##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513123_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3364 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 16.184304399524375 16.0 2.0 30.0 2.0 31.0 2 16.366230677764566 18.0 2.0 30.0 2.0 31.0 3 16.51783590963139 22.0 2.0 30.0 2.0 31.0 4 18.728894173602853 25.0 2.0 35.0 2.0 37.0 5 16.95986920332937 10.0 2.0 35.0 2.0 37.0 6 16.106718192627824 2.0 2.0 33.0 2.0 37.0 7 15.192330558858501 2.0 2.0 33.0 2.0 37.0 8 14.97384066587396 2.0 2.0 33.0 2.0 37.0 9 15.306183115338882 2.0 2.0 34.0 2.0 39.0 10 15.063912009512485 2.0 2.0 34.0 2.0 39.0 11 14.816884661117717 2.0 2.0 34.0 2.0 39.0 12 14.785077288941736 2.0 2.0 34.0 2.0 39.0 13 14.59423305588585 2.0 2.0 34.0 2.0 39.0 14 14.551426872770511 2.0 2.0 34.0 2.0 40.0 15 14.589179548156956 2.0 2.0 34.0 2.0 40.0 16 14.429250891795482 2.0 2.0 34.0 2.0 40.0 17 14.367122473246136 2.0 2.0 34.0 2.0 40.0 18 14.200356718192628 2.0 2.0 34.0 2.0 40.0 19 14.25653983353151 2.0 2.0 34.0 2.0 40.0 20 14.144768133174791 2.0 2.0 34.0 2.0 40.0 21 13.934007134363853 2.0 2.0 33.0 2.0 40.0 22 13.98127229488704 2.0 2.0 34.0 2.0 40.0 23 13.859393579072533 2.0 2.0 34.0 2.0 40.0 24 13.728299643281808 2.0 2.0 33.0 2.0 40.0 25 13.673008323424495 2.0 2.0 33.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.672860863095238 1101 2 14.466015625 1101 3 14.398921130952381 1101 4 15.66073288690476 1101 5 17.16127232142857 1101 6 19.066964285714285 1101 7 19.68141741071428 1101 8 19.22862723214286 1101 9 21.813597470238093 1101 10 17.74542410714286 1101 11 20.605896577380953 1101 12 22.627752976190475 1101 13 19.00498511904762 1101 14 21.12911086309524 1101 15 21.564936755952385 1101 16 19.478924851190474 1101 17 17.109523809523814 1101 18 22.022395833333334 1101 19 20.32042410714286 1101 20 20.91502976190476 1101 21 23.043917410714286 1101 22 23.408872767857137 1101 23 23.066908482142857 1101 24 24.20379464285714 1101 25 23.82799479166666 1102 1 -0.02713913690476133 1102 2 -2.833984375 1102 3 -0.30107886904761827 1102 4 -2.5392671130952387 1102 5 1.561272321428568 1102 6 -2.1330357142857146 1102 7 -0.9185825892857231 1102 8 -6.97137276785714 1102 9 -6.986402529761907 1102 10 -2.75457589285714 1102 11 -1.3941034226190467 1102 12 -6.572247023809524 1102 13 -2.0950148809523785 1102 14 -2.8708891369047613 1102 15 -6.235063244047616 1102 16 -2.121075148809526 1102 17 -0.7904761904761859 1102 18 -0.8776041666666661 1102 19 -0.6795758928571409 1102 20 -1.1849702380952412 1102 21 -0.5560825892857135 1102 22 -2.4911272321428637 1102 23 -3.8330915178571434 1102 24 -4.996205357142861 1102 25 -0.2720052083333364 1103 1 -0.827139136904762 1103 2 -1.5339843749999993 1103 3 -0.851078869047619 1103 4 -1.3392671130952394 1103 5 0.1612723214285694 1103 6 1.0669642857142847 1103 7 1.6814174107142783 1103 8 2.228627232142859 1103 9 3.3135974702380935 1103 10 1.9954241071428598 1103 11 3.3558965773809533 1103 12 0.6277529761904752 1103 13 2.754985119047621 1103 14 1.1291108630952387 1103 15 3.3149367559523846 1103 16 1.4789248511904738 1103 17 -1.1404761904761855 1103 18 2.5223958333333343 1103 19 3.070424107142859 1103 20 3.9150297619047585 1103 21 3.793917410714286 1103 22 4.408872767857137 1103 23 3.566908482142857 1103 24 4.453794642857138 1103 25 3.3279947916666632 1104 1 -1.827139136904762 1104 2 -4.033984374999999 1104 3 1.2322544642857132 1104 4 -2.3392671130952394 1104 5 -3.1720610119047645 1104 6 4.7336309523809526 1104 7 5.681417410714278 1104 8 4.228627232142859 1104 9 1.4802641369047613 1104 10 5.24542410714286 1104 11 7.939229910714285 1104 12 7.961086309523807 1104 13 5.171651785714289 1104 14 5.129110863095239 1104 15 3.2316034226190524 1104 16 -1.1877418154761923 1104 17 2.2761904761904823 1104 18 6.522395833333334 1104 19 7.653757440476191 1104 20 3.0816964285714263 1104 21 0.37725074404761827 1104 22 -7.091127232142863 1104 23 -5.099758184523809 1104 24 -4.462872023809528 1104 25 -4.338671875000003 1105 1 5.672860863095238 1105 2 5.716015625000001 1105 3 5.398921130952381 1105 4 5.160732886904761 1105 5 7.911272321428569 1105 6 9.316964285714285 1105 7 10.431417410714278 1105 8 10.478627232142859 1105 9 12.563597470238093 1105 10 11.99542410714286 1105 11 9.105896577380953 1105 12 11.627752976190475 1105 13 11.754985119047621 1105 14 12.379110863095239 1105 15 12.314936755952385 1105 16 12.728924851190474 1105 17 12.109523809523814 1105 18 9.022395833333334 1105 19 8.070424107142859 1105 20 8.915029761904758 1105 21 11.293917410714286 1105 22 11.408872767857137 1105 23 12.066908482142857 1105 24 9.953794642857138 1105 25 12.327994791666663 1106 1 4.672860863095238 1106 2 5.216015625000001 1106 3 3.398921130952381 1106 4 5.660732886904761 1106 5 0.1612723214285694 1106 6 0.5669642857142847 1106 7 1.6814174107142783 1106 8 1.728627232142859 1106 9 -0.4364025297619065 1106 10 1.4954241071428598 1106 11 3.1058965773809533 1106 12 3.627752976190475 1106 13 3.504985119047621 1106 14 3.8791108630952387 1106 15 4.564936755952385 1106 16 4.228924851190474 1106 17 4.3595238095238145 1106 18 2.5223958333333343 1106 19 2.820424107142859 1106 20 4.6650297619047585 1106 21 5.293917410714286 1106 22 4.658872767857137 1106 23 4.816908482142857 1106 24 5.453794642857138 1106 25 5.577994791666663 1107 1 0.7728608630952394 1107 2 -3.4339843749999996 1107 3 -0.10107886904761898 1107 4 0.26073288690476204 1107 5 -7.238727678571431 1107 6 2.8669642857142854 1107 7 -1.518582589285721 1107 8 -0.5713727678571416 1107 9 4.213597470238092 1107 10 4.0454241071428605 1107 11 -1.3941034226190467 1107 12 -6.972247023809524 1107 13 -7.0950148809523785 1107 14 -7.070889136904761 1107 15 -6.635063244047615 1107 16 -6.921075148809527 1107 17 -6.990476190476185 1107 18 -7.277604166666666 1107 19 -7.079575892857141 1107 20 -6.784970238095241 1107 21 -6.5560825892857135 1107 22 -6.491127232142864 1107 23 -6.833091517857143 1107 24 -6.596205357142862 1107 25 -5.872005208333336 1108 1 -0.9938058035714299 1108 2 1.9660156250000007 1108 3 -1.2677455357142868 1108 4 2.3273995535714285 1108 5 3.4946056547619015 1108 6 -4.266369047619049 1108 7 -1.4852492559523878 1108 8 0.7286272321428591 1108 9 -2.1864025297619065 1108 10 2.078757440476192 1108 11 0.9392299107142854 1108 12 -0.20558035714285694 1108 13 -1.8283482142857128 1108 14 -1.0375558035714274 1108 15 2.5649367559523846 1108 16 -0.5210751488095262 1108 17 -1.5571428571428516 1108 18 2.0223958333333343 1108 19 1.320424107142859 1108 20 2.5816964285714263 1108 21 -1.9560825892857139 1108 22 -3.0911272321428633 1108 23 -2.766424851190477 1108 24 -3.2962053571428616 1108 25 -1.3386718750000028 1109 1 -3.2271391369047624 1109 2 -3.833984375 1109 3 -3.5010788690476193 1109 4 -4.339267113095239 1109 5 -2.8387276785714306 1109 6 -1.9330357142857153 1109 7 -1.3185825892857217 1109 8 -0.7713727678571409 1109 9 -0.3864025297619058 1109 10 0.04542410714286049 1109 11 0.40589657738095397 1109 12 0.027752976190473788 1109 13 0.3049851190476218 1109 14 0.7291108630952401 1109 15 0.9649367559523832 1109 16 0.8789248511904724 1109 17 1.2095238095238159 1109 18 1.1223958333333357 1109 19 0.7204241071428577 1109 20 0.4150297619047585 1109 21 1.2439174107142854 1109 22 1.3088727678571352 1109 23 1.1669084821428584 1109 24 1.8037946428571399 1109 25 1.7279947916666618 1110 1 0.9728608630952387 1110 2 0.5660156250000021 1110 3 -3.5010788690476193 1110 4 -1.739267113095238 1110 5 0.7612723214285708 1110 6 1.6669642857142861 1110 7 3.2814174107142797 1110 8 3.8286272321428605 1110 9 -1.7864025297619062 1110 10 4.24542410714286 1110 11 1.6058965773809533 1110 12 2.8277529761904745 1110 13 2.504985119047621 1110 14 1.5291108630952372 1110 15 4.164936755952386 1110 16 4.2789248511904745 1110 17 0.20952380952381588 1110 18 0.7223958333333336 1110 19 2.5204241071428584 1110 20 0.6150297619047578 1110 21 2.843917410714287 1110 22 5.108872767857136 1110 23 6.166908482142858 1110 24 4.80379464285714 1110 25 1.5279947916666625 1111 1 -2.827139136904762 1111 2 1.5660156250000021 1111 3 0.49892113095238244 1111 4 1.0607328869047592 1111 5 2.561272321428568 1111 6 3.666964285714286 1111 7 4.081417410714277 1111 8 0.42862723214285836 1111 9 5.013597470238093 1111 10 5.845424107142861 1111 11 0.40589657738095397 1111 12 0.4277529761904759 1111 13 0.3049851190476218 1111 14 0.5291108630952372 1111 15 0.9649367559523832 1111 16 0.8789248511904724 1111 17 1.2095238095238159 1111 18 1.1223958333333357 1111 19 0.7204241071428577 1111 20 0.8150297619047571 1111 21 1.6439174107142875 1111 22 1.508872767857138 1111 23 0.9669084821428555 1111 24 1.2037946428571384 1111 25 1.7279947916666618 1112 1 -1.827139136904762 1112 2 -2.0339843749999993 1112 3 -3.101078869047619 1112 4 0.46073288690476133 1112 5 -3.6387276785714313 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5.7957775297619065 2216 15 2.5649367559523846 2216 16 6.478924851190474 2216 17 6.942857142857147 2216 18 6.855729166666666 2216 19 5.820424107142859 2216 20 6.081696428571426 2216 21 2.210584077380954 2216 22 6.5755394345238045 2216 23 2.233575148809525 2216 24 0.7037946428571384 2216 25 3.494661458333331 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 1407.0 3 0.0 4 21.0 5 145.0 6 146.0 7 100.0 8 67.0 9 45.0 10 40.0 11 33.0 12 25.0 13 24.0 14 25.0 15 12.0 16 11.0 17 19.0 18 8.0 19 13.0 20 22.0 21 18.0 22 23.0 23 30.0 24 27.0 25 28.0 26 26.0 27 22.0 28 28.0 29 28.0 30 37.0 31 57.0 32 63.0 33 68.0 34 78.0 35 112.0 36 132.0 37 184.0 38 240.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.020226055919096 14.485425342058297 14.96133254015467 34.53301606186794 2 10.916121356335514 17.19214753123141 36.25817965496728 35.63355145746579 3 11.801426872770511 20.154577883472058 30.112960760998813 37.93103448275862 4 7.8478002378121285 12.395957193816885 33.02615933412604 46.730083234244944 5 12.455410225921522 36.1474435196195 34.18549346016647 17.211652794292508 6 34.31460005947071 36.366339577757955 16.08682723758549 13.232233125185846 7 27.080856123662304 33.680142687277055 20.95719381688466 18.281807372175983 8 27.199762187871578 34.30439952437575 18.192627824019027 20.303210463733652 9 23.335315101070154 14.001189060642094 16.676575505350772 45.98692033293698 10 13.733650416171225 27.794292508917955 31.896551724137932 26.575505350772886 11 30.737217598097505 22.413793103448278 23.48394768133175 23.365041617122472 12 20.422116527942926 26.605231866825207 28.864447086801427 24.10820451843044 13 27.140309155766946 22.32461355529132 23.602853745541022 26.932223543400713 14 23.127229488703925 21.135552913198573 24.97027348394768 30.76694411414982 15 22.97859690844233 30.707491082045184 21.70035671819263 24.61355529131986 16 25.0 26.426872770511295 23.989298454221164 24.583828775267538 17 19.922688076122512 31.57894736842105 25.6021409455843 22.896223609872138 18 20.071364852809992 27.178114778471603 25.869759143621767 26.880761225096638 19 21.938168846611177 28.56718192627824 26.932223543400713 22.56242568370987 20 24.10820451843044 28.032104637336502 25.44589774078478 22.413793103448278 21 23.067776456599287 27.2294887039239 24.10820451843044 25.59453032104637 22 23.365041617122472 26.456599286563616 25.267538644470868 24.910820451843044 23 21.201308355634847 26.821290514421648 25.393993458221825 26.583407671721677 24 22.384066587395957 25.62425683709869 27.170035671819264 24.821640903686088 25 22.97859690844233 26.486325802615934 25.743162901307965 24.79191438763377 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 1.0 2 0.5 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 2.0 9 2.0 10 1.5 11 1.0 12 1.0 13 1.0 14 4.5 15 8.0 16 8.0 17 8.0 18 20.0 19 32.0 20 32.0 21 32.0 22 40.5 23 49.0 24 49.0 25 49.0 26 71.5 27 94.0 28 94.0 29 94.0 30 131.0 31 168.0 32 168.0 33 168.0 34 216.0 35 264.0 36 264.0 37 264.0 38 299.5 39 335.0 40 335.0 41 335.0 42 350.5 43 366.0 44 366.0 45 366.0 46 403.5 47 441.0 48 441.0 49 441.0 50 441.5 51 442.0 52 442.0 53 442.0 54 391.5 55 341.0 56 341.0 57 341.0 58 326.5 59 312.0 60 312.0 61 312.0 62 297.0 63 282.0 64 282.0 65 282.0 66 208.5 67 135.0 68 135.0 69 135.0 70 100.0 71 65.0 72 65.0 73 65.0 74 41.5 75 18.0 76 18.0 77 18.0 78 13.0 79 8.0 80 8.0 81 8.0 82 4.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05945303210463733 2 0.05945303210463733 3 0.0 4 0.0 5 0.0 6 0.029726516052318665 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.029726516052318665 18 0.029726516052318665 19 0.0 20 0.0 21 0.0 22 0.0 23 0.029726516052318665 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3364.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.16527942925089 #Duplication Level Percentage of deduplicated Percentage of total 1 99.258114374034 95.45184304399524 2 0.3400309119010819 0.6539833531510106 3 0.09273570324574962 0.267538644470868 4 0.030911901081916538 0.11890606420927466 5 0.061823802163833076 0.2972651605231867 6 0.030911901081916538 0.178359096313912 7 0.030911901081916538 0.2080856123662307 8 0.0 0.0 9 0.0 0.0 >10 0.1545595054095827 2.8240190249702737 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTCCGAGCCCACGAGACTAAGGCG 26 0.7728894173602854 No Hit TCTCCGAGCCCACGAGACGGACTCC 23 0.6837098692033293 No Hit TCTCCGAGCCCACGAGACAGGCAGA 21 0.6242568370986921 No Hit TCTCCGAGCCCACGAGACGTAGAGG 15 0.44589774078478 No Hit CTCCGAGCCCACGAGACTAAGGCGA 10 0.2972651605231867 No Hit CTCCGAGCCCACGAGACGTAGAGGA 7 0.2080856123662307 No Hit CTCCGAGCCCACGAGACAGGCAGAA 6 0.178359096313912 No Hit TCTCCGAGCCCACGAGACTCCTGAG 5 0.14863258026159334 No Hit CTCCGAGCCCACGAGACGGACTCCT 5 0.14863258026159334 No Hit CATCTCCGAGCCCACGAGACTAAGG 4 0.11890606420927466 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE