FastQCFastQC Report
Thu 26 May 2016
SRR1513124_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513124_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197918
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC29590.13462740648195248No Hit
GTCCTAAAGTGTGTATTTCTCATTT26330.11979518799154472No Hit
GTCCTACAGTGGACATTTCTAAATT26020.11838476230687404No Hit
GTATCAACGCAGAGTACTTTTTTTT25830.1175203078549791No Hit
CCCATGTACTCTGCGTTGATACCAC24200.11010419860977526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5700.012.3731664
ACCGTCC701.1057122E-412.1971258
TATACGG550.003043944712.1042225
ATTGCGA550.003101624512.0739218
AGGACCT11250.010.9898655
ACCGAGG951.3821616E-510.9845468
GGCGAGG6700.010.90579819
GTCCTAC13650.010.7799221
CTACACT3600.010.5896464
TGGCGAG14800.010.57922518
GTATAAT3400.010.3980131
TCCTACA14750.010.2815092
TAGGTTG1502.4045221E-810.144495
GTCTAGA2450.010.1399261
GTCTTAT2450.010.1399261
GGACCTG11250.010.1184056
CGCAGAT1701.5988917E-910.0446918
AGACCGT856.6852383E-410.0440056
TGTAGGA14950.09.888772
GTATAAG3300.09.8444741
Produced by FastQC (version 0.11.5)