##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513122_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2035380
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.48605370987236	31.0	30.0	34.0	25.0	34.0
2	29.392964458725153	31.0	30.0	34.0	23.0	34.0
3	29.57951045996325	31.0	30.0	34.0	23.0	34.0
4	32.68937643093673	37.0	33.0	37.0	24.0	37.0
5	32.83640352170111	37.0	35.0	37.0	25.0	37.0
6	33.15740304021853	37.0	35.0	37.0	27.0	37.0
7	33.116094783283714	37.0	35.0	37.0	26.0	37.0
8	33.024524167477324	37.0	35.0	37.0	26.0	37.0
9	34.27378523911997	39.0	35.0	39.0	25.0	39.0
10	33.89042144464425	39.0	34.0	39.0	21.0	39.0
11	34.286426121903524	39.0	35.0	39.0	26.0	39.0
12	34.279082038734785	39.0	35.0	39.0	26.0	39.0
13	34.31061030372707	39.0	35.0	39.0	26.0	39.0
14	34.61501341272883	40.0	34.0	41.0	17.0	41.0
15	35.00808988984858	40.0	35.0	41.0	22.0	41.0
16	34.83535703406735	40.0	34.0	41.0	19.0	41.0
17	34.48148306458745	39.0	34.0	41.0	17.0	41.0
18	34.22975758826362	39.0	33.0	41.0	17.0	41.0
19	34.83685945621948	40.0	34.0	41.0	19.0	41.0
20	35.04056539810748	40.0	35.0	41.0	23.0	41.0
21	35.084632353663686	40.0	36.0	41.0	24.0	41.0
22	34.79430966207784	40.0	35.0	41.0	18.0	41.0
23	34.32686525366271	40.0	34.0	41.0	11.0	41.0
24	34.57191826587664	40.0	34.0	41.0	18.0	41.0
25	34.19266033860999	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8535808236110043
1101	2	1.012555804723
1101	3	0.9549750951524274
1101	4	1.3277486168278259
1101	5	1.1271078055461174
1101	6	0.856897719973901
1101	7	0.7763079984282584
1101	8	0.8605496762802289
1101	9	1.333520312950462
1101	10	1.7056353450567698
1101	11	1.2813478256543291
1101	12	1.1079462753184117
1101	13	1.0291927838455805
1101	14	1.9542358627246657
1101	15	1.533313716900146
1101	16	1.748785177175705
1101	17	2.021287594787914
1101	18	1.9696058230964297
1101	19	1.542385237614461
1101	20	1.2066655273709515
1101	21	1.216765447613568
1101	22	1.3765738409951567
1101	23	1.682024550206222
1101	24	1.3017989901901785
1101	25	1.7051918600629463
1102	1	0.727450897085923
1102	2	0.92507644972736
1102	3	0.8506660745750949
1102	4	1.1310307111138584
1102	5	1.0657674632436667
1102	6	0.7897553860969779
1102	7	0.7466700538330571
1102	8	0.8637439376078859
1102	9	1.1319804913990197
1102	10	1.3014622384107142
1102	11	0.8742595734071585
1102	12	0.8903894323453798
1102	13	0.8317150473236339
1102	14	1.8274726492120195
1102	15	1.4589892403722757
1102	16	1.539673927458466
1102	17	1.9091020022325154
1102	18	2.1065893667209252
1102	19	1.4279303745742453
1102	20	1.2273350454538487
1102	21	1.211099086027616
1102	22	1.2552659249551397
1102	23	1.6099275099308414
1102	24	1.353633496995812
1102	25	1.6702597081838633
1103	1	0.6092208618050137
1103	2	0.9372532958678335
1103	3	0.6776886195923133
1103	4	1.0246618259623759
1103	5	0.9631747157957804
1103	6	0.6125005320359094
1103	7	0.6175776328862526
1103	8	0.6521566725594781
1103	9	0.9909137019616807
1103	10	1.2405897523516032
1103	11	0.7860770334636271
1103	12	0.8243901746068332
1103	13	0.8094082114714638
1103	14	1.6142505298206729
1103	15	1.2432612838932258
1103	16	1.3272673560956676
1103	17	1.5847750877382154
1103	18	1.9247935637866433
1103	19	1.3352303750440342
1103	20	0.9578687690225252
1103	21	1.011617259688606
1103	22	1.2073449617064753
1103	23	1.7111902939750365
1103	24	1.356849180322648
1103	25	1.5192043345397792
1104	1	0.7552574513589398
1104	2	1.0815211247356125
1104	3	0.8164112362665037
1104	4	1.3313862119467288
1104	5	1.2210742002964565
1104	6	0.9349516310321135
1104	7	0.8044312554389137
1104	8	0.9089642582478419
1104	9	1.1840486503247476
1104	10	1.363815519808199
1104	11	1.019077964824099
1104	12	0.8550252488913017
1104	13	0.8111572826294164
1104	14	1.7823436951733314
1104	15	1.3709475079087667
1104	16	1.3024903285550522
1104	17	1.8714346539641724
1104	18	1.9342423394185175
1104	19	1.4057025848776235
1104	20	1.0228665573848303
1104	21	1.0763279472560896
1104	22	1.4410204654607668
1104	23	1.615169959729272
1104	24	1.3916114544540008
1104	25	1.7319614088041035
1105	1	0.5487674515481089
1105	2	0.8405799822816604
1105	3	0.7634190386063757
1105	4	1.0573156247091404
1105	5	1.0044011047115546
1105	6	0.7547459001655454
1105	7	0.6783033732433807
1105	8	0.7873517886869692
1105	9	0.9076729737690599
1105	10	1.3836759880655691
1105	11	0.786331294268102
1105	12	0.8606900991029889
1105	13	0.7179524070328682
1105	14	1.5174991310635804
1105	15	1.2040567201053847
1105	16	1.4714041922910468
1105	17	1.7097681504193147
1105	18	1.3644672839319725
1105	19	1.1363377647406594
1105	20	1.1305763884573494
1105	21	1.0306358387966341
1105	22	1.1734895547923898
1105	23	1.557133414413343
1105	24	1.4681063515743986
1105	25	1.6609108504568937
1106	1	0.4622137231222716
1106	2	0.732094654905687
1106	3	0.67212712609291
1106	4	1.0707646336363013
1106	5	0.9152784757833743
1106	6	0.5936401340642092
1106	7	0.6076985253454552
1106	8	0.6675497496611058
1106	9	0.8823272822482409
1106	10	0.7933202228110687
1106	11	0.6229223980905871
1106	12	0.7214476354523356
1106	13	0.548695645976963
1106	14	1.5221132645389304
1106	15	1.017142475881677
1106	16	1.1770815264864183
1106	17	1.5063976540859443
1106	18	1.6919833301762068
1106	19	1.0281187570924573
1106	20	0.7874402186953233
1106	21	0.6086677479099194
1106	22	0.7634102731763477
1106	23	1.2044727210354225
1106	24	0.8369812381550616
1106	25	1.1730677646855554
1107	1	0.42181366132106035
1107	2	0.6049312221166154
1107	3	0.5571072455397328
1107	4	0.8702127216291728
1107	5	0.7772042081433455
1107	6	0.4840364021822623
1107	7	0.4842044016963669
1107	8	0.5552106160648478
1107	9	0.7839220805115588
1107	10	1.012086957959994
1107	11	0.598034550571299
1107	12	0.6518954609186594
1107	13	0.6259945582646154
1107	14	1.4114402284037766
1107	15	0.7863892635138683
1107	16	1.0334287074675146
1107	17	1.4843312780417435
1107	18	1.5666761382898784
1107	19	1.1250745514061222
1107	20	0.8884109790431154
1107	21	0.6586559075767653
1107	22	0.9428238377339042
1107	23	1.100404367866851
1107	24	0.8560988292893441
1107	25	1.2111517507681597
1108	1	0.38973529132424645
1108	2	0.7475941789609486
1108	3	0.5037205835103435
1108	4	1.0749543383001878
1108	5	1.007532005726162
1108	6	0.528655710648195
1108	7	0.362258483950086
1108	8	0.4454926205282277
1108	9	0.5882173591829769
1108	10	0.8077858826911779
1108	11	0.4794129814323824
1108	12	0.270656148395112
1108	13	0.3561513562242169
1108	14	1.326983804324712
1108	15	0.8390484525698909
1108	16	0.9527371733980132
1108	17	1.3167226642980125
1108	18	1.4833123728202082
1108	19	1.0746296625558784
1108	20	0.7904079068464966
1108	21	0.7567720134226334
1108	22	0.924735555555543
1108	23	1.2456381156569023
1108	24	0.8468416632458116
1108	25	1.2144326248950463
1109	1	0.28340991880620336
1109	2	0.5948923695469936
1109	3	0.5287522619865967
1109	4	0.8552792107812976
1109	5	0.9179714131823928
1109	6	0.5323430095206518
1109	7	0.4999632575981394
1109	8	0.5772508375177452
1109	9	0.7725085284170703
1109	10	1.108224740864415
1109	11	0.6288830180449381
1109	12	0.5315718220907044
1109	13	0.5604814109062843
1109	14	1.4907923053314107
1109	15	0.9637493300968316
1109	16	1.0523386591138149
1109	17	1.474827145500221
1109	18	1.7804041087628235
1109	19	0.9877955971608969
1109	20	0.9212656785379707
1109	21	0.7295406459542022
1109	22	1.0216209315586937
1109	23	1.3278705153985513
1109	24	1.1175288920373347
1109	25	1.3017261939276068
1110	1	0.2512919753449836
1110	2	0.5353728726082778
1110	3	0.49146596418226807
1110	4	0.7447307854571221
1110	5	0.6364638977750374
1110	6	0.32303065815178655
1110	7	0.3544470183072903
1110	8	0.36462722345606835
1110	9	0.4848705440600227
1110	10	0.9109180141948627
1110	11	0.4976075605286354
1110	12	0.40561242155581567
1110	13	0.32408576667708644
1110	14	1.0370157079049989
1110	15	0.8995852470359509
1110	16	1.001630086418409
1110	17	1.2416261064964402
1110	18	0.7882462522585527
1110	19	0.5139979118736022
1110	20	0.4249192951861005
1110	21	0.33946378725315896
1110	22	0.6374137052333069
1110	23	-0.6797778338554323
1110	24	0.12077568362366975
1110	25	0.5437006363239547
1111	1	0.24585734650211677
1111	2	0.6554928230880321
1111	3	0.6517477998264454
1111	4	0.9194525208373321
1111	5	0.8688254651412279
1111	6	0.4951025137386438
1111	7	0.5441449761707347
1111	8	0.6138866373492675
1111	9	0.6632435945254045
1111	10	0.9670833648587518
1111	11	0.7329643532746388
1111	12	0.5600474694396596
1111	13	0.45092729119098607
1111	14	1.3952522527133269
1111	15	0.9807052334553532
1111	16	1.1278459169912693
1111	17	1.3997979217085401
1111	18	1.7181967281912307
1111	19	1.1140687015058575
1111	20	0.767752643048226
1111	21	0.647895247289803
1111	22	0.977104245765581
1111	23	1.367249383926584
1111	24	1.1072353264600565
1111	25	1.2514811108496318
1112	1	0.11398095733258629
1112	2	0.19270678045315748
1112	3	0.12368026092969941
1112	4	0.4565780765051457
1112	5	0.38686256859647017
1112	6	0.10879341088958228
1112	7	-0.04271600584583268
1112	8	0.10456657268974112
1112	9	0.19907153956480528
1112	10	0.502191467505746
1112	11	0.005961423389813092
1112	12	0.03995818372537485
1112	13	-0.03700271992236992
1112	14	0.4649412050269248
1112	15	0.26388112757537385
1112	16	0.19562791840569815
1112	17	0.7906912840039553
1112	18	0.90939381346098
1112	19	0.3242216364422106
1112	20	0.13829782894436704
1112	21	0.07236546854662151
1112	22	0.3711421827225436
1112	23	0.725336244908604
1112	24	0.52842837050936
1112	25	0.6590192498676117
1113	1	0.25546982560716813
1113	2	0.3382135058705984
1113	3	0.3115861050855422
1113	4	0.6386919447797297
1113	5	0.5976870120101978
1113	6	0.4386403496650928
1113	7	0.4064143281059316
1113	8	0.39042000497174456
1113	9	0.5887515024590542
1113	10	0.8387522281736537
1113	11	0.4756390671745976
1113	12	0.41613513177732386
1113	13	0.3142438014134328
1113	14	0.9735624944517909
1113	15	0.7457688826774174
1113	16	0.7793535677563455
1113	17	1.0737154027423585
1113	18	1.069232635353373
1113	19	0.7266161169616865
1113	20	0.5830276526920528
1113	21	0.5089784091774163
1113	22	0.6182664869897039
1113	23	0.8241013191201034
1113	24	0.7161196970399715
1113	25	1.2055057990327356
1114	1	-0.9339659674327443
1114	2	-0.6762958406181525
1114	3	-0.6628002065433307
1114	4	-0.43260066421311194
1114	5	-3.3027528227545773
1114	6	-1.2849948252286651
1114	7	-1.0522560632875724
1114	8	-0.6500217614997723
1114	9	-0.47666574619617563
1114	10	-0.2428772407555897
1114	11	-0.6394423616940159
1114	12	-0.8472241062386701
1114	13	-0.8082828709988235
1114	14	-6.131859657766704
1114	15	-2.634968286332061
1114	16	-1.4488790691719444
1114	17	-0.4794191629208058
1114	18	-6.723111119290152
1114	19	-2.682515655156351
1114	20	-1.715476017550749
1114	21	-1.2865979823943974
1114	22	-0.9824222407585239
1114	23	-0.5952684987050887
1114	24	-0.7235337844899377
1114	25	-0.4830220918406951
1115	1	-0.7156854579581875
1115	2	-0.4210198866361772
1115	3	-0.49051377345318414
1115	4	-3.0836265640495704
1115	5	-1.0396856872330034
1115	6	-0.7494097297518962
1115	7	-0.5663129446213446
1115	8	-0.43409414993809037
1115	9	-0.2272416770233292
1115	10	-0.5778832945769778
1115	11	-0.6755451802326675
1115	12	-0.6273503395858455
1115	13	-0.6348172095180757
1115	14	-5.9505662544971045
1115	15	-2.357237283817959
1115	16	-1.2836287736432581
1115	17	-0.26329057688951707
1115	18	-0.017287499188341826
1115	19	-0.39340864245156126
1115	20	-0.4413533619767307
1115	21	-0.5623520589156428
1115	22	-1.7689637288376332
1115	23	-0.8134886089374476
1115	24	-0.5309885903560811
1115	25	-0.10967919582851948
1116	1	0.2970989625992111
1116	2	0.45949414055849047
1116	3	0.3451664360449058
1116	4	0.5250647453662367
1116	5	-0.2494524412784429
1116	6	0.25263804551762803
1116	7	0.3429612489270397
1116	8	0.3916283732164061
1116	9	0.5395710458189242
1116	10	0.6970667966696737
1116	11	0.3290049553187089
1116	12	0.2218418242783713
1116	13	0.2679985144423398
1116	14	1.3937800792863086
1116	15	0.8388276384377846
1116	16	0.9294268815393352
1116	17	1.3523889469400956
1116	18	1.1676537706696664
1116	19	0.9953765903592569
1116	20	0.49123355968828264
1116	21	0.40081963247025243
1116	22	0.5149765309779681
1116	23	-0.2711149565397193
1116	24	0.22326255418283125
1116	25	0.8621236771223906
1201	1	0.84620926278388
1201	2	0.9681560055146825
1201	3	0.9852874037868418
1201	4	1.2383403779543514
1201	5	1.2056690421603165
1201	6	0.9487673724595922
1201	7	0.9129805998366365
1201	8	1.0028426919072189
1201	9	1.1481117220202464
1201	10	1.6188509357997631
1201	11	1.0556076673928914
1201	12	1.0214893086601222
1201	13	0.8731883891034542
1201	14	1.676323618832889
1201	15	1.3900572070205541
1201	16	1.537885291817915
1201	17	1.8158690164855003
1201	18	1.1611940539591288
1201	19	1.1378010039382929
1201	20	0.9748122992270041
1201	21	0.8844106505049112
1201	22	1.2355652357439908
1201	23	1.5653121484856243
1201	24	1.299693735668825
1201	25	1.4854372257059367
1202	1	0.7246190635625105
1202	2	0.8636848777064223
1202	3	0.8174238490292112
1202	4	1.1771922984057568
1202	5	0.9719383740533019
1202	6	0.6193020577953803
1202	7	0.6934117711177308
1202	8	0.8246107842702415
1202	9	1.0526336556141018
1202	10	1.1807999171692742
1202	11	0.8416721214931115
1202	12	0.7204491750051858
1202	13	0.7627488570998224
1202	14	1.668604346674968
1202	15	1.2287965820875257
1202	16	1.350367114353375
1202	17	1.7016986851690916
1202	18	1.7704130079430769
1202	19	1.3203998765892564
1202	20	1.0497592155636468
1202	21	0.8822944109494344
1202	22	1.1061520753690033
1202	23	1.3412181789209825
1202	24	1.0983373596885144
1202	25	1.471146367321417
1203	1	0.46664533049264634
1203	2	0.6047499657510649
1203	3	0.42612302440288374
1203	4	0.7723960951581716
1203	5	0.6950983228707344
1203	6	0.4407664334014214
1203	7	0.41157665586661096
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2211	14	0.21679319276204723
2211	15	0.08259787375977368
2211	16	-6.6566818166878505
2211	17	-1.8213818806352151
2211	18	-0.5843117700576173
2211	19	-0.5107445945422313
2211	20	-0.5605861386573565
2211	21	-0.49942023012697234
2211	22	-0.4197022456240731
2211	23	0.07403177422191476
2211	24	-0.4067864054254926
2211	25	-7.878266395724772
2212	1	-1.5454554333034416
2212	2	-4.164634915573959
2212	3	-1.9269905644275553
2212	4	-3.7923450534355183
2212	5	-1.4211370744673602
2212	6	-1.122290959599031
2212	7	-1.0304762353661232
2212	8	-0.8295155051398311
2212	9	-1.019146434754866
2212	10	-0.40982118345678487
2212	11	-0.8630749999984886
2212	12	-0.9666060830689105
2212	13	-1.1092174055005586
2212	14	-6.564170059687747
2212	15	-5.222132777830975
2212	16	-2.950378152688991
2212	17	-7.731579465991274
2212	18	-3.290981029137324
2212	19	-1.9509440526910353
2212	20	-1.9411108295798059
2212	21	-1.6311305781269567
2212	22	-1.5060805200076643
2212	23	-0.9989566995206474
2212	24	-1.2275788294257666
2212	25	-1.074292394360036
2213	1	-0.11120525372662726
2213	2	0.02399606490338968
2213	3	-0.08145152467398376
2213	4	0.3216032035426437
2213	5	0.14472903780725943
2213	6	-0.09802292512144106
2213	7	-0.290530968777432
2213	8	-0.27750028316378206
2213	9	0.042195634975655594
2213	10	-3.6707969869345582
2213	11	-1.285645134842028
2213	12	-0.7427262221057092
2213	13	-0.5654395069165119
2213	14	0.3821729765680004
2213	15	-0.3135805399655922
2213	16	-0.04845126909153663
2213	17	0.39868342790394706
2213	18	0.6180263393266969
2213	19	0.020125526412563488
2213	20	-0.41519844762448344
2213	21	-0.28117394388915073
2213	22	-0.6574120231034897
2213	23	-0.9170688996360568
2213	24	-0.37777491871899826
2213	25	-0.03026207972833106
2214	1	-0.7709912133538701
2214	2	-0.5093424104353197
2214	3	-0.4973514851020475
2214	4	-0.15537371178592707
2214	5	-3.3333916315684426
2214	6	-1.323598106323363
2214	7	-1.0161235506819537
2214	8	-0.7770493312475892
2214	9	-0.6232975595900072
2214	10	-4.47176364484222
2214	11	-1.7673263007907707
2214	12	-1.2249904093482158
2214	13	-0.8840155780959407
2214	14	-0.15177119963190933
2214	15	-0.38365932800079605
2214	16	-0.33068431103153273
2214	17	-0.09350140822444786
2214	18	0.18865002352075777
2214	19	-0.5160786882331152
2214	20	-0.8152587830943858
2214	21	-0.7938895561905497
2214	22	-0.5198016948284803
2214	23	-0.3040552465364641
2214	24	-0.669098556928283
2214	25	-0.43201223848529935
2215	1	-2.183981698241631
2215	2	-4.656890272399092
2215	3	-2.862735927386197
2215	4	-1.839080104946305
2215	5	-1.778255451229736
2215	6	-1.9515920362251045
2215	7	-2.1145955110503785
2215	8	-2.2333915528806436
2215	9	-7.630569730423421
2215	10	-3.7895039938933266
2215	11	-2.9593175025032927
2215	12	-3.251986717209725
2215	13	-2.6723028988079243
2215	14	-7.913023343559846
2215	15	-4.596451745042238
2215	16	-9.647860242412747
2215	17	-4.605755142914187
2215	18	-2.9058826738351726
2215	19	-3.3433742458835027
2215	20	-3.033755725951341
2215	21	-3.186173134029861
2215	22	-8.91807210960489
2215	23	-10.3293703712116
2215	24	-6.449290913088717
2215	25	-4.805261091238368
2216	1	-1.437094907796073
2216	2	-1.3669510500703552
2216	3	-1.2906664560179486
2216	4	-6.597617242926972
2216	5	-3.0659574180938556
2216	6	-2.1086533504236407
2216	7	-1.568087567513981
2216	8	-7.5733393722969495
2216	9	-3.287341503913453
2216	10	-1.6031490382617761
2216	11	-1.532941637834675
2216	12	-1.6218236485727644
2216	13	-1.5817148982719687
2216	14	-0.6810353921336798
2216	15	-1.1874831137733395
2216	16	-0.8122331064390238
2216	17	-0.6663850247804248
2216	18	-6.548334114666794
2216	19	-3.7881757681718113
2216	20	-3.2420953609402794
2216	21	-2.2564527435208177
2216	22	-1.5939199290787016
2216	23	-1.0057667941331019
2216	24	-1.523631855755056
2216	25	-0.916263580655901
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	92883.0
3	2.0
4	794.0
5	4594.0
6	5452.0
7	5092.0
8	4595.0
9	4095.0
10	4094.0
11	3856.0
12	3645.0
13	3611.0
14	3764.0
15	3752.0
16	3756.0
17	3935.0
18	4607.0
19	5754.0
20	7263.0
21	9257.0
22	11501.0
23	13644.0
24	15764.0
25	17882.0
26	20228.0
27	23038.0
28	25376.0
29	30348.0
30	36946.0
31	44040.0
32	56388.0
33	73789.0
34	98288.0
35	133419.0
36	192382.0
37	284191.0
38	783355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.16366197224687	18.804254161074848	12.60387000507385	25.428213861604434
2	14.335144143332235	20.319927823684132	41.71486710959365	23.63006092338998
3	18.36200888954555	24.98523744444034	29.891232347110897	26.761521318903213
4	11.393954813709234	16.862293790274904	39.43852673422767	32.30522466178819
5	12.534829915758142	38.125818115200474	36.53065968279045	12.808692286250936
6	31.43172331104903	37.62287691056551	18.325311476537603	12.620088301847854
7	27.596034544181418	31.616377774774882	22.775573381816393	18.012014299227303
8	24.742393166257017	36.84571303900883	21.053247099128384	17.35864669560577
9	26.342373560970433	14.952099504091295	20.33269911530516	38.37282781963311
10	13.752520864700818	28.97367883102639	35.99323052005488	21.280569784217914
11	34.23288355822089	22.538730634682658	24.769965066630892	18.45842074046556
12	22.738845896979623	24.911948081433835	32.147611725316345	20.201594296270194
13	28.63555388467483	21.9107012538977	25.983866172282543	23.469878689144927
14	20.319657815566757	21.797008834719296	28.893777955562022	28.98955539415192
15	23.01168032746597	30.375781752265386	23.669964394760072	22.94257352550857
16	21.991045704645845	28.117234340425384	27.882050180810726	22.009669774118045
17	21.461351273906168	28.348706777700826	28.308108498655	21.881833449738004
18	21.291346709095603	27.63322726099937	29.783538720497354	21.291887309407674
19	23.16607268874443	27.210020502558397	27.72115930861363	21.902747500083546
20	23.403316350726623	27.462236347365156	27.87322951779193	21.26121778411629
21	23.09119834237553	26.90779414749653	27.255311421380547	22.745696088747387
22	22.924726531876146	27.827909845835492	27.353595328616404	21.893768293671954
23	22.17305666074321	27.57424633191275	27.905524147258177	22.347172860085866
24	22.844321841069256	27.220046569402122	27.9564860697322	21.97914551979642
25	22.68653312704089	27.245150522054878	27.884866797860276	22.18344955304396
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	157.0
1	157.0
2	285.0
3	413.0
4	413.0
5	413.0
6	907.0
7	1401.0
8	1401.0
9	1401.0
10	2340.0
11	3279.0
12	3279.0
13	3279.0
14	6256.5
15	9234.0
16	9234.0
17	9234.0
18	17046.0
19	24858.0
20	24858.0
21	24858.0
22	39464.5
23	54071.0
24	54071.0
25	54071.0
26	76276.5
27	98482.0
28	98482.0
29	98482.0
30	126087.5
31	153693.0
32	153693.0
33	153693.0
34	176174.0
35	198655.0
36	198655.0
37	198655.0
38	216264.0
39	233873.0
40	233873.0
41	233873.0
42	245297.5
43	256722.0
44	256722.0
45	256722.0
46	262840.5
47	268959.0
48	268959.0
49	268959.0
50	256527.5
51	244096.0
52	244096.0
53	244096.0
54	217694.5
55	191293.0
56	191293.0
57	191293.0
58	162730.0
59	134167.0
60	134167.0
61	134167.0
62	108927.0
63	83687.0
64	83687.0
65	83687.0
66	64401.0
67	45115.0
68	45115.0
69	45115.0
70	32800.5
71	20486.0
72	20486.0
73	20486.0
74	14386.5
75	8287.0
76	8287.0
77	8287.0
78	5655.5
79	3024.0
80	3024.0
81	3024.0
82	2006.0
83	988.0
84	988.0
85	988.0
86	661.5
87	335.0
88	335.0
89	335.0
90	206.5
91	78.0
92	78.0
93	78.0
94	49.0
95	20.0
96	20.0
97	20.0
98	13.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16670105827904372
2	0.2356808065324411
3	0.24098694101347168
4	0.35801668484509036
5	0.23572993740726544
6	0.015476225569672493
7	0.014984916821428922
8	0.01483752419695585
9	0.016114926942389137
10	0.017883638436065993
11	0.017834507561241636
12	0.02461456828700292
13	0.025793709282787493
14	0.022305417170258135
15	0.04166298185105484
16	0.01862060155843135
17	0.03984513948255363
18	0.029724179268736058
19	0.02515500791007085
20	0.02716937377786949
21	0.032475508258900054
22	0.042498206723068914
23	0.02613762540655799
24	0.04898348219988405
25	0.035767276872131985
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2035380.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.32944504303605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.17510537166308	65.01368524501162
2	10.434482232712888	15.92916476268789
3	2.43066649544582	5.565942740462423
4	0.8388785362473278	2.5612453252109173
5	0.3725867095471651	1.4219668385072988
6	0.20458995780199413	0.9369742764242625
7	0.12823107721612212	0.6851464872824112
8	0.07794699257036908	0.47597205485359406
9	0.06077289156522489	0.4174884978150799
>10	0.25110620901062974	3.2672787282196936
>50	0.013603186993275281	0.7071476948329508
>100	0.010210809785660548	1.583666221759274
>500	0.0011047143030702212	0.5438287801531481
>1k	7.148151372807022E-4	0.8904923467793803
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2267	0.11137969322681758	No Hit
GTCCTACAGTGGACATTTCTAAATT	2211	0.1086283642366536	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.826174964871424E-5
2	0.0	0.0	0.0	0.0	9.826174964871424E-5
3	0.0	0.0	0.0	4.913087482435712E-5	9.826174964871424E-5
4	0.0	0.0	0.0	4.913087482435712E-5	9.826174964871424E-5
5	0.0	4.913087482435712E-5	0.0	4.913087482435712E-5	1.4739262447307136E-4
6	4.913087482435712E-5	4.913087482435712E-5	0.0	4.913087482435712E-5	1.4739262447307136E-4
7	4.913087482435712E-5	9.826174964871424E-5	0.0	9.826174964871424E-5	1.4739262447307136E-4
8	9.826174964871424E-5	9.826174964871424E-5	0.0	9.826174964871424E-5	1.9652349929742848E-4
9	9.826174964871424E-5	9.826174964871424E-5	0.0	1.4739262447307136E-4	1.9652349929742848E-4
10	9.826174964871424E-5	9.826174964871424E-5	0.0	1.9652349929742848E-4	1.9652349929742848E-4
11	9.826174964871424E-5	9.826174964871424E-5	0.0	1.9652349929742848E-4	1.9652349929742848E-4
12	9.826174964871424E-5	9.826174964871424E-5	0.0	2.456543741217856E-4	1.9652349929742848E-4
13	9.826174964871424E-5	9.826174964871424E-5	0.0	2.456543741217856E-4	1.9652349929742848E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATCGA	50	0.0015126036	13.285034	6
TAGGACC	655	0.0	13.082149	4
TCCAACG	125	1.03682396E-10	12.910221	18
GGCGAGG	550	0.0	11.736854	19
GACGTGA	600	0.0	11.546179	7
TTAGGAC	860	0.0	11.292227	3
TGTAGGA	1410	0.0	10.878084	2
GGACGTG	1100	0.0	10.869574	6
AGGCGTG	105	3.511308E-6	10.845725	7
GTCTAGG	220	0.0	10.843265	1
TGGCGAG	1240	0.0	10.794242	18
ACGTGAA	720	0.0	10.675998	8
GTCCTAC	1405	0.0	10.594757	1
GTCCTAA	750	0.0	10.559893	1
CGTGAAA	730	0.0	10.4018	9
AGGACCT	1145	0.0	10.38145	5
GTAGGAC	1350	0.0	10.367199	3
AGGACGT	1135	0.0	10.305349	5
CTTACAC	305	0.0	10.301298	3
ACCGTGC	75	0.00267193	10.122428	8
>>END_MODULE