FastQCFastQC Report
Thu 26 May 2016
SRR1513124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513124_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197918
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38820.17662169380295353No Hit
GTACATGGGAAGCAGTGGTATCAAC34220.15569279654654997No Hit
CCCATGTACTCTGCGTTGATACCAC31600.1437724246309462No Hit
GTCCTACAGTGGACATTTCTAAATT27410.12470892908652642No Hit
TATCAACGCAGAGTACTTTTTTTTT26830.12207006812811033No Hit
GTCCTAAAGTGTGTATTTCTCATTT26410.12015916881339522No Hit
CTTTAGGACGTGAAATATGGCGAGG26360.11993168079973866No Hit
CTGTAGGACGTGGAATATGGCAAGA23400.10646439039127029No Hit
GGTATCAACGCAGAGTACTTTTTTT22620.10291557737822794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5900.012.4282584
ACCGTCC701.1044109E-412.1986658
GGCGAGG6650.011.840631519
ACCGTCT700.001507838510.8432578
TTAGGAC9850.010.8291173
AGGACGT13550.010.75285155
GGACGTG13550.010.7523616
GACGTGA8100.010.4287197
CTAGACA2300.010.3510344
GTAGGAC14450.010.3476813
TAGGACG14850.010.3245474
TAGACTG3050.010.3035215
TGTAGGA15450.010.2331822
ATAGGAC2151.8189894E-1210.1882733
AGGCGTG1804.0017767E-1010.01859957
CCTATAC2201.8189894E-129.9567213
GTCCTAA9300.09.9373051
AAGGCGT1553.918649E-89.8296946
GCGTGCA1554.15057E-89.79324159
GACGTGG6550.09.7086637
Produced by FastQC (version 0.11.5)