FastQCFastQC Report
Thu 26 May 2016
SRR1513121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513121_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388446
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT35750.14967891256490623No Hit
GTCCTAAAGTGTGTATTTCTCATTT34660.14511527579019998No Hit
GTATCAACGCAGAGTACTTTTTTTT32680.13682536678660517No Hit
CTTTAGGACGTGAAATATGGCGAGG31870.13343404037604367No Hit
CTGTAGGACGTGGAATATGGCAAGA30250.12665138755492064No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCGC400.005329323514.23012210
TAGGACC6800.012.18264
GGCGAGG7900.011.28802919
TCGAACT1354.836693E-911.24355219
AACCGCT700.001505959510.844957
TATAGCG803.6923675E-410.7122875
AGGACGT19300.010.5087725
GGACGTG19150.010.4905376
AATCCCG1909.094947E-1210.48535319
GACGTGG8100.010.3093977
ACGAAAT1409.8340024E-810.16863112
GACGTGA11400.010.1552497
TCGTCAA1502.4998371E-810.11919810
AGGACCT13700.010.0780645
GTAGGAC19350.010.0391543
TGGCGAG19500.09.97721618
TGTAGGA20950.09.9102432
CGTGGAA8150.09.89436159
TTAGGAC13200.09.8831313
GGACCTG13300.09.8789666
Produced by FastQC (version 0.11.5)