265 0.0 9.341635 3 GTAGGAC 370 0.0 9.263948 3 AGGACGT 310 0.0 9.213137 5 TCCTACA 470 0.0 9.116866 2 TCTACAC 230 4.0017767E-11 9.107312 3 >>END_MODULE