##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513123_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3364 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 11.62871581450654 2.0 2.0 26.0 2.0 31.0 2 11.65487514863258 2.0 2.0 26.0 2.0 31.0 3 11.610285374554103 2.0 2.0 26.0 2.0 31.0 4 13.112366230677765 2.0 2.0 30.0 2.0 35.0 5 12.913495838287753 2.0 2.0 30.0 2.0 35.0 6 12.703032104637337 2.0 2.0 30.0 2.0 35.0 7 12.293103448275861 2.0 2.0 30.0 2.0 35.0 8 11.935790725326992 2.0 2.0 29.0 2.0 35.0 9 12.186682520808562 2.0 2.0 30.0 2.0 37.0 10 12.072235434007135 2.0 2.0 29.0 2.0 37.0 11 11.999702734839477 2.0 2.0 30.0 2.0 37.0 12 11.774078478002378 2.0 2.0 28.0 2.0 37.0 13 11.819560047562426 2.0 2.0 29.0 2.0 37.0 14 11.486325802615934 2.0 2.0 27.0 2.0 38.0 15 11.557966706302022 2.0 2.0 27.0 2.0 38.0 16 11.567776456599287 2.0 2.0 27.0 2.0 38.0 17 11.326991676575505 2.0 2.0 27.0 2.0 38.0 18 11.17449464922711 2.0 2.0 25.0 2.0 38.0 19 11.41884661117717 2.0 2.0 27.0 2.0 38.0 20 11.383472057074911 2.0 2.0 27.0 2.0 38.0 21 11.286860879904875 2.0 2.0 27.0 2.0 38.0 22 11.041914387633769 2.0 2.0 26.0 2.0 38.0 23 10.953626634958383 2.0 2.0 25.0 2.0 38.0 24 10.874554102259216 2.0 2.0 25.0 2.0 38.0 25 10.700356718192628 2.0 2.0 24.0 2.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 19.749534970238095 1101 2 19.56129092261905 1101 3 19.570033482142854 1101 4 22.167503720238088 1101 5 21.28240327380952 1101 6 21.40292038690476 1101 7 22.06661086309524 1101 8 22.418954613095234 1101 9 24.273121279761902 1101 10 24.466648065476186 1101 11 25.61166294642857 1101 12 24.56928943452381 1101 13 25.565066964285712 1101 14 24.72935267857143 1101 15 25.87416294642857 1101 16 26.39071800595238 1101 17 26.759709821428572 1101 18 26.995145089285714 1101 19 27.22758556547619 1101 20 23.433854166666663 1101 21 25.66915922619048 1101 22 22.652046130952385 1101 23 25.522302827380948 1101 24 22.685323660714282 1101 25 27.50645461309524 1102 1 0.04953497023809739 1102 2 2.86129092261905 1102 3 4.4700334821428545 1102 4 4.16750372023809 1102 5 -1.3175967261904802 1102 6 4.002920386904764 1102 7 -1.733389136904762 1102 8 3.818954613095233 1102 9 -1.7268787202380977 1102 10 1.4666480654761873 1102 11 0.4116629464285708 1102 12 0.5692894345238102 1102 13 1.1650669642857139 1102 14 -1.4706473214285687 1102 15 0.3741629464285676 1102 16 1.0907180059523771 1102 17 -0.940290178571427 1102 18 -4.304854910714289 1102 19 -1.1724144345238106 1102 20 -0.36614583333333606 1102 21 2.1691592261904784 1102 22 0.7520461309523832 1102 23 3.2223028273809486 1102 24 3.585323660714282 1102 25 2.7064546130952394 1103 1 3.7495349702380967 1103 2 2.5612909226190492 1103 3 3.570033482142856 1103 4 4.16750372023809 1103 5 4.2824032738095195 1103 6 4.402920386904762 1103 7 3.0666108630952387 1103 8 5.418954613095234 1103 9 5.023121279761902 1103 10 4.466648065476187 1103 11 4.61166294642857 1103 12 6.56928943452381 1103 13 6.565066964285714 1103 14 4.479352678571431 1103 15 6.874162946428568 1103 16 6.390718005952378 1103 17 8.259709821428572 1103 18 5.745145089285712 1103 19 7.47758556547619 1103 20 6.683854166666665 1103 21 7.419159226190478 1103 22 7.6520461309523835 1103 23 5.522302827380949 1103 24 8.185323660714284 1103 25 4.25645461309524 1104 1 -2.4171316964285694 1104 2 -2.9387090773809508 1104 3 -1.263299851190478 1104 4 -1.8324962797619104 1104 5 -1.7175967261904805 1104 6 -2.9304129464285715 1104 7 -0.9333891369047613 1104 8 -0.5810453869047656 1104 9 -0.7268787202380977 1104 10 -1.5333519345238127 1104 11 -0.7216703869047638 1104 12 -1.4307105654761898 1104 13 -3.101599702380952 1104 14 0.06268601190476453 1104 15 -0.6258370535714324 1104 16 -1.1092819940476222 1104 17 -0.07362351190476168 1104 18 -8.504854910714288 1104 19 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24 6.018656994047616 2214 25 7.00645461309524 2215 1 -3.179036458333332 2215 2 -7.7958519345238075 2215 3 -5.6442522321428585 2215 4 -6.689639136904768 2215 5 -5.431882440476194 2215 6 -4.311365327380951 2215 7 -3.5048177083333325 2215 8 -3.295331101190479 2215 9 -4.441164434523811 2215 10 -4.9619233630952415 2215 11 -4.816908482142859 2215 12 -7.002139136904761 2215 13 -7.434933035714286 2215 14 -6.842075892857141 2215 15 -6.482979910714289 2215 16 -5.966424851190479 2215 17 -6.168861607142857 2215 18 -3.2191406250000014 2215 19 -8.343843005952381 2215 20 -2.994717261904764 2215 21 -4.402269345238093 2215 22 -6.133668154761902 2215 23 -5.834840029761907 2215 24 -4.3861049107142875 2215 25 -3.6364025297619023 2216 1 -3.9171316964285694 2216 2 1.5612909226190492 2216 3 1.236700148809522 2216 4 -8.33249627976191 2216 5 -2.3842633928571466 2216 6 0.4029203869047624 2216 7 1.3999441964285726 2216 8 -4.081045386904766 2216 9 -2.393545386904764 2216 10 -0.7000186011904788 2216 11 2.778329613095238 2216 12 -0.4307105654761898 2216 13 2.398400297619048 2216 14 3.8960193452380985 2216 15 1.7074962797619015 2216 16 4.390718005952378 2216 17 4.093043154761904 2216 18 1.495145089285712 2216 19 4.394252232142858 2216 20 4.6005208333333325 2216 21 3.8358258928571463 2216 22 1.3187127976190496 2216 23 3.3556361607142815 2216 24 4.685323660714284 2216 25 -0.8268787202380938 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 1965.0 3 0.0 4 12.0 5 64.0 6 62.0 7 56.0 8 35.0 9 26.0 10 23.0 11 25.0 12 24.0 13 20.0 14 12.0 15 15.0 16 14.0 17 9.0 18 19.0 19 20.0 20 25.0 21 30.0 22 24.0 23 25.0 24 29.0 25 28.0 26 33.0 27 29.0 28 35.0 29 44.0 30 40.0 31 50.0 32 37.0 33 58.0 34 80.0 35 104.0 36 91.0 37 94.0 38 107.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.281807372175976 17.746730083234244 12.901307966706302 26.07015457788347 2 15.572792362768498 19.48090692124105 39.88663484486874 25.059665871121716 3 17.91044776119403 24.089552238805968 28.65671641791045 29.34328358208955 4 12.069481880802634 15.124288709194369 38.81401617250674 33.99221323749625 5 12.507462686567164 38.59701492537314 35.582089552238806 13.313432835820896 6 31.212841854934602 37.96076099881094 17.271105826397147 13.555291319857313 7 29.369797859690845 30.231866825208087 22.205707491082045 18.192627824019027 8 24.55410225921522 36.92033293697979 20.5410225921522 17.984542211652794 9 25.862068965517242 14.595719381688465 19.67895362663496 39.86325802615933 10 14.328180737217597 28.5077288941736 34.36385255648038 22.80023781212842 11 34.869203329369796 23.097502972651604 23.305588585017837 18.72770511296076 12 22.502972651605234 24.97027348394768 30.529131985731272 21.997621878715815 13 29.90487514863258 21.67063020214031 24.346016646848987 24.07847800237812 14 22.11652794292509 20.98692033293698 27.2294887039239 29.66706302021403 15 22.681331747919145 31.00475624256837 22.11652794292509 24.197384066587396 16 22.502972651605234 26.902497027348392 27.85374554102259 22.74078478002378 17 22.651605231866824 27.49702734839477 26.159334126040427 23.69203329369798 18 22.205707491082045 26.248513674197383 28.240190249702735 23.305588585017837 19 22.651605231866824 27.25921521997622 27.46730083234245 22.621878715814507 20 23.69203329369798 27.705112960760996 25.653983353151013 22.94887039239001 21 23.365041617122472 25.505350772889418 25.089179548156952 26.040428061831157 22 23.275862068965516 26.486325802615934 27.199762187871578 23.03804994054697 23 23.959571938168846 26.81331747919144 26.337693222354343 22.889417360285375 24 22.450193279809692 26.55367231638418 27.326791555159087 23.66934284864704 25 22.681331747919145 25.921521997621877 27.88347205707491 23.513674197384066 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 1.0 2 0.5 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 2.0 9 2.0 10 3.0 11 4.0 12 4.0 13 4.0 14 8.0 15 12.0 16 12.0 17 12.0 18 24.5 19 37.0 20 37.0 21 37.0 22 56.0 23 75.0 24 75.0 25 75.0 26 105.0 27 135.0 28 135.0 29 135.0 30 170.5 31 206.0 32 206.0 33 206.0 34 241.0 35 276.0 36 276.0 37 276.0 38 319.0 39 362.0 40 362.0 41 362.0 42 387.5 43 413.0 44 413.0 45 413.0 46 424.0 47 435.0 48 435.0 49 435.0 50 423.0 51 411.0 52 411.0 53 411.0 54 393.5 55 376.0 56 376.0 57 376.0 58 315.0 59 254.0 60 254.0 61 254.0 62 226.0 63 198.0 64 198.0 65 198.0 66 143.5 67 89.0 68 89.0 69 89.0 70 66.5 71 44.0 72 44.0 73 44.0 74 31.0 75 18.0 76 18.0 77 18.0 78 14.5 79 11.0 80 11.0 81 11.0 82 7.5 83 4.0 84 4.0 85 4.0 86 2.0 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.356718192627824 3 0.4161712247324614 4 0.7431629013079667 5 0.4161712247324614 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.029726516052318665 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3364.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.76218787158146 #Duplication Level Percentage of deduplicated Percentage of total 1 99.880810488677 99.64328180737218 2 0.02979737783075089 0.05945303210463733 3 0.05959475566150178 0.178359096313912 4 0.02979737783075089 0.11890606420927466 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACATGGGAAG 4 0.11890606420927466 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE