##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513124_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2197918
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.555189957041165	31.0	30.0	34.0	25.0	34.0
2	29.42286700413755	31.0	30.0	34.0	23.0	34.0
3	29.61384774136251	31.0	30.0	34.0	25.0	34.0
4	32.6945345549743	37.0	33.0	37.0	24.0	37.0
5	32.83572863045846	37.0	33.0	37.0	25.0	37.0
6	33.19929132933986	37.0	35.0	37.0	27.0	37.0
7	33.158966804039096	37.0	35.0	37.0	27.0	37.0
8	33.0436217365707	37.0	35.0	37.0	26.0	37.0
9	34.27750989800347	39.0	35.0	39.0	25.0	39.0
10	33.8515408673117	39.0	34.0	39.0	22.0	39.0
11	34.306846752244624	39.0	35.0	39.0	27.0	39.0
12	34.30502093344702	39.0	35.0	39.0	26.0	39.0
13	34.3413762478855	39.0	35.0	39.0	27.0	39.0
14	34.55700167158192	39.0	34.0	41.0	17.0	41.0
15	34.99362715078543	40.0	34.0	41.0	24.0	41.0
16	34.836299170396714	39.0	34.0	41.0	21.0	41.0
17	34.44494835567114	39.0	34.0	41.0	17.0	41.0
18	34.18280800284633	39.0	33.0	41.0	17.0	41.0
19	34.82383737700861	39.0	34.0	41.0	22.0	41.0
20	35.04105385187255	40.0	35.0	41.0	24.0	41.0
21	35.08638311347375	40.0	35.0	41.0	25.0	41.0
22	34.77294421356939	40.0	35.0	41.0	18.0	41.0
23	34.30361869733084	39.0	34.0	41.0	16.0	41.0
24	34.55043227272355	39.0	34.0	41.0	18.0	41.0
25	34.185214826030816	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.949462798764177
1101	2	1.2592655221147169
1101	3	1.1385230672002429
1101	4	1.5647357994615305
1101	5	1.3798598832794156
1101	6	1.119005089642208
1101	7	1.050485447369276
1101	8	1.2407989908100276
1101	9	1.4872808546376888
1101	10	1.9745597775759336
1101	11	1.4329210486299573
1101	12	1.3100613143466902
1101	13	1.1557316904819572
1101	14	2.188319174852218
1101	15	1.9143826346945971
1101	16	2.0244109828656818
1101	17	2.350532796858978
1101	18	2.3844505601505404
1101	19	1.857325446100333
1101	20	1.4297370301406787
1101	21	1.4697127291182284
1101	22	1.7790923326041437
1101	23	2.0412452714445877
1101	24	1.671374949900823
1101	25	1.8567527226276752
1102	1	0.8541602671147821
1102	2	1.0993170798784497
1102	3	1.0020173901041574
1102	4	1.3390651858757892
1102	5	1.2473177470472407
1102	6	0.9256868972465213
1102	7	0.933281065865998
1102	8	0.9794950417754507
1102	9	1.3209545568538275
1102	10	1.5195666680625308
1102	11	1.1536954123932546
1102	12	1.0728223141925568
1102	13	1.0450290723992666
1102	14	2.0715072942026964
1102	15	1.6175488586172762
1102	16	1.8087141975194498
1102	17	2.0735643238572976
1102	18	2.2766212447566474
1102	19	1.7831571399209594
1102	20	1.4807560968423985
1102	21	1.3172735573063648
1102	22	1.628812482139793
1102	23	1.8738068249470956
1102	24	1.6925696182357157
1102	25	1.8894084914458062
1103	1	0.5130177990000675
1103	2	0.8100738392290374
1103	3	0.5718445810612245
1103	4	1.101463998525908
1103	5	0.893398109100481
1103	6	0.6519804814258876
1103	7	0.5410916814607276
1103	8	0.5983889829245541
1103	9	0.9367115167702735
1103	10	1.1458444319069159
1103	11	0.7979046074568572
1103	12	0.714323026669966
1103	13	0.6502549189277715
1103	14	1.5310352596438932
1103	15	1.1417207934180027
1103	16	1.1989879905347323
1103	17	1.527730057986446
1103	18	1.7957217687396394
1103	19	1.2869111891956777
1103	20	0.8939361978601141
1103	21	0.9311044289280943
1103	22	1.1648551977940116
1103	23	1.500765669207894
1103	24	1.2928983775428122
1103	25	1.502522761875312
1104	1	0.8303002202992715
1104	2	0.9623294286669939
1104	3	0.7617792892936741
1104	4	1.1704777162499553
1104	5	1.0849706116459572
1104	6	0.859483184771463
1104	7	0.727409010920745
1104	8	0.6877805918613973
1104	9	1.0159067698915152
1104	10	1.2683582952116623
1104	11	0.8383346168342243
1104	12	0.8224871349120448
1104	13	0.763574507627105
1104	14	1.8847834585258028
1104	15	1.442069828079461
1104	16	1.5472535551268578
1104	17	1.818562174772083
1104	18	1.9422390946832593
1104	19	1.310492407988228
1104	20	1.1025496041160423
1104	21	0.9815875607605022
1104	22	1.375441928953741
1104	23	1.5889680546324811
1104	24	1.4355792402679057
1104	25	1.767745563853076
1105	1	0.7110534407896019
1105	2	0.846832185768335
1105	3	0.7613752444002451
1105	4	1.1204569584435617
1105	5	1.1235427573970753
1105	6	0.8195322358215265
1105	7	0.7737984152474624
1105	8	0.829967020195447
1105	9	1.0939601624039526
1105	10	1.45137952070003
1105	11	0.9226123209299288
1105	12	0.8310054113777454
1105	13	0.7743210916800578
1105	14	1.694915692318304
1105	15	1.2982389370291614
1105	16	1.4256511938884984
1105	17	1.7858587810980069
1105	18	1.5406386728603962
1105	19	1.2610534748283655
1105	20	1.124540757553099
1105	21	1.1338326867164454
1105	22	1.1038439617036033
1105	23	1.492964967729499
1105	24	1.202955443437844
1105	25	1.5078255041352406
1106	1	0.5806459172786269
1106	2	0.8975739477544771
1106	3	0.7604421445166203
1106	4	1.2192774782535807
1106	5	1.1459119923600127
1106	6	0.7105335882921437
1106	7	0.7794766949887091
1106	8	0.8316591811080016
1106	9	1.0188068299792548
1106	10	0.9972261777910632
1106	11	0.9212493660437318
1106	12	0.7789216630421194
1106	13	0.6395688873626355
1106	14	1.7065280679486676
1106	15	1.26880330519473
1106	16	1.3673817272232327
1106	17	1.6911187385318769
1106	18	1.8113781283074957
1106	19	1.2308210385989256
1106	20	1.0591818286259524
1106	21	0.9330442697361292
1106	22	1.1430425442305179
1106	23	1.538267491292892
1106	24	1.1015788590027853
1106	25	1.4846741427529935
1107	1	0.5541478122269012
1107	2	0.7573550518099985
1107	3	0.7528991814265673
1107	4	1.0126258909256194
1107	5	0.892758042073325
1107	6	0.7165936499329817
1107	7	0.6821652847860804
1107	8	0.738878399539523
1107	9	0.990369221341993
1107	10	1.2702669791399401
1107	11	0.8761074672507689
1107	12	0.7935170316250577
1107	13	0.7465347872766657
1107	14	1.651435063222749
1107	15	1.0923995657822303
1107	16	1.2143875831927886
1107	17	1.6655059008524589
1107	18	1.7484556949765278
1107	19	1.233150580837993
1107	20	1.0228355619749792
1107	21	0.9012615092290233
1107	22	1.0244657631183784
1107	23	1.368522525549281
1107	24	1.1162629788961027
1107	25	1.3759170473641973
1108	1	0.3984186809261665
1108	2	0.6712480619598509
1108	3	0.47920636780720827
1108	4	0.8169469934666154
1108	5	0.6847669629956599
1108	6	0.31752345800790494
1108	7	0.29331408046508045
1108	8	0.22656428287302788
1108	9	0.6762098078796228
1108	10	0.8811686164366819
1108	11	0.5160291928068048
1108	12	0.4422680286144711
1108	13	0.2993559638772254
1108	14	1.3394374500162982
1108	15	0.797641293716616
1108	16	0.870483494014529
1108	17	1.2643041657830238
1108	18	1.469678103393349
1108	19	0.8522828561340816
1108	20	0.6843538286506075
1108	21	0.5980030997750134
1108	22	0.7879378299992581
1108	23	1.0409864782468574
1108	24	0.7742258590494444
1108	25	1.2074952336755302
1109	1	0.3214808284198618
1109	2	0.4905106943012747
1109	3	0.44371906733407585
1109	4	0.7852406778260885
1109	5	0.79114594731859
1109	6	0.4619471355149827
1109	7	0.3571857978085262
1109	8	0.3515726701893911
1109	9	0.6096136468195041
1109	10	0.9326552660888296
1109	11	0.591527903506794
1109	12	0.4745928327858451
1109	13	0.4349353249253838
1109	14	1.3575326722184684
1109	15	0.8315688140921438
1109	16	1.103925888535116
1109	17	1.4474525609634696
1109	18	1.551608190896097
1109	19	0.8402909927870184
1109	20	0.6729646352688121
1109	21	0.5652816146515889
1109	22	0.8732774267892367
1109	23	1.3593476261810409
1109	24	1.1269065148906492
1109	25	1.2312361193135573
1110	1	0.4632175158131844
1110	2	0.7236301729361756
1110	3	0.5729094101517376
1110	4	0.9988538686820903
1110	5	0.7497937996262181
1110	6	0.5011903828816742
1110	7	0.5416007574084531
1110	8	0.520953479785895
1110	9	0.6844994595063696
1110	10	1.014192072588905
1110	11	0.6473896633914009
1110	12	0.5764372537094502
1110	13	0.40425155335028506
1110	14	1.3235084503782417
1110	15	0.9138150599322898
1110	16	1.0216954195717847
1110	17	1.4099423428351443
1110	18	1.1323340485904012
1110	19	0.8138450463607825
1110	20	0.6906208675252472
1110	21	0.5264782994269908
1110	22	0.6578552696394127
1110	23	-0.530126423023745
1110	24	0.12239363463107367
1110	25	0.644215894571083
1111	1	0.2699451156190982
1111	2	0.6611041385499661
1111	3	0.6607319879872229
1111	4	0.9625418588317203
1111	5	0.7628499540234017
1111	6	0.5385817522542737
1111	7	0.540201294963822
1111	8	0.5246414189501891
1111	9	0.7387095309793068
1111	10	1.121769767864862
1111	11	0.5630673137152797
1111	12	0.4999360542794662
1111	13	0.31859687191939656
1111	14	1.2934936391386955
1111	15	0.9072478090020226
1111	16	0.9641040857189651
1111	17	1.3578804534376303
1111	18	1.649364764704906
1111	19	0.9814630710359893
1111	20	0.7034122380875019
1111	21	0.5226085125711819
1111	22	0.9323797676675554
1111	23	1.119369714412322
1111	24	0.8373817932872427
1111	25	1.0589185353564616
1112	1	-0.061387045175195
1112	2	0.07426538553470863
1112	3	0.10110841173409568
1112	4	0.5166590975668086
1112	5	0.2106365347114192
1112	6	-0.004410402556203508
1112	7	-0.1766882593977641
1112	8	0.06841687013943698
1112	9	0.15848371476682388
1112	10	0.32090924556086264
1112	11	-0.07784792556792297
1112	12	-0.013578312833018913
1112	13	-0.1241983805540201
1112	14	0.413399792998959
1112	15	0.2632092327166333
1112	16	0.25555808959792614
1112	17	0.7248480997143645
1112	18	0.6928963802991106
1112	19	0.3030278842691274
1112	20	0.06826330310229167
1112	21	-0.03667885582806463
1112	22	0.2464001106199376
1112	23	0.6280352171432497
1112	24	0.38978776800644965
1112	25	0.8210708361558545
1113	1	0.1291427031680783
1113	2	0.30039961220290223
1113	3	0.23312372934883996
1113	4	0.48105155925492227
1113	5	0.5076218695538941
1113	6	0.2439877792675489
1113	7	0.20022750494108266
1113	8	0.22812047980205818
1113	9	0.4101542126505038
1113	10	0.6136207986096451
1113	11	0.24565848629476505
1113	12	0.07409592367473294
1113	13	-0.004573800154730634
1113	14	0.5748848250727221
1113	15	0.33045176626343675
1113	16	0.45664122298377663
1113	17	0.6787194762223052
1113	18	0.684925114256707
1113	19	0.5103129312348287
1113	20	0.2621015773512454
1113	21	0.28335209084009705
1113	22	0.42828420579369464
1113	23	0.41623277942163384
1113	24	0.2602722897423817
1113	25	0.5946550906869277
1114	1	-0.7923358341326754
1114	2	-0.5353002463400998
1114	3	-0.4755542077176784
1114	4	-0.3026579593488705
1114	5	-3.1334088340651576
1114	6	-1.0437601960356204
1114	7	-0.6868358323026982
1114	8	-0.44780541719007516
1114	9	-0.1513128087578437
1114	10	-0.025679889696000657
1114	11	-0.4317428330166564
1114	12	-0.5557863862731622
1114	13	-0.4087063806076827
1114	14	-6.30689669433761
1114	15	-2.4365901345133807
1114	16	-1.2442327316101895
1114	17	-0.3416333258548363
1114	18	-6.5085526507128435
1114	19	-2.464277520245062
1114	20	-1.4424805866990766
1114	21	-1.0344044712203129
1114	22	-0.7704625486801433
1114	23	-0.3293164071060559
1114	24	-0.44440781366710524
1114	25	-0.32400351671899585
1115	1	-0.754160346348332
1115	2	-0.41203198428917176
1115	3	-0.5185178504789754
1115	4	-3.257746706531613
1115	5	-1.2728887481926208
1115	6	-0.7521273719157904
1115	7	-0.7174781158070545
1115	8	-0.6018342959721963
1115	9	-0.3272948083422307
1115	10	-0.7897780266554832
1115	11	-0.8145117945475633
1115	12	-0.797361596267713
1115	13	-0.8893233120198545
1115	14	-6.401856175961431
1115	15	-2.609370567250096
1115	16	-1.416465061733767
1115	17	-0.4135846986414933
1115	18	-0.3130543501203249
1115	19	-0.5440350536644232
1115	20	-0.6030609654232748
1115	21	-0.8278291482108813
1115	22	-2.0384845700791416
1115	23	-1.1315187816386185
1115	24	-0.9348558664363722
1115	25	-0.4968913331440419
1116	1	-0.28263646624750294
1116	2	0.005973455684550544
1116	3	-0.06089450043333855
1116	4	0.0955303512797272
1116	5	-0.6151297328816128
1116	6	-0.31862421324770906
1116	7	-0.18895099337022714
1116	8	-0.05216406041471089
1116	9	-0.13199681172487487
1116	10	0.30778908548283823
1116	11	-0.13683550876765338
1116	12	-0.4610582126836391
1116	13	-0.3330989162959881
1116	14	0.5248628285640606
1116	15	-0.02480308881407467
1116	16	0.08887973695023277
1116	17	0.4356273010158205
1116	18	0.34374630746348345
1116	19	0.10994665580107466
1116	20	-0.4028748915362641
1116	21	-0.41857320470774084
1116	22	-0.42503889060284195
1116	23	-0.9570365761790143
1116	24	-0.7334530071168146
1116	25	-0.18781988965046992
1201	1	0.7523263695282303
1201	2	0.8370942058293203
1201	3	0.7990705851506732
1201	4	1.1748111495247429
1201	5	1.0698690672206368
1201	6	0.8331154102452487
1201	7	0.7232665415702755
1201	8	0.8019653811633134
1201	9	1.0081944480634206
1201	10	1.3763728605176624
1201	11	0.8062273867421226
1201	12	0.8379776629547848
1201	13	0.7886349489920121
1201	14	1.5881135798043928
1201	15	1.2320508379817312
1201	16	1.3816498965366293
1201	17	1.605998434606171
1201	18	1.0072082472286183
1201	19	1.0405637097390823
1201	20	0.9700464812510958
1201	21	0.9285482471163675
1201	22	1.140166585365293
1201	23	1.4108139243870212
1201	24	1.1106193023733368
1201	25	1.569445197759471
1202	1	0.7867737950437821
1202	2	0.9593072066855477
1202	3	0.828326139738742
1202	4	1.2834498763594624
1202	5	0.964718284281318
1202	6	0.8052306343879323
1202	7	0.713282886063574
1202	8	0.8244820521836687
1202	9	1.0627283669686136
1202	10	1.3958412200457175
1202	11	1.0640658501783449
1202	12	0.8904506519109177
1202	13	0.9840154981379641
1202	14	1.8416546750487086
1202	15	1.382469790337808
1202	16	1.363999113597309
1202	17	1.8093094189764116
1202	18	2.0137347975065296
1202	19	1.526263003715023
1202	20	1.2986199570953545
1202	21	1.2008162133607243
1202	22	1.3979411039192868
1202	23	1.683948910672889
1202	24	1.3130542872079687
1202	25	1.8246530322320211
1203	1	0.5908889361411234
1203	2	0.6742236852732404
1203	3	0.5622996141021623
1203	4	0.9571102115570014
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2211	13	-0.756415420858005
2211	14	-0.32928054835430487
2211	15	-0.6531705017905054
2211	16	-7.147469495190929
2211	17	-2.3876183990970574
2211	18	-0.9449110087613306
2211	19	-0.8841990817819365
2211	20	-1.082491497293958
2211	21	-0.9162085901412311
2211	22	-0.798289787585702
2211	23	-0.41835451479832386
2211	24	-0.7896396136288715
2211	25	-8.106195340349217
2212	1	-1.3713249573234947
2212	2	-3.7641124999256554
2212	3	-1.630390177987291
2212	4	-3.6694344034850737
2212	5	-1.1869541850835112
2212	6	-1.0564472693500022
2212	7	-0.9511442800771235
2212	8	-0.7674852624404309
2212	9	-0.8509710099095855
2212	10	-0.29465384671372163
2212	11	-0.8726744267768396
2212	12	-0.898973999095837
2212	13	-1.0121471020096209
2212	14	-6.745691696570649
2212	15	-5.0480564851297665
2212	16	-2.680773890828064
2212	17	-7.627858157137435
2212	18	-3.0806314287275605
2212	19	-1.8781981493570825
2212	20	-1.8547343476142828
2212	21	-1.4819209595613643
2212	22	-1.3822374516487628
2212	23	-0.8320017531330492
2212	24	-1.253497840890013
2212	25	-1.0141276813420035
2213	1	-0.025980736282008365
2213	2	-0.008666392340376916
2213	3	0.010298590465829704
2213	4	0.3749004945600021
2213	5	0.2309875009633231
2213	6	-0.0827857288704692
2213	7	-0.13558638605355355
2213	8	-0.15352753017211995
2213	9	0.10846382267496324
2213	10	-3.837896492661791
2213	11	-1.4103894503096797
2213	12	-0.6940465713837156
2213	13	-0.5690331857729802
2213	14	0.24936931288911524
2213	15	-0.16621436808783585
2213	16	-0.028285753605892694
2213	17	0.4353936209582159
2213	18	0.5645846376356118
2213	19	-0.03451491924996475
2213	20	-0.4590842866607687
2213	21	-0.37566222138917027
2213	22	-0.5138638535639615
2213	23	-1.0225228916613247
2213	24	-0.351557073031195
2213	25	0.0021490973286830695
2214	1	-0.7869343421784194
2214	2	-0.49204487919568507
2214	3	-0.440074952306869
2214	4	-0.057204115660205446
2214	5	-3.18128969605198
2214	6	-1.0984284596095222
2214	7	-0.7838399937743503
2214	8	-0.5701923724540663
2214	9	-0.312304060856313
2214	10	-4.062460920808398
2214	11	-1.4972702997432066
2214	12	-0.9675241223296567
2214	13	-0.6402849873528993
2214	14	0.049077867429090816
2214	15	-0.19488138138760291
2214	16	-0.1484000513089896
2214	17	0.09201704970686819
2214	18	0.3914195125740392
2214	19	-0.3611970019675681
2214	20	-0.6253482203991183
2214	21	-0.6275436181381195
2214	22	-0.2903589368471273
2214	23	-0.032950618660393616
2214	24	-0.5064468801937352
2214	25	-0.05822754689805265
2215	1	-2.345389376161549
2215	2	-4.858807300466417
2215	3	-2.926559219960499
2215	4	-1.958040917846649
2215	5	-1.9192264544884807
2215	6	-2.1498340486466745
2215	7	-2.1378359099455437
2215	8	-2.433841193194091
2215	9	-8.412293855036449
2215	10	-4.052912250679256
2215	11	-3.2123844435837334
2215	12	-3.5565275015066327
2215	13	-2.8325522027324688
2215	14	-7.789481295933815
2215	15	-4.806815436782692
2215	16	-9.65053674475843
2215	17	-4.73805942640363
2215	18	-3.1000708598108133
2215	19	-3.565443373460912
2215	20	-3.417601027562405
2215	21	-3.519741268848435
2215	22	-9.534876573029159
2215	23	-10.623861060339763
2215	24	-6.6917345905105385
2215	25	-5.046105659391699
2216	1	-1.9918306259067329
2216	2	-2.008147628543469
2216	3	-1.8466660894458684
2216	4	-6.909492441422341
2216	5	-3.635038989102899
2216	6	-2.6466819761110862
2216	7	-2.216921926892404
2216	8	-7.909620245403911
2216	9	-3.9591079212543967
2216	10	-2.331472651602187
2216	11	-2.279616823378113
2216	12	-2.4860222888230084
2216	13	-2.2352252387531166
2216	14	-1.5239529829166258
2216	15	-2.2023401959024085
2216	16	-1.8656919346213456
2216	17	-1.5779397123751195
2216	18	-7.614620660377632
2216	19	-4.812583351921045
2216	20	-4.028366847367174
2216	21	-3.382538601080121
2216	22	-3.0545812928575593
2216	23	-2.22719176583351
2216	24	-2.5426718633181764
2216	25	-2.065499909293834
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	93094.0
3	3.0
4	801.0
5	4513.0
6	5728.0
7	5195.0
8	4751.0
9	4541.0
10	4253.0
11	3967.0
12	4170.0
13	4056.0
14	4073.0
15	4080.0
16	4185.0
17	4388.0
18	5115.0
19	6294.0
20	7925.0
21	10260.0
22	12983.0
23	15805.0
24	17945.0
25	21048.0
26	23379.0
27	26398.0
28	29512.0
29	35387.0
30	42764.0
31	50861.0
32	65283.0
33	84872.0
34	110951.0
35	148946.0
36	210612.0
37	304003.0
38	815777.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.71442571763928	18.50070083074083	12.350119084247831	25.434754367372054
2	14.266300071029045	20.24496581071421	41.59701760124376	23.891716517012984
3	18.25766744661448	25.075694779307007	29.9046955169804	26.76194225709811
4	11.340586500879981	16.86905442161322	39.50209138076844	32.28826769673836
5	12.593272475376342	38.04600065964174	36.43316291828061	12.927563946701312
6	31.44751110692454	37.36932327282375	18.455765475633026	12.727400144618686
7	27.693584652202226	31.656630384973656	22.780061405388878	17.869723557435243
8	24.946975691864267	36.424116197066134	21.18046375621878	17.44844435485082
9	26.569140379514295	14.896429704212938	20.27842637041392	38.256003545858846
10	13.869261528366017	28.73644293826858	35.82245226444399	21.57184326892141
11	34.1181594122701	22.507499526728896	24.804730162659638	18.569610898341367
12	23.15751867637254	24.764312484213544	31.915696344823978	20.162472494589938
13	28.76185595770835	21.908758561024307	25.793321206400726	23.53606427486662
14	20.452242073794817	21.7411051337444	28.850945428679744	28.95570736378104
15	23.289992571715715	30.273626677736264	23.463683336610515	22.9726974139375
16	22.158426402822894	27.855407738563855	27.849901386235576	22.13626447237768
17	21.623758428485207	28.162724497886675	28.330725247995687	21.882791825632427
18	21.538661089960794	27.249930025691384	29.838046453838572	21.373362430509253
19	23.350493600397574	27.107973152798586	27.63625209573099	21.90528115107285
20	23.649078541722986	27.189367936146454	27.777641245213395	21.383912276917165
21	23.232495172157034	26.757469104308264	27.10291645863211	22.907119264902594
22	23.200545117633162	27.657846739831506	27.185284008744826	21.95632413379051
23	22.268405794463128	27.359723657083563	27.86339412088637	22.508476427566933
24	22.974455428779	27.071837790743725	27.837656006329013	22.116050774148263
25	22.763202271354597	26.95217292953494	27.775211266836376	22.50941353227409
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	256.0
1	256.0
2	395.0
3	534.0
4	534.0
5	534.0
6	1072.5
7	1611.0
8	1611.0
9	1611.0
10	2548.0
11	3485.0
12	3485.0
13	3485.0
14	6654.5
15	9824.0
16	9824.0
17	9824.0
18	17970.0
19	26116.0
20	26116.0
21	26116.0
22	41570.5
23	57025.0
24	57025.0
25	57025.0
26	80465.5
27	103906.0
28	103906.0
29	103906.0
30	132934.0
31	161962.0
32	161962.0
33	161962.0
34	185802.0
35	209642.0
36	209642.0
37	209642.0
38	228724.5
39	247807.0
40	247807.0
41	247807.0
42	261283.5
43	274760.0
44	274760.0
45	274760.0
46	283663.0
47	292566.0
48	292566.0
49	292566.0
50	281331.5
51	270097.0
52	270097.0
53	270097.0
54	239681.5
55	209266.0
56	209266.0
57	209266.0
58	178755.0
59	148244.0
60	148244.0
61	148244.0
62	120770.5
63	93297.0
64	93297.0
65	93297.0
66	71708.5
67	50120.0
68	50120.0
69	50120.0
70	36364.0
71	22608.0
72	22608.0
73	22608.0
74	15923.5
75	9239.0
76	9239.0
77	9239.0
78	6443.5
79	3648.0
80	3648.0
81	3648.0
82	2464.0
83	1280.0
84	1280.0
85	1280.0
86	863.5
87	447.0
88	447.0
89	447.0
90	294.0
91	141.0
92	141.0
93	141.0
94	83.5
95	26.0
96	26.0
97	26.0
98	18.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18622168797926036
2	0.2664794591972949
3	0.2680263776901595
4	0.4717191451182437
5	0.264750550293505
6	0.018881505133494517
7	0.017789562667943026
8	0.015514682531377421
9	0.019336481160807637
10	0.020337428420896503
11	0.02029193081816519
12	0.02834500650160743
13	0.027526049652443815
14	0.025251169515878205
15	0.041402818485494
16	0.020746906845478315
17	0.041129832869106125
18	0.031074862665486154
19	0.02702557602239938
20	0.029118465748039737
21	0.03489666129491637
22	0.04372319622479092
23	0.02948244656989023
24	0.047863478073340314
25	0.03708054622601935
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2197918.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.18734350693045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.88290462852908	61.391669554072436
2	11.153648414741339	16.326117957704092
3	2.676424570541834	5.876412132439021
4	0.905209547173351	2.6499952829891598
5	0.4135984475521206	1.5135085827465105
6	0.23510593629919108	1.0324067352268442
7	0.14768832996245995	0.7566241575849212
8	0.10320777731096939	0.6042802440515759
9	0.08161525065267936	0.5375883046429687
>10	0.36775514652075014	4.660639996106167
>50	0.018860493645667466	0.9586396986320945
>100	0.011527577826128028	1.689886730603797
>500	0.0016359194962722596	0.8276469869087602
>1k	8.179597481355068E-4	1.1745836362916466
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	2959	0.13462740648195248	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2633	0.11979518799154472	No Hit
GTCCTACAGTGGACATTTCTAAATT	2602	0.11838476230687404	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2583	0.1175203078549791	No Hit
CCCATGTACTCTGCGTTGATACCAC	2420	0.11010419860977526	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.549760273131209E-5
6	0.0	0.0	0.0	0.0	4.549760273131209E-5
7	0.0	0.0	0.0	0.0	4.549760273131209E-5
8	0.0	0.0	0.0	0.0	4.549760273131209E-5
9	0.0	0.0	0.0	0.0	4.549760273131209E-5
10	0.0	0.0	0.0	4.549760273131209E-5	4.549760273131209E-5
11	0.0	0.0	0.0	4.549760273131209E-5	4.549760273131209E-5
12	0.0	0.0	0.0	4.549760273131209E-5	9.099520546262417E-5
13	0.0	0.0	0.0	4.549760273131209E-5	9.099520546262417E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	570	0.0	12.373166	4
ACCGTCC	70	1.1057122E-4	12.197125	8
TATACGG	55	0.0030439447	12.104222	5
ATTGCGA	55	0.0031016245	12.073921	8
AGGACCT	1125	0.0	10.989865	5
ACCGAGG	95	1.3821616E-5	10.984546	8
GGCGAGG	670	0.0	10.905798	19
GTCCTAC	1365	0.0	10.779922	1
CTACACT	360	0.0	10.589646	4
TGGCGAG	1480	0.0	10.579225	18
GTATAAT	340	0.0	10.398013	1
TCCTACA	1475	0.0	10.281509	2
TAGGTTG	150	2.4045221E-8	10.14449	5
GTCTAGA	245	0.0	10.139926	1
GTCTTAT	245	0.0	10.139926	1
GGACCTG	1125	0.0	10.118405	6
CGCAGAT	170	1.5988917E-9	10.044691	8
AGACCGT	85	6.6852383E-4	10.044005	6
TGTAGGA	1495	0.0	9.88877	2
GTATAAG	330	0.0	9.844474	1
>>END_MODULE