FastQCFastQC Report
Thu 26 May 2016
SRR1513122_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513122_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2035380
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22670.11137969322681758No Hit
GTCCTACAGTGGACATTTCTAAATT22110.1086283642366536No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGA500.001512603613.2850346
TAGGACC6550.013.0821494
TCCAACG1251.03682396E-1012.91022118
GGCGAGG5500.011.73685419
GACGTGA6000.011.5461797
TTAGGAC8600.011.2922273
TGTAGGA14100.010.8780842
GGACGTG11000.010.8695746
AGGCGTG1053.511308E-610.8457257
GTCTAGG2200.010.8432651
TGGCGAG12400.010.79424218
ACGTGAA7200.010.6759988
GTCCTAC14050.010.5947571
GTCCTAA7500.010.5598931
CGTGAAA7300.010.40189
AGGACCT11450.010.381455
GTAGGAC13500.010.3671993
AGGACGT11350.010.3053495
CTTACAC3050.010.3012983
ACCGTGC750.0026719310.1224288
Produced by FastQC (version 0.11.5)