FastQCFastQC Report
Thu 26 May 2016
SRR1513121_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513121_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388446
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT36130.15126990520195976No Hit
GTCCTAAAGTGTGTATTTCTCATTT32970.13803954537804078No Hit
CTGTAGGACGTGGAATATGGCAAGA26600.1113694845937484No Hit
CTTTAGGACGTGAAATATGGCGAGG25260.1057591421367701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC802.028497E-613.04644510
TAGGACC7450.012.4087614
GCGTGCG907.568029E-611.5968399
GCGCCAC1451.2441888E-911.12424213
GGCGAGG7800.011.07063919
GTAGGAC19450.010.7307073
GGACGTG17600.010.7269276
TGGCGAG17750.010.69196518
AGGACGT18050.010.4871695
TGTAGGA22050.010.3804752
TTAGGAC12700.010.3556953
GGACCTG13350.010.0911816
GACGTGA10700.010.019757
AGGACCT14200.09.9811445
GTATTAA3550.09.9574351
GACGTGG8600.09.9290177
ATGGCGA20200.09.86471817
CTGTAGG21850.09.8379631
GTCTTAG3900.09.7987221
GTCCTAC16300.09.7882021
Produced by FastQC (version 0.11.5)