FastQCFastQC Report
Thu 26 May 2016
SRR1513122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513122_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2035380
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31500.15476225569672494No Hit
TATCAACGCAGAGTACTTTTTTTTT21990.10803879373876132No Hit
CTTTAGGACGTGAAATATGGCGAGG21300.10464876337588068No Hit
GTCCTACAGTGGACATTTCTAAATT20980.10307657538150125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA903.6983693E-813.70582819
TCCAACG1750.013.01620418
CCGACGG658.087812E-411.68149713
ACAACGC600.0059238211.0714518
GTAGGAC13500.010.7898533
GTCCTAC13700.010.6370571
CCGGTTT1252.2695895E-710.63016112
TGTAGGA14750.010.5862422
TAGGACC5150.010.5366874
GCCGGTT1201.5452115E-610.28063411
GGACGTG13550.010.1164366
TGGCGAG13550.010.08635718
CCGTCCT856.666173E-410.0470539
GGCGAGG6550.09.99567819
AGGACGT13850.09.9667765
GGCGTGC1054.154574E-59.9417128
TCCTACA15550.09.9191532
GTCCTAA7050.09.862441
AGGACCT10250.09.8450625
TAGGACT3100.09.8270864
Produced by FastQC (version 0.11.5)