FastQCFastQC Report
Thu 26 May 2016
SRR1513123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364
Sequences flagged as poor quality0
Sequence length25
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCG260.7728894173602854No Hit
TCTCCGAGCCCACGAGACGGACTCC230.6837098692033293No Hit
TCTCCGAGCCCACGAGACAGGCAGA210.6242568370986921No Hit
TCTCCGAGCCCACGAGACGTAGAGG150.44589774078478No Hit
CTCCGAGCCCACGAGACTAAGGCGA100.2972651605231867No Hit
CTCCGAGCCCACGAGACGTAGAGGA70.2080856123662307No Hit
CTCCGAGCCCACGAGACAGGCAGAA60.178359096313912No Hit
TCTCCGAGCCCACGAGACTCCTGAG50.14863258026159334No Hit
CTCCGAGCCCACGAGACGGACTCCT50.14863258026159334No Hit
CATCTCCGAGCCCACGAGACTAAGG40.11890606420927466No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)